##################################
# NO CUT ENZYMES ( 113 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AgeI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspI AviII
AvrII BalI BbeI BbrPI BcgI
BciVI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsmBI Bsp1407I BspDI BspEI BspHI
BsrBI BsrGI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI EaeI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I KasI KpnI KspI
MamI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XbaI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccI 1279 GTAGAC GT^[AC][GT]AC
AccII 818 CGCG CG^CG
AhdI 2375 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1914 GTGCAC G^TGCAC
AlwNI 36 CAGTGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1914 GTGCAC G^TGCAC
AspEI 2375 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 245 GGATCC G^GATCC
BanI 1057 GGTGCC G^G[CT][GA]CC
BbsI 1252 GTCTTC GAAGAC(2/6)
BglI 73 GCCTCAGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1252 GTCTTC GAAGAC(2/6)
BsaI 1073 GGTCTC GGTCTC(1/5)
BsiEI 284 CGATCG CG[GA][CT]^CG
BsiWI 1630 CGTACG C^GTACG
BsmI 2118 GAATGC GAATGC(1/-1)
BspMI 1310 ACCTGC ACCTGC(4/8)
BsrDI 1067 GCAATG GCAATG(2/0)
BsrFI 91 ACCGGC [GA]^CCGG[CT]
BstUI 818 CGCG CG^CG
BstXI 1497 CCAGGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 91 ACCGGC [GA]^CCGG[CT]
DraII 455 AGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 586 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1584 GACGGATCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 572 CCATGG C^C[GA][CT]GG
Eam1105I 2375 GACAACATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 455 AGGGCCT [GA]G^G[ACGT]CC[CT]
EcoT22I 2120 ATGCAT ATGCA^T
FauI 912 GCGGG CCCGC(4/6)
HindIII 804 AAGCTT A^AGCTT
HpaI 724 GTTAAC GTT^AAC
MfeI 1229 CAATTG C^AATTG
MslI 390 CATCTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 580 CAGCTG C[AC]G^C[GT]G
MunI 1229 CAATTG C^AATTG
MvnI 818 CGCG CG^CG
NcoI 572 CCATGG C^CATGG
NdeI 2048 CATATG CA^TATG
NsiI 2120 ATGCAT ATGCA^T
PaeR7I 712 CTCGAG C^TCGAG
PshAI 2400 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 64 CTGCAG CTGCA^G
PvuI 284 CGATCG CGAT^CG
PvuII 580 CAGCTG CAG^CTG
SacI 1207 GAGCTC GAGCT^C
SmaI 10 CCCGGG CCC^GGG
SpeI 219 ACTAGT A^CTAGT
StuI 871 AGGCCT AGG^CCT
XhoI 712 CTCGAG C^TCGAG
XmaCI 10 CCCGGG C^CCGGG
XmaI 10 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AflII 1834 CTTAAG C^TTAAG
2207 CTTAAG
AflIII 176 ACATGT A^C[GA][CT]GT
2379 ACATGT
AvaI 10 CCCGGG C^[CT]CG[GA]G
712 CTCGAG
AvaII 576 GGACC G^G[AT]CC
1399 GGACC
BbvCI 522 CCTCAGC CCTCAGC(-5/-2)
2030 CCTCAGC
BclI 224 TGATCA T^GATCA
633 TGATCA
BcnI 10 CCCGG CC^[GC]GG
11 CCGGG
BfaI 220 CTAG C^TAG
1480 CTAG
BfrI 1834 CTTAAG C^TTAAG
2207 CTTAAG
BsmFI 1426 GGGAC GGGAC(10/14)
2103 GTCCC
BsoBI 10 CCCGGG C^[CT]CG[GA]G
712 CTCGAG
BspLU11I 176 ACATGT A^CATGT
2379 ACATGT
FbaI 224 TGATCA T^GATCA
633 TGATCA
HapII 11 CCGG C^CGG
92 CCGG
HincII 724 GTTAAC GT[CT]^[GA]AC
1979 GTTGAC
HindII 724 GTTAAC GT[CT]^[GA]AC
1979 GTTGAC
HpaII 11 CCGG C^CGG
92 CCGG
MaeI 220 CTAG C^TAG
1480 CTAG
MspI 11 CCGG C^CGG
92 CCGG
NciI 10 CCCGG CC^[GC]GG
11 CCGGG
XcmI 149 CCACCCTGGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
679 CCAACCTTTGTGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 72 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I AspHI BanII BbvI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRI EcoRII EcoT14I Fnu4HI FokI
HaeIII HinfI HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MseI MvaI MwoI NdeII
NlaIII NlaIV NspI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmnI