################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII Acc65I AccI AccII AcyI AflII AflIII AgeI AhdI Alw44I Aor51HI ApaI ApaLI AscI AseI AsnI Asp718I AspEI AspI AviII AvrII BbeI BbrPI BfrI BlnI BsaAI BsaBI BsaHI BsiWI BsmBI BsmI BspDI BspLU11I BssHII BssSI Bst1107I BstBI BstEII BstPI BstUI BstZ17I ClaI CpoI DraI DraIII EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI EcoRV FseI FspI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MunI MvnI NarI NdeI NotI NruI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshBI RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I ################################### # ONE CUT ENZYMES ( 52 enzymes) # ################################### AccIII 1687 TCCGGA T^CCGGA AclI 2592 AACGTT AA^CGTT AocI 2654 CCTAAGG CC^T[ACGT]AGG Asp700I 1585 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC BanI 1303 GGTGCC G^G[CT][GA]CC BbvCI 606 CCTCAGC CCTCAGC(-5/-2) BciVI 2299 GGATAC GTATCC(6/5) BclI 1641 TGATCA T^GATCA BlpI 2506 GCTCAGC GC^T[ACGT]AGC Bpu1102I 2506 GCTCAGC GC^T[ACGT]AGC BsaWI 1687 TCCGGA [AT]^CCGG[AT] BseAI 1687 TCCGGA T^CCGGA BsiEI 799 CGATCG CG[GA][CT]^CG Bsp1407I 2169 TGTACA T^GTACA BspEI 1687 TCCGGA T^CCGGA BspHI 480 TCATGA T^CATGA BsrBI 2504 CCGCTC CCGCTC(-3/-3) BsrFI 2152 GCCGGC [GA]^CCGG[CT] BsrGI 2169 TGTACA T^GTACA BstXI 675 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 2654 CCTAAGG CC^T[ACGT]AGG CelII 2506 GCTCAGC GC^T[ACGT]AGC Cfr10I 2152 GCCGGC [GA]^CCGG[CT] DrdI 163 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 312 CCATGG C^C[GA][CT]GG Eco81I 2654 CCTAAGG CC^T[ACGT]AGG EcoT22I 1634 ATGCAT ATGCA^T FbaI 1641 TGATCA T^GATCA HaeII 141 GGCGCT [GA]GCGC^[CT] MroI 1687 TCCGGA T^CCGGA NaeI 2152 GCCGGC GCC^GGC NcoI 312 CCATGG C^CATGG NgoMIV 2152 GCCGGC G^CCGGC NheI 1537 GCTAGC G^CTAGC NsiI 1634 ATGCAT ATGCA^T PaeR7I 2429 CTCGAG C^TCGAG PflMI 964 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PpuMI 301 AGGTCCC [GA]G^G[AT]CC[CT] PshAI 1057 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC Psp1406I 2592 AACGTT AA^CGTT PvuI 799 CGATCG CGAT^CG RcaI 480 TCATGA T^CATGA SfiI 1030 GGCCCCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 762 CCCGGG CCC^GGG SphI 552 GCATGC GCATG^C SspBI 2169 TGTACA T^GTACA Van91I 964 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 460 TCTAGA T^CTAGA XhoI 2429 CTCGAG C^TCGAG XmaCI 762 CCCGGG C^CCGGG XmaI 762 CCCGGG C^CCGGG XmnI 1585 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcsI 1790 GAATTT [GA]^AATT[CT] 1868 AAATTT ApoI 1790 GAATTT [GA]^AATT[CT] 1868 AAATTT BalI 1038 TGGCCA TGG^CCA 2485 TGGCCA BamHI 78 GGATCC G^GATCC 1889 GGATCC BbsI 1585 GAAGAC GAAGAC(2/6) 2291 GAAGAC BcgI 799 CGATCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 1428 CGAAGTGCCTGC BpuAI 1585 GAAGAC GAAGAC(2/6) 2291 GAAGAC BsaI 1478 GAGACC GGTCTC(1/5) 1063 GGTCTC BsgI 2551 CTGCAC GTGCAG(16/14) 2060 GTGCAG BsmAI 1478 GAGAC GTCTC(1/5) 1064 GTCTC BsrDI 358 CATTGC GCAATG(2/0) 805 CATTGC EaeI 1038 TGGCCA [CT]^GGCC[GA] 2485 TGGCCA EcoNI 520 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 583 CCTCCCCCAGG MaeII 2058 ACGT A^CGT 2593 ACGT MluNI 1038 TGGCCA TGG^CCA 2485 TGGCCA MscI 1038 TGGCCA TGG^CCA 2485 TGGCCA MseI 2360 TTAA T^TAA 2671 TTAA MslI 676 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1627 CACAGAAATG NspI 552 GCATGC [GA]CATG^[CT] 1455 ACATGC SacI 476 GAGCTC GAGCT^C 834 GAGCTC Tru9I 2360 TTAA T^TAA 2671 TTAA ######################################### # 3 OR MORE CUT ENZYMES ( 90 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII BanII BbvI BcnI BfaI BglI BglII BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I XcmI XhoII