##################################
# NO CUT ENZYMES ( 87 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
Alw44I ApaI ApaLI AscI AseI
AsnI Asp718I AspI AviII AvrII
BamHI BbrPI BbvCI BcgI BciVI
BclI BfrI BlnI BsaAI BsaBI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BsrGI BssHII Bst1107I BstBI BstZ17I
ClaI CpoI DraI DrdI EagI
EarI EclXI Eco52I EcoRI EcoRV
EcoT22I FbaI FseI FspI HindIII
KpnI Ksp632I MamI MfeI MluI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SalI SapI SfiI SfuI SgrAI
SnaBI SpeI SspBI SspI StuI
SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 2334 GTAGAC GT^[AC][GT]AC
AcsI 1710 GAATTT [GA]^AATT[CT]
AflIII 1844 ACATGT A^C[GA][CT]GT
AhdI 57 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1893 GATCC GGATC(4/5)
Aor51HI 1186 AGCGCT AGC^GCT
ApoI 1710 GAATTT [GA]^AATT[CT]
AspEI 57 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BglI 728 GCCCACTGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1314 AGATCT A^GATCT
BlpI 1522 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1522 GCTTAGC GC^T[ACGT]AGC
BsgI 368 CTGCAC GTGCAG(16/14)
BsmI 1885 GCATTC GAATGC(1/-1)
BspHI 826 TCATGA T^CATGA
BspLU11I 1844 ACATGT A^CATGT
BsrBI 1478 GAGCGG CCGCTC(-3/-3)
BsrFI 1458 ACCGGC [GA]^CCGG[CT]
BssSI 1436 CACGAG CACGAG(-5/-1)
BstEII 1973 GGTGACC G^GT[ACGT]ACC
BstPI 1973 GGTGACC G^GT[ACGT]ACC
BstXI 1495 CCACATCTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1522 GCTTAGC GC^T[ACGT]AGC
Cfr10I 1458 ACCGGC [GA]^CCGG[CT]
Eam1105I 57 GACCTTCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1186 AGCGCT AGC^GCT
EcoO65I 1973 GGTGACC G^GT[ACGT]ACC
HincII 2068 GTTAAC GT[CT]^[GA]AC
HindII 2068 GTTAAC GT[CT]^[GA]AC
HpaI 2068 GTTAAC GTT^AAC
KspI 654 CCGCGG CCGC^GG
PaeR7I 1356 CTCGAG C^TCGAG
PvuII 625 CAGCTG CAG^CTG
RcaI 826 TCATGA T^CATGA
SacII 654 CCGCGG CCGC^GG
SbfI 832 CCTGCAGG CCTGCA^GG
ScaI 2369 AGTACT AGT^ACT
SmaI 1000 CCCGGG CCC^GGG
SphI 680 GCATGC GCATG^C
Sse8387I 832 CCTGCAGG CCTGCA^GG
TfiI 345 GATTC G^A[AT]TC
XbaI 1902 TCTAGA T^CTAGA
XhoI 1356 CTCGAG C^TCGAG
XmaCI 1000 CCCGGG C^CCGGG
XmaI 1000 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 39 enzymes) #
###################################
AccII 655 CGCG CG^CG
782 CGCG
AccIII 53 TCCGGA T^CCGGA
883 TCCGGA
AlwNI 639 CAGAGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1965 CAGCCACTG
AocI 196 CCTGAGG CC^T[ACGT]AGG
338 CCTAAGG
Asp700I 754 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1339 GAAGCTGTTC
BbeI 18 GGCGCC GGCGC^C
201 GGCGCC
BbsI 71 GAAGAC GAAGAC(2/6)
1061 GAAGAC
BpuAI 71 GAAGAC GAAGAC(2/6)
1061 GAAGAC
BsaWI 53 TCCGGA [AT]^CCGG[AT]
883 TCCGGA
BseAI 53 TCCGGA T^CCGGA
883 TCCGGA
BspEI 53 TCCGGA T^CCGGA
883 TCCGGA
BsrDI 993 CATTGC GCAATG(2/0)
843 GCAATG
BstF5I 1670 CATCC GGATG(2/0)
2132 CATCC
BstUI 655 CGCG CG^CG
782 CGCG
BstYI 1314 AGATCT [GA]^GATC[CT]
1892 AGATCC
Bsu36I 196 CCTGAGG CC^T[ACGT]AGG
338 CCTAAGG
DraIII 374 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
1871 CACCCTGTG
Eco81I 196 CCTGAGG CC^T[ACGT]AGG
338 CCTAAGG
EcoNI 690 CCTGTGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2321 CCTGGTGGAGG
FokI 1670 CATCC GGATG(9/13)
2132 CATCC
HgaI 941 GACGC GACGC(5/10)
1453 GCGTC
KasI 18 GGCGCC G^GCGCC
201 GGCGCC
MaeII 552 ACGT A^CGT
2413 ACGT
MflI 1314 AGATCT [GA]^GATC[CT]
1892 AGATCC
MroI 53 TCCGGA T^CCGGA
883 TCCGGA
MseI 1866 TTAA T^TAA
2069 TTAA
MspA1I 625 CAGCTG C[AC]G^C[GT]G
654 CCGCGG
MvnI 655 CGCG CG^CG
782 CGCG
NarI 18 GGCGCC GG^CGCC
201 GGCGCC
NcoI 709 CCATGG C^CATGG
1222 CCATGG
PflMI 373 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1964 CCAGCCACTGG
SacI 422 GAGCTC GAGCT^C
526 GAGCTC
SexAI 375 ACCAGGT A^CC[AT]GGT
2320 ACCTGGT
SfoI 18 GGCGCC GGC^GCC
201 GGCGCC
Tru9I 1866 TTAA T^TAA
2069 TTAA
Van91I 373 CCACCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1964 CCAGCCACTGG
XcmI 624 CCAGCTGGGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
854 CCAACGCAGGTTTGG
XhoII 1314 AGATCT [GA]^GATC[CT]
1892 AGATCC
XmnI 754 GAAGCCATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1339 GAAGCTGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 87 enzymes) #
#########################################
AciI AcyI AfaI AluI AspHI
AvaI AvaII BalI BanI BanII
BbvI BcnI BfaI BmyI BpmI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstAPI
BstNI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EcoO109I
EcoRII EcoT14I FauI Fnu4HI HaeII
HaeIII HapII HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PpuMI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TseI Tsp45I Tsp509I
TspRI TthHB8I