##################################
# NO CUT ENZYMES ( 101 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI AhdI AocI AscI AseI
AsnI AspEI AspI AviII BbrPI
BcgI BfrI BglI BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII BssSI
BstBI Bsu36I ClaI CpoI DraI
DraIII DrdI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HpaI KspI MamI
MfeI MluI MroI MseI MunI
NcoI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SbfI ScaI SexAI SfiI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tru9I Tth111I XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1448 GGTACC G^GTACC
AcsI 1962 GAATTT [GA]^AATT[CT]
Alw44I 1382 GTGCAC G^TGCAC
AlwNI 1280 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 981 AGCGCT AGC^GCT
ApaLI 1382 GTGCAC G^TGCAC
ApoI 1962 GAATTT [GA]^AATT[CT]
Asp700I 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1448 GGTACC G^GTACC
AvaI 2162 CTCGGG C^[CT]CG[GA]G
AvrII 1348 CCTAGG C^CTAGG
BamHI 2157 GGATCC G^GATCC
BciVI 1366 GGATAC GTATCC(6/5)
BclI 1472 TGATCA T^GATCA
BglII 1978 AGATCT A^GATCT
BlnI 1348 CCTAGG C^CTAGG
BsaI 2167 GAGACC GGTCTC(1/5)
BsoBI 2162 CTCGGG C^[CT]CG[GA]G
BspMI 174 ACCTGC ACCTGC(4/8)
BsrBI 1024 CCGCTC CCGCTC(-3/-3)
Bst1107I 1322 GTATAC GTA^TAC
BstEII 1503 GGTCACC G^GT[ACGT]ACC
BstPI 1503 GGTCACC G^GT[ACGT]ACC
BstZ17I 1322 GTATAC GTA^TAC
Eco47III 981 AGCGCT AGC^GCT
EcoNI 224 CCTGGGCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1503 GGTCACC G^GT[ACGT]ACC
FbaI 1472 TGATCA T^GATCA
HgaI 824 GACGC GACGC(5/10)
HincII 2252 GTCAAC GT[CT]^[GA]AC
HindII 2252 GTCAAC GT[CT]^[GA]AC
KpnI 1448 GGTACC GGTAC^C
MslI 313 CATCTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PshAI 2146 GACAGGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 176 CTGCAG CTGCA^G
SacI 1267 GAGCTC GAGCT^C
SfcI 176 CTGCAG C^T[GA][CT]AG
SmlI 2141 CTTGAG C^T[CT][GA]AG
TaqI 139 TCGA T^CGA
TthHB8I 139 TCGA T^CGA
XbaI 433 TCTAGA T^CTAGA
XcmI 171 CCAACCTGCAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 283 GAAGCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 1322 GTATAC GT^[AC][GT]AC
1885 GTCTAC
AccII 329 CGCG CG^CG
1220 CGCG
ApaI 1011 GGGCCC GGGCC^C
1493 GGGCCC
AspHI 1267 GAGCTC G[AT]GC[AT]^C
1382 GTGCAC
BalI 183 TGGCCA TGG^CCA
1956 TGGCCA
BbeI 579 GGCGCC GGCGC^C
1344 GGCGCC
BbsI 56 GAAGAC GAAGAC(2/6)
2057 GTCTTC
BbvCI 1199 CCTCAGC CCTCAGC(-5/-2)
2246 GCTGAGG
BcnI 586 CCGGG CC^[GC]GG
1432 CCGGG
BpuAI 56 GAAGAC GAAGAC(2/6)
2057 GTCTTC
BsgI 380 GTGCAG GTGCAG(16/14)
443 GTGCAG
BsiHKAI 1267 GAGCTC G[AT]GC[AT]^C
1382 GTGCAC
BsrFI 1032 GCCGGC [GA]^CCGG[CT]
1185 GCCGGC
BstAPI 1076 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1279 GCAGACACTGC
BstUI 329 CGCG CG^CG
1220 CGCG
BstYI 1978 AGATCT [GA]^GATC[CT]
2157 GGATCC
Cfr10I 1032 GCCGGC [GA]^CCGG[CT]
1185 GCCGGC
HindIII 1817 AAGCTT A^AGCTT
1917 AAGCTT
KasI 579 GGCGCC G^GCGCC
1344 GGCGCC
MaeII 613 ACGT A^CGT
952 ACGT
MflI 1978 AGATCT [GA]^GATC[CT]
2157 GGATCC
MluNI 183 TGGCCA TGG^CCA
1956 TGGCCA
MscI 183 TGGCCA TGG^CCA
1956 TGGCCA
MvnI 329 CGCG CG^CG
1220 CGCG
NaeI 1032 GCCGGC GCC^GGC
1185 GCCGGC
NarI 579 GGCGCC GG^CGCC
1344 GGCGCC
NciI 586 CCGGG CC^[GC]GG
1432 CCGGG
NgoMIV 1032 GCCGGC G^CCGGC
1185 GCCGGC
NspI 409 GCATGT [GA]CATG^[CT]
849 GCATGT
PpuMI 1130 GGGTCCT [GA]G^G[AT]CC[CT]
1700 GGGTCCC
SfoI 579 GGCGCC GGC^GCC
1344 GGCGCC
Tsp45I 476 GTGAC ^GT[GC]AC
1504 GTCAC
XhoII 1978 AGATCT [GA]^GATC[CT]
2157 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AvaII BanI BanII BbvI BfaI
BlpI BmyI BpmI Bpu1102I BsaHI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I CelII CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MwoI NdeII NlaIII
NlaIV PflMI PleI PvuII RsaI
SapI Sau3AI Sau96I ScrFI SfaNI
StyI TfiI TseI Tsp509I TspRI
Van91I