################################## # NO CUT ENZYMES ( 92 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AocI Aor51HI AscI AseI AsnI Asp718I AvrII BbeI BbvCI BcgI BciVI BclI BfrI BlnI BsaBI BsaHI BsaI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspLU11I BssHII BssSI Bst1107I BstXI BstZ17I Bsu36I ClaI CpoI DraI DraIII EagI EclXI Eco47III Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI HgaI Hin1I HindIII HpaI KasI KpnI KspI MamI MfeI MluI MroI MunI NarI NdeI NotI NruI NsiI PacI PaeR7I PinAI PmeI PpuMI PshAI PshBI Psp1406I RsrII SacII SalI SapI SbfI ScaI SexAI SfoI SgrAI SnaBI SpeI Sse8387I SspI StuI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 64 enzymes) # ################################### AccI 151 GTCTAC GT^[AC][GT]AC AccII 1272 CGCG CG^CG AcsI 334 GAATTC [GA]^AATT[CT] AhdI 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 476 GTGCAC G^TGCAC ApaI 1882 GGGCCC GGGCC^C ApaLI 476 GTGCAC G^TGCAC ApoI 334 GAATTC [GA]^AATT[CT] AspEI 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AspI 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC AvaI 146 CCCGGG C^[CT]CG[GA]G AviII 224 TGCGCA TGC^GCA BanI 1263 GGTGCC G^G[CT][GA]CC BbrPI 1814 CACGTG CAC^GTG BbsI 328 GTCTTC GAAGAC(2/6) BglII 1960 AGATCT A^GATCT BlpI 1626 GCTTAGC GC^T[ACGT]AGC Bpu1102I 1626 GCTTAGC GC^T[ACGT]AGC BpuAI 328 GTCTTC GAAGAC(2/6) BsaAI 1814 CACGTG [CT]AC^GT[GA] BsgI 825 CTGCAC GTGCAG(16/14) BsiEI 809 CGATCG CG[GA][CT]^CG BsmI 174 GAATGC GAATGC(1/-1) BsoBI 146 CCCGGG C^[CT]CG[GA]G BspHI 911 TCATGA T^CATGA BsrBI 868 GAGCGG CCGCTC(-3/-3) BsrDI 67 GCAATG GCAATG(2/0) BsrFI 25 GCCGGC [GA]^CCGG[CT] BstAPI 216 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 552 GGTGACC G^GT[ACGT]ACC BstPI 552 GGTGACC G^GT[ACGT]ACC BstUI 1272 CGCG CG^CG CelII 1626 GCTTAGC GC^T[ACGT]AGC Cfr10I 25 GCCGGC [GA]^CCGG[CT] DsaI 323 CCATGG C^C[GA][CT]GG Eam1105I 46 GACTCCAGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EcoO65I 552 GGTGACC G^GT[ACGT]ACC EcoRI 334 GAATTC G^AATTC FseI 24 GGCCGGCC GGCCGG^CC FspI 224 TGCGCA TGC^GCA HaeII 1630 AGCGCC [GA]GCGC^[CT] HincII 1714 GTTGAC GT[CT]^[GA]AC HindII 1714 GTTGAC GT[CT]^[GA]AC MaeII 1815 ACGT A^CGT MvnI 1272 CGCG CG^CG NaeI 25 GCCGGC GCC^GGC NcoI 323 CCATGG C^CATGG NgoMIV 25 GCCGGC G^CCGGC NheI 1394 GCTAGC G^CTAGC NspI 713 GCATGC [GA]CATG^[CT] PflFI 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC PmaCI 1814 CACGTG CAC^GTG PmlI 1814 CACGTG CAC^GTG PstI 1701 CTGCAG CTGCA^G PvuI 809 CGATCG CGAT^CG RcaI 911 TCATGA T^CATGA SacI 1201 GAGCTC GAGCT^C SfiI 19 GGCCAGGCCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC SmaI 146 CCCGGG CCC^GGG SmlI 1285 CTCAAG C^T[CT][GA]AG SphI 713 GCATGC GCATG^C Tth111I 1821 GACTTTGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 146 CCCGGG C^CCGGG XmaI 146 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AflIII 1813 ACACGT A^C[GA][CT]GT 1815 ACGTGT Asp700I 483 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1302 GAAGGTATTC AvaII 275 GGTCC G^G[AT]CC 1444 GGTCC BalI 18 TGGCCA TGG^CCA 1690 TGGCCA BamHI 7 GGATCC G^GATCC 1058 GGATCC BglI 20 GCCAGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1367 GCCGCAGTGGC BseRI 1669 CTCCTC GAGGAG(10/8) 388 GAGGAG BsmAI 80 GAGAC GTCTC(1/5) 469 GAGAC Bsp1407I 1810 TGTACA T^GTACA 1990 TGTACA BsrGI 1810 TGTACA T^GTACA 1990 TGTACA BstBI 331 TTCGAA TT^CGAA 1309 TTCGAA DraII 1636 AGGCCCT [GA]G^G[ACGT]CC[CT] 1881 GGGGCCC DrdI 742 GACAAGATTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1333 GACCATGATGTC EcoO109I 1636 AGGCCCT [GA]G^G[ACGT]CC[CT] 1881 GGGGCCC EcoT14I 323 CCATGG C^C[AT][AT]GG 929 CCAAGG MluNI 18 TGGCCA TGG^CCA 1690 TGGCCA MscI 18 TGGCCA TGG^CCA 1690 TGGCCA NspV 331 TTCGAA TT^CGAA 1309 TTCGAA PflMI 278 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1139 CCATTGTATGG PvuII 776 CAGCTG CAG^CTG 1410 CAGCTG SfuI 331 TTCGAA TT^CGAA 1309 TTCGAA SspBI 1810 TGTACA T^GTACA 1990 TGTACA StyI 323 CCATGG C^C[AT][AT]GG 929 CCAAGG TfiI 438 GATTC G^A[AT]TC 1177 GAATC Tsp509I 335 AATT ^AATT 406 AATT Van91I 278 CCAACATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1139 CCATTGTATGG XcmI 278 CCAACATCTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1547 CCACAGTGGAACTGG XmnI 483 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 1302 GAAGGTATTC ######################################### # 3 OR MORE CUT ENZYMES ( 74 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI BanII BbvI BcnI BfaI BmyI BpmI BsaJI BsiHKAI BsiYI BslI BsmFI Bsp1286I BspMI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII EaeI EarI EcoRII FauI Fnu4HI FokI HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV PleI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XhoII