##################################
# NO CUT ENZYMES ( 121 enzymes) #
##################################
Acc65I AccI AccII AccIII AclI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
Asp700I Asp718I AspHI AvaI AviII
AvrII BbeI BbrPI BciVI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaWI BseAI BsiEI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BsrBI BsrGI BssHII
BssSI Bst1107I BstAPI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII DrdI EagI
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI KasI KpnI KspI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SapI ScaI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI SspI StuI SwaI
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AatII 1129 GACGTC GACGT^C
AcsI 1633 GAATTT [GA]^AATT[CT]
AcyI 1129 GACGTC G[GA]^CG[CT]C
AflIII 864 ACATGT A^C[GA][CT]GT
AhdI 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 159 GGGCCC GGGCC^C
ApoI 1633 GAATTT [GA]^AATT[CT]
AspEI 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanII 159 GGGCCC G[GA]GC[CT]^C
BbsI 345 GAAGAC GAAGAC(2/6)
BbvCI 670 CCTCAGC CCTCAGC(-5/-2)
BcgI 945 CGACTCCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1672 TGATCA T^GATCA
BcnI 62 CCCGG CC^[GC]GG
BfaI 1420 CTAG C^TAG
BpuAI 345 GAAGAC GAAGAC(2/6)
BsaHI 1129 GACGTC G[GA]^CG[CT]C
BsaI 1594 GGTCTC GGTCTC(1/5)
BsgI 1246 CTGCAC GTGCAG(16/14)
BsmFI 1127 GGGAC GGGAC(10/14)
BspHI 1133 TCATGA T^CATGA
BspLU11I 864 ACATGT A^CATGT
BspMI 552 ACCTGC ACCTGC(4/8)
BsrFI 1114 ACCGGC [GA]^CCGG[CT]
BstBI 1169 TTCGAA TT^CGAA
BstXI 224 CCAAGACTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 1114 ACCGGC [GA]^CCGG[CT]
Eam1105I 228 GACTCTGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 187 CCTGGAGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FauI 183 CCCGC CCCGC(4/6)
FbaI 1672 TGATCA T^GATCA
Hin1I 1129 GACGTC G[GA]^CG[CT]C
HindIII 1651 AAGCTT A^AGCTT
HpaI 358 GTTAAC GTT^AAC
HphI 1093 GGTGA GGTGA(8/7)
MaeI 1420 CTAG C^TAG
MslI 1065 CATGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NciI 62 CCCGG CC^[GC]GG
NcoI 92 CCATGG C^CATGG
NspI 864 ACATGT [GA]CATG^[CT]
NspV 1169 TTCGAA TT^CGAA
PpuMI 1475 AGGACCT [GA]G^G[AT]CC[CT]
RcaI 1133 TCATGA T^CATGA
SbfI 408 CCTGCAGG CCTGCA^GG
SexAI 1590 ACCTGGT A^CC[AT]GGT
SfaNI 877 GATGC GCATC(5/9)
SfuI 1169 TTCGAA TT^CGAA
SmlI 436 CTTGAG C^T[CT][GA]AG
Sse8387I 408 CCTGCAGG CCTGCA^GG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AfaI 32 GTAC GT^AC
594 GTAC
AspI 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1589 GACCTGGTC
BalI 221 TGGCCA TGG^CCA
704 TGGCCA
BamHI 417 GGATCC G^GATCC
447 GGATCC
BanI 629 GGCACC G^G[CT][GA]CC
1663 GGCACC
BmyI 159 GGGCCC G[AGT]GC[ACT]^C
292 GGGCAC
Bsp1286I 159 GGGCCC G[AGT]GC[ACT]^C
292 GGGCAC
BsrDI 1175 GCAATG GCAATG(2/0)
1715 GCAATG
CfoI 430 GCGC GCG^C
1341 GCGC
DraII 158 AGGGCCC [GA]G^G[ACGT]CC[CT]
1475 AGGACCT
DsaI 92 CCATGG C^C[GA][CT]GG
218 CCGTGG
EarI 105 GAAGAG CTCTTC(1/4)
1483 GAAGAG
EcoO109I 158 AGGGCCC [GA]G^G[ACGT]CC[CT]
1475 AGGACCT
HapII 63 CCGG C^CGG
1115 CCGG
HhaI 430 GCGC GCG^C
1341 GCGC
HinP1I 430 GCGC G^CGC
1341 GCGC
HpaII 63 CCGG C^CGG
1115 CCGG
Ksp632I 105 GAAGAG CTCTTC(1/4)
1483 GAAGAG
MaeII 1130 ACGT A^CGT
1499 ACGT
MluNI 221 TGGCCA TGG^CCA
704 TGGCCA
MscI 221 TGGCCA TGG^CCA
704 TGGCCA
MspI 63 CCGG C^CGG
1115 CCGG
PflFI 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1589 GACCTGGTC
RsaI 32 GTAC GT^AC
594 GTAC
Tth111I 1411 GACAAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
1589 GACCTGGTC
#########################################
# 3 OR MORE CUT ENZYMES ( 63 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BpmI BsaBI BsaJI BseRI BsiYI
BslI BsmAI BsrI BssKI BstF5I
BstNI BstYI Cac8I Cfr13I DdeI
DpnII EaeI EcoRII EcoT14I Fnu4HI
FokI HaeIII HincII HindII HinfI
ItaI MaeIII MamI MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV PflMI
PleI PstI PvuII Sau3AI Sau96I
ScrFI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XhoII