##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII Acc65I AccI AccII AcyI
AflII AflIII AgeI AhdI Alw44I
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbeI BbrPI BfrI BlnI
BsaAI BsaBI BsaHI BsiWI BsmBI
BsmI BspDI BspLU11I BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstZ17I ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FseI FspI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MunI MvnI NarI NdeI NotI
NruI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshBI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AccIII 1687 TCCGGA T^CCGGA
AclI 2592 AACGTT AA^CGTT
AocI 2654 CCTAAGG CC^T[ACGT]AGG
Asp700I 1585 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanI 1303 GGTGCC G^G[CT][GA]CC
BbvCI 606 CCTCAGC CCTCAGC(-5/-2)
BciVI 2299 GGATAC GTATCC(6/5)
BclI 1641 TGATCA T^GATCA
BlpI 2506 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2506 GCTCAGC GC^T[ACGT]AGC
BsaWI 1687 TCCGGA [AT]^CCGG[AT]
BseAI 1687 TCCGGA T^CCGGA
BsiEI 799 CGATCG CG[GA][CT]^CG
Bsp1407I 2169 TGTACA T^GTACA
BspEI 1687 TCCGGA T^CCGGA
BspHI 480 TCATGA T^CATGA
BsrBI 2504 CCGCTC CCGCTC(-3/-3)
BsrFI 2152 GCCGGC [GA]^CCGG[CT]
BsrGI 2169 TGTACA T^GTACA
BstXI 675 CCACCCCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 2654 CCTAAGG CC^T[ACGT]AGG
CelII 2506 GCTCAGC GC^T[ACGT]AGC
Cfr10I 2152 GCCGGC [GA]^CCGG[CT]
DrdI 163 GACTCTGCAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 312 CCATGG C^C[GA][CT]GG
Eco81I 2654 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1634 ATGCAT ATGCA^T
FbaI 1641 TGATCA T^GATCA
HaeII 141 GGCGCT [GA]GCGC^[CT]
MroI 1687 TCCGGA T^CCGGA
NaeI 2152 GCCGGC GCC^GGC
NcoI 312 CCATGG C^CATGG
NgoMIV 2152 GCCGGC G^CCGGC
NheI 1537 GCTAGC G^CTAGC
NsiI 1634 ATGCAT ATGCA^T
PaeR7I 2429 CTCGAG C^TCGAG
PflMI 964 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 301 AGGTCCC [GA]G^G[AT]CC[CT]
PshAI 1057 GACTCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 2592 AACGTT AA^CGTT
PvuI 799 CGATCG CGAT^CG
RcaI 480 TCATGA T^CATGA
SfiI 1030 GGCCCCATTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 762 CCCGGG CCC^GGG
SphI 552 GCATGC GCATG^C
SspBI 2169 TGTACA T^GTACA
Van91I 964 CCAAAAACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 460 TCTAGA T^CTAGA
XhoI 2429 CTCGAG C^TCGAG
XmaCI 762 CCCGGG C^CCGGG
XmaI 762 CCCGGG C^CCGGG
XmnI 1585 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1790 GAATTT [GA]^AATT[CT]
1868 AAATTT
ApoI 1790 GAATTT [GA]^AATT[CT]
1868 AAATTT
BalI 1038 TGGCCA TGG^CCA
2485 TGGCCA
BamHI 78 GGATCC G^GATCC
1889 GGATCC
BbsI 1585 GAAGAC GAAGAC(2/6)
2291 GAAGAC
BcgI 799 CGATCGCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
1428 CGAAGTGCCTGC
BpuAI 1585 GAAGAC GAAGAC(2/6)
2291 GAAGAC
BsaI 1478 GAGACC GGTCTC(1/5)
1063 GGTCTC
BsgI 2551 CTGCAC GTGCAG(16/14)
2060 GTGCAG
BsmAI 1478 GAGAC GTCTC(1/5)
1064 GTCTC
BsrDI 358 CATTGC GCAATG(2/0)
805 CATTGC
EaeI 1038 TGGCCA [CT]^GGCC[GA]
2485 TGGCCA
EcoNI 520 CCTGGAAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
583 CCTCCCCCAGG
MaeII 2058 ACGT A^CGT
2593 ACGT
MluNI 1038 TGGCCA TGG^CCA
2485 TGGCCA
MscI 1038 TGGCCA TGG^CCA
2485 TGGCCA
MseI 2360 TTAA T^TAA
2671 TTAA
MslI 676 CACCCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1627 CACAGAAATG
NspI 552 GCATGC [GA]CATG^[CT]
1455 ACATGC
SacI 476 GAGCTC GAGCT^C
834 GAGCTC
Tru9I 2360 TTAA T^TAA
2671 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanII BbvI
BcnI BfaI BglI BglII BmyI
BpmI BsaJI BseRI BsiHKAI BsiYI
BslI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstAPI BstF5I BstNI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StuI
StyI TaqI TfiI TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII