##################################
# NO CUT ENZYMES ( 81 enzymes) #
##################################
Acc65I AccI AccIII AclI AgeI
AhdI AscI Asp718I AspEI AviII
AvrII BalI BbeI BlnI BlpI
Bpu1102I BseAI BsiEI BsiWI Bsp1407I
BspEI BsrFI BsrGI BssHII Bst1107I
BstBI BstZ17I CelII Cfr10I CpoI
DrdI EaeI EagI Eam1105I EclXI
Eco52I EcoRI EcoRV FseI FspI
KasI KpnI KspI MfeI MluI
MluNI MroI MscI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspV PacI PaeR7I PinAI
PmeI PpuMI PshAI Psp1406I PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfoI SfuI SgrAI SnaBI
SpeI SspBI SspI StuI XcmI
XhoI
###################################
# ONE CUT ENZYMES ( 62 enzymes) #
###################################
AccII 1361 CGCG CG^CG
AflII 534 CTTAAG C^TTAAG
Alw44I 1007 GTGCAC G^TGCAC
AlwNI 2845 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 3045 CCTCAGG CC^T[ACGT]AGG
Aor51HI 1415 AGCGCT AGC^GCT
ApaI 1582 GGGCCC GGGCC^C
ApaLI 1007 GTGCAC G^TGCAC
AseI 2641 ATTAAT AT^TAAT
AsnI 2641 ATTAAT AT^TAAT
AspI 2176 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BamHI 962 GGATCC G^GATCC
BanI 3098 GGTGCC G^G[CT][GA]CC
BbrPI 2441 CACGTG CAC^GTG
BcgI 1841 GCAAAGCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2037 TGATCA T^GATCA
BfrI 534 CTTAAG C^TTAAG
BglII 788 AGATCT A^GATCT
BsaAI 2441 CACGTG [CT]AC^GT[GA]
BsaBI 2200 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2269 ACCGGA [AT]^CCGG[AT]
BsmI 1404 GAATGC GAATGC(1/-1)
BspDI 2784 ATCGAT AT^CGAT
BspHI 1805 TCATGA T^CATGA
BsrBI 2282 GAGCGG CCGCTC(-3/-3)
BssSI 1862 CTCGTG CACGAG(-5/-1)
BstAPI 2462 GCACTTACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 1798 GGTCACC G^GT[ACGT]ACC
BstPI 1798 GGTCACC G^GT[ACGT]ACC
BstUI 1361 CGCG CG^CG
BstXI 3087 CCAAGGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 3045 CCTCAGG CC^T[ACGT]AGG
ClaI 2784 ATCGAT AT^CGAT
DraI 1686 TTTAAA TTT^AAA
Eco47III 1415 AGCGCT AGC^GCT
Eco81I 3045 CCTCAGG CC^T[ACGT]AGG
EcoNI 1061 CCTCGAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1798 GGTCACC G^GT[ACGT]ACC
EcoT22I 446 ATGCAT ATGCA^T
FauI 2314 GCGGG CCCGC(4/6)
FbaI 2037 TGATCA T^GATCA
HincII 3174 GTTAAC GT[CT]^[GA]AC
HindII 3174 GTTAAC GT[CT]^[GA]AC
HindIII 2116 AAGCTT A^AGCTT
HpaI 3174 GTTAAC GTT^AAC
MamI 2200 GATGCCAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MvnI 1361 CGCG CG^CG
NsiI 446 ATGCAT ATGCA^T
PflFI 2176 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 2441 CACGTG CAC^GTG
PmlI 2441 CACGTG CAC^GTG
PshBI 2641 ATTAAT AT^TAAT
RcaI 1805 TCATGA T^CATGA
SbfI 1397 CCTGCAGG CCTGCA^GG
SmaI 1954 CCCGGG CCC^GGG
SphI 1424 GCATGC GCATG^C
Sse8387I 1397 CCTGCAGG CCTGCA^GG
SwaI 1685 ATTTAAAT ATTT^AAAT
Tth111I 2176 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 576 TCTAGA T^CTAGA
XmaCI 1954 CCCGGG C^CCGGG
XmaI 1954 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AatII 1554 GACGTC GACGT^C
2966 GACGTC
AflIII 1930 ACATGT A^C[GA][CT]GT
2600 ACATGT
Asp700I 925 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2484 GAAAGTCTTC
BbsI 1081 GAAGAC GAAGAC(2/6)
2488 GTCTTC
BbvCI 1836 CCTCAGC CCTCAGC(-5/-2)
709 GCTGAGG
BpuAI 1081 GAAGAC GAAGAC(2/6)
2488 GTCTTC
BsaI 677 GAGACC GGTCTC(1/5)
3200 GAGACC
BsmBI 1552 GAGACG CGTCTC(1/5)
1854 GAGACG
BspLU11I 1930 ACATGT A^CATGT
2600 ACATGT
BspMI 1122 ACCTGC ACCTGC(4/8)
97 GCAGGT
BstYI 788 AGATCT [GA]^GATC[CT]
962 GGATCC
DraII 1581 AGGGCCC [GA]G^G[ACGT]CC[CT]
1621 AGGCCCC
EcoO109I 1581 AGGGCCC [GA]G^G[ACGT]CC[CT]
1621 AGGCCCC
HaeII 474 AGCGCC [GA]GCGC^[CT]
1415 AGCGCT
MflI 788 AGATCT [GA]^GATC[CT]
962 GGATCC
NcoI 3168 CCATGG C^CATGG
3195 CCATGG
SacI 1650 GAGCTC GAGCT^C
3131 GAGCTC
SapI 978 GAAGAGC GCTCTTC(1/4)
3133 GCTCTTC
SmlI 496 CTTGAG C^T[CT][GA]AG
534 CTTAAG
XhoII 788 AGATCT [GA]^GATC[CT]
962 GGATCC
XmnI 925 GAAAACCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2484 GAAAGTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AspHI AvaI AvaII
BanII BbvI BciVI BcnI BfaI
BglI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraIII
DsaI EarI EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PflMI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I