##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AspEI AspI AvaI AviII
BanII BbeI BcgI BciVI BclI
BcnI BfrI BglI BglII BlpI
Bpu1102I BsaBI BsaI BsaWI BseAI
BsgI BsiEI BsiWI BsmBI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII BssSI BstAPI
BstBI BstEII BstPI BstXI Bsu36I
CelII Cfr10I ClaI CpoI DrdI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV FbaI
FseI FspI HaeII HapII HpaI
HpaII KasI KspI MamI MfeI
MluI MroI MslI MspI MunI
NaeI NarI NciI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
Psp1406I PstI PvuI RsrII SacI
SacII SalI SbfI SfiI SfoI
SfuI SgrAI SmaI SnaBI Sse8387I
StuI SwaI Tth111I XcmI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 1233 GACGTC GACGT^C
Acc65I 1951 GGTACC G^GTACC
AccII 261 CGCG CG^CG
AcyI 1233 GACGTC G[GA]^CG[CT]C
AflIII 520 ACACGT A^C[GA][CT]GT
AseI 1155 ATTAAT AT^TAAT
AsnI 1155 ATTAAT AT^TAAT
Asp718I 1951 GGTACC G^GTACC
AvrII 1040 CCTAGG C^CTAGG
BalI 1423 TGGCCA TGG^CCA
BanI 1951 GGTACC G^G[CT][GA]CC
BbvCI 581 CCTCAGC CCTCAGC(-5/-2)
BlnI 1040 CCTAGG C^CTAGG
BsaHI 1233 GACGTC G[GA]^CG[CT]C
Bsp1407I 690 TGTACA T^GTACA
BspHI 1873 TCATGA T^CATGA
BsrGI 690 TGTACA T^GTACA
Bst1107I 749 GTATAC GTA^TAC
BstUI 261 CGCG CG^CG
BstZ17I 749 GTATAC GTA^TAC
DraIII 61 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
EaeI 1423 TGGCCA [CT]^GGCC[GA]
EcoT22I 948 ATGCAT ATGCA^T
FauI 1944 CCCGC CCCGC(4/6)
HgaI 1373 GACGC GACGC(5/10)
Hin1I 1233 GACGTC G[GA]^CG[CT]C
HincII 1402 GTCAAC GT[CT]^[GA]AC
HindII 1402 GTCAAC GT[CT]^[GA]AC
KpnI 1951 GGTACC GGTAC^C
MluNI 1423 TGGCCA TGG^CCA
MscI 1423 TGGCCA TGG^CCA
MvnI 261 CGCG CG^CG
NcoI 985 CCATGG C^CATGG
NsiI 948 ATGCAT ATGCA^T
PpuMI 989 GGGTCCC [GA]G^G[AT]CC[CT]
PshBI 1155 ATTAAT AT^TAAT
RcaI 1873 TCATGA T^CATGA
SapI 652 GAAGAGC GCTCTTC(1/4)
SexAI 62 ACCTGGT A^CC[AT]GGT
SpeI 1765 ACTAGT A^CTAGT
SphI 420 GCATGC GCATG^C
SspBI 690 TGTACA T^GTACA
SspI 814 AATATT AAT^ATT
TaqI 885 TCGA T^CGA
TthHB8I 885 TCGA T^CGA
XbaI 1860 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AlwNI 1467 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1932 CAGGGCCTG
Asp700I 1523 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1809 GAAGTCTTTC
AvaII 219 GGACC G^G[AT]CC
990 GGTCC
BbrPI 406 CACGTG CAC^GTG
521 CACGTG
BpmI 314 CTCCAG CTGGAG(16/14)
350 CTCCAG
BsaAI 406 CACGTG [CT]AC^GT[GA]
521 CACGTG
BseRI 55 CTCCTC GAGGAG(10/8)
355 GAGGAG
BsmFI 388 GGGAC GGGAC(10/14)
991 GTCCC
BsrDI 481 GCAATG GCAATG(2/0)
1001 GCAATG
CfoI 262 GCGC GCG^C
532 GCGC
DraI 1129 TTTAAA TTT^AAA
2086 TTTAAA
DsaI 540 CCGTGG C^C[GA][CT]GG
985 CCATGG
EarI 1869 CTCTTC CTCTTC(1/4)
652 GAAGAG
HhaI 262 GCGC GCG^C
532 GCGC
HinP1I 262 GCGC G^CGC
532 GCGC
HindIII 906 AAGCTT A^AGCTT
1820 AAGCTT
Ksp632I 1869 CTCTTC CTCTTC(1/4)
652 GAAGAG
PflMI 96 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
454 CCAACACCTGG
PleI 1381 GACTC GAGTC(4/5)
1535 GAGTC
PmaCI 406 CACGTG CAC^GTG
521 CACGTG
PmlI 406 CACGTG CAC^GTG
521 CACGTG
PvuII 434 CAGCTG CAG^CTG
1840 CAGCTG
ScaI 332 AGTACT AGT^ACT
1124 AGTACT
SfcI 1449 CTACAG C^T[GA][CT]AG
1692 CTGTAG
TfiI 21 GAATC G^A[AT]TC
1643 GAATC
Van91I 96 CCACATGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
454 CCAACACCTGG
XmnI 1523 GAAAGTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1809 GAAGTCTTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AccI AciI AcsI AfaI AluI
AlwI ApoI AspHI BamHI BbsI
BbvI BfaI BmyI BpuAI BsaJI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EcoO109I EcoRI EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI HphI ItaI
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MspA1I MvaI
MwoI NdeII NlaIII NlaIV NspI
RsaI Sau3AI Sau96I ScrFI SfaNI
SmlI StyI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII