##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AatII Acc65I AccI AclI AflII
AgeI AscI AseI AsnI Asp700I
Asp718I AviII BbrPI BcgI BciVI
BclI BfrI BglII BsaAI BsaBI
BspDI BspHI BspLU11I BsrBI BssSI
Bst1107I BstAPI BstBI BstZ17I ClaI
CpoI DraIII DrdI EcoRI EcoRV
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI KpnI KspI
MamI MfeI MluI MunI NcoI
NdeI NheI NotI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SacII SalI SfiI SfuI
SnaBI SpeI SspI SwaI XbaI
XmnI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccIII 1121 TCCGGA T^CCGGA
AflIII 780 ACGTGT A^C[GA][CT]GT
AlwNI 184 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 1237 AGCGCT AGC^GCT
ApaI 426 GGGCCC GGGCC^C
AvrII 1677 CCTAGG C^CTAGG
BalI 774 TGGCCA TGG^CCA
BamHI 2246 GGATCC G^GATCC
BbvCI 1072 GCTGAGG CCTCAGC(-5/-2)
BglI 127 GCCAGCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1677 CCTAGG C^CTAGG
BlpI 1624 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1624 GCTTAGC GC^T[ACGT]AGC
BsaI 951 GAGACC GGTCTC(1/5)
BsaWI 1121 TCCGGA [AT]^CCGG[AT]
BseAI 1121 TCCGGA T^CCGGA
BsgI 1660 CTGCAC GTGCAG(16/14)
BsiEI 113 CGGCCG CG[GA][CT]^CG
BsmBI 702 GAGACG CGTCTC(1/5)
BsmI 1823 GCATTC GAATGC(1/-1)
Bsp1407I 2295 TGTACA T^GTACA
BspEI 1121 TCCGGA T^CCGGA
BspMI 719 ACCTGC ACCTGC(4/8)
BsrDI 1155 CATTGC GCAATG(2/0)
BsrFI 37 GCCGGC [GA]^CCGG[CT]
BsrGI 2295 TGTACA T^GTACA
BssHII 18 GCGCGC G^CGCGC
BstXI 996 CCACGGTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 1624 GCTTAGC GC^T[ACGT]AGC
Cfr10I 37 GCCGGC [GA]^CCGG[CT]
DraI 2049 TTTAAA TTT^AAA
EagI 113 CGGCCG C^GGCCG
EclXI 113 CGGCCG C^GGCCG
Eco47III 1237 AGCGCT AGC^GCT
Eco52I 113 CGGCCG C^GGCCG
MluNI 774 TGGCCA TGG^CCA
MroI 1121 TCCGGA T^CCGGA
MscI 774 TGGCCA TGG^CCA
NaeI 37 GCCGGC GCC^GGC
NgoMIV 37 GCCGGC G^CCGGC
NruI 887 TCGCGA TCG^CGA
NspI 1647 GCATGC [GA]CATG^[CT]
PaeR7I 2109 CTCGAG C^TCGAG
PflMI 777 CCAACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 716 AGGACCT [GA]G^G[AT]CC[CT]
PvuII 1804 CAGCTG CAG^CTG
SacI 2069 GAGCTC GAGCT^C
SapI 345 GAAGAGC GCTCTTC(1/4)
SbfI 180 CCTGCAGG CCTGCA^GG
ScaI 1874 AGTACT AGT^ACT
SexAI 1115 ACCTGGT A^CC[AT]GGT
SgrAI 36 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 2109 CTCGAG C^T[CT][GA]AG
SphI 1647 GCATGC GCATG^C
Sse8387I 180 CCTGCAGG CCTGCA^GG
SspBI 2295 TGTACA T^GTACA
TfiI 2183 GATTC G^A[AT]TC
Van91I 777 CCAACGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 2109 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 2059 AAATTC [GA]^AATT[CT]
2226 AAATTT
AhdI 516 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACCAGGAGTC
Alw44I 1333 GTGCAC G^TGCAC
2023 GTGCAC
AocI 1587 CCTTAGG CC^T[ACGT]AGG
1833 CCTGAGG
ApaLI 1333 GTGCAC G^TGCAC
2023 GTGCAC
ApoI 2059 AAATTC [GA]^AATT[CT]
2226 AAATTT
AspEI 516 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACCAGGAGTC
BpmI 381 CTCCAG CTGGAG(16/14)
640 CTCCAG
BsiWI 572 CGTACG C^GTACG
755 CGTACG
BsmFI 173 GGGAC GGGAC(10/14)
168 GTCCC
BstEII 231 GGTCACC G^GT[ACGT]ACC
273 GGTGACC
BstPI 231 GGTCACC G^GT[ACGT]ACC
273 GGTGACC
Bsu36I 1587 CCTTAGG CC^T[ACGT]AGG
1833 CCTGAGG
Eam1105I 516 GACGAAAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1435 GACCAGGAGTC
Eco81I 1587 CCTTAGG CC^T[ACGT]AGG
1833 CCTGAGG
EcoO65I 231 GGTCACC G^GT[ACGT]ACC
273 GGTGACC
FauI 841 CCCGC CCCGC(4/6)
286 GCGGG
MaeII 780 ACGT A^CGT
1793 ACGT
TaqI 924 TCGA T^CGA
2110 TCGA
TthHB8I 924 TCGA T^CGA
2110 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AspI AvaI AvaII
BanI BanII BbeI BbsI BbvI
BcnI BfaI BmyI BpuAI BsaHI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
KasI Ksp632I MaeI MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NarI NciI NdeII NlaIII NlaIV
PflFI PleI PstI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmaI StuI StyI Tru9I TseI
Tsp45I Tsp509I TspRI Tth111I XcmI
XhoII XmaCI XmaI