################################## # NO CUT ENZYMES ( 104 enzymes) # ################################## AatII AccIII AclI AcyI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI AspI AviII BalI BbeI BbrPI BcgI BciVI BfrI BglI BglII BsaBI BsaHI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrBI BsrGI BssHII Bst1107I BstBI BstXI BstZ17I ClaI CpoI DrdI EaeI EagI EclXI Eco52I EcoRI EcoRV EcoT22I FseI FspI Hin1I KasI KspI MamI MfeI MluI MluNI MroI MscI MunI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI NspV PacI PflFI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SapI SbfI ScaI SexAI SfiI SfoI SfuI SgrAI SmaI SphI Sse8387I SspBI SspI StuI SwaI Tth111I XmaCI XmaI ################################### # ONE CUT ENZYMES ( 41 enzymes) # ################################### Acc65I 1565 GGTACC G^GTACC AccI 1001 GTAGAC GT^[AC][GT]AC AhdI 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 1261 CCTGAGG CC^T[ACGT]AGG Aor51HI 487 AGCGCT AGC^GCT ApaI 703 GGGCCC GGGCC^C Asp718I 1565 GGTACC G^GTACC AspEI 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BamHI 2038 GGATCC G^GATCC BcnI 886 CCGGG CC^[GC]GG BsaAI 2310 TACGTA [CT]AC^GT[GA] BsaWI 1187 TCCGGT [AT]^CCGG[AT] BsrDI 474 CATTGC GCAATG(2/0) BsrFI 429 GCCGGT [GA]^CCGG[CT] BssSI 1793 CTCGTG CACGAG(-5/-1) BstEII 522 GGTGACC G^GT[ACGT]ACC BstPI 522 GGTGACC G^GT[ACGT]ACC BstYI 2038 GGATCC [GA]^GATC[CT] Bsu36I 1261 CCTGAGG CC^T[ACGT]AGG Cfr10I 429 GCCGGT [GA]^CCGG[CT] DraIII 1618 CACACTGTG CAC[ACGT][ACGT][ACGT]^GTG Eam1105I 749 GACACTAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EarI 1246 CTCTTC CTCTTC(1/4) Eco47III 487 AGCGCT AGC^GCT Eco81I 1261 CCTGAGG CC^T[ACGT]AGG EcoNI 1569 CCTCAGCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 522 GGTGACC G^GT[ACGT]ACC FauI 231 CCCGC CCCGC(4/6) HpaI 1217 GTTAAC GTT^AAC KpnI 1565 GGTACC GGTAC^C Ksp632I 1246 CTCTTC CTCTTC(1/4) MflI 2038 GGATCC [GA]^GATC[CT] NciI 886 CCGGG CC^[GC]GG NspI 168 GCATGT [GA]CATG^[CT] PaeR7I 283 CTCGAG C^TCGAG SacI 688 GAGCTC GAGCT^C SfaNI 216 GATGC GCATC(5/9) SnaBI 2310 TACGTA TAC^GTA SpeI 1221 ACTAGT A^CTAGT XhoI 283 CTCGAG C^TCGAG XhoII 2038 GGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AcsI 298 GAATTT [GA]^AATT[CT] 986 AAATTC ApoI 298 GAATTT [GA]^AATT[CT] 986 AAATTC Asp700I 783 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1687 GAAGGACTTC BanI 1565 GGTACC G^G[CT][GA]CC 2281 GGCACC BbsI 1296 GAAGAC GAAGAC(2/6) 1267 GTCTTC BbvCI 96 CCTCAGC CCTCAGC(-5/-2) 1569 CCTCAGC BlpI 1347 GCTCAGC GC^T[ACGT]AGC 1771 GCTCAGC Bpu1102I 1347 GCTCAGC GC^T[ACGT]AGC 1771 GCTCAGC BpuAI 1296 GAAGAC GAAGAC(2/6) 1267 GTCTTC BsaI 849 GAGACC GGTCTC(1/5) 1283 GGTCTC CelII 1347 GCTCAGC GC^T[ACGT]AGC 1771 GCTCAGC DraI 2196 TTTAAA TTT^AAA 2234 TTTAAA DsaI 293 CCATGG C^C[GA][CT]GG 2336 CCATGG HaeII 487 AGCGCT [GA]GCGC^[CT] 1659 GGCGCT HgaI 331 GACGC GACGC(5/10) 946 GCGTC HindIII 539 AAGCTT A^AGCTT 922 AAGCTT HphI 522 GGTGA GGTGA(8/7) 1533 TCACC MaeII 2311 ACGT A^CGT 2392 ACGT NcoI 293 CCATGG C^CATGG 2336 CCATGG PstI 1493 CTGCAG CTGCA^G 1640 CTGCAG PvuII 1330 CAGCTG CAG^CTG 1737 CAGCTG SmlI 283 CTCGAG C^T[CT][GA]AG 844 CTTGAG TaqI 273 TCGA T^CGA 284 TCGA TfiI 325 GAATC G^A[AT]TC 407 GATTC TthHB8I 273 TCGA T^CGA 284 TCGA XbaI 1727 TCTAGA T^CTAGA 2155 TCTAGA XcmI 293 CCATGGAATTTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 865 CCAGCAGCAGTTTGG XmnI 783 GAAGTGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1687 GAAGGACTTC ######################################### # 3 OR MORE CUT ENZYMES ( 85 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI AspHI AvaI AvaII AvrII BanII BbvI BclI BfaI BlnI BmyI BpmI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FbaI Fnu4HI FokI HaeIII HapII HhaI HinP1I HincII HindII HinfI HpaII ItaI MaeI MaeIII MboI MboII MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NdeII NlaIII NlaIV PflMI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfcI StyI Tru9I TseI Tsp45I Tsp509I TspRI Van91I