##################################
# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII AccI AccII AcsI AcyI
AflIII AgeI AlwNI AocI Aor51HI
ApaI ApoI AscI AseI AsnI
AspI AvaI AviII AvrII BbeI
BbvCI BciVI BclI BlnI BlpI
Bpu1102I BsaBI BsaHI BsiEI BsiWI
BsmBI BsoBI BspDI BspHI BspLU11I
BsrFI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraII DraIII DrdI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV FbaI FseI
FspI HaeII Hin1I HindIII HpaI
KasI KspI MamI MluI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfoI SfuI SgrAI SmaI SnaBI
SphI Sse8387I SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
Acc65I 1742 GGTACC G^GTACC
AccIII 127 TCCGGA T^CCGGA
AclI 1697 AACGTT AA^CGTT
AflII 2157 CTTAAG C^TTAAG
AhdI 1448 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 121 GTGCAC G^TGCAC
ApaLI 121 GTGCAC G^TGCAC
Asp700I 390 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1742 GGTACC G^GTACC
AspEI 1448 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 801 TGGCCA TGG^CCA
BamHI 1585 GGATCC G^GATCC
BbrPI 1275 CACGTG CAC^GTG
BcgI 53 CGAGCCCTGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 113 CCGGG CC^[GC]GG
BfrI 2157 CTTAAG C^TTAAG
BglII 893 AGATCT A^GATCT
BsaAI 1275 CACGTG [CT]AC^GT[GA]
BsaI 1065 GGTCTC GGTCTC(1/5)
BseAI 127 TCCGGA T^CCGGA
BsmI 2224 GCATTC GAATGC(1/-1)
Bsp1407I 426 TGTACA T^GTACA
BspEI 127 TCCGGA T^CCGGA
BsrBI 530 GAGCGG CCGCTC(-3/-3)
BsrDI 705 CATTGC GCAATG(2/0)
BsrGI 426 TGTACA T^GTACA
BstAPI 1161 GCAGAAAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 344 CCACTGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 92 GCGC GCG^C
DsaI 1718 CCATGG C^C[GA][CT]GG
EaeI 801 TGGCCA [CT]^GGCC[GA]
Eam1105I 1448 GACCATGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 853 ATGCAT ATGCA^T
HgaI 686 GACGC GACGC(5/10)
HhaI 92 GCGC GCG^C
HinP1I 92 GCGC G^CGC
HincII 809 GTTGAC GT[CT]^[GA]AC
HindII 809 GTTGAC GT[CT]^[GA]AC
KpnI 1742 GGTACC GGTAC^C
MfeI 874 CAATTG C^AATTG
MluNI 801 TGGCCA TGG^CCA
MroI 127 TCCGGA T^CCGGA
MscI 801 TGGCCA TGG^CCA
MunI 874 CAATTG C^AATTG
NciI 113 CCGGG CC^[GC]GG
NcoI 1718 CCATGG C^CATGG
NheI 1531 GCTAGC G^CTAGC
NsiI 853 ATGCAT ATGCA^T
PmaCI 1275 CACGTG CAC^GTG
PmlI 1275 CACGTG CAC^GTG
Psp1406I 1697 AACGTT AA^CGTT
PvuII 1483 CAGCTG CAG^CTG
SfiI 793 GGCCCTGGTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SpeI 2022 ACTAGT A^CTAGT
SspBI 426 TGTACA T^GTACA
SspI 336 AATATT AAT^ATT
TfiI 1412 GAATC G^A[AT]TC
XmnI 390 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 10 enzymes) #
###################################
AspHI 121 GTGCAC G[AT]GC[AT]^C
628 GTGCTC
BanI 901 GGCACC G^G[CT][GA]CC
1742 GGTACC
BbsI 439 GAAGAC GAAGAC(2/6)
2003 GTCTTC
BglI 213 GCCTGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
794 GCCCTGGTGGC
BpuAI 439 GAAGAC GAAGAC(2/6)
2003 GTCTTC
BsiHKAI 121 GTGCAC G[AT]GC[AT]^C
628 GTGCTC
MspA1I 1483 CAGCTG C[AC]G^C[GT]G
1687 CAGCGG
SapI 393 GCTCTTC GCTCTTC(1/4)
1769 GCTCTTC
TaqI 1614 TCGA T^CGA
1884 TCGA
TthHB8I 1614 TCGA T^CGA
1884 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaII
BanII BbvI BfaI BmyI BpmI
BsaJI BsaWI BseRI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI PstI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI Tru9I
TseI Tsp45I Tsp509I TspRI XhoII