##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
Asp718I AspEI AspI AviII AvrII
BanI BbeI BbvCI BcgI BciVI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BsiWI Bsp1407I BspDI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstXI BstZ17I CelII
Cfr10I ClaI CpoI DraI DrdI
DsaI Eam1105I EcoRV EcoT22I FseI
FspI HincII HindII HindIII HpaI
KasI KpnI KspI MamI MfeI
MluI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmeI PpuMI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tth111I Van91I XcmI XhoI
###################################
# ONE CUT ENZYMES ( 54 enzymes) #
###################################
AccI 1721 GTCTAC GT^[AC][GT]AC
AccII 155 CGCG CG^CG
AcyI 2243 GGCGTC G[GA]^CG[CT]C
Alw44I 787 GTGCAC G^TGCAC
AocI 740 CCTGAGG CC^T[ACGT]AGG
Aor51HI 1615 AGCGCT AGC^GCT
ApaLI 787 GTGCAC G^TGCAC
AvaII 1073 GGACC G^G[AT]CC
BalI 2250 TGGCCA TGG^CCA
BamHI 215 GGATCC G^GATCC
BbrPI 705 CACGTG CAC^GTG
BbsI 318 GAAGAC GAAGAC(2/6)
BclI 620 TGATCA T^GATCA
BpuAI 318 GAAGAC GAAGAC(2/6)
BsaHI 2243 GGCGTC G[GA]^CG[CT]C
BsaI 1695 GAGACC GGTCTC(1/5)
BsiEI 78 CGGCCG CG[GA][CT]^CG
BsmBI 5 GAGACG CGTCTC(1/5)
BsmI 867 GAATGC GAATGC(1/-1)
BspMI 823 ACCTGC ACCTGC(4/8)
BstEII 122 GGTTACC G^GT[ACGT]ACC
BstPI 122 GGTTACC G^GT[ACGT]ACC
BstUI 155 CGCG CG^CG
Bsu36I 740 CCTGAGG CC^T[ACGT]AGG
DraII 942 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 790 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 78 CGGCCG C^GGCCG
EarI 1608 GAAGAG CTCTTC(1/4)
EclXI 78 CGGCCG C^GGCCG
Eco47III 1615 AGCGCT AGC^GCT
Eco52I 78 CGGCCG C^GGCCG
Eco81I 740 CCTGAGG CC^T[ACGT]AGG
EcoNI 1397 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 942 AGGCCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 122 GGTTACC G^GT[ACGT]ACC
EcoRI 925 GAATTC G^AATTC
EcoT14I 1883 CCAAGG C^C[AT][AT]GG
FbaI 620 TGATCA T^GATCA
HaeII 1615 AGCGCT [GA]GCGC^[CT]
HgaI 2244 GCGTC GACGC(5/10)
Hin1I 2243 GGCGTC G[GA]^CG[CT]C
Ksp632I 1608 GAAGAG CTCTTC(1/4)
MluNI 2250 TGGCCA TGG^CCA
MscI 2250 TGGCCA TGG^CCA
MvnI 155 CGCG CG^CG
NspI 755 GCATGT [GA]CATG^[CT]
PmaCI 705 CACGTG CAC^GTG
PmlI 705 CACGTG CAC^GTG
PstI 1398 CTGCAG CTGCA^G
ScaI 1990 AGTACT AGT^ACT
SmaI 117 CCCGGG CCC^GGG
StyI 1883 CCAAGG C^C[AT][AT]GG
XmaCI 117 CCCGGG C^CCGGG
XmaI 117 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 11 enzymes) #
###################################
AccIII 179 TCCGGA T^CCGGA
218 TCCGGA
BsaWI 179 TCCGGA [AT]^CCGG[AT]
218 TCCGGA
BseAI 179 TCCGGA T^CCGGA
218 TCCGGA
BseRI 374 CTCCTC GAGGAG(10/8)
654 CTCCTC
BspEI 179 TCCGGA T^CCGGA
218 TCCGGA
BspHI 314 TCATGA T^CATGA
2350 TCATGA
MroI 179 TCCGGA T^CCGGA
218 TCCGGA
PvuII 333 CAGCTG CAG^CTG
1910 CAGCTG
RcaI 314 TCATGA T^CATGA
2350 TCATGA
StuI 858 AGGCCT AGG^CCT
1138 AGGCCT
XbaI 1944 TCTAGA T^CTAGA
1956 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApoI Asp700I AspHI
AvaI BanII BbvI BcnI BfaI
BglII BmyI BpmI BsaAI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrBI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I XhoII XmnI