################################## # NO CUT ENZYMES ( 82 enzymes) # ################################## Acc65I AccI AccIII AclI AflII AgeI AhdI Aor51HI AseI AsnI Asp718I AspEI AvrII BamHI BbeI BbsI BfrI BlnI BpuAI BsaBI BseAI BsiWI Bsp1407I BspDI BspEI BsrFI BsrGI BssSI Bst1107I BstZ17I Cfr10I ClaI CpoI DraI DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoRV FseI HincII HindII HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NaeI NarI NgoMIV NotI NruI PacI PinAI PmeI PshAI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SexAI SfoI SgrAI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI XcmI ################################### # ONE CUT ENZYMES ( 45 enzymes) # ################################### AatII 349 GACGTC GACGT^C AflIII 1612 ACATGT A^C[GA][CT]GT ApaI 127 GGGCCC GGGCC^C AscI 30 GGCGCGCC GG^CGCGCC AspI 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1200 TGCGCA TGC^GCA BanI 851 GGTGCC G^G[CT][GA]CC BbrPI 2231 CACGTG CAC^GTG BcgI 1444 GCACTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 873 TGATCA T^GATCA BglII 2254 AGATCT A^GATCT BsaWI 150 TCCGGT [AT]^CCGG[AT] BsgI 1724 GTGCAG GTGCAG(16/14) BsiEI 94 CGGTCG CG[GA][CT]^CG BsmBI 628 CGTCTC CGTCTC(1/5) BspHI 223 TCATGA T^CATGA BspLU11I 1612 ACATGT A^CATGT BsrBI 22 GAGCGG CCGCTC(-3/-3) BsrDI 963 GCAATG GCAATG(2/0) BssHII 31 GCGCGC G^CGCGC BstAPI 1867 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1548 TTCGAA TT^CGAA DraIII 659 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 437 CTCTTC CTCTTC(1/4) FbaI 873 TGATCA T^GATCA FspI 1200 TGCGCA TGC^GCA Ksp632I 437 CTCTTC CTCTTC(1/4) NdeI 2186 CATATG CA^TATG NheI 2061 GCTAGC G^CTAGC NspV 1548 TTCGAA TT^CGAA PaeR7I 2088 CTCGAG C^TCGAG PflFI 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 1396 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 2231 CACGTG CAC^GTG PmlI 2231 CACGTG CAC^GTG PpuMI 2114 AGGTCCC [GA]G^G[AT]CC[CT] RcaI 223 TCATGA T^CATGA SacI 434 GAGCTC GAGCT^C SapI 436 GCTCTTC GCTCTTC(1/4) SfiI 1135 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SfuI 1548 TTCGAA TT^CGAA Tth111I 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 1396 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XbaI 1834 TCTAGA T^CTAGA XhoI 2088 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AfaI 154 GTAC GT^AC 1316 GTAC AlwNI 920 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG 1868 CAGTCCCTG AocI 738 CCTTAGG CC^T[ACGT]AGG 1046 CCTAAGG BbvCI 1210 CCTCAGC CCTCAGC(-5/-2) 1799 GCTGAGG BciVI 2046 GGATAC GTATCC(6/5) 300 GTATCC BlpI 73 GCTGAGC GC^T[ACGT]AGC 1183 GCTGAGC Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC 1183 GCTGAGC BsaAI 752 TACGTG [CT]AC^GT[GA] 2231 CACGTG BsaI 1814 GAGACC GGTCTC(1/5) 2071 GAGACC BseRI 515 GAGGAG GAGGAG(10/8) 1479 GAGGAG BsmI 482 GCATTC GAATGC(1/-1) 1222 GCATTC BstEII 260 GGTGACC G^GT[ACGT]ACC 1105 GGTTACC BstPI 260 GGTGACC G^GT[ACGT]ACC 1105 GGTTACC BstYI 678 AGATCC [GA]^GATC[CT] 2254 AGATCT Bsu36I 738 CCTTAGG CC^T[ACGT]AGG 1046 CCTAAGG CelII 73 GCTGAGC GC^T[ACGT]AGC 1183 GCTGAGC DraII 126 GGGGCCC [GA]G^G[ACGT]CC[CT] 2114 AGGTCCC Eco81I 738 CCTTAGG CC^T[ACGT]AGG 1046 CCTAAGG EcoO109I 126 GGGGCCC [GA]G^G[ACGT]CC[CT] 2114 AGGTCCC EcoO65I 260 GGTGACC G^GT[ACGT]ACC 1105 GGTTACC EcoRI 822 GAATTC G^AATTC 947 GAATTC EcoT22I 1220 ATGCAT ATGCA^T 2183 ATGCAT HaeII 10 AGCGCC [GA]GCGC^[CT] 1187 AGCGCC MflI 678 AGATCC [GA]^GATC[CT] 2254 AGATCT NcoI 837 CCATGG C^CATGG 1569 CCATGG NsiI 1220 ATGCAT ATGCA^T 2183 ATGCAT RsaI 154 GTAC GT^AC 1316 GTAC SmaI 14 CCCGGG CCC^GGG 2099 CCCGGG SphI 42 GCATGC GCATG^C 243 GCATGC Tsp45I 261 GTGAC ^GT[GC]AC 1971 GTGAC XhoII 678 AGATCC [GA]^GATC[CT] 2254 AGATCT XmaCI 14 CCCGGG C^CCGGG 2099 CCCGGG XmaI 14 CCCGGG C^CCGGG 2099 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 98 enzymes) # ######################################### AccII AciI AcsI AcyI AluI Alw44I AlwI ApaLI ApoI Asp700I AspHI AvaI AvaII BalI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaJI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstUI BstXI Cac8I CfoI Cfr13I DdeI DpnII DsaI EaeI EcoNI EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp509I TspRI TthHB8I XmnI