##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
AccIII AclI AflII AocI Aor51HI
AscI AseI AsnI Asp700I AviII
BbrPI BbvCI BciVI BclI BfrI
BsaBI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BsrDI BsrGI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DraI DrdI
EagI EclXI Eco47III Eco52I Eco81I
EcoO65I FbaI FspI HgaI HpaI
KspI MamI MfeI MluI MroI
MunI NcoI NdeI NheI NotI
NruI NspV PacI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacII SalI SapI SbfI
SfiI SfuI SgrAI SnaBI SpeI
Sse8387I SspBI SspI SwaI XbaI
XcmI XmnI
###################################
# ONE CUT ENZYMES ( 50 enzymes) #
###################################
AatII 1818 GACGTC GACGT^C
Acc65I 1711 GGTACC G^GTACC
AccI 1638 GTAGAC GT^[AC][GT]AC
AflIII 2508 ACATGT A^C[GA][CT]GT
AgeI 251 ACCGGT A^CCGGT
AhdI 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 1786 GGGCCC GGGCC^C
Asp718I 1711 GGTACC G^GTACC
AspEI 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 1804 CCTAGG C^CTAGG
BamHI 2173 GGATCC G^GATCC
BbeI 954 GGCGCC GGCGC^C
BbsI 2057 GAAGAC GAAGAC(2/6)
BlnI 1804 CCTAGG C^CTAGG
BpuAI 2057 GAAGAC GAAGAC(2/6)
BsaAI 959 CACGTA [CT]AC^GT[GA]
BsgI 2212 CTGCAC GTGCAG(16/14)
BsmBI 2457 GAGACG CGTCTC(1/5)
BspHI 210 TCATGA T^CATGA
BspLU11I 2508 ACATGT A^CATGT
BspMI 804 ACCTGC ACCTGC(4/8)
BsrBI 1082 GAGCGG CCGCTC(-3/-3)
BssHII 1427 GCGCGC G^CGCGC
BssSI 308 CACGAG CACGAG(-5/-1)
BstAPI 2286 GCACCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraIII 647 CACCAGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 545 GACCTGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 1856 GAATTC G^AATTC
EcoRV 1283 GATATC GAT^ATC
EcoT22I 242 ATGCAT ATGCA^T
FseI 1937 GGCCGGCC GGCCGG^CC
HindIII 996 AAGCTT A^AGCTT
KasI 954 GGCGCC G^GCGCC
KpnI 1711 GGTACC GGTAC^C
NaeI 1938 GCCGGC GCC^GGC
NarI 954 GGCGCC GG^CGCC
NgoMIV 1938 GCCGGC G^CCGGC
NsiI 242 ATGCAT ATGCA^T
PflFI 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 251 ACCGGT A^CCGGT
PstI 68 CTGCAG CTGCA^G
RcaI 210 TCATGA T^CATGA
ScaI 509 AGTACT AGT^ACT
SfoI 954 GGCGCC GGC^GCC
SmaI 618 CCCGGG CCC^GGG
StuI 1775 AGGCCT AGG^CCT
Tth111I 1136 GACTCAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 618 CCCGGG C^CCGGG
XmaI 618 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AcsI 725 AAATTC [GA]^AATT[CT]
1856 GAATTC
AcyI 954 GGCGCC G[GA]^CG[CT]C
1818 GACGTC
Alw44I 568 GTGCAC G^TGCAC
2438 GTGCAC
ApaLI 568 GTGCAC G^TGCAC
2438 GTGCAC
ApoI 725 AAATTC [GA]^AATT[CT]
1856 GAATTC
BcgI 45 GCATCAACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
802 GCACCTGCTTCG
BglI 198 GCCCCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
766 GCCACGGTGGC
BglII 12 AGATCT A^GATCT
2130 AGATCT
BlpI 193 GCTGAGC GC^T[ACGT]AGC
2017 GCTGAGC
Bpu1102I 193 GCTGAGC GC^T[ACGT]AGC
2017 GCTGAGC
BsaHI 954 GGCGCC G[GA]^CG[CT]C
1818 GACGTC
BsaWI 251 ACCGGT [AT]^CCGG[AT]
1047 ACCGGA
BseRI 110 GAGGAG GAGGAG(10/8)
1079 GAGGAG
BsrFI 251 ACCGGT [GA]^CCGG[CT]
1938 GCCGGC
BstXI 19 CCAGAAAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
540 CCAACGACCTGG
CelII 193 GCTGAGC GC^T[ACGT]AGC
2017 GCTGAGC
Cfr10I 251 ACCGGT [GA]^CCGG[CT]
1938 GCCGGC
EcoNI 1685 CCTCCCAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1922 CCTGTGCCAGG
HaeII 612 AGCGCC [GA]GCGC^[CT]
954 GGCGCC
Hin1I 954 GGCGCC G[GA]^CG[CT]C
1818 GACGTC
HincII 818 GTCAAC GT[CT]^[GA]AC
2317 GTTGAC
HindII 818 GTCAAC GT[CT]^[GA]AC
2317 GTTGAC
PaeR7I 1668 CTCGAG C^TCGAG
1824 CTCGAG
PpuMI 1733 AGGTCCC [GA]G^G[AT]CC[CT]
2254 GGGACCC
PvuII 322 CAGCTG CAG^CTG
2384 CAGCTG
SacI 86 GAGCTC GAGCT^C
1566 GAGCTC
SexAI 546 ACCTGGT A^CC[AT]GGT
648 ACCAGGT
SphI 244 GCATGC GCATG^C
1890 GCATGC
XhoI 1668 CTCGAG C^TCGAG
1824 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaI BsaJI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CfoI Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PflMI PleI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII