################################## # NO CUT ENZYMES ( 141 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AocI Aor51HI ApaI ApaLI AscI Asp718I AspEI AspHI AviII AvrII BalI BamHI BanII BbeI BbrPI BclI BcnI BfrI BglI BglII BlnI BlpI Bpu1102I BsaBI BsaHI BsaI BsaWI BseAI BseRI BsiHKAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BsrDI BsrFI BsrGI BssHII BssSI Bst1107I BstEII BstPI BstUI BstZ17I Bsu36I CelII Cfr10I ClaI CpoI DraIII DrdI DsaI EaeI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT14I EcoT22I FbaI FseI FspI HaeII HapII HgaI Hin1I HpaII KasI KpnI KspI MamI MfeI MluI MluNI MroI MscI MspI MunI MvnI NaeI NarI NciI NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI Psp1406I RcaI RsrII SacI SacII SalI SapI SexAI SfiI SfoI SgrAI SmaI SnaBI SpeI SphI SspBI StuI StyI SwaI Van91I XbaI XcmI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 32 enzymes) # ################################### AlwI 1355 GATCC GGATC(4/5) AspI 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 251 CTCGGG C^[CT]CG[GA]G BanI 1998 GGCACC G^G[CT][GA]CC BcgI 437 GCACTACGATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 2340 GGATAC GTATCC(6/5) BmyI 831 GTGCCC G[AGT]GC[ACT]^C BsaAI 506 TACGTG [CT]AC^GT[GA] BsiEI 443 CGATCG CG[GA][CT]^CG BsoBI 251 CTCGGG C^[CT]CG[GA]G Bsp1286I 831 GTGCCC G[AGT]GC[ACT]^C BsrBI 243 GAGCGG CCGCTC(-3/-3) BstAPI 1206 GCAGGCTTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstBI 1098 TTCGAA TT^CGAA BstYI 1354 AGATCC [GA]^GATC[CT] CfoI 1061 GCGC GCG^C HhaI 1061 GCGC GCG^C HinP1I 1061 GCGC G^CGC HindIII 520 AAGCTT A^AGCTT HpaI 2146 GTTAAC GTT^AAC MflI 1354 AGATCC [GA]^GATC[CT] NspV 1098 TTCGAA TT^CGAA PflFI 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 1204 CTGCAG CTGCA^G PvuI 443 CGATCG CGAT^CG PvuII 274 CAGCTG CAG^CTG SbfI 1203 CCTGCAGG CCTGCA^GG ScaI 2012 AGTACT AGT^ACT SfuI 1098 TTCGAA TT^CGAA Sse8387I 1203 CCTGCAGG CCTGCA^GG Tth111I 1405 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XhoII 1354 AGATCC [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AlwNI 229 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG 1112 CAGGCTCTG AseI 589 ATTAAT AT^TAAT 2131 ATTAAT AsnI 589 ATTAAT AT^TAAT 2131 ATTAAT Asp700I 349 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 579 GAATTCCTTC BbvCI 15 GCTGAGG CCTCAGC(-5/-2) 1398 GCTGAGG BsgI 1499 CTGCAC GTGCAG(16/14) 271 GTGCAG BspMI 1202 ACCTGC ACCTGC(4/8) 1619 ACCTGC BstXI 1057 CCATGCGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1568 CCACTATGCTGG DraI 898 TTTAAA TTT^AAA 1086 TTTAAA FauI 1262 GCGGG CCCGC(4/6) 1366 GCGGG HincII 659 GTCAAC GT[CT]^[GA]AC 2146 GTTAAC HindII 659 GTCAAC GT[CT]^[GA]AC 2146 GTTAAC MspA1I 274 CAGCTG C[AC]G^C[GT]G 1364 CAGCGG MwoI 178 GCCTTTGATGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 1206 GCAGGCTTTGC PshBI 589 ATTAAT AT^TAAT 2131 ATTAAT SmlI 151 CTCAAG C^T[CT][GA]AG 964 CTTGAG XmnI 349 GAATCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 579 GAATTCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 68 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI ApoI AvaII BbsI BbvI BfaI BpmI BpuAI BsaJI BsiYI BslI BsmAI BsmFI BspLU11I BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EarI EcoO109I EcoRI EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MnlI MseI MslI MvaI NdeII NlaIII NlaIV NspI PleI PpuMI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SspI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I