##################################
# NO CUT ENZYMES ( 77 enzymes) #
##################################
AflII AgeI AhdI Alw44I AocI
ApaLI AscI AseI AsnI AspEI
AspI AviII AvrII BciVI BfrI
BglII BlnI BsaBI BsiEI BsiWI
BsmBI BsmI BspDI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I ClaI CpoI DrdI EagI
Eam1105I EclXI Eco52I Eco81I EcoO65I
EcoRV EcoT22I FseI FspI HpaI
MamI MfeI MluI MunI NdeI
NheI NotI NsiI NspV PacI
PaeR7I PflFI PinAI PmeI PshAI
PshBI PvuI RsrII SalI SapI
SbfI SfiI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tth111I
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AatII 905 GACGTC GACGT^C
AclI 2700 AACGTT AA^CGTT
Aor51HI 773 AGCGCT AGC^GCT
BamHI 567 GGATCC G^GATCC
BbeI 1655 GGCGCC GGCGC^C
BbrPI 1411 CACGTG CAC^GTG
BbsI 1210 GAAGAC GAAGAC(2/6)
BcgI 2188 GCAGAGGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1210 GAAGAC GAAGAC(2/6)
BsaAI 1411 CACGTG [CT]AC^GT[GA]
Bsp1407I 319 TGTACA T^GTACA
BspHI 1383 TCATGA T^CATGA
BspLU11I 30 ACATGT A^CATGT
BsrDI 1726 CATTGC GCAATG(2/0)
BsrGI 319 TGTACA T^GTACA
BstAPI 172 GCAGGACCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 110 TTTAAA TTT^AAA
DraIII 1034 CACACGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 3104 GAAGAG CTCTTC(1/4)
Eco47III 773 AGCGCT AGC^GCT
HincII 2269 GTTGAC GT[CT]^[GA]AC
HindII 2269 GTTGAC GT[CT]^[GA]AC
KasI 1655 GGCGCC G^GCGCC
Ksp632I 3104 GAAGAG CTCTTC(1/4)
KspI 238 CCGCGG CCGC^GG
MseI 111 TTAA T^TAA
MslI 3058 CATCCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 1655 GGCGCC GG^CGCC
NruI 2608 TCGCGA TCG^CGA
PmaCI 1411 CACGTG CAC^GTG
PmlI 1411 CACGTG CAC^GTG
Psp1406I 2700 AACGTT AA^CGTT
RcaI 1383 TCATGA T^CATGA
SacII 238 CCGCGG CCGC^GG
ScaI 2097 AGTACT AGT^ACT
SexAI 1839 ACCTGGT A^CC[AT]GGT
SfoI 1655 GGCGCC GGC^GCC
SmlI 965 CTCAAG C^T[CT][GA]AG
SphI 2405 GCATGC GCATG^C
SspBI 319 TGTACA T^GTACA
TaqI 1551 TCGA T^CGA
TfiI 2112 GAATC G^A[AT]TC
Tru9I 111 TTAA T^TAA
TthHB8I 1551 TCGA T^CGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
Acc65I 1267 GGTACC G^GTACC
2488 GGTACC
AccIII 99 TCCGGA T^CCGGA
651 TCCGGA
ApaI 1123 GGGCCC GGGCC^C
1455 GGGCCC
Asp700I 2095 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2727 GAAGCCCTTC
Asp718I 1267 GGTACC G^GTACC
2488 GGTACC
BbvCI 1991 GCTGAGG CCTCAGC(-5/-2)
2632 GCTGAGG
BclI 1386 TGATCA T^GATCA
1545 TGATCA
BlpI 1643 GCTCAGC GC^T[ACGT]AGC
2381 GCTGAGC
Bpu1102I 1643 GCTCAGC GC^T[ACGT]AGC
2381 GCTGAGC
BsaWI 99 TCCGGA [AT]^CCGG[AT]
651 TCCGGA
BseAI 99 TCCGGA T^CCGGA
651 TCCGGA
BspEI 99 TCCGGA T^CCGGA
651 TCCGGA
BsrFI 374 GCCGGC [GA]^CCGG[CT]
1416 GCCGGC
BstXI 1563 CCACTCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3000 CCAGACCACTGG
CelII 1643 GCTCAGC GC^T[ACGT]AGC
2381 GCTGAGC
Cfr10I 374 GCCGGC [GA]^CCGG[CT]
1416 GCCGGC
EcoRI 2291 GAATTC G^AATTC
2366 GAATTC
FbaI 1386 TGATCA T^GATCA
1545 TGATCA
HaeII 773 AGCGCT [GA]GCGC^[CT]
1655 GGCGCC
HindIII 16 AAGCTT A^AGCTT
1817 AAGCTT
KpnI 1267 GGTACC GGTAC^C
2488 GGTACC
MroI 99 TCCGGA T^CCGGA
651 TCCGGA
NaeI 374 GCCGGC GCC^GGC
1416 GCCGGC
NgoMIV 374 GCCGGC G^CCGGC
1416 GCCGGC
SacI 77 GAGCTC GAGCT^C
1109 GAGCTC
SmaI 1452 CCCGGG CCC^GGG
2310 CCCGGG
XmaCI 1452 CCCGGG C^CCGGG
2310 CCCGGG
XmaI 1452 CCCGGG C^CCGGG
2310 CCCGGG
XmnI 2095 GAAGTACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2727 GAAGCCCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccI AccII AciI AcsI AcyI
AfaI AflIII AluI AlwI AlwNI
ApoI AspHI AvaI AvaII BalI
BanI BanII BbvI BcnI BfaI
BglI BmyI BpmI BsaHI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrBI BsrI BssKI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MspA1I MspI MvaI
MvnI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StuI
StyI TseI Tsp45I Tsp509I TspRI
Van91I XcmI XhoII