################################## # NO CUT ENZYMES ( 114 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AgeI AhdI Aor51HI AscI AseI AsnI Asp700I Asp718I AspEI AviII AvrII BamHI BanI BbeI BclI BglII BlnI BsaBI BsaI BsaWI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrDI BsrGI BssHII Bst1107I BstBI BstEII BstPI BstUI BstZ17I CfoI ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoNI EcoO65I EcoT22I FauI FbaI FseI FspI HaeII HhaI HinP1I HindIII HpaI KasI KpnI KspI MamI MluI MroI MvnI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SnaBI SphI Sse8387I SspBI SspI StuI SwaI XbaI XcmI XhoI XmnI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### AcyI 839 GACGCC G[GA]^CG[CT]C AflII 623 CTTAAG C^TTAAG AflIII 572 ACGTGT A^C[GA][CT]GT AocI 867 CCTCAGG CC^T[ACGT]AGG ApaI 651 GGGCCC GGGCC^C AspI 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC BbrPI 674 CACGTG CAC^GTG BbvCI 1102 CCTCAGC CCTCAGC(-5/-2) BcgI 74 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 1546 GTATCC GTATCC(6/5) BfrI 623 CTTAAG C^TTAAG BsaHI 839 GACGCC G[GA]^CG[CT]C BsgI 1134 CTGCAC GTGCAG(16/14) BsrFI 143 ACCGGC [GA]^CCGG[CT] BssSI 399 CACGAG CACGAG(-5/-1) BstAPI 1136 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstYI 1352 AGATCC [GA]^GATC[CT] Bsu36I 867 CCTCAGG CC^T[ACGT]AGG Cfr10I 143 ACCGGC [GA]^CCGG[CT] Eco81I 867 CCTCAGG CC^T[ACGT]AGG EcoRI 1268 GAATTC G^AATTC EcoRV 1650 GATATC GAT^ATC Hin1I 839 GACGCC G[GA]^CG[CT]C MfeI 1896 CAATTG C^AATTG MflI 1352 AGATCC [GA]^GATC[CT] MseI 624 TTAA T^TAA MunI 1896 CAATTG C^AATTG NheI 386 GCTAGC G^CTAGC PflFI 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 158 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 674 CACGTG CAC^GTG PmlI 674 CACGTG CAC^GTG SapI 453 GCTCTTC GCTCTTC(1/4) SmaI 268 CCCGGG CCC^GGG SpeI 950 ACTAGT A^CTAGT Tru9I 624 TTAA T^TAA Tth111I 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 158 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XhoII 1352 AGATCC [GA]^GATC[CT] XmaCI 268 CCCGGG C^CCGGG XmaI 268 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 24 enzymes) # ################################### AcsI 253 AAATTC [GA]^AATT[CT] 1268 GAATTC Alw44I 329 GTGCAC G^TGCAC 1073 GTGCAC AlwI 1353 GATCC GGATC(4/5) 1431 GATCC ApaLI 329 GTGCAC G^TGCAC 1073 GTGCAC ApoI 253 AAATTC [GA]^AATT[CT] 1268 GAATTC AvaI 45 CCCGAG C^[CT]CG[GA]G 268 CCCGGG BbsI 1857 GAAGAC GAAGAC(2/6) 284 GTCTTC BglI 877 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1483 GCCTCCAGGGC BlpI 412 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC Bpu1102I 412 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC BpuAI 1857 GAAGAC GAAGAC(2/6) 284 GTCTTC BsaAI 571 TACGTG [CT]AC^GT[GA] 674 CACGTG BsmBI 1522 CGTCTC CGTCTC(1/5) 1497 GAGACG BsmFI 140 GGGAC GGGAC(10/14) 1122 GGGAC BsoBI 45 CCCGAG C^[CT]CG[GA]G 268 CCCGGG CelII 412 GCTCAGC GC^T[ACGT]AGC 582 GCTGAGC DraII 76 AGGACCC [GA]G^G[ACGT]CC[CT] 1168 GGGTCCT EcoO109I 76 AGGACCC [GA]G^G[ACGT]CC[CT] 1168 GGGTCCT HgaI 839 GACGC GACGC(5/10) 404 GCGTC HincII 946 GTCAAC GT[CT]^[GA]AC 1472 GTCAAC HindII 946 GTCAAC GT[CT]^[GA]AC 1472 GTCAAC MspA1I 580 CAGCTG C[AC]G^C[GT]G 1146 CAGCTG PpuMI 76 AGGACCC [GA]G^G[AT]CC[CT] 1168 GGGTCCT PvuII 580 CAGCTG CAG^CTG 1146 CAGCTG ######################################### # 3 OR MORE CUT ENZYMES ( 79 enzymes) # ######################################### AciI AfaI AluI AlwNI AspHI AvaII BalI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstXI Cac8I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MslI MspI MvaI MwoI NciI NcoI NdeII NlaIII NlaIV PleI PstI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I