##################################
# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccIII AclI AflII AgeI
AhdI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI AspEI AspHI
AviII AvrII BbeI BbrPI BbvCI
BfrI BglII BlnI BlpI Bpu1102I
BseAI BsiEI BsiHKAI BsiWI BsmI
Bsp1407I BspEI BsrFI BsrGI BssHII
BssSI BstBI BstEII BstPI Bsu36I
CelII Cfr10I CpoI DraIII EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO65I EcoRV EcoT22I FseI
FspI HaeII HincII HindII HpaI
KasI KspI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NotI NruI NsiI NspV PacI
PaeR7I PflMI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 47 enzymes) #
###################################
AccI 1693 GTATAC GT^[AC][GT]AC
AcyI 1020 GGCGTC G[GA]^CG[CT]C
AflIII 1821 ACATGT A^C[GA][CT]GT
ApaI 1180 GGGCCC GGGCC^C
AspI 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 195 CTCGGG C^[CT]CG[GA]G
BalI 1611 TGGCCA TGG^CCA
BamHI 1097 GGATCC G^GATCC
BanII 1180 GGGCCC G[GA]GC[CT]^C
BclI 751 TGATCA T^GATCA
BcnI 4 CCCGG CC^[GC]GG
BglI 345 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 1541 CACGTA [CT]AC^GT[GA]
BsaHI 1020 GGCGTC G[GA]^CG[CT]C
BsaWI 72 TCCGGT [AT]^CCGG[AT]
BseRI 1805 CTCCTC GAGGAG(10/8)
BsoBI 195 CTCGGG C^[CT]CG[GA]G
BspDI 1263 ATCGAT AT^CGAT
BspLU11I 1821 ACATGT A^CATGT
BspMI 1660 GCAGGT ACCTGC(4/8)
Bst1107I 1693 GTATAC GTA^TAC
BstAPI 841 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 1629 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I 1693 GTATAC GTA^TAC
ClaI 1263 ATCGAT AT^CGAT
DraI 1937 TTTAAA TTT^AAA
DrdI 632 GACGGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 1930 CCATGG C^C[GA][CT]GG
EaeI 1611 TGGCCA [CT]^GGCC[GA]
EarI 1131 CTCTTC CTCTTC(1/4)
EcoRI 1670 GAATTC G^AATTC
EcoT14I 1930 CCATGG C^C[AT][AT]GG
FauI 482 GCGGG CCCGC(4/6)
FbaI 751 TGATCA T^GATCA
Hin1I 1020 GGCGTC G[GA]^CG[CT]C
Ksp632I 1131 CTCTTC CTCTTC(1/4)
MluNI 1611 TGGCCA TGG^CCA
MscI 1611 TGGCCA TGG^CCA
NciI 4 CCCGG CC^[GC]GG
NcoI 1930 CCATGG C^CATGG
NheI 1510 GCTAGC G^CTAGC
NspI 1821 ACATGT [GA]CATG^[CT]
PflFI 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 319 AGGACCC [GA]G^G[AT]CC[CT]
PstI 1198 CTGCAG CTGCA^G
StyI 1930 CCATGG C^C[AT][AT]GG
Tth111I 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
###################################
# TWO CUT ENZYMES ( 34 enzymes) #
###################################
AccII 1069 CGCG CG^CG
1280 CGCG
AlwNI 463 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1578 CAGTGCCTG
Asp700I 172 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
856 GAAACCGTTC
AvaII 31 GGTCC G^G[AT]CC
320 GGACC
BbsI 758 GAAGAC GAAGAC(2/6)
1910 GTCTTC
BcgI 2023 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
445 GCAAAAAAGTCG
BciVI 1753 GGATAC GTATCC(6/5)
69 GTATCC
BfaI 1094 CTAG C^TAG
1511 CTAG
BpuAI 758 GAAGAC GAAGAC(2/6)
1910 GTCTTC
BsaBI 1098 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1550 GATCTGCATC
BsaI 1789 GAGACC GGTCTC(1/5)
1392 GGTCTC
BsmBI 1022 CGTCTC CGTCTC(1/5)
1245 CGTCTC
BsmFI 678 GGGAC GGGAC(10/14)
692 GGGAC
BsrBI 1605 CCGCTC CCGCTC(-3/-3)
1708 CCGCTC
BstUI 1069 CGCG CG^CG
1280 CGCG
BstYI 1097 GGATCC [GA]^GATC[CT]
1975 GGATCT
CfoI 1070 GCGC GCG^C
1327 GCGC
HapII 5 CCGG C^CGG
73 CCGG
HgaI 1021 GCGTC GACGC(5/10)
1244 GCGTC
HhaI 1070 GCGC GCG^C
1327 GCGC
HinP1I 1070 GCGC G^CGC
1327 GCGC
HindIII 1423 AAGCTT A^AGCTT
1565 AAGCTT
HpaII 5 CCGG C^CGG
73 CCGG
MaeI 1094 CTAG C^TAG
1511 CTAG
MamI 1098 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1550 GATCTGCATC
MflI 1097 GGATCC [GA]^GATC[CT]
1975 GGATCT
MspI 5 CCGG C^CGG
73 CCGG
MvnI 1069 CGCG CG^CG
1280 CGCG
MwoI 345 GCCCCAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
841 GCAGTGGGTGC
SexAI 939 ACCTGGT A^CC[AT]GGT
1635 ACCTGGT
TaqI 37 TCGA T^CGA
1264 TCGA
TthHB8I 37 TCGA T^CGA
1264 TCGA
XhoII 1097 GGATCC [GA]^GATC[CT]
1975 GGATCT
XmnI 172 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
856 GAAACCGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 67 enzymes) #
#########################################
Acc65I AciI AcsI AfaI AluI
AlwI ApoI Asp718I BanI BbvI
BmyI BpmI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BspHI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI KpnI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MvaI NdeII NlaIII NlaIV
PleI PvuII RcaI RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI