##################################
# NO CUT ENZYMES ( 114 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AgeI AhdI Aor51HI AscI
AseI AsnI Asp700I Asp718I AspEI
AviII AvrII BamHI BanI BbeI
BclI BglII BlnI BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII Bst1107I
BstBI BstEII BstPI BstUI BstZ17I
CfoI ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoT22I FauI
FbaI FseI FspI HaeII HhaI
HinP1I HindIII HpaI KasI KpnI
KspI MamI MluI MroI MvnI
NaeI NarI NdeI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmeI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SnaBI SphI
Sse8387I SspBI SspI StuI SwaI
XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AcyI 839 GACGCC G[GA]^CG[CT]C
AflII 623 CTTAAG C^TTAAG
AflIII 572 ACGTGT A^C[GA][CT]GT
AocI 867 CCTCAGG CC^T[ACGT]AGG
ApaI 651 GGGCCC GGGCC^C
AspI 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbrPI 674 CACGTG CAC^GTG
BbvCI 1102 CCTCAGC CCTCAGC(-5/-2)
BcgI 74 CGAGGACCCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1546 GTATCC GTATCC(6/5)
BfrI 623 CTTAAG C^TTAAG
BsaHI 839 GACGCC G[GA]^CG[CT]C
BsgI 1134 CTGCAC GTGCAG(16/14)
BsrFI 143 ACCGGC [GA]^CCGG[CT]
BssSI 399 CACGAG CACGAG(-5/-1)
BstAPI 1136 GCACCAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 1352 AGATCC [GA]^GATC[CT]
Bsu36I 867 CCTCAGG CC^T[ACGT]AGG
Cfr10I 143 ACCGGC [GA]^CCGG[CT]
Eco81I 867 CCTCAGG CC^T[ACGT]AGG
EcoRI 1268 GAATTC G^AATTC
EcoRV 1650 GATATC GAT^ATC
Hin1I 839 GACGCC G[GA]^CG[CT]C
MfeI 1896 CAATTG C^AATTG
MflI 1352 AGATCC [GA]^GATC[CT]
MseI 624 TTAA T^TAA
MunI 1896 CAATTG C^AATTG
NheI 386 GCTAGC G^CTAGC
PflFI 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 158 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 674 CACGTG CAC^GTG
PmlI 674 CACGTG CAC^GTG
SapI 453 GCTCTTC GCTCTTC(1/4)
SmaI 268 CCCGGG CCC^GGG
SpeI 950 ACTAGT A^CTAGT
Tru9I 624 TTAA T^TAA
Tth111I 1700 GACAGGGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 158 CCAGTTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1352 AGATCC [GA]^GATC[CT]
XmaCI 268 CCCGGG C^CCGGG
XmaI 268 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AcsI 253 AAATTC [GA]^AATT[CT]
1268 GAATTC
Alw44I 329 GTGCAC G^TGCAC
1073 GTGCAC
AlwI 1353 GATCC GGATC(4/5)
1431 GATCC
ApaLI 329 GTGCAC G^TGCAC
1073 GTGCAC
ApoI 253 AAATTC [GA]^AATT[CT]
1268 GAATTC
AvaI 45 CCCGAG C^[CT]CG[GA]G
268 CCCGGG
BbsI 1857 GAAGAC GAAGAC(2/6)
284 GTCTTC
BglI 877 GCCACCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1483 GCCTCCAGGGC
BlpI 412 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
Bpu1102I 412 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
BpuAI 1857 GAAGAC GAAGAC(2/6)
284 GTCTTC
BsaAI 571 TACGTG [CT]AC^GT[GA]
674 CACGTG
BsmBI 1522 CGTCTC CGTCTC(1/5)
1497 GAGACG
BsmFI 140 GGGAC GGGAC(10/14)
1122 GGGAC
BsoBI 45 CCCGAG C^[CT]CG[GA]G
268 CCCGGG
CelII 412 GCTCAGC GC^T[ACGT]AGC
582 GCTGAGC
DraII 76 AGGACCC [GA]G^G[ACGT]CC[CT]
1168 GGGTCCT
EcoO109I 76 AGGACCC [GA]G^G[ACGT]CC[CT]
1168 GGGTCCT
HgaI 839 GACGC GACGC(5/10)
404 GCGTC
HincII 946 GTCAAC GT[CT]^[GA]AC
1472 GTCAAC
HindII 946 GTCAAC GT[CT]^[GA]AC
1472 GTCAAC
MspA1I 580 CAGCTG C[AC]G^C[GT]G
1146 CAGCTG
PpuMI 76 AGGACCC [GA]G^G[AT]CC[CT]
1168 GGGTCCT
PvuII 580 CAGCTG CAG^CTG
1146 CAGCTG
#########################################
# 3 OR MORE CUT ENZYMES ( 79 enzymes) #
#########################################
AciI AfaI AluI AlwNI AspHI
AvaII BalI BanII BbvI BcnI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DsaI EaeI EarI EcoRII EcoT14I
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MluNI
MnlI MscI MslI MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I