##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII AccIII AclI AcyI AflII
AflIII AgeI Alw44I ApaLI AscI
AseI AsnI AspI AviII BalI
BbeI BbrPI BcgI BciVI BfrI
BglI BglII BsaBI BsaHI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrGI BssHII Bst1107I BstBI BstXI
BstZ17I ClaI CpoI DrdI EaeI
EagI EclXI Eco52I EcoRI EcoRV
EcoT22I FseI FspI Hin1I KasI
KspI MamI MfeI MluI MluNI
MroI MscI MunI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SphI Sse8387I SspBI SspI StuI
SwaI Tth111I XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
Acc65I 1565 GGTACC G^GTACC
AccI 1001 GTAGAC GT^[AC][GT]AC
AhdI 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1261 CCTGAGG CC^T[ACGT]AGG
Aor51HI 487 AGCGCT AGC^GCT
ApaI 703 GGGCCC GGGCC^C
Asp718I 1565 GGTACC G^GTACC
AspEI 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 2038 GGATCC G^GATCC
BcnI 886 CCGGG CC^[GC]GG
BsaAI 2310 TACGTA [CT]AC^GT[GA]
BsaWI 1187 TCCGGT [AT]^CCGG[AT]
BsrDI 474 CATTGC GCAATG(2/0)
BsrFI 429 GCCGGT [GA]^CCGG[CT]
BssSI 1793 CTCGTG CACGAG(-5/-1)
BstEII 522 GGTGACC G^GT[ACGT]ACC
BstPI 522 GGTGACC G^GT[ACGT]ACC
BstYI 2038 GGATCC [GA]^GATC[CT]
Bsu36I 1261 CCTGAGG CC^T[ACGT]AGG
Cfr10I 429 GCCGGT [GA]^CCGG[CT]
DraIII 1618 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG
Eam1105I 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 1246 CTCTTC CTCTTC(1/4)
Eco47III 487 AGCGCT AGC^GCT
Eco81I 1261 CCTGAGG CC^T[ACGT]AGG
EcoNI 1569 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 522 GGTGACC G^GT[ACGT]ACC
FauI 231 CCCGC CCCGC(4/6)
HpaI 1217 GTTAAC GTT^AAC
KpnI 1565 GGTACC GGTAC^C
Ksp632I 1246 CTCTTC CTCTTC(1/4)
MflI 2038 GGATCC [GA]^GATC[CT]
NciI 886 CCGGG CC^[GC]GG
NspI 168 GCATGT [GA]CATG^[CT]
PaeR7I 283 CTCGAG C^TCGAG
SacI 688 GAGCTC GAGCT^C
SfaNI 216 GATGC GCATC(5/9)
SnaBI 2310 TACGTA TAC^GTA
SpeI 1221 ACTAGT A^CTAGT
XhoI 283 CTCGAG C^TCGAG
XhoII 2038 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AcsI 298 GAATTT [GA]^AATT[CT]
986 AAATTC
ApoI 298 GAATTT [GA]^AATT[CT]
986 AAATTC
Asp700I 783 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1687 GAAGGACTTC
BanI 1565 GGTACC G^G[CT][GA]CC
2281 GGCACC
BbsI 1296 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BbvCI 96 CCTCAGC CCTCAGC(-5/-2)
1569 CCTCAGC
BlpI 1347 GCTCAGC GC^T[ACGT]AGC
1771 GCTCAGC
Bpu1102I 1347 GCTCAGC GC^T[ACGT]AGC
1771 GCTCAGC
BpuAI 1296 GAAGAC GAAGAC(2/6)
1267 GTCTTC
BsaI 849 GAGACC GGTCTC(1/5)
1283 GGTCTC
CelII 1347 GCTCAGC GC^T[ACGT]AGC
1771 GCTCAGC
DraI 2196 TTTAAA TTT^AAA
2234 TTTAAA
DsaI 293 CCATGG C^C[GA][CT]GG
2336 CCATGG
HaeII 487 AGCGCT [GA]GCGC^[CT]
1659 GGCGCT
HgaI 331 GACGC GACGC(5/10)
946 GCGTC
HindIII 539 AAGCTT A^AGCTT
922 AAGCTT
HphI 522 GGTGA GGTGA(8/7)
1533 TCACC
MaeII 2311 ACGT A^CGT
2392 ACGT
NcoI 293 CCATGG C^CATGG
2336 CCATGG
PstI 1493 CTGCAG CTGCA^G
1640 CTGCAG
PvuII 1330 CAGCTG CAG^CTG
1737 CAGCTG
SmlI 283 CTCGAG C^T[CT][GA]AG
844 CTTGAG
TaqI 273 TCGA T^CGA
284 TCGA
TfiI 325 GAATC G^A[AT]TC
407 GATTC
TthHB8I 273 TCGA T^CGA
284 TCGA
XbaI 1727 TCTAGA T^CTAGA
2155 TCTAGA
XcmI 293 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
865 CCAGCAGCAGTTTGG
XmnI 783 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1687 GAAGGACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 85 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII AvrII
BanII BbvI BclI BfaI BlnI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EcoO109I EcoRII EcoT14I FbaI Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HincII HindII HinfI HpaII ItaI
MaeI MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NdeII NlaIII NlaIV
PflMI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfcI StyI Tru9I
TseI Tsp45I Tsp509I TspRI Van91I