################################## # NO CUT ENZYMES ( 110 enzymes) # ################################## AatII AccIII AclI AflII AgeI AhdI Alw44I AocI Aor51HI ApaLI AscI AseI AsnI AspEI AspHI AviII AvrII BbeI BbrPI BbvCI BfrI BglII BlnI BlpI Bpu1102I BseAI BsiEI BsiHKAI BsiWI BsmI Bsp1407I BspEI BsrFI BsrGI BssHII BssSI BstBI BstEII BstPI Bsu36I CelII Cfr10I CpoI DraIII EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO65I EcoRV EcoT22I FseI FspI HaeII HincII HindII HpaI KasI KspI MfeI MluI MroI MunI NaeI NarI NdeI NgoMIV NotI NruI NsiI NspV PacI PaeR7I PflMI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SapI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SspI StuI SwaI Van91I XbaI XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 47 enzymes) # ################################### AccI 1693 GTATAC GT^[AC][GT]AC AcyI 1020 GGCGTC G[GA]^CG[CT]C AflIII 1821 ACATGT A^C[GA][CT]GT ApaI 1180 GGGCCC GGGCC^C AspI 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 195 CTCGGG C^[CT]CG[GA]G BalI 1611 TGGCCA TGG^CCA BamHI 1097 GGATCC G^GATCC BanII 1180 GGGCCC G[GA]GC[CT]^C BclI 751 TGATCA T^GATCA BcnI 4 CCCGG CC^[GC]GG BglI 345 GCCCCAGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BsaAI 1541 CACGTA [CT]AC^GT[GA] BsaHI 1020 GGCGTC G[GA]^CG[CT]C BsaWI 72 TCCGGT [AT]^CCGG[AT] BseRI 1805 CTCCTC GAGGAG(10/8) BsoBI 195 CTCGGG C^[CT]CG[GA]G BspDI 1263 ATCGAT AT^CGAT BspLU11I 1821 ACATGT A^CATGT BspMI 1660 GCAGGT ACCTGC(4/8) Bst1107I 1693 GTATAC GTA^TAC BstAPI 841 GCAGTGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 1629 CCAACAACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG BstZ17I 1693 GTATAC GTA^TAC ClaI 1263 ATCGAT AT^CGAT DraI 1937 TTTAAA TTT^AAA DrdI 632 GACGGCACTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 1930 CCATGG C^C[GA][CT]GG EaeI 1611 TGGCCA [CT]^GGCC[GA] EarI 1131 CTCTTC CTCTTC(1/4) EcoRI 1670 GAATTC G^AATTC EcoT14I 1930 CCATGG C^C[AT][AT]GG FauI 482 GCGGG CCCGC(4/6) FbaI 751 TGATCA T^GATCA Hin1I 1020 GGCGTC G[GA]^CG[CT]C Ksp632I 1131 CTCTTC CTCTTC(1/4) MluNI 1611 TGGCCA TGG^CCA MscI 1611 TGGCCA TGG^CCA NciI 4 CCCGG CC^[GC]GG NcoI 1930 CCATGG C^CATGG NheI 1510 GCTAGC G^CTAGC NspI 1821 ACATGT [GA]CATG^[CT] PflFI 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PpuMI 319 AGGACCC [GA]G^G[AT]CC[CT] PstI 1198 CTGCAG CTGCA^G StyI 1930 CCATGG C^C[AT][AT]GG Tth111I 589 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC ################################### # TWO CUT ENZYMES ( 34 enzymes) # ################################### AccII 1069 CGCG CG^CG 1280 CGCG AlwNI 463 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG 1578 CAGTGCCTG Asp700I 172 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 856 GAAACCGTTC AvaII 31 GGTCC G^G[AT]CC 320 GGACC BbsI 758 GAAGAC GAAGAC(2/6) 1910 GTCTTC BcgI 2023 CGACATCATTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) 445 GCAAAAAAGTCG BciVI 1753 GGATAC GTATCC(6/5) 69 GTATCC BfaI 1094 CTAG C^TAG 1511 CTAG BpuAI 758 GAAGAC GAAGAC(2/6) 1910 GTCTTC BsaBI 1098 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1550 GATCTGCATC BsaI 1789 GAGACC GGTCTC(1/5) 1392 GGTCTC BsmBI 1022 CGTCTC CGTCTC(1/5) 1245 CGTCTC BsmFI 678 GGGAC GGGAC(10/14) 692 GGGAC BsrBI 1605 CCGCTC CCGCTC(-3/-3) 1708 CCGCTC BstUI 1069 CGCG CG^CG 1280 CGCG BstYI 1097 GGATCC [GA]^GATC[CT] 1975 GGATCT CfoI 1070 GCGC GCG^C 1327 GCGC HapII 5 CCGG C^CGG 73 CCGG HgaI 1021 GCGTC GACGC(5/10) 1244 GCGTC HhaI 1070 GCGC GCG^C 1327 GCGC HinP1I 1070 GCGC G^CGC 1327 GCGC HindIII 1423 AAGCTT A^AGCTT 1565 AAGCTT HpaII 5 CCGG C^CGG 73 CCGG MaeI 1094 CTAG C^TAG 1511 CTAG MamI 1098 GATCCACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1550 GATCTGCATC MflI 1097 GGATCC [GA]^GATC[CT] 1975 GGATCT MspI 5 CCGG C^CGG 73 CCGG MvnI 1069 CGCG CG^CG 1280 CGCG MwoI 345 GCCCCAGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 841 GCAGTGGGTGC SexAI 939 ACCTGGT A^CC[AT]GGT 1635 ACCTGGT TaqI 37 TCGA T^CGA 1264 TCGA TthHB8I 37 TCGA T^CGA 1264 TCGA XhoII 1097 GGATCC [GA]^GATC[CT] 1975 GGATCT XmnI 172 GAAATTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 856 GAAACCGTTC ######################################### # 3 OR MORE CUT ENZYMES ( 67 enzymes) # ######################################### Acc65I AciI AcsI AfaI AluI AlwI ApoI Asp718I BanI BbvI BmyI BpmI BsaJI BsgI BsiYI BslI BsmAI Bsp1286I BspHI BsrDI BsrI BssKI BstF5I BstNI Cac8I Cfr13I DdeI DpnII DraII EcoO109I EcoRII Fnu4HI FokI HaeIII HinfI HphI ItaI KpnI MaeII MaeIII MboI MboII MnlI MseI MslI MspA1I MvaI NdeII NlaIII NlaIV PleI PvuII RcaI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XcmI