##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AhdI AlwNI AocI Aor51HI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AviII AvrII BbrPI BbvCI
BclI BglII BlnI BsaAI BsaI
BseAI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI Bst1107I
BstBI BstEII BstPI BstZ17I Bsu36I
ClaI DraI DraIII DrdI Eam1105I
Eco47III Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI FspI KspI
MfeI MroI MunI NdeI NheI
NotI NruI NsiI NspV PacI
PflFI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI SacI SacII
SalI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I XbaI XmnI
###################################
# ONE CUT ENZYMES ( 66 enzymes) #
###################################
Acc65I 1552 GGTACC G^GTACC
AcyI 499 GGCGCC G[GA]^CG[CT]C
AflII 435 CTTAAG C^TTAAG
AflIII 617 ACGCGT A^C[GA][CT]GT
AgeI 1804 ACCGGT A^CCGGT
Alw44I 153 GTGCAC G^TGCAC
ApaI 262 GGGCCC GGGCC^C
ApaLI 153 GTGCAC G^TGCAC
Asp718I 1552 GGTACC G^GTACC
AspHI 153 GTGCAC G[AT]GC[AT]^C
BamHI 55 GGATCC G^GATCC
BbeI 499 GGCGCC GGCGC^C
BcgI 217 CGACCGTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 1417 GGATAC GTATCC(6/5)
BfaI 1946 CTAG C^TAG
BfrI 435 CTTAAG C^TTAAG
BlpI 685 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 685 GCTCAGC GC^T[ACGT]AGC
BsaBI 1907 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 499 GGCGCC G[GA]^CG[CT]C
BsaWI 1804 ACCGGT [AT]^CCGG[AT]
BsgI 696 CTGCAC GTGCAG(16/14)
BsiHKAI 153 GTGCAC G[AT]GC[AT]^C
BsmAI 1321 GAGAC GTCTC(1/5)
BspHI 898 TCATGA T^CATGA
BsrBI 405 CCGCTC CCGCTC(-3/-3)
BssSI 468 CTCGTG CACGAG(-5/-1)
BstAPI 665 GCACAAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 55 GGATCC [GA]^GATC[CT]
CelII 685 GCTCAGC GC^T[ACGT]AGC
CpoI 202 CGGACCG CG^G[AT]CCG
EagI 402 CGGCCG C^GGCCG
EclXI 402 CGGCCG C^GGCCG
Eco52I 402 CGGCCG C^GGCCG
EcoNI 791 CCTTCCCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 775 GACGC GACGC(5/10)
Hin1I 499 GGCGCC G[GA]^CG[CT]C
HincII 1223 GTTAAC GT[CT]^[GA]AC
HindII 1223 GTTAAC GT[CT]^[GA]AC
HindIII 1471 AAGCTT A^AGCTT
HpaI 1223 GTTAAC GTT^AAC
HphI 409 TCACC GGTGA(8/7)
KasI 499 GGCGCC G^GCGCC
KpnI 1552 GGTACC GGTAC^C
MaeI 1946 CTAG C^TAG
MamI 1907 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 55 GGATCC [GA]^GATC[CT]
MluI 617 ACGCGT A^CGCGT
NarI 499 GGCGCC GG^CGCC
NspI 1594 GCATGT [GA]CATG^[CT]
PaeR7I 1294 CTCGAG C^TCGAG
PflMI 86 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1804 ACCGGT A^CCGGT
PpuMI 1028 AGGACCT [GA]G^G[AT]CC[CT]
PstI 138 CTGCAG CTGCA^G
RcaI 898 TCATGA T^CATGA
RsrII 202 CGGACCG CG^G[AT]CCG
SapI 723 GAAGAGC GCTCTTC(1/4)
SexAI 1555 ACCTGGT A^CC[AT]GGT
SfoI 499 GGCGCC GGC^GCC
SspI 1708 AATATT AAT^ATT
StuI 1392 AGGCCT AGG^CCT
TfiI 442 GAATC G^A[AT]TC
Van91I 86 CCAGCGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 1294 CTCGAG C^TCGAG
XhoII 55 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AfaI 1214 GTAC GT^AC
1553 GTAC
AlwI 56 GATCC GGATC(4/5)
55 GGATC
BbsI 458 GAAGAC GAAGAC(2/6)
579 GAAGAC
BglI 502 GCCGTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
997 GCCAGAAAGGC
BpmI 165 CTGGAG CTGGAG(16/14)
171 CTGGAG
BpuAI 458 GAAGAC GAAGAC(2/6)
579 GAAGAC
BsmI 1103 GCATTC GAATGC(1/-1)
1857 GCATTC
BspMI 190 ACCTGC ACCTGC(4/8)
1549 GCAGGT
BstF5I 527 GGATG GGATG(2/0)
1545 GGATG
BstXI 739 CCATGCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1604 CCAGTAACCTGG
DpnII 56 GATC ^GATC
548 GATC
DraII 262 GGGCCCT [GA]G^G[ACGT]CC[CT]
1028 AGGACCT
EcoO109I 262 GGGCCCT [GA]G^G[ACGT]CC[CT]
1028 AGGACCT
FokI 527 GGATG GGATG(9/13)
1545 GGATG
MaeII 29 ACGT A^CGT
925 ACGT
MboI 56 GATC ^GATC
548 GATC
NaeI 690 GCCGGC GCC^GGC
1021 GCCGGC
NcoI 76 CCATGG C^CATGG
574 CCATGG
NdeII 56 GATC ^GATC
548 GATC
NgoMIV 690 GCCGGC G^CCGGC
1021 GCCGGC
PvuII 285 CAGCTG CAG^CTG
300 CAGCTG
RsaI 1214 GTAC GT^AC
1553 GTAC
Sau3AI 56 GATC ^GATC
548 GATC
SfcI 16 CTGTAG C^T[GA][CT]AG
138 CTGCAG
SmaI 523 CCCGGG CCC^GGG
811 CCCGGG
Tsp45I 927 GTGAC ^GT[GC]AC
1258 GTGAC
XcmI 760 CCACCGCTTCGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1872 CCAAGGCTGCCCTGG
XmaCI 523 CCCGGG C^CCGGG
811 CCCGGG
XmaI 523 CCCGGG C^CCGGG
811 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AluI AvaI AvaII
BalI BanI BanII BbvI BcnI
BmyI BsaJI BseRI BsiEI BsiYI
BslI BsmFI BsoBI Bsp1286I BsrFI
BsrI BssHII BssKI BstNI BstUI
Cac8I CfoI Cfr10I Cfr13I DdeI
DsaI EaeI EarI EcoRII EcoT14I
FauI Fnu4HI HaeII HaeIII HapII
HhaI HinP1I HinfI HpaII ItaI
Ksp632I MaeIII MboII MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV PleI Sau96I ScrFI SfaNI
SmlI StyI TaqI Tru9I TseI
Tsp509I TspRI TthHB8I