##################################
# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccI AclI AflII AflIII
AgeI AhdI AscI AseI AsnI
AspEI AspI AviII AvrII BbrPI
BbvCI BcgI BciVI BclI BfrI
BglII BlnI BpmI BsaAI BsaBI
BsiWI BsmBI Bsp1407I BspDI BspHI
BspLU11I BsrDI BsrFI BsrGI BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
Cfr10I ClaI CpoI Eam1105I EcoO65I
EcoRV EcoT22I FbaI FseI FspI
MamI MfeI MluI MunI NaeI
NgoMIV NotI NruI NsiI PacI
PflFI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SalI SbfI ScaI
SexAI SgrAI SnaBI SpeI Sse8387I
SspBI SspI SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
Acc65I 1472 GGTACC G^GTACC
AccIII 128 TCCGGA T^CCGGA
Alw44I 1656 GTGCAC G^TGCAC
AlwNI 2905 CAGAAACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2606 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1296 AGCGCT AGC^GCT
ApaI 2969 GGGCCC GGGCC^C
ApaLI 1656 GTGCAC G^TGCAC
Asp700I 2914 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1472 GGTACC G^GTACC
BalI 3087 TGGCCA TGG^CCA
BamHI 2602 GGATCC G^GATCC
BbeI 2264 GGCGCC GGCGC^C
BglI 1687 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BseAI 128 TCCGGA T^CCGGA
BsiEI 35 CGGCCG CG[GA][CT]^CG
BsmI 2074 GCATTC GAATGC(1/-1)
BspEI 128 TCCGGA T^CCGGA
BssHII 88 GCGCGC G^CGCGC
Bsu36I 2606 CCTAAGG CC^T[ACGT]AGG
DraI 1750 TTTAAA TTT^AAA
DraIII 1698 CACAGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 849 GACCAGCATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 35 CGGCCG C^GGCCG
EclXI 35 CGGCCG C^GGCCG
Eco47III 1296 AGCGCT AGC^GCT
Eco52I 35 CGGCCG C^GGCCG
Eco81I 2606 CCTAAGG CC^T[ACGT]AGG
EcoRI 1342 GAATTC G^AATTC
HincII 220 GTTAAC GT[CT]^[GA]AC
HindII 220 GTTAAC GT[CT]^[GA]AC
HpaI 220 GTTAAC GTT^AAC
KasI 2264 GGCGCC G^GCGCC
KpnI 1472 GGTACC GGTAC^C
KspI 38 CCGCGG CCGC^GG
MaeII 1712 ACGT A^CGT
MluNI 3087 TGGCCA TGG^CCA
MroI 128 TCCGGA T^CCGGA
MscI 3087 TGGCCA TGG^CCA
NarI 2264 GGCGCC GG^CGCC
NdeI 2133 CATATG CA^TATG
PaeR7I 194 CTCGAG C^TCGAG
SacII 38 CCGCGG CCGC^GG
SapI 1299 GCTCTTC GCTCTTC(1/4)
SfiI 1686 GGCCCCATGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 2264 GGCGCC GGC^GCC
SphI 3000 GCATGC GCATG^C
StuI 2106 AGGCCT AGG^CCT
XcmI 2223 CCAGCTCCAAGTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 194 CTCGAG C^TCGAG
XmnI 2914 GAAGACATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AcsI 1342 GAATTC [GA]^AATT[CT]
1754 AAATTT
ApoI 1342 GAATTC [GA]^AATT[CT]
1754 AAATTT
BbsI 2914 GAAGAC GAAGAC(2/6)
1057 GTCTTC
BpuAI 2914 GAAGAC GAAGAC(2/6)
1057 GTCTTC
BsaWI 128 TCCGGA [AT]^CCGG[AT]
188 TCCGGT
BspMI 2372 GCAGGT ACCTGC(4/8)
2509 GCAGGT
BsrBI 22 CCGCTC CCGCTC(-3/-3)
809 CCGCTC
BstBI 833 TTCGAA TT^CGAA
2876 TTCGAA
BstXI 585 CCATCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
726 CCACATGACTGG
EcoNI 1456 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2393 CCTTCACCAGG
HindIII 328 AAGCTT A^AGCTT
2201 AAGCTT
NcoI 566 CCATGG C^CATGG
1690 CCATGG
NheI 81 GCTAGC G^CTAGC
2693 GCTAGC
NspI 854 GCATGT [GA]CATG^[CT]
3000 GCATGC
NspV 833 TTCGAA TT^CGAA
2876 TTCGAA
PflMI 172 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2321 CCATTGGCTGG
PleI 30 GACTC GAGTC(4/5)
968 GAGTC
SacI 121 GAGCTC GAGCT^C
2541 GAGCTC
SfuI 833 TTCGAA TT^CGAA
2876 TTCGAA
Van91I 172 CCAGGAATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2321 CCATTGGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaI AvaII BanI
BanII BbvI BcnI BfaI BlpI
BmyI Bpu1102I BsaHI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
PpuMI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmaI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII
XmaCI XmaI