##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AocI ApaI
ApoI AscI AseI AsnI Asp718I
AspI AvrII BalI BamHI BbeI
BclI BfaI BfrI BglII BlnI
BsaBI BsaHI BseAI BsiWI BsmBI
BspDI BspEI BspHI BspLU11I BsrBI
Bst1107I BstAPI BstZ17I Bsu36I ClaI
CpoI DraI DrdI Eco81I EcoRI
EcoRV FbaI FseI Hin1I HindIII
HpaI KasI KpnI MaeI MamI
MfeI MluNI MroI MscI MunI
NarI NheI NotI NruI PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI RcaI RsrII SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SpeI Sse8387I SspI SwaI
Tsp509I Tth111I XbaI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AccI 1553 GTCGAC GT^[AC][GT]AC
AhdI 264 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 914 GTGCAC G^TGCAC
Aor51HI 1338 AGCGCT AGC^GCT
ApaLI 914 GTGCAC G^TGCAC
Asp700I 628 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 264 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 763 TGCGCA TGC^GCA
BanI 1877 GGCACC G^G[CT][GA]CC
BcgI 1440 GCATTTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1606 GTATCC GTATCC(6/5)
BlpI 838 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
BsaI 1487 GAGACC GGTCTC(1/5)
BsaWI 789 TCCGGT [AT]^CCGG[AT]
BsmI 366 GAATGC GAATGC(1/-1)
Bsp1407I 1825 TGTACA T^GTACA
BspMI 819 ACCTGC ACCTGC(4/8)
BsrDI 919 CATTGC GCAATG(2/0)
BsrFI 688 GCCGGC [GA]^CCGG[CT]
BsrGI 1825 TGTACA T^GTACA
BssSI 1106 CACGAG CACGAG(-5/-1)
BstBI 187 TTCGAA TT^CGAA
BstXI 1590 CCAACTATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 838 GCTGAGC GC^T[ACGT]AGC
Cfr10I 688 GCCGGC [GA]^CCGG[CT]
DraIII 1892 CACCGAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 264 GACACAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1955 CTCTTC CTCTTC(1/4)
Eco47III 1338 AGCGCT AGC^GCT
EcoT22I 1575 ATGCAT ATGCA^T
FspI 763 TGCGCA TGC^GCA
HincII 1553 GTCGAC GT[CT]^[GA]AC
HindII 1553 GTCGAC GT[CT]^[GA]AC
Ksp632I 1955 CTCTTC CTCTTC(1/4)
KspI 1515 CCGCGG CCGC^GG
MluI 1722 ACGCGT A^CGCGT
MseI 2010 TTAA T^TAA
NaeI 688 GCCGGC GCC^GGC
NcoI 21 CCATGG C^CATGG
NdeI 1578 CATATG CA^TATG
NgoMIV 688 GCCGGC G^CCGGC
NsiI 1575 ATGCAT ATGCA^T
NspV 187 TTCGAA TT^CGAA
PaeR7I 665 CTCGAG C^TCGAG
PshAI 1489 GACCGCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 6 CAGCTG CAG^CTG
SacII 1515 CCGCGG CCGC^GG
SalI 1553 GTCGAC G^TCGAC
SfuI 187 TTCGAA TT^CGAA
SnaBI 1058 TACGTA TAC^GTA
SphI 1573 GCATGC GCATG^C
SspBI 1825 TGTACA T^GTACA
TfiI 1524 GAATC G^A[AT]TC
Tru9I 2010 TTAA T^TAA
XhoI 665 CTCGAG C^TCGAG
XmnI 628 GAATAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwI 384 GGATC GGATC(4/5)
1871 GGATC
BbsI 331 GAAGAC GAAGAC(2/6)
943 GAAGAC
BbvCI 739 CCTCAGC CCTCAGC(-5/-2)
1406 GCTGAGG
BglI 1096 GCCCTTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1362 GCCAGGGAGGC
BpuAI 331 GAAGAC GAAGAC(2/6)
943 GAAGAC
BsiEI 686 CGGCCG CG[GA][CT]^CG
1435 CGGCCG
BsmAI 1487 GAGAC GTCTC(1/5)
1769 GAGAC
BssHII 1045 GCGCGC G^CGCGC
1047 GCGCGC
BstEII 163 GGTGACC G^GT[ACGT]ACC
271 GGTCACC
BstPI 163 GGTGACC G^GT[ACGT]ACC
271 GGTCACC
BstYI 384 GGATCT [GA]^GATC[CT]
1871 GGATCT
DsaI 21 CCATGG C^C[GA][CT]GG
1515 CCGCGG
EagI 686 CGGCCG C^GGCCG
1435 CGGCCG
EclXI 686 CGGCCG C^GGCCG
1435 CGGCCG
Eco52I 686 CGGCCG C^GGCCG
1435 CGGCCG
EcoNI 661 CCTCCTCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
664 CCTCGAGGAGG
EcoO65I 163 GGTGACC G^GT[ACGT]ACC
271 GGTCACC
HaeII 856 GGCGCT [GA]GCGC^[CT]
1338 AGCGCT
MaeIII 164 GTGAC ^GT[ACGT]AC
272 GTCAC
MflI 384 GGATCT [GA]^GATC[CT]
1871 GGATCT
NspI 1573 GCATGC [GA]CATG^[CT]
1822 GCATGT
SacI 831 GAGCTC GAGCT^C
841 GAGCTC
StuI 585 AGGCCT AGG^CCT
603 AGGCCT
Tsp45I 164 GTGAC ^GT[GC]AC
272 GTCAC
XcmI 12 CCACTTGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
21 CCATGGCTGGGCTGG
XhoII 384 GGATCT [GA]^GATC[CT]
1871 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AflIII AluI
AlwNI AspHI AvaI AvaII BanII
BbrPI BbvI BcnI BmyI BpmI
BsaAI BsaJI BseRI BsgI BsiHKAI
BsiYI BslI BsmFI BsoBI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII EaeI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MboI MboII
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PmaCI PmlI
PpuMI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TseI TspRI TthHB8I Van91I