##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI AspEI AspI AvrII
BalI BamHI BbeI BbvCI BcgI
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BsiWI BsmI BspDI
BspHI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI Cfr10I ClaI
DraIII EagI Eam1105I EclXI Eco47III
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FseI HindIII HpaI KasI MamI
MfeI MluNI MscI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PflFI PinAI PmeI PshBI Psp1406I
PvuI PvuII RcaI SacI SalI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspI SwaI Tth111I XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
Acc65I 517 GGTACC G^GTACC
AccIII 1720 TCCGGA T^CCGGA
AcyI 51 GACGCC G[GA]^CG[CT]C
AlwNI 429 CAGGCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 1281 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 517 GGTACC G^GTACC
AvaI 1360 CTCGAG C^[CT]CG[GA]G
AviII 1222 TGCGCA TGC^GCA
BanII 473 GGGCTC G[GA]GC[CT]^C
BbrPI 357 CACGTG CAC^GTG
BclI 644 TGATCA T^GATCA
BlpI 91 GCTCAGC GC^T[ACGT]AGC
BpmI 1408 CTGGAG CTGGAG(16/14)
Bpu1102I 91 GCTCAGC GC^T[ACGT]AGC
BsaHI 51 GACGCC G[GA]^CG[CT]C
BseAI 1720 TCCGGA T^CCGGA
BsiEI 1379 CGACCG CG[GA][CT]^CG
BsmBI 1826 CGTCTC CGTCTC(1/5)
BsoBI 1360 CTCGAG C^[CT]CG[GA]G
Bsp1407I 1633 TGTACA T^GTACA
BspEI 1720 TCCGGA T^CCGGA
BsrBI 89 CCGCTC CCGCTC(-3/-3)
BsrGI 1633 TGTACA T^GTACA
Bst1107I 1785 GTATAC GTA^TAC
BstZ17I 1785 GTATAC GTA^TAC
CelII 91 GCTCAGC GC^T[ACGT]AGC
CpoI 1330 CGGTCCG CG^G[AT]CCG
DraI 2063 TTTAAA TTT^AAA
DrdI 1693 GACCCCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 1805 CGGCCA [CT]^GGCC[GA]
EcoNI 1050 CCTCAGAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 1509 CCAAGG C^C[AT][AT]GG
FbaI 644 TGATCA T^GATCA
FspI 1222 TGCGCA TGC^GCA
HaeII 1952 GGCGCT [GA]GCGC^[CT]
Hin1I 51 GACGCC G[GA]^CG[CT]C
KpnI 517 GGTACC GGTAC^C
MluI 26 ACGCGT A^CGCGT
MroI 1720 TCCGGA T^CCGGA
MslI 357 CACGTGGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
PaeR7I 1360 CTCGAG C^TCGAG
PmaCI 357 CACGTG CAC^GTG
PmlI 357 CACGTG CAC^GTG
PshAI 452 GACCTTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1563 CTGCAG CTGCA^G
RsrII 1330 CGGTCCG CG^G[AT]CCG
ScaI 175 AGTACT AGT^ACT
SspBI 1633 TGTACA T^GTACA
StuI 1002 AGGCCT AGG^CCT
StyI 1509 CCAAGG C^C[AT][AT]GG
XbaI 2090 TCTAGA T^CTAGA
XhoI 1360 CTCGAG C^TCGAG
XmnI 1281 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 285 GTCTAC GT^[AC][GT]AC
1785 GTATAC
AlwI 350 GATCC GGATC(4/5)
989 GATCC
AocI 468 CCTGAGG CC^T[ACGT]AGG
762 CCTGAGG
AspHI 806 GAGCAC G[AT]GC[AT]^C
1667 GTGCTC
BfaI 139 CTAG C^TAG
2091 CTAG
BsaAI 69 CACGTA [CT]AC^GT[GA]
357 CACGTG
BsaWI 704 ACCGGA [AT]^CCGG[AT]
1720 TCCGGA
BsgI 798 GTGCAG GTGCAG(16/14)
1467 GTGCAG
BsiHKAI 806 GAGCAC G[AT]GC[AT]^C
1667 GTGCTC
BspLU11I 205 ACATGT A^CATGT
2016 ACATGT
BspMI 1153 ACCTGC ACCTGC(4/8)
101 GCAGGT
BsrDI 658 GCAATG GCAATG(2/0)
1207 GCAATG
BstXI 115 CCAAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1196 CCAAAATTTTGG
BstYI 349 AGATCC [GA]^GATC[CT]
988 AGATCC
Bsu36I 468 CCTGAGG CC^T[ACGT]AGG
762 CCTGAGG
Eco81I 468 CCTGAGG CC^T[ACGT]AGG
762 CCTGAGG
HincII 1167 GTTGAC GT[CT]^[GA]AC
2114 GTTGAC
HindII 1167 GTTGAC GT[CT]^[GA]AC
2114 GTTGAC
KspI 33 CCGCGG CCGC^GG
1454 CCGCGG
MaeI 139 CTAG C^TAG
2091 CTAG
MflI 349 AGATCC [GA]^GATC[CT]
988 AGATCC
MseI 1574 TTAA T^TAA
2064 TTAA
NspI 205 ACATGT [GA]CATG^[CT]
2016 ACATGT
SacII 33 CCGCGG CCGC^GG
1454 CCGCGG
SapI 803 GAAGAGC GCTCTTC(1/4)
1139 GCTCTTC
SfcI 688 CTGTAG C^T[GA][CT]AG
1563 CTGCAG
Tru9I 1574 TTAA T^TAA
2064 TTAA
XcmI 112 CCACCAAAGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1136 CCAGCTCTTCATTGG
XhoII 349 AGATCC [GA]^GATC[CT]
988 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 80 enzymes) #
#########################################
AccII AciI AcsI AfaI AflIII
AluI ApoI AvaII BanI BbsI
BbvI BcnI BmyI BpuAI BsaJI
BseRI BsiYI BslI BsmAI BsmFI
Bsp1286I BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeII
MaeIII MboI MboII MnlI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PflMI PleI
PpuMI RsaI Sau3AI Sau96I ScrFI
SfaNI SmlI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I