################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## AccIII AclI AflII AocI Aor51HI AscI AseI AsnI Asp700I AviII BbrPI BbvCI BciVI BclI BfrI BsaBI BseAI BsiEI BsiWI BsmI Bsp1407I BspDI BspEI BsrDI BsrGI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DraI DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I FbaI FspI HgaI HpaI KspI MamI MfeI MluI MroI MunI NcoI NdeI NheI NotI NruI NspV PacI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI SwaI XbaI XcmI XmnI ################################### # ONE CUT ENZYMES ( 50 enzymes) # ################################### AatII 1818 GACGTC GACGT^C Acc65I 1711 GGTACC G^GTACC AccI 1638 GTAGAC GT^[AC][GT]AC AflIII 2508 ACATGT A^C[GA][CT]GT AgeI 251 ACCGGT A^CCGGT AhdI 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 1786 GGGCCC GGGCC^C Asp718I 1711 GGTACC G^GTACC AspEI 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvrII 1804 CCTAGG C^CTAGG BamHI 2173 GGATCC G^GATCC BbeI 954 GGCGCC GGCGC^C BbsI 2057 GAAGAC GAAGAC(2/6) BlnI 1804 CCTAGG C^CTAGG BpuAI 2057 GAAGAC GAAGAC(2/6) BsaAI 959 CACGTA [CT]AC^GT[GA] BsgI 2212 CTGCAC GTGCAG(16/14) BsmBI 2457 GAGACG CGTCTC(1/5) BspHI 210 TCATGA T^CATGA BspLU11I 2508 ACATGT A^CATGT BspMI 804 ACCTGC ACCTGC(4/8) BsrBI 1082 GAGCGG CCGCTC(-3/-3) BssHII 1427 GCGCGC G^CGCGC BssSI 308 CACGAG CACGAG(-5/-1) BstAPI 2286 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraIII 647 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoRI 1856 GAATTC G^AATTC EcoRV 1283 GATATC GAT^ATC EcoT22I 242 ATGCAT ATGCA^T FseI 1937 GGCCGGCC GGCCGG^CC HindIII 996 AAGCTT A^AGCTT KasI 954 GGCGCC G^GCGCC KpnI 1711 GGTACC GGTAC^C NaeI 1938 GCCGGC GCC^GGC NarI 954 GGCGCC GG^CGCC NgoMIV 1938 GCCGGC G^CCGGC NsiI 242 ATGCAT ATGCA^T PflFI 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC PinAI 251 ACCGGT A^CCGGT PstI 68 CTGCAG CTGCA^G RcaI 210 TCATGA T^CATGA ScaI 509 AGTACT AGT^ACT SfoI 954 GGCGCC GGC^GCC SmaI 618 CCCGGG CCC^GGG StuI 1775 AGGCCT AGG^CCT Tth111I 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 618 CCCGGG C^CCGGG XmaI 618 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### AcsI 725 AAATTC [GA]^AATT[CT] 1856 GAATTC AcyI 954 GGCGCC G[GA]^CG[CT]C 1818 GACGTC Alw44I 568 GTGCAC G^TGCAC 2438 GTGCAC ApaLI 568 GTGCAC G^TGCAC 2438 GTGCAC ApoI 725 AAATTC [GA]^AATT[CT] 1856 GAATTC BcgI 45 GCATCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 802 GCACCTGCTTCG BglI 198 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 766 GCCACGGTGGC BglII 12 AGATCT A^GATCT 2130 AGATCT BlpI 193 GCTGAGC GC^T[ACGT]AGC 2017 GCTGAGC Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC 2017 GCTGAGC BsaHI 954 GGCGCC G[GA]^CG[CT]C 1818 GACGTC BsaWI 251 ACCGGT [AT]^CCGG[AT] 1047 ACCGGA BseRI 110 GAGGAG GAGGAG(10/8) 1079 GAGGAG BsrFI 251 ACCGGT [GA]^CCGG[CT] 1938 GCCGGC BstXI 19 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 540 CCAACGACCTGG CelII 193 GCTGAGC GC^T[ACGT]AGC 2017 GCTGAGC Cfr10I 251 ACCGGT [GA]^CCGG[CT] 1938 GCCGGC EcoNI 1685 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1922 CCTGTGCCAGG HaeII 612 AGCGCC [GA]GCGC^[CT] 954 GGCGCC Hin1I 954 GGCGCC G[GA]^CG[CT]C 1818 GACGTC HincII 818 GTCAAC GT[CT]^[GA]AC 2317 GTTGAC HindII 818 GTCAAC GT[CT]^[GA]AC 2317 GTTGAC PaeR7I 1668 CTCGAG C^TCGAG 1824 CTCGAG PpuMI 1733 AGGTCCC [GA]G^G[AT]CC[CT] 2254 GGGACCC PvuII 322 CAGCTG CAG^CTG 2384 CAGCTG SacI 86 GAGCTC GAGCT^C 1566 GAGCTC SexAI 546 ACCTGGT A^CC[AT]GGT 648 ACCAGGT SphI 244 GCATGC GCATG^C 1890 GCATGC XhoI 1668 CTCGAG C^TCGAG 1824 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BmyI BpmI BsaI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII