##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
Acc65I AclI AcsI AflII AflIII
AhdI AocI ApoI AscI AseI
AsnI Asp718I AspEI AspI BbeI
BciVI BclI BfrI BglII BsaBI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspLU11I BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I Eco81I EcoRI EcoRV EcoT22I
FbaI FseI HpaI KasI KpnI
KspI MamI MfeI MluI MunI
NarI NotI NruI NsiI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RsrII SacII
SalI ScaI SfiI SfoI SfuI
SgrAI SmaI SnaBI SspBI SspI
SwaI Tth111I XhoI XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 1885 GACGTC GACGT^C
AccI 2021 GTCTAC GT^[AC][GT]AC
AccIII 1095 TCCGGA T^CCGGA
AcyI 1885 GACGTC G[GA]^CG[CT]C
AgeI 1615 ACCGGT A^CCGGT
Aor51HI 1422 AGCGCT AGC^GCT
Asp700I 1687 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2073 TGCGCA TGC^GCA
AvrII 2732 CCTAGG C^CTAGG
BamHI 2543 GGATCC G^GATCC
BbrPI 1366 CACGTG CAC^GTG
BbvCI 1870 CCTCAGC CCTCAGC(-5/-2)
BglI 795 GCCGTCAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 2732 CCTAGG C^CTAGG
BsaHI 1885 GACGTC G[GA]^CG[CT]C
BseAI 1095 TCCGGA T^CCGGA
BspEI 1095 TCCGGA T^CCGGA
BspHI 2236 TCATGA T^CATGA
BssSI 340 CACGAG CACGAG(-5/-1)
BstXI 1227 CCAGCAAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Eco47III 1422 AGCGCT AGC^GCT
FspI 2073 TGCGCA TGC^GCA
Hin1I 1885 GACGTC G[GA]^CG[CT]C
HincII 2557 GTCAAC GT[CT]^[GA]AC
HindII 2557 GTCAAC GT[CT]^[GA]AC
HindIII 1703 AAGCTT A^AGCTT
MroI 1095 TCCGGA T^CCGGA
MseI 1242 TTAA T^TAA
NaeI 471 GCCGGC GCC^GGC
NcoI 1122 CCATGG C^CATGG
NdeI 1348 CATATG CA^TATG
NgoMIV 471 GCCGGC G^CCGGC
NheI 352 GCTAGC G^CTAGC
PinAI 1615 ACCGGT A^CCGGT
PmaCI 1366 CACGTG CAC^GTG
PmlI 1366 CACGTG CAC^GTG
PpuMI 20 GGGACCC [GA]G^G[AT]CC[CT]
RcaI 2236 TCATGA T^CATGA
SacI 2358 GAGCTC GAGCT^C
SbfI 2209 CCTGCAGG CCTGCA^GG
SexAI 390 ACCTGGT A^CC[AT]GGT
SpeI 962 ACTAGT A^CTAGT
SphI 1540 GCATGC GCATG^C
Sse8387I 2209 CCTGCAGG CCTGCA^GG
Tru9I 1242 TTAA T^TAA
Tsp509I 1103 AATT ^AATT
XbaI 2464 TCTAGA T^CTAGA
XmnI 1687 GAAGGACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
Alw44I 551 GTGCAC G^TGCAC
1305 GTGCAC
ApaI 925 GGGCCC GGGCC^C
1174 GGGCCC
ApaLI 551 GTGCAC G^TGCAC
1305 GTGCAC
BcgI 1823 GCATCCTCCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2398 GCAAGACCTTCG
BcnI 310 CCGGG CC^[GC]GG
1930 CCGGG
BlpI 1339 GCTGAGC GC^T[ACGT]AGC
2249 GCTCAGC
Bpu1102I 1339 GCTGAGC GC^T[ACGT]AGC
2249 GCTCAGC
BsaAI 1211 TACGTG [CT]AC^GT[GA]
1366 CACGTG
BsaI 575 GAGACC GGTCTC(1/5)
1618 GGTCTC
BsaWI 1095 TCCGGA [AT]^CCGG[AT]
1615 ACCGGT
BsgI 1265 CTGCAC GTGCAG(16/14)
419 GTGCAG
BsrBI 1354 CCGCTC CCGCTC(-3/-3)
1535 GAGCGG
BstEII 1465 GGTCACC G^GT[ACGT]ACC
2203 GGTGACC
BstPI 1465 GGTCACC G^GT[ACGT]ACC
2203 GGTGACC
CelII 1339 GCTGAGC GC^T[ACGT]AGC
2249 GCTCAGC
EcoNI 391 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1012 CCTGGGGCAGG
EcoO65I 1465 GGTCACC G^GT[ACGT]ACC
2203 GGTGACC
HgaI 347 GACGC GACGC(5/10)
1312 GACGC
MslI 554 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1541 CATGCAGATG
NciI 310 CCGGG CC^[GC]GG
1930 CCGGG
NspI 1540 GCATGC [GA]CATG^[CT]
2534 ACATGC
SapI 1727 GAAGAGC GCTCTTC(1/4)
1519 GCTCTTC
SmlI 491 CTTGAG C^T[CT][GA]AG
2243 CTTGAG
StuI 50 AGGCCT AGG^CCT
669 AGGCCT
XcmI 1029 CCAAGGACTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2305 CCAGCTCCTGCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 100 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BfaI
BmyI BpmI BpuAI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BspMI BsrDI BsrFI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DrdI
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I TspRI TthHB8I Van91I XhoII