##################################
# NO CUT ENZYMES (  114 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AgeI        AhdI        Aor51HI     AscI        
AseI        AsnI        Asp700I     Asp718I     AspEI       
AviII       AvrII       BamHI       BanI        BbeI        
BclI        BglII       BlnI        BsaBI       BsaI        
BsaWI       BseAI       BsiEI       BsiWI       BsmI        
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BsrBI       BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstEII      BstPI       BstUI       BstZ17I     
CfoI        ClaI        CpoI        DraI        DraIII      
DrdI        EagI        Eam1105I    EclXI       Eco47III    
Eco52I      EcoNI       EcoO65I     EcoT22I     FauI        
FbaI        FseI        FspI        HaeII       HhaI        
HinP1I      HindIII     HpaI        KasI        KpnI        
KspI        MamI        MluI        MroI        MvnI        
NaeI        NarI        NdeI        NgoMIV      NotI        
NruI        NsiI        NspI        NspV        PacI        
PaeR7I      PinAI       PmeI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfoI        SfuI        SgrAI       SnaBI       SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
XbaI        XcmI        XhoI        XmnI        


###################################
# ONE CUT ENZYMES (   41 enzymes) #
###################################

AcyI          839  GACGCC               G[GA]^CG[CT]C
AflII         623  CTTAAG               C^TTAAG
AflIII        572  ACGTGT               A^C[GA][CT]GT
AocI          867  CCTCAGG              CC^T[ACGT]AGG
ApaI          651  GGGCCC               GGGCC^C
AspI         1700  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI         674  CACGTG               CAC^GTG
BbvCI        1102  CCTCAGC              CCTCAGC(-5/-2)
BcgI           74  CGAGGACCCTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI        1546  GTATCC               GTATCC(6/5)
BfrI          623  CTTAAG               C^TTAAG
BsaHI         839  GACGCC               G[GA]^CG[CT]C
BsgI         1134  CTGCAC               GTGCAG(16/14)
BsrFI         143  ACCGGC               [GA]^CCGG[CT]
BssSI         399  CACGAG               CACGAG(-5/-1)
BstAPI       1136  GCACCAACTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI        1352  AGATCC               [GA]^GATC[CT]
Bsu36I        867  CCTCAGG              CC^T[ACGT]AGG
Cfr10I        143  ACCGGC               [GA]^CCGG[CT]
Eco81I        867  CCTCAGG              CC^T[ACGT]AGG
EcoRI        1268  GAATTC               G^AATTC
EcoRV        1650  GATATC               GAT^ATC
Hin1I         839  GACGCC               G[GA]^CG[CT]C
MfeI         1896  CAATTG               C^AATTG
MflI         1352  AGATCC               [GA]^GATC[CT]
MseI          624  TTAA                 T^TAA
MunI         1896  CAATTG               C^AATTG
NheI          386  GCTAGC               G^CTAGC
PflFI        1700  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PflMI         158  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI         674  CACGTG               CAC^GTG
PmlI          674  CACGTG               CAC^GTG
SapI          453  GCTCTTC              GCTCTTC(1/4)
SmaI          268  CCCGGG               CCC^GGG
SpeI          950  ACTAGT               A^CTAGT
Tru9I         624  TTAA                 T^TAA
Tth111I      1700  GACAGGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
Van91I        158  CCAGTTCCTGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII        1352  AGATCC               [GA]^GATC[CT]
XmaCI         268  CCCGGG               C^CCGGG
XmaI          268  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   24 enzymes) #
###################################

AcsI          253  AAATTC               [GA]^AATT[CT]
             1268  GAATTC              
Alw44I        329  GTGCAC               G^TGCAC
             1073  GTGCAC              
AlwI         1353  GATCC                GGATC(4/5)
             1431  GATCC               
ApaLI         329  GTGCAC               G^TGCAC
             1073  GTGCAC              
ApoI          253  AAATTC               [GA]^AATT[CT]
             1268  GAATTC              
AvaI           45  CCCGAG               C^[CT]CG[GA]G
              268  CCCGGG              
BbsI         1857  GAAGAC               GAAGAC(2/6)
              284  GTCTTC              
BglI          877  GCCACCAGGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
             1483  GCCTCCAGGGC         
BlpI          412  GCTCAGC              GC^T[ACGT]AGC
              582  GCTGAGC             
Bpu1102I      412  GCTCAGC              GC^T[ACGT]AGC
              582  GCTGAGC             
BpuAI        1857  GAAGAC               GAAGAC(2/6)
              284  GTCTTC              
BsaAI         571  TACGTG               [CT]AC^GT[GA]
              674  CACGTG              
BsmBI        1522  CGTCTC               CGTCTC(1/5)
             1497  GAGACG              
BsmFI         140  GGGAC                GGGAC(10/14)
             1122  GGGAC               
BsoBI          45  CCCGAG               C^[CT]CG[GA]G
              268  CCCGGG              
CelII         412  GCTCAGC              GC^T[ACGT]AGC
              582  GCTGAGC             
DraII          76  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1168  GGGTCCT             
EcoO109I       76  AGGACCC              [GA]G^G[ACGT]CC[CT]
             1168  GGGTCCT             
HgaI          839  GACGC                GACGC(5/10)
              404  GCGTC               
HincII        946  GTCAAC               GT[CT]^[GA]AC
             1472  GTCAAC              
HindII        946  GTCAAC               GT[CT]^[GA]AC
             1472  GTCAAC              
MspA1I        580  CAGCTG               C[AC]G^C[GT]G
             1146  CAGCTG              
PpuMI          76  AGGACCC              [GA]G^G[AT]CC[CT]
             1168  GGGTCCT             
PvuII         580  CAGCTG               CAG^CTG
             1146  CAGCTG              


#########################################
# 3 OR MORE CUT ENZYMES (   79 enzymes) #
#########################################

AciI        AfaI        AluI        AlwNI       AspHI       
AvaII       BalI        BanII       BbvI        BcnI        
BfaI        BmyI        BpmI        BsaJI       BseRI       
BsiHKAI     BsiYI       BslI        BsmAI       Bsp1286I    
BspMI       BsrI        BssKI       BstF5I      BstNI       
BstXI       Cac8I       Cfr13I      DdeI        DpnII       
DsaI        EaeI        EarI        EcoRII      EcoT14I     
Fnu4HI      FokI        HaeIII      HapII       HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeI        
MaeII       MaeIII      MboI        MboII       MluNI       
MnlI        MscI        MslI        MspI        MvaI        
MwoI        NciI        NcoI        NdeII       NlaIII      
NlaIV       PleI        PstI        RsaI        SacI        
Sau3AI      Sau96I      ScrFI       SfaNI       SfcI        
SmlI        StyI        TaqI        TfiI        TseI        
Tsp45I      Tsp509I     TspRI       TthHB8I