##################################
# NO CUT ENZYMES ( 82 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
AgeI AhdI Aor51HI AseI AsnI
Asp718I AspEI AvrII BamHI BbeI
BbsI BfrI BlnI BpuAI BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BsrFI BsrGI BssSI Bst1107I BstZ17I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoRV FseI HincII HindII HindIII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NgoMIV NotI NruI PacI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SacII SalI SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI XcmI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AatII 349 GACGTC GACGT^C
AflIII 1612 ACATGT A^C[GA][CT]GT
ApaI 127 GGGCCC GGGCC^C
AscI 30 GGCGCGCC GG^CGCGCC
AspI 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1200 TGCGCA TGC^GCA
BanI 851 GGTGCC G^G[CT][GA]CC
BbrPI 2231 CACGTG CAC^GTG
BcgI 1444 GCACTGCGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 873 TGATCA T^GATCA
BglII 2254 AGATCT A^GATCT
BsaWI 150 TCCGGT [AT]^CCGG[AT]
BsgI 1724 GTGCAG GTGCAG(16/14)
BsiEI 94 CGGTCG CG[GA][CT]^CG
BsmBI 628 CGTCTC CGTCTC(1/5)
BspHI 223 TCATGA T^CATGA
BspLU11I 1612 ACATGT A^CATGT
BsrBI 22 GAGCGG CCGCTC(-3/-3)
BsrDI 963 GCAATG GCAATG(2/0)
BssHII 31 GCGCGC G^CGCGC
BstAPI 1867 GCAGTCCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1548 TTCGAA TT^CGAA
DraIII 659 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 437 CTCTTC CTCTTC(1/4)
FbaI 873 TGATCA T^GATCA
FspI 1200 TGCGCA TGC^GCA
Ksp632I 437 CTCTTC CTCTTC(1/4)
NdeI 2186 CATATG CA^TATG
NheI 2061 GCTAGC G^CTAGC
NspV 1548 TTCGAA TT^CGAA
PaeR7I 2088 CTCGAG C^TCGAG
PflFI 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1396 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 2231 CACGTG CAC^GTG
PmlI 2231 CACGTG CAC^GTG
PpuMI 2114 AGGTCCC [GA]G^G[AT]CC[CT]
RcaI 223 TCATGA T^CATGA
SacI 434 GAGCTC GAGCT^C
SapI 436 GCTCTTC GCTCTTC(1/4)
SfiI 1135 GGCCACTCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfuI 1548 TTCGAA TT^CGAA
Tth111I 1816 GACCCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1396 CCAGAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 1834 TCTAGA T^CTAGA
XhoI 2088 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AfaI 154 GTAC GT^AC
1316 GTAC
AlwNI 920 CAGTATCTG CAG[ACGT][ACGT][ACGT]^CTG
1868 CAGTCCCTG
AocI 738 CCTTAGG CC^T[ACGT]AGG
1046 CCTAAGG
BbvCI 1210 CCTCAGC CCTCAGC(-5/-2)
1799 GCTGAGG
BciVI 2046 GGATAC GTATCC(6/5)
300 GTATCC
BlpI 73 GCTGAGC GC^T[ACGT]AGC
1183 GCTGAGC
Bpu1102I 73 GCTGAGC GC^T[ACGT]AGC
1183 GCTGAGC
BsaAI 752 TACGTG [CT]AC^GT[GA]
2231 CACGTG
BsaI 1814 GAGACC GGTCTC(1/5)
2071 GAGACC
BseRI 515 GAGGAG GAGGAG(10/8)
1479 GAGGAG
BsmI 482 GCATTC GAATGC(1/-1)
1222 GCATTC
BstEII 260 GGTGACC G^GT[ACGT]ACC
1105 GGTTACC
BstPI 260 GGTGACC G^GT[ACGT]ACC
1105 GGTTACC
BstYI 678 AGATCC [GA]^GATC[CT]
2254 AGATCT
Bsu36I 738 CCTTAGG CC^T[ACGT]AGG
1046 CCTAAGG
CelII 73 GCTGAGC GC^T[ACGT]AGC
1183 GCTGAGC
DraII 126 GGGGCCC [GA]G^G[ACGT]CC[CT]
2114 AGGTCCC
Eco81I 738 CCTTAGG CC^T[ACGT]AGG
1046 CCTAAGG
EcoO109I 126 GGGGCCC [GA]G^G[ACGT]CC[CT]
2114 AGGTCCC
EcoO65I 260 GGTGACC G^GT[ACGT]ACC
1105 GGTTACC
EcoRI 822 GAATTC G^AATTC
947 GAATTC
EcoT22I 1220 ATGCAT ATGCA^T
2183 ATGCAT
HaeII 10 AGCGCC [GA]GCGC^[CT]
1187 AGCGCC
MflI 678 AGATCC [GA]^GATC[CT]
2254 AGATCT
NcoI 837 CCATGG C^CATGG
1569 CCATGG
NsiI 1220 ATGCAT ATGCA^T
2183 ATGCAT
RsaI 154 GTAC GT^AC
1316 GTAC
SmaI 14 CCCGGG CCC^GGG
2099 CCCGGG
SphI 42 GCATGC GCATG^C
243 GCATGC
Tsp45I 261 GTGAC ^GT[GC]AC
1971 GTGAC
XhoII 678 AGATCC [GA]^GATC[CT]
2254 AGATCT
XmaCI 14 CCCGGG C^CCGGG
2099 CCCGGG
XmaI 14 CCCGGG C^CCGGG
2099 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
Alw44I AlwI ApaLI ApoI Asp700I
AspHI AvaI AvaII BalI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaHI BsaJI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
BstUI BstXI Cac8I CfoI Cfr13I
DdeI DpnII DsaI EaeI EcoNI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp509I
TspRI TthHB8I XmnI