##################################
# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp718I AspEI AspHI AviII
AvrII BalI BamHI BanII BbeI
BbrPI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaBI
BsaHI BsaI BsaWI BseAI BseRI
BsiHKAI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeII HapII HgaI Hin1I
HpaII KasI KpnI KspI MamI
MfeI MluI MluNI MroI MscI
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI Psp1406I RcaI RsrII SacI
SacII SalI SapI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI SspBI StuI StyI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 32 enzymes) #
###################################
AlwI 1355 GATCC GGATC(4/5)
AspI 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 251 CTCGGG C^[CT]CG[GA]G
BanI 1998 GGCACC G^G[CT][GA]CC
BcgI 437 GCACTACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 2340 GGATAC GTATCC(6/5)
BmyI 831 GTGCCC G[AGT]GC[ACT]^C
BsaAI 506 TACGTG [CT]AC^GT[GA]
BsiEI 443 CGATCG CG[GA][CT]^CG
BsoBI 251 CTCGGG C^[CT]CG[GA]G
Bsp1286I 831 GTGCCC G[AGT]GC[ACT]^C
BsrBI 243 GAGCGG CCGCTC(-3/-3)
BstAPI 1206 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1098 TTCGAA TT^CGAA
BstYI 1354 AGATCC [GA]^GATC[CT]
CfoI 1061 GCGC GCG^C
HhaI 1061 GCGC GCG^C
HinP1I 1061 GCGC G^CGC
HindIII 520 AAGCTT A^AGCTT
HpaI 2146 GTTAAC GTT^AAC
MflI 1354 AGATCC [GA]^GATC[CT]
NspV 1098 TTCGAA TT^CGAA
PflFI 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 1204 CTGCAG CTGCA^G
PvuI 443 CGATCG CGAT^CG
PvuII 274 CAGCTG CAG^CTG
SbfI 1203 CCTGCAGG CCTGCA^GG
ScaI 2012 AGTACT AGT^ACT
SfuI 1098 TTCGAA TT^CGAA
Sse8387I 1203 CCTGCAGG CCTGCA^GG
Tth111I 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1354 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AlwNI 229 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
1112 CAGGCTCTG
AseI 589 ATTAAT AT^TAAT
2131 ATTAAT
AsnI 589 ATTAAT AT^TAAT
2131 ATTAAT
Asp700I 349 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
579 GAATTCCTTC
BbvCI 15 GCTGAGG CCTCAGC(-5/-2)
1398 GCTGAGG
BsgI 1499 CTGCAC GTGCAG(16/14)
271 GTGCAG
BspMI 1202 ACCTGC ACCTGC(4/8)
1619 ACCTGC
BstXI 1057 CCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1568 CCACTATGCTGG
DraI 898 TTTAAA TTT^AAA
1086 TTTAAA
FauI 1262 GCGGG CCCGC(4/6)
1366 GCGGG
HincII 659 GTCAAC GT[CT]^[GA]AC
2146 GTTAAC
HindII 659 GTCAAC GT[CT]^[GA]AC
2146 GTTAAC
MspA1I 274 CAGCTG C[AC]G^C[GT]G
1364 CAGCGG
MwoI 178 GCCTTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
1206 GCAGGCTTTGC
PshBI 589 ATTAAT AT^TAAT
2131 ATTAAT
SmlI 151 CTCAAG C^T[CT][GA]AG
964 CTTGAG
XmnI 349 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
579 GAATTCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
ApoI AvaII BbsI BbvI BfaI
BpmI BpuAI BsaJI BsiYI BslI
BsmAI BsmFI BspLU11I BsrI BssKI
BstF5I BstNI Cac8I Cfr13I DdeI
DpnII DraII EarI EcoO109I EcoRI
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MnlI MseI
MslI MvaI NdeII NlaIII NlaIV
NspI PleI PpuMI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I