################################## # NO CUT ENZYMES ( 101 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI AhdI AocI AscI AseI AsnI AspEI AspI AviII BbrPI BcgI BfrI BglI BsaAI BsaBI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI BssHII BssSI BstBI Bsu36I ClaI CpoI DraI DraIII DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoRI EcoRV EcoT22I FseI FspI HpaI KspI MamI MfeI MluI MroI MseI MunI NcoI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1448 GGTACC G^GTACC AcsI 1962 GAATTT [GA]^AATT[CT] Alw44I 1382 GTGCAC G^TGCAC AlwNI 1280 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG Aor51HI 981 AGCGCT AGC^GCT ApaLI 1382 GTGCAC G^TGCAC ApoI 1962 GAATTT [GA]^AATT[CT] Asp700I 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1448 GGTACC G^GTACC AvaI 2162 CTCGGG C^[CT]CG[GA]G AvrII 1348 CCTAGG C^CTAGG BamHI 2157 GGATCC G^GATCC BciVI 1366 GGATAC GTATCC(6/5) BclI 1472 TGATCA T^GATCA BglII 1978 AGATCT A^GATCT BlnI 1348 CCTAGG C^CTAGG BsaI 2167 GAGACC GGTCTC(1/5) BsoBI 2162 CTCGGG C^[CT]CG[GA]G BspMI 174 ACCTGC ACCTGC(4/8) BsrBI 1024 CCGCTC CCGCTC(-3/-3) Bst1107I 1322 GTATAC GTA^TAC BstEII 1503 GGTCACC G^GT[ACGT]ACC BstPI 1503 GGTCACC G^GT[ACGT]ACC BstZ17I 1322 GTATAC GTA^TAC Eco47III 981 AGCGCT AGC^GCT EcoNI 224 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 1503 GGTCACC G^GT[ACGT]ACC FbaI 1472 TGATCA T^GATCA HgaI 824 GACGC GACGC(5/10) HincII 2252 GTCAAC GT[CT]^[GA]AC HindII 2252 GTCAAC GT[CT]^[GA]AC KpnI 1448 GGTACC GGTAC^C MslI 313 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG PshAI 2146 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC PstI 176 CTGCAG CTGCA^G SacI 1267 GAGCTC GAGCT^C SfcI 176 CTGCAG C^T[GA][CT]AG SmlI 2141 CTTGAG C^T[CT][GA]AG TaqI 139 TCGA T^CGA TthHB8I 139 TCGA T^CGA XbaI 433 TCTAGA T^CTAGA XcmI 171 CCAACCTGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmnI 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 1322 GTATAC GT^[AC][GT]AC 1885 GTCTAC AccII 329 CGCG CG^CG 1220 CGCG ApaI 1011 GGGCCC GGGCC^C 1493 GGGCCC AspHI 1267 GAGCTC G[AT]GC[AT]^C 1382 GTGCAC BalI 183 TGGCCA TGG^CCA 1956 TGGCCA BbeI 579 GGCGCC GGCGC^C 1344 GGCGCC BbsI 56 GAAGAC GAAGAC(2/6) 2057 GTCTTC BbvCI 1199 CCTCAGC CCTCAGC(-5/-2) 2246 GCTGAGG BcnI 586 CCGGG CC^[GC]GG 1432 CCGGG BpuAI 56 GAAGAC GAAGAC(2/6) 2057 GTCTTC BsgI 380 GTGCAG GTGCAG(16/14) 443 GTGCAG BsiHKAI 1267 GAGCTC G[AT]GC[AT]^C 1382 GTGCAC BsrFI 1032 GCCGGC [GA]^CCGG[CT] 1185 GCCGGC BstAPI 1076 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1279 GCAGACACTGC BstUI 329 CGCG CG^CG 1220 CGCG BstYI 1978 AGATCT [GA]^GATC[CT] 2157 GGATCC Cfr10I 1032 GCCGGC [GA]^CCGG[CT] 1185 GCCGGC HindIII 1817 AAGCTT A^AGCTT 1917 AAGCTT KasI 579 GGCGCC G^GCGCC 1344 GGCGCC MaeII 613 ACGT A^CGT 952 ACGT MflI 1978 AGATCT [GA]^GATC[CT] 2157 GGATCC MluNI 183 TGGCCA TGG^CCA 1956 TGGCCA MscI 183 TGGCCA TGG^CCA 1956 TGGCCA MvnI 329 CGCG CG^CG 1220 CGCG NaeI 1032 GCCGGC GCC^GGC 1185 GCCGGC NarI 579 GGCGCC GG^CGCC 1344 GGCGCC NciI 586 CCGGG CC^[GC]GG 1432 CCGGG NgoMIV 1032 GCCGGC G^CCGGC 1185 GCCGGC NspI 409 GCATGT [GA]CATG^[CT] 849 GCATGT PpuMI 1130 GGGTCCT [GA]G^G[AT]CC[CT] 1700 GGGTCCC SfoI 579 GGCGCC GGC^GCC 1344 GGCGCC Tsp45I 476 GTGAC ^GT[GC]AC 1504 GTCAC XhoII 1978 AGATCT [GA]^GATC[CT] 2157 GGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 81 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AvaII BanI BanII BbvI BfaI BlpI BmyI BpmI Bpu1102I BsaHI BsaJI BseRI BsiYI BslI BsmAI BsmFI Bsp1286I BsrI BssKI BstF5I BstNI BstXI Cac8I CelII CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI Hin1I HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MnlI MspA1I MspI MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PvuII RsaI SapI Sau3AI Sau96I ScrFI SfaNI StyI TfiI TseI Tsp509I TspRI Van91I