################################## # NO CUT ENZYMES ( 121 enzymes) # ################################## Acc65I AccI AccII AccIII AclI AflII AgeI Alw44I AlwNI AocI Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AspHI AvaI AviII AvrII BbeI BbrPI BciVI BfrI BglI BglII BlnI BlpI Bpu1102I BsaAI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmBI BsmI BsoBI Bsp1407I BspDI BspEI BsrBI BsrGI BssHII BssSI Bst1107I BstAPI BstEII BstPI BstUI BstZ17I Bsu36I CelII ClaI CpoI DraI DraIII DrdI EagI EclXI Eco47III Eco52I Eco81I EcoO65I EcoRI EcoRV EcoT22I FseI FspI HaeII HgaI KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI ScaI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI SspI StuI SwaI XbaI XcmI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AatII 1129 GACGTC GACGT^C AcsI 1633 GAATTT [GA]^AATT[CT] AcyI 1129 GACGTC G[GA]^CG[CT]C AflIII 864 ACATGT A^C[GA][CT]GT AhdI 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApaI 159 GGGCCC GGGCC^C ApoI 1633 GAATTT [GA]^AATT[CT] AspEI 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BanII 159 GGGCCC G[GA]GC[CT]^C BbsI 345 GAAGAC GAAGAC(2/6) BbvCI 670 CCTCAGC CCTCAGC(-5/-2) BcgI 945 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 1672 TGATCA T^GATCA BcnI 62 CCCGG CC^[GC]GG BfaI 1420 CTAG C^TAG BpuAI 345 GAAGAC GAAGAC(2/6) BsaHI 1129 GACGTC G[GA]^CG[CT]C BsaI 1594 GGTCTC GGTCTC(1/5) BsgI 1246 CTGCAC GTGCAG(16/14) BsmFI 1127 GGGAC GGGAC(10/14) BspHI 1133 TCATGA T^CATGA BspLU11I 864 ACATGT A^CATGT BspMI 552 ACCTGC ACCTGC(4/8) BsrFI 1114 ACCGGC [GA]^CCGG[CT] BstBI 1169 TTCGAA TT^CGAA BstXI 224 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Cfr10I 1114 ACCGGC [GA]^CCGG[CT] Eam1105I 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoNI 187 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FauI 183 CCCGC CCCGC(4/6) FbaI 1672 TGATCA T^GATCA Hin1I 1129 GACGTC G[GA]^CG[CT]C HindIII 1651 AAGCTT A^AGCTT HpaI 358 GTTAAC GTT^AAC HphI 1093 GGTGA GGTGA(8/7) MaeI 1420 CTAG C^TAG MslI 1065 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NciI 62 CCCGG CC^[GC]GG NcoI 92 CCATGG C^CATGG NspI 864 ACATGT [GA]CATG^[CT] NspV 1169 TTCGAA TT^CGAA PpuMI 1475 AGGACCT [GA]G^G[AT]CC[CT] RcaI 1133 TCATGA T^CATGA SbfI 408 CCTGCAGG CCTGCA^GG SexAI 1590 ACCTGGT A^CC[AT]GGT SfaNI 877 GATGC GCATC(5/9) SfuI 1169 TTCGAA TT^CGAA SmlI 436 CTTGAG C^T[CT][GA]AG Sse8387I 408 CCTGCAGG CCTGCA^GG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AfaI 32 GTAC GT^AC 594 GTAC AspI 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1589 GACCTGGTC BalI 221 TGGCCA TGG^CCA 704 TGGCCA BamHI 417 GGATCC G^GATCC 447 GGATCC BanI 629 GGCACC G^G[CT][GA]CC 1663 GGCACC BmyI 159 GGGCCC G[AGT]GC[ACT]^C 292 GGGCAC Bsp1286I 159 GGGCCC G[AGT]GC[ACT]^C 292 GGGCAC BsrDI 1175 GCAATG GCAATG(2/0) 1715 GCAATG CfoI 430 GCGC GCG^C 1341 GCGC DraII 158 AGGGCCC [GA]G^G[ACGT]CC[CT] 1475 AGGACCT DsaI 92 CCATGG C^C[GA][CT]GG 218 CCGTGG EarI 105 GAAGAG CTCTTC(1/4) 1483 GAAGAG EcoO109I 158 AGGGCCC [GA]G^G[ACGT]CC[CT] 1475 AGGACCT HapII 63 CCGG C^CGG 1115 CCGG HhaI 430 GCGC GCG^C 1341 GCGC HinP1I 430 GCGC G^CGC 1341 GCGC HpaII 63 CCGG C^CGG 1115 CCGG Ksp632I 105 GAAGAG CTCTTC(1/4) 1483 GAAGAG MaeII 1130 ACGT A^CGT 1499 ACGT MluNI 221 TGGCCA TGG^CCA 704 TGGCCA MscI 221 TGGCCA TGG^CCA 704 TGGCCA MspI 63 CCGG C^CGG 1115 CCGG PflFI 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1589 GACCTGGTC RsaI 32 GTAC GT^AC 594 GTAC Tth111I 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC 1589 GACCTGGTC ######################################### # 3 OR MORE CUT ENZYMES ( 63 enzymes) # ######################################### AciI AluI AlwI AvaII BbvI BpmI BsaBI BsaJI BseRI BsiYI BslI BsmAI BsrI BssKI BstF5I BstNI BstYI Cac8I Cfr13I DdeI DpnII EaeI EcoRII EcoT14I Fnu4HI FokI HaeIII HincII HindII HinfI ItaI MaeIII MamI MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NlaIII NlaIV PflMI PleI PstI PvuII Sau3AI Sau96I ScrFI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII