##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BbeI BbrPI
BfrI BlnI BsaAI BsaHI BsaI
BsaWI BseAI BsiEI BsiWI BsmBI
BsoBI BspDI BspEI BspHI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FspI Hin1I HincII HindII HindIII
HpaI KasI KpnI KspI MaeII
MfeI MluI MroI MunI NarI
NheI NotI NruI NsiI PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgrAI SmaI
SnaBI SpeI Sse8387I SspI SwaI
Tth111I XcmI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccI 1929 GTAGAC GT^[AC][GT]AC
AocI 734 CCTCAGG CC^T[ACGT]AGG
Asp700I 107 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 617 GGATCC G^GATCC
BbvCI 200 GCTGAGG CCTCAGC(-5/-2)
BcgI 356 GCACCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 91 TGATCA T^GATCA
BglII 1790 AGATCT A^GATCT
BsaBI 2116 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsmI 1050 GCATTC GAATGC(1/-1)
Bsp1407I 2151 TGTACA T^GTACA
BspMI 577 ACCTGC ACCTGC(4/8)
BsrFI 31 GCCGGC [GA]^CCGG[CT]
BsrGI 2151 TGTACA T^GTACA
BssSI 583 CTCGTG CACGAG(-5/-1)
BstBI 364 TTCGAA TT^CGAA
Bsu36I 734 CCTCAGG CC^T[ACGT]AGG
Cfr10I 31 GCCGGC [GA]^CCGG[CT]
Eco81I 734 CCTCAGG CC^T[ACGT]AGG
FbaI 91 TGATCA T^GATCA
FseI 30 GGCCGGCC GGCCGG^CC
HaeII 568 GGCGCT [GA]GCGC^[CT]
HgaI 1122 GACGC GACGC(5/10)
MamI 2116 GATGTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 31 GCCGGC GCC^GGC
NcoI 787 CCATGG C^CATGG
NdeI 2190 CATATG CA^TATG
NgoMIV 31 GCCGGC G^CCGGC
NspI 2225 GCATGC [GA]CATG^[CT]
NspV 364 TTCGAA TT^CGAA
SacI 168 GAGCTC GAGCT^C
SapI 1725 GCTCTTC GCTCTTC(1/4)
SfuI 364 TTCGAA TT^CGAA
SphI 2225 GCATGC GCATG^C
SspBI 2151 TGTACA T^GTACA
TaqI 365 TCGA T^CGA
TfiI 1074 GAATC G^A[AT]TC
Tsp509I 1748 AATT ^AATT
TthHB8I 365 TCGA T^CGA
XbaI 1525 TCTAGA T^CTAGA
XmnI 107 GAAACTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 651 CGCG CG^CG
726 CGCG
AspHI 168 GAGCTC G[AT]GC[AT]^C
555 GTGCTC
BalI 1546 TGGCCA TGG^CCA
1556 TGGCCA
BbsI 1119 GAAGAC GAAGAC(2/6)
1818 GAAGAC
BcnI 309 CCGGG CC^[GC]GG
839 CCCGG
BpuAI 1119 GAAGAC GAAGAC(2/6)
1818 GAAGAC
BseRI 246 GAGGAG GAGGAG(10/8)
2314 GAGGAG
BsiHKAI 168 GAGCTC G[AT]GC[AT]^C
555 GTGCTC
BsrBI 701 CCGCTC CCGCTC(-3/-3)
432 GAGCGG
BsrDI 870 CATTGC GCAATG(2/0)
1098 CATTGC
BstUI 651 CGCG CG^CG
726 CGCG
DsaI 406 CCGTGG C^C[GA][CT]GG
787 CCATGG
HphI 224 GGTGA GGTGA(8/7)
2321 GGTGA
MluNI 1546 TGGCCA TGG^CCA
1556 TGGCCA
MscI 1546 TGGCCA TGG^CCA
1556 TGGCCA
MspA1I 163 CAGCTG C[AC]G^C[GT]G
832 CAGCTG
MvnI 651 CGCG CG^CG
726 CGCG
NciI 309 CCGGG CC^[GC]GG
839 CCCGG
PstI 277 CTGCAG CTGCA^G
2349 CTGCAG
PvuII 163 CAGCTG CAG^CTG
832 CAGCTG
SmlI 1462 CTCAAG C^T[CT][GA]AG
2248 CTTGAG
StuI 77 AGGCCT AGG^CCT
939 AGGCCT
Tsp45I 97 GTGAC ^GT[GC]AC
513 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 84 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
ApaI AvaII BanI BanII BbvI
BciVI BfaI BglI BlpI BmyI
BpmI Bpu1102I BsaJI BsgI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrI
BssKI BstAPI BstF5I BstNI BstXI
BstYI Cac8I CelII CfoI Cfr13I
DdeI DpnII DraII EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspI MvaI MwoI
NdeII NlaIII NlaIV PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfiI StyI Tru9I
TseI TspRI Van91I XhoII