##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AocI Aor51HI AscI
AseI AsnI Asp718I AvrII BbeI
BbvCI BcgI BciVI BclI BfrI
BlnI BsaBI BsaHI BsaI BsaWI
BseAI BsiWI BsmBI BspDI BspEI
BspLU11I BssHII BssSI Bst1107I BstXI
BstZ17I Bsu36I ClaI CpoI DraI
DraIII EagI EclXI Eco47III Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
HgaI Hin1I HindIII HpaI KasI
KpnI KspI MamI MfeI MluI
MroI MunI NarI NdeI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PpuMI PshAI PshBI Psp1406I
RsrII SacII SalI SapI SbfI
ScaI SexAI SfoI SgrAI SnaBI
SpeI Sse8387I SspI StuI SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AccI 151 GTCTAC GT^[AC][GT]AC
AccII 1272 CGCG CG^CG
AcsI 334 GAATTC [GA]^AATT[CT]
AhdI 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 476 GTGCAC G^TGCAC
ApaI 1882 GGGCCC GGGCC^C
ApaLI 476 GTGCAC G^TGCAC
ApoI 334 GAATTC [GA]^AATT[CT]
AspEI 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 146 CCCGGG C^[CT]CG[GA]G
AviII 224 TGCGCA TGC^GCA
BanI 1263 GGTGCC G^G[CT][GA]CC
BbrPI 1814 CACGTG CAC^GTG
BbsI 328 GTCTTC GAAGAC(2/6)
BglII 1960 AGATCT A^GATCT
BlpI 1626 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 1626 GCTTAGC GC^T[ACGT]AGC
BpuAI 328 GTCTTC GAAGAC(2/6)
BsaAI 1814 CACGTG [CT]AC^GT[GA]
BsgI 825 CTGCAC GTGCAG(16/14)
BsiEI 809 CGATCG CG[GA][CT]^CG
BsmI 174 GAATGC GAATGC(1/-1)
BsoBI 146 CCCGGG C^[CT]CG[GA]G
BspHI 911 TCATGA T^CATGA
BsrBI 868 GAGCGG CCGCTC(-3/-3)
BsrDI 67 GCAATG GCAATG(2/0)
BsrFI 25 GCCGGC [GA]^CCGG[CT]
BstAPI 216 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 552 GGTGACC G^GT[ACGT]ACC
BstPI 552 GGTGACC G^GT[ACGT]ACC
BstUI 1272 CGCG CG^CG
CelII 1626 GCTTAGC GC^T[ACGT]AGC
Cfr10I 25 GCCGGC [GA]^CCGG[CT]
DsaI 323 CCATGG C^C[GA][CT]GG
Eam1105I 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 552 GGTGACC G^GT[ACGT]ACC
EcoRI 334 GAATTC G^AATTC
FseI 24 GGCCGGCC GGCCGG^CC
FspI 224 TGCGCA TGC^GCA
HaeII 1630 AGCGCC [GA]GCGC^[CT]
HincII 1714 GTTGAC GT[CT]^[GA]AC
HindII 1714 GTTGAC GT[CT]^[GA]AC
MaeII 1815 ACGT A^CGT
MvnI 1272 CGCG CG^CG
NaeI 25 GCCGGC GCC^GGC
NcoI 323 CCATGG C^CATGG
NgoMIV 25 GCCGGC G^CCGGC
NheI 1394 GCTAGC G^CTAGC
NspI 713 GCATGC [GA]CATG^[CT]
PflFI 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1814 CACGTG CAC^GTG
PmlI 1814 CACGTG CAC^GTG
PstI 1701 CTGCAG CTGCA^G
PvuI 809 CGATCG CGAT^CG
RcaI 911 TCATGA T^CATGA
SacI 1201 GAGCTC GAGCT^C
SfiI 19 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 146 CCCGGG CCC^GGG
SmlI 1285 CTCAAG C^T[CT][GA]AG
SphI 713 GCATGC GCATG^C
Tth111I 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 146 CCCGGG C^CCGGG
XmaI 146 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AflIII 1813 ACACGT A^C[GA][CT]GT
1815 ACGTGT
Asp700I 483 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1302 GAAGGTATTC
AvaII 275 GGTCC G^G[AT]CC
1444 GGTCC
BalI 18 TGGCCA TGG^CCA
1690 TGGCCA
BamHI 7 GGATCC G^GATCC
1058 GGATCC
BglI 20 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1367 GCCGCAGTGGC
BseRI 1669 CTCCTC GAGGAG(10/8)
388 GAGGAG
BsmAI 80 GAGAC GTCTC(1/5)
469 GAGAC
Bsp1407I 1810 TGTACA T^GTACA
1990 TGTACA
BsrGI 1810 TGTACA T^GTACA
1990 TGTACA
BstBI 331 TTCGAA TT^CGAA
1309 TTCGAA
DraII 1636 AGGCCCT [GA]G^G[ACGT]CC[CT]
1881 GGGGCCC
DrdI 742 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1333 GACCATGATGTC
EcoO109I 1636 AGGCCCT [GA]G^G[ACGT]CC[CT]
1881 GGGGCCC
EcoT14I 323 CCATGG C^C[AT][AT]GG
929 CCAAGG
MluNI 18 TGGCCA TGG^CCA
1690 TGGCCA
MscI 18 TGGCCA TGG^CCA
1690 TGGCCA
NspV 331 TTCGAA TT^CGAA
1309 TTCGAA
PflMI 278 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1139 CCATTGTATGG
PvuII 776 CAGCTG CAG^CTG
1410 CAGCTG
SfuI 331 TTCGAA TT^CGAA
1309 TTCGAA
SspBI 1810 TGTACA T^GTACA
1990 TGTACA
StyI 323 CCATGG C^C[AT][AT]GG
929 CCAAGG
TfiI 438 GATTC G^A[AT]TC
1177 GAATC
Tsp509I 335 AATT ^AATT
406 AATT
Van91I 278 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1139 CCATTGTATGG
XcmI 278 CCAACATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1547 CCACAGTGGAACTGG
XmnI 483 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1302 GAAGGTATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 74 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BsiHKAI BsiYI
BslI BsmFI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII EaeI
EarI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MflI MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI Tru9I TseI
Tsp45I TspRI TthHB8I XhoII