################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI ApaI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BanI BbeI BbvCI BcgI BciVI BfrI BglI BlnI BlpI Bpu1102I BsaBI BsiWI Bsp1407I BspDI BspLU11I BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstBI BstXI BstZ17I CelII Cfr10I ClaI CpoI DraI DrdI DsaI Eam1105I EcoRV EcoT22I FseI FspI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI NaeI NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PflMI PinAI PmeI PpuMI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SspI SwaI Tth111I Van91I XcmI XhoI ################################### # ONE CUT ENZYMES ( 54 enzymes) # ################################### AccI 1721 GTCTAC GT^[AC][GT]AC AccII 155 CGCG CG^CG AcyI 2243 GGCGTC G[GA]^CG[CT]C Alw44I 787 GTGCAC G^TGCAC AocI 740 CCTGAGG CC^T[ACGT]AGG Aor51HI 1615 AGCGCT AGC^GCT ApaLI 787 GTGCAC G^TGCAC AvaII 1073 GGACC G^G[AT]CC BalI 2250 TGGCCA TGG^CCA BamHI 215 GGATCC G^GATCC BbrPI 705 CACGTG CAC^GTG BbsI 318 GAAGAC GAAGAC(2/6) BclI 620 TGATCA T^GATCA BpuAI 318 GAAGAC GAAGAC(2/6) BsaHI 2243 GGCGTC G[GA]^CG[CT]C BsaI 1695 GAGACC GGTCTC(1/5) BsiEI 78 CGGCCG CG[GA][CT]^CG BsmBI 5 GAGACG CGTCTC(1/5) BsmI 867 GAATGC GAATGC(1/-1) BspMI 823 ACCTGC ACCTGC(4/8) BstEII 122 GGTTACC G^GT[ACGT]ACC BstPI 122 GGTTACC G^GT[ACGT]ACC BstUI 155 CGCG CG^CG Bsu36I 740 CCTGAGG CC^T[ACGT]AGG DraII 942 AGGCCCT [GA]G^G[ACGT]CC[CT] DraIII 790 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG EagI 78 CGGCCG C^GGCCG EarI 1608 GAAGAG CTCTTC(1/4) EclXI 78 CGGCCG C^GGCCG Eco47III 1615 AGCGCT AGC^GCT Eco52I 78 CGGCCG C^GGCCG Eco81I 740 CCTGAGG CC^T[ACGT]AGG EcoNI 1397 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO109I 942 AGGCCCT [GA]G^G[ACGT]CC[CT] EcoO65I 122 GGTTACC G^GT[ACGT]ACC EcoRI 925 GAATTC G^AATTC EcoT14I 1883 CCAAGG C^C[AT][AT]GG FbaI 620 TGATCA T^GATCA HaeII 1615 AGCGCT [GA]GCGC^[CT] HgaI 2244 GCGTC GACGC(5/10) Hin1I 2243 GGCGTC G[GA]^CG[CT]C Ksp632I 1608 GAAGAG CTCTTC(1/4) MluNI 2250 TGGCCA TGG^CCA MscI 2250 TGGCCA TGG^CCA MvnI 155 CGCG CG^CG NspI 755 GCATGT [GA]CATG^[CT] PmaCI 705 CACGTG CAC^GTG PmlI 705 CACGTG CAC^GTG PstI 1398 CTGCAG CTGCA^G ScaI 1990 AGTACT AGT^ACT SmaI 117 CCCGGG CCC^GGG StyI 1883 CCAAGG C^C[AT][AT]GG XmaCI 117 CCCGGG C^CCGGG XmaI 117 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 11 enzymes) # ################################### AccIII 179 TCCGGA T^CCGGA 218 TCCGGA BsaWI 179 TCCGGA [AT]^CCGG[AT] 218 TCCGGA BseAI 179 TCCGGA T^CCGGA 218 TCCGGA BseRI 374 CTCCTC GAGGAG(10/8) 654 CTCCTC BspEI 179 TCCGGA T^CCGGA 218 TCCGGA BspHI 314 TCATGA T^CATGA 2350 TCATGA MroI 179 TCCGGA T^CCGGA 218 TCCGGA PvuII 333 CAGCTG CAG^CTG 1910 CAGCTG RcaI 314 TCATGA T^CATGA 2350 TCATGA StuI 858 AGGCCT AGG^CCT 1138 AGGCCT XbaI 1944 TCTAGA T^CTAGA 1956 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 89 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApoI Asp700I AspHI AvaI BanII BbvI BcnI BfaI BglII BmyI BpmI BsaAI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrBI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII XmnI