##################################
# NO CUT ENZYMES ( 97 enzymes) #
##################################
Acc65I AccIII AclI AflIII AgeI
AhdI AlwNI Aor51HI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BcgI BlnI
BsaBI BsaWI BseAI BsgI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
BssSI BstBI BstXI ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I EcoNI EcoRV
FseI FspI HgaI HindIII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NheI NotI NspV
PacI PflFI PflMI PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI Tth111I Van91I XbaI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AccII 346 CGCG CG^CG
AcsI 307 GAATTC [GA]^AATT[CT]
AflII 1937 CTTAAG C^TTAAG
Alw44I 1293 GTGCAC G^TGCAC
AlwI 614 GGATC GGATC(4/5)
ApaI 1880 GGGCCC GGGCC^C
ApaLI 1293 GTGCAC G^TGCAC
ApoI 307 GAATTC [GA]^AATT[CT]
AvaI 108 CTCGAG C^[CT]CG[GA]G
BalI 873 TGGCCA TGG^CCA
BbrPI 1602 CACGTG CAC^GTG
BclI 97 TGATCA T^GATCA
BfrI 1937 CTTAAG C^TTAAG
BlpI 2279 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2279 GCTGAGC GC^T[ACGT]AGC
BsoBI 108 CTCGAG C^[CT]CG[GA]G
BsrBI 148 CCGCTC CCGCTC(-3/-3)
BsrDI 1584 CATTGC GCAATG(2/0)
Bst1107I 2445 GTATAC GTA^TAC
BstEII 638 GGTGACC G^GT[ACGT]ACC
BstPI 638 GGTGACC G^GT[ACGT]ACC
BstUI 346 CGCG CG^CG
BstZ17I 2445 GTATAC GTA^TAC
CelII 2279 GCTGAGC GC^T[ACGT]AGC
EaeI 873 TGGCCA [CT]^GGCC[GA]
EcoO65I 638 GGTGACC G^GT[ACGT]ACC
EcoRI 307 GAATTC G^AATTC
EcoT22I 2525 ATGCAT ATGCA^T
FbaI 97 TGATCA T^GATCA
MluNI 873 TGGCCA TGG^CCA
MscI 873 TGGCCA TGG^CCA
MspA1I 1360 CAGCTG C[AC]G^C[GT]G
MvnI 346 CGCG CG^CG
NaeI 33 GCCGGC GCC^GGC
NdeI 1536 CATATG CA^TATG
NgoMIV 33 GCCGGC G^CCGGC
NruI 345 TCGCGA TCG^CGA
NsiI 2525 ATGCAT ATGCA^T
PaeR7I 108 CTCGAG C^TCGAG
PmaCI 1602 CACGTG CAC^GTG
PmlI 1602 CACGTG CAC^GTG
PpuMI 137 GGGACCT [GA]G^G[AT]CC[CT]
PshAI 492 GACCTGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 1360 CAGCTG CAG^CTG
SapI 2174 GCTCTTC GCTCTTC(1/4)
TaqI 109 TCGA T^CGA
TfiI 1161 GAATC G^A[AT]TC
TthHB8I 109 TCGA T^CGA
XhoI 108 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AatII 781 GACGTC GACGT^C
1489 GACGTC
BanI 446 GGCGCC G^G[CT][GA]CC
1338 GGCGCC
BanII 1880 GGGCCC G[GA]GC[CT]^C
2189 GGGCTC
BbeI 446 GGCGCC GGCGC^C
1338 GGCGCC
BbvCI 401 CCTCAGC CCTCAGC(-5/-2)
1357 CCTCAGC
BciVI 2208 GGATAC GTATCC(6/5)
1043 GTATCC
BglI 33 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1599 GCCCACGTGGC
BglII 72 AGATCT A^GATCT
1728 AGATCT
BsaAI 528 CACGTA [CT]AC^GT[GA]
1602 CACGTG
BsrFI 33 GCCGGC [GA]^CCGG[CT]
1791 ACCGGC
BstAPI 458 GCAAATGTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1775 GCATCTTATGC
BstYI 72 AGATCT [GA]^GATC[CT]
1728 AGATCT
Cfr10I 33 GCCGGC [GA]^CCGG[CT]
1791 ACCGGC
DraII 137 GGGACCT [GA]G^G[ACGT]CC[CT]
1880 GGGCCCT
DsaI 235 CCATGG C^C[GA][CT]GG
1126 CCATGG
EcoO109I 137 GGGACCT [GA]G^G[ACGT]CC[CT]
1880 GGGCCCT
FauI 839 GCGGG CCCGC(4/6)
2167 GCGGG
HincII 1703 GTTGAC GT[CT]^[GA]AC
1898 GTCAAC
HindII 1703 GTTGAC GT[CT]^[GA]AC
1898 GTCAAC
KasI 446 GGCGCC G^GCGCC
1338 GGCGCC
MflI 72 AGATCT [GA]^GATC[CT]
1728 AGATCT
MslI 2394 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2484 CACATACATG
NarI 446 GGCGCC GG^CGCC
1338 GGCGCC
NcoI 235 CCATGG C^CATGG
1126 CCATGG
NspI 1395 GCATGT [GA]CATG^[CT]
2489 ACATGC
PstI 68 CTGCAG CTGCA^G
164 CTGCAG
ScaI 668 AGTACT AGT^ACT
2226 AGTACT
SfcI 68 CTGCAG C^T[GA][CT]AG
164 CTGCAG
SfoI 446 GGCGCC GGC^GCC
1338 GGCGCC
SmlI 108 CTCGAG C^T[CT][GA]AG
1937 CTTAAG
XhoII 72 AGATCT [GA]^GATC[CT]
1728 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 81 enzymes) #
#########################################
AccI AciI AcyI AfaI AluI
AocI AspHI AvaII BbsI BbvI
BcnI BfaI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Bsu36I Cac8I CfoI Cfr13I DdeI
DpnII EarI Eco81I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PleI RsaI Sau3AI
Sau96I ScrFI SfaNI StuI StyI
Tru9I TseI Tsp45I Tsp509I TspRI
XcmI