################################## # NO CUT ENZYMES ( 77 enzymes) # ################################## AflII AgeI AhdI Alw44I AocI ApaLI AscI AseI AsnI AspEI AspI AviII AvrII BciVI BfrI BglII BlnI BsaBI BsiEI BsiWI BsmBI BsmI BspDI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I Bsu36I ClaI CpoI DrdI EagI Eam1105I EclXI Eco52I Eco81I EcoO65I EcoRV EcoT22I FseI FspI HpaI MamI MfeI MluI MunI NdeI NheI NotI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SalI SapI SbfI SfiI SfuI SgrAI SnaBI SpeI Sse8387I SspI SwaI Tth111I XbaI XhoI ################################### # ONE CUT ENZYMES ( 44 enzymes) # ################################### AatII 905 GACGTC GACGT^C AclI 2700 AACGTT AA^CGTT Aor51HI 773 AGCGCT AGC^GCT BamHI 567 GGATCC G^GATCC BbeI 1655 GGCGCC GGCGC^C BbrPI 1411 CACGTG CAC^GTG BbsI 1210 GAAGAC GAAGAC(2/6) BcgI 2188 GCAGAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BpuAI 1210 GAAGAC GAAGAC(2/6) BsaAI 1411 CACGTG [CT]AC^GT[GA] Bsp1407I 319 TGTACA T^GTACA BspHI 1383 TCATGA T^CATGA BspLU11I 30 ACATGT A^CATGT BsrDI 1726 CATTGC GCAATG(2/0) BsrGI 319 TGTACA T^GTACA BstAPI 172 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC DraI 110 TTTAAA TTT^AAA DraIII 1034 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG EarI 3104 GAAGAG CTCTTC(1/4) Eco47III 773 AGCGCT AGC^GCT HincII 2269 GTTGAC GT[CT]^[GA]AC HindII 2269 GTTGAC GT[CT]^[GA]AC KasI 1655 GGCGCC G^GCGCC Ksp632I 3104 GAAGAG CTCTTC(1/4) KspI 238 CCGCGG CCGC^GG MseI 111 TTAA T^TAA MslI 3058 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG NarI 1655 GGCGCC GG^CGCC NruI 2608 TCGCGA TCG^CGA PmaCI 1411 CACGTG CAC^GTG PmlI 1411 CACGTG CAC^GTG Psp1406I 2700 AACGTT AA^CGTT RcaI 1383 TCATGA T^CATGA SacII 238 CCGCGG CCGC^GG ScaI 2097 AGTACT AGT^ACT SexAI 1839 ACCTGGT A^CC[AT]GGT SfoI 1655 GGCGCC GGC^GCC SmlI 965 CTCAAG C^T[CT][GA]AG SphI 2405 GCATGC GCATG^C SspBI 319 TGTACA T^GTACA TaqI 1551 TCGA T^CGA TfiI 2112 GAATC G^A[AT]TC Tru9I 111 TTAA T^TAA TthHB8I 1551 TCGA T^CGA ################################### # TWO CUT ENZYMES ( 29 enzymes) # ################################### Acc65I 1267 GGTACC G^GTACC 2488 GGTACC AccIII 99 TCCGGA T^CCGGA 651 TCCGGA ApaI 1123 GGGCCC GGGCC^C 1455 GGGCCC Asp700I 2095 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2727 GAAGCCCTTC Asp718I 1267 GGTACC G^GTACC 2488 GGTACC BbvCI 1991 GCTGAGG CCTCAGC(-5/-2) 2632 GCTGAGG BclI 1386 TGATCA T^GATCA 1545 TGATCA BlpI 1643 GCTCAGC GC^T[ACGT]AGC 2381 GCTGAGC Bpu1102I 1643 GCTCAGC GC^T[ACGT]AGC 2381 GCTGAGC BsaWI 99 TCCGGA [AT]^CCGG[AT] 651 TCCGGA BseAI 99 TCCGGA T^CCGGA 651 TCCGGA BspEI 99 TCCGGA T^CCGGA 651 TCCGGA BsrFI 374 GCCGGC [GA]^CCGG[CT] 1416 GCCGGC BstXI 1563 CCACTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3000 CCAGACCACTGG CelII 1643 GCTCAGC GC^T[ACGT]AGC 2381 GCTGAGC Cfr10I 374 GCCGGC [GA]^CCGG[CT] 1416 GCCGGC EcoRI 2291 GAATTC G^AATTC 2366 GAATTC FbaI 1386 TGATCA T^GATCA 1545 TGATCA HaeII 773 AGCGCT [GA]GCGC^[CT] 1655 GGCGCC HindIII 16 AAGCTT A^AGCTT 1817 AAGCTT KpnI 1267 GGTACC GGTAC^C 2488 GGTACC MroI 99 TCCGGA T^CCGGA 651 TCCGGA NaeI 374 GCCGGC GCC^GGC 1416 GCCGGC NgoMIV 374 GCCGGC G^CCGGC 1416 GCCGGC SacI 77 GAGCTC GAGCT^C 1109 GAGCTC SmaI 1452 CCCGGG CCC^GGG 2310 CCCGGG XmaCI 1452 CCCGGG C^CCGGG 2310 CCCGGG XmaI 1452 CCCGGG C^CCGGG 2310 CCCGGG XmnI 2095 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2727 GAAGCCCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 108 enzymes) # ######################################### AccI AccII AciI AcsI AcyI AfaI AflIII AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NciI NcoI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TseI Tsp45I Tsp509I TspRI Van91I XcmI XhoII