##################################
# NO CUT ENZYMES ( 107 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AvrII BamHI BbeI BbrPI
BcgI BclI BfrI BglII BlnI
BsaWI BseAI BsiEI BsiWI Bsp1407I
BspDI BspEI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
ClaI CpoI DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FbaI FseI
HindIII HpaI KasI KpnI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 38 enzymes) #
###################################
AccI 1445 GTAGAC GT^[AC][GT]AC
AcsI 163 GAATTC [GA]^AATT[CT]
AflIII 346 ACATGT A^C[GA][CT]GT
AocI 2283 CCTGAGG CC^T[ACGT]AGG
ApoI 163 GAATTC [GA]^AATT[CT]
AspI 2165 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1047 TGCGCA TGC^GCA
BbsI 1466 GTCTTC GAAGAC(2/6)
BbvCI 6 GCTGAGG CCTCAGC(-5/-2)
BciVI 1781 GGATAC GTATCC(6/5)
BpuAI 1466 GTCTTC GAAGAC(2/6)
BsaAI 2004 TACGTG [CT]AC^GT[GA]
BsaBI 2009 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 1151 CTCCTC GAGGAG(10/8)
BsgI 1133 CTGCAC GTGCAG(16/14)
BsmBI 1795 GAGACG CGTCTC(1/5)
BsmI 1173 GCATTC GAATGC(1/-1)
BspLU11I 346 ACATGT A^CATGT
BsrDI 645 GCAATG GCAATG(2/0)
BssHII 730 GCGCGC G^CGCGC
BstYI 1689 AGATCC [GA]^GATC[CT]
Bsu36I 2283 CCTGAGG CC^T[ACGT]AGG
DraI 40 TTTAAA TTT^AAA
Eco81I 2283 CCTGAGG CC^T[ACGT]AGG
EcoRI 163 GAATTC G^AATTC
FspI 1047 TGCGCA TGC^GCA
HaeII 1281 AGCGCC [GA]GCGC^[CT]
HincII 418 GTTGAC GT[CT]^[GA]AC
HindII 418 GTTGAC GT[CT]^[GA]AC
MamI 2009 GATGGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 1689 AGATCC [GA]^GATC[CT]
NcoI 1344 CCATGG C^CATGG
PflFI 2165 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 1026 AGGACCC [GA]G^G[AT]CC[CT]
SphI 941 GCATGC GCATG^C
Tth111I 2165 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 195 CCAAGCAGCTAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 1689 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AccII 731 CGCG CG^CG
982 CGCG
AcyI 628 GACGCC G[GA]^CG[CT]C
1001 GACGCC
AlwNI 380 CAGCCCCTG CAG[ACGT][ACGT][ACGT]^CTG
758 CAGTTTCTG
ApaI 1110 GGGCCC GGGCC^C
1547 GGGCCC
AvaI 1557 CCCGAG C^[CT]CG[GA]G
2160 CCCGAG
BlpI 175 GCTGAGC GC^T[ACGT]AGC
428 GCTCAGC
Bpu1102I 175 GCTGAGC GC^T[ACGT]AGC
428 GCTCAGC
BsaHI 628 GACGCC G[GA]^CG[CT]C
1001 GACGCC
BsoBI 1557 CCCGAG C^[CT]CG[GA]G
2160 CCCGAG
BspHI 769 TCATGA T^CATGA
1716 TCATGA
BstUI 731 CGCG CG^CG
982 CGCG
BstXI 1059 CCAACCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1096 CCAAGACAATGG
CelII 175 GCTGAGC GC^T[ACGT]AGC
428 GCTCAGC
DsaI 273 CCACGG C^C[GA][CT]GG
1344 CCATGG
EarI 188 GAAGAG CTCTTC(1/4)
367 GAAGAG
Hin1I 628 GACGCC G[GA]^CG[CT]C
1001 GACGCC
Ksp632I 188 GAAGAG CTCTTC(1/4)
367 GAAGAG
MaeII 474 ACGT A^CGT
2005 ACGT
MseI 41 TTAA T^TAA
1746 TTAA
MvnI 731 CGCG CG^CG
982 CGCG
PstI 377 CTGCAG CTGCA^G
845 CTGCAG
RcaI 769 TCATGA T^CATGA
1716 TCATGA
SfcI 377 CTGCAG C^T[GA][CT]AG
845 CTGCAG
SmlI 869 CTCAAG C^T[CT][GA]AG
1277 CTCAAG
Tru9I 41 TTAA T^TAA
1746 TTAA
Tsp45I 482 GTGAC ^GT[GC]AC
2100 GTGAC
Tsp509I 164 AATT ^AATT
1675 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AciI AfaI AluI AlwI AspHI
AvaII BalI BanI BanII BbvI
BcnI BfaI BglI BmyI BpmI
BsaI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfiI StuI
StyI TaqI TfiI TseI TspRI
TthHB8I