##################################
# NO CUT ENZYMES (  110 enzymes) #
##################################

AatII       AccIII      AclI        AflII       AgeI        
AhdI        Alw44I      AocI        Aor51HI     ApaLI       
AscI        AseI        AsnI        AspEI       AspHI       
AviII       AvrII       BbeI        BbrPI       BbvCI       
BfrI        BglII       BlnI        BlpI        Bpu1102I    
BseAI       BsiEI       BsiHKAI     BsiWI       BsmI        
Bsp1407I    BspEI       BsrFI       BsrGI       BssHII      
BssSI       BstBI       BstEII      BstPI       Bsu36I      
CelII       Cfr10I      CpoI        DraIII      EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO65I     EcoRV       EcoT22I     FseI        
FspI        HaeII       HincII      HindII      HpaI        
KasI        KspI        MfeI        MluI        MroI        
MunI        NaeI        NarI        NdeI        NgoMIV      
NotI        NruI        NsiI        NspV        PacI        
PaeR7I      PflMI       PinAI       PmaCI       PmeI        
PmlI        PshAI       PshBI       Psp1406I    PvuI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        SfiI        SfoI        SfuI        
SgrAI       SmaI        SnaBI       SpeI        SphI        
Sse8387I    SspBI       SspI        StuI        SwaI        
Van91I      XbaI        XhoI        XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   47 enzymes) #
###################################

AccI         1693  GTATAC               GT^[AC][GT]AC
AcyI         1020  GGCGTC               G[GA]^CG[CT]C
AflIII       1821  ACATGT               A^C[GA][CT]GT
ApaI         1180  GGGCCC               GGGCC^C
AspI          589  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvaI          195  CTCGGG               C^[CT]CG[GA]G
BalI         1611  TGGCCA               TGG^CCA
BamHI        1097  GGATCC               G^GATCC
BanII        1180  GGGCCC               G[GA]GC[CT]^C
BclI          751  TGATCA               T^GATCA
BcnI            4  CCCGG                CC^[GC]GG
BglI          345  GCCCCAGTGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI        1541  CACGTA               [CT]AC^GT[GA]
BsaHI        1020  GGCGTC               G[GA]^CG[CT]C
BsaWI          72  TCCGGT               [AT]^CCGG[AT]
BseRI        1805  CTCCTC               GAGGAG(10/8)
BsoBI         195  CTCGGG               C^[CT]CG[GA]G
BspDI        1263  ATCGAT               AT^CGAT
BspLU11I     1821  ACATGT               A^CATGT
BspMI        1660  GCAGGT               ACCTGC(4/8)
Bst1107I     1693  GTATAC               GTA^TAC
BstAPI        841  GCAGTGGGTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        1629  CCAACAACCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstZ17I      1693  GTATAC               GTA^TAC
ClaI         1263  ATCGAT               AT^CGAT
DraI         1937  TTTAAA               TTT^AAA
DrdI          632  GACGGCACTGTC         GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI         1930  CCATGG               C^C[GA][CT]GG
EaeI         1611  TGGCCA               [CT]^GGCC[GA]
EarI         1131  CTCTTC               CTCTTC(1/4)
EcoRI        1670  GAATTC               G^AATTC
EcoT14I      1930  CCATGG               C^C[AT][AT]GG
FauI          482  GCGGG                CCCGC(4/6)
FbaI          751  TGATCA               T^GATCA
Hin1I        1020  GGCGTC               G[GA]^CG[CT]C
Ksp632I      1131  CTCTTC               CTCTTC(1/4)
MluNI        1611  TGGCCA               TGG^CCA
MscI         1611  TGGCCA               TGG^CCA
NciI            4  CCCGG                CC^[GC]GG
NcoI         1930  CCATGG               C^CATGG
NheI         1510  GCTAGC               G^CTAGC
NspI         1821  ACATGT               [GA]CATG^[CT]
PflFI         589  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI         319  AGGACCC              [GA]G^G[AT]CC[CT]
PstI         1198  CTGCAG               CTGCA^G
StyI         1930  CCATGG               C^C[AT][AT]GG
Tth111I       589  GACTAAGTC            GAC[ACGT]^[ACGT][ACGT]GTC


###################################
# TWO CUT ENZYMES (   34 enzymes) #
###################################

AccII        1069  CGCG                 CG^CG
             1280  CGCG                
AlwNI         463  CAGTCTCTG            CAG[ACGT][ACGT][ACGT]^CTG
             1578  CAGTGCCTG           
Asp700I       172  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              856  GAAACCGTTC          
AvaII          31  GGTCC                G^G[AT]CC
              320  GGACC               
BbsI          758  GAAGAC               GAAGAC(2/6)
             1910  GTCTTC              
BcgI         2023  CGACATCATTGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
              445  GCAAAAAAGTCG        
BciVI        1753  GGATAC               GTATCC(6/5)
               69  GTATCC              
BfaI         1094  CTAG                 C^TAG
             1511  CTAG                
BpuAI         758  GAAGAC               GAAGAC(2/6)
             1910  GTCTTC              
BsaBI        1098  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1550  GATCTGCATC          
BsaI         1789  GAGACC               GGTCTC(1/5)
             1392  GGTCTC              
BsmBI        1022  CGTCTC               CGTCTC(1/5)
             1245  CGTCTC              
BsmFI         678  GGGAC                GGGAC(10/14)
              692  GGGAC               
BsrBI        1605  CCGCTC               CCGCTC(-3/-3)
             1708  CCGCTC              
BstUI        1069  CGCG                 CG^CG
             1280  CGCG                
BstYI        1097  GGATCC               [GA]^GATC[CT]
             1975  GGATCT              
CfoI         1070  GCGC                 GCG^C
             1327  GCGC                
HapII           5  CCGG                 C^CGG
               73  CCGG                
HgaI         1021  GCGTC                GACGC(5/10)
             1244  GCGTC               
HhaI         1070  GCGC                 GCG^C
             1327  GCGC                
HinP1I       1070  GCGC                 G^CGC
             1327  GCGC                
HindIII      1423  AAGCTT               A^AGCTT
             1565  AAGCTT              
HpaII           5  CCGG                 C^CGG
               73  CCGG                
MaeI         1094  CTAG                 C^TAG
             1511  CTAG                
MamI         1098  GATCCACATC           GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
             1550  GATCTGCATC          
MflI         1097  GGATCC               [GA]^GATC[CT]
             1975  GGATCT              
MspI            5  CCGG                 C^CGG
               73  CCGG                
MvnI         1069  CGCG                 CG^CG
             1280  CGCG                
MwoI          345  GCCCCAGTGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
              841  GCAGTGGGTGC         
SexAI         939  ACCTGGT              A^CC[AT]GGT
             1635  ACCTGGT             
TaqI           37  TCGA                 T^CGA
             1264  TCGA                
TthHB8I        37  TCGA                 T^CGA
             1264  TCGA                
XhoII        1097  GGATCC               [GA]^GATC[CT]
             1975  GGATCT              
XmnI          172  GAAATTTTTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
              856  GAAACCGTTC          


#########################################
# 3 OR MORE CUT ENZYMES (   67 enzymes) #
#########################################

Acc65I      AciI        AcsI        AfaI        AluI        
AlwI        ApoI        Asp718I     BanI        BbvI        
BmyI        BpmI        BsaJI       BsgI        BsiYI       
BslI        BsmAI       Bsp1286I    BspHI       BsrDI       
BsrI        BssKI       BstF5I      BstNI       Cac8I       
Cfr13I      DdeI        DpnII       DraII       EcoO109I    
EcoRII      Fnu4HI      FokI        HaeIII      HinfI       
HphI        ItaI        KpnI        MaeII       MaeIII      
MboI        MboII       MnlI        MseI        MslI        
MspA1I      MvaI        NdeII       NlaIII      NlaIV       
PleI        PvuII       RcaI        RsaI        Sau3AI      
Sau96I      ScrFI       SfaNI       SfcI        SmlI        
TfiI        Tru9I       TseI        Tsp45I      Tsp509I     
TspRI       XcmI