################################## # NO CUT ENZYMES ( 75 enzymes) # ################################## AatII AclI AflII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvrII BbeI BcgI BclI BfrI BlnI BsiWI BsmBI BspDI BspHI BsrBI BsrDI BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I EcoO65I EcoRI FbaI FseI HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NspV PacI PaeR7I PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SexAI SfiI SfoI SfuI SgrAI SnaBI SpeI SphI SspI SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### Acc65I 1011 GGTACC G^GTACC AccII 1192 CGCG CG^CG AccIII 2702 TCCGGA T^CCGGA Alw44I 827 GTGCAC G^TGCAC AocI 1212 CCTGAGG CC^T[ACGT]AGG ApaLI 827 GTGCAC G^TGCAC Asp700I 2571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 1011 GGTACC G^GTACC AspI 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 851 TGCGCA TGC^GCA BamHI 116 GGATCC G^GATCC BbrPI 97 CACGTG CAC^GTG BbsI 2278 GAAGAC GAAGAC(2/6) BciVI 3651 GGATAC GTATCC(6/5) BglII 980 AGATCT A^GATCT BpuAI 2278 GAAGAC GAAGAC(2/6) BsaAI 97 CACGTG [CT]AC^GT[GA] BsaBI 1114 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsaWI 2702 TCCGGA [AT]^CCGG[AT] BseAI 2702 TCCGGA T^CCGGA BsgI 2427 GTGCAG GTGCAG(16/14) BsiEI 560 CGACCG CG[GA][CT]^CG Bsp1407I 2867 TGTACA T^GTACA BspEI 2702 TCCGGA T^CCGGA BsrFI 1812 GCCGGC [GA]^CCGG[CT] BsrGI 2867 TGTACA T^GTACA BssSI 2477 CACGAG CACGAG(-5/-1) BstUI 1192 CGCG CG^CG Bsu36I 1212 CCTGAGG CC^T[ACGT]AGG Cfr10I 1812 GCCGGC [GA]^CCGG[CT] DraI 3104 TTTAAA TTT^AAA Eco81I 1212 CCTGAGG CC^T[ACGT]AGG EcoRV 2295 GATATC GAT^ATC FspI 851 TGCGCA TGC^GCA HincII 2650 GTCGAC GT[CT]^[GA]AC HindII 2650 GTCGAC GT[CT]^[GA]AC HindIII 2217 AAGCTT A^AGCTT KpnI 1011 GGTACC GGTAC^C MamI 1114 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2702 TCCGGA T^CCGGA MvnI 1192 CGCG CG^CG NaeI 1812 GCCGGC GCC^GGC NgoMIV 1812 GCCGGC G^CCGGC PflFI 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC PflMI 121 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 97 CACGTG CAC^GTG PmlI 97 CACGTG CAC^GTG PshAI 2077 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SalI 2650 GTCGAC G^TCGAC SbfI 3381 CCTGCAGG CCTGCA^GG ScaI 794 AGTACT AGT^ACT SmaI 2061 CCCGGG CCC^GGG Sse8387I 3381 CCTGCAGG CCTGCA^GG SspBI 2867 TGTACA T^GTACA Tth111I 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC Van91I 121 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 2624 CCAAGTCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 2061 CCCGGG C^CCGGG XmaI 2061 CCCGGG C^CCGGG XmnI 2571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 20 enzymes) # ################################### AccI 489 GTCTAC GT^[AC][GT]AC 2650 GTCGAC AcyI 918 GACGCC G[GA]^CG[CT]C 2500 GGCGTC BlpI 538 GCTGAGC GC^T[ACGT]AGC 1488 GCTGAGC Bpu1102I 538 GCTGAGC GC^T[ACGT]AGC 1488 GCTGAGC BsaHI 918 GACGCC G[GA]^CG[CT]C 2500 GGCGTC BsaI 2656 GAGACC GGTCTC(1/5) 2083 GGTCTC BstXI 291 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2490 CCACCACAATGG BstYI 116 GGATCC [GA]^GATC[CT] 980 AGATCT CelII 538 GCTGAGC GC^T[ACGT]AGC 1488 GCTGAGC DsaI 1043 CCATGG C^C[GA][CT]GG 2757 CCATGG EcoT22I 1619 ATGCAT ATGCA^T 3472 ATGCAT Hin1I 918 GACGCC G[GA]^CG[CT]C 2500 GGCGTC MflI 116 GGATCC [GA]^GATC[CT] 980 AGATCT NcoI 1043 CCATGG C^CATGG 2757 CCATGG NsiI 1619 ATGCAT ATGCA^T 3472 ATGCAT PpuMI 968 AGGACCC [GA]G^G[AT]CC[CT] 3040 GGGTCCC SacI 907 GAGCTC GAGCT^C 2841 GAGCTC SapI 1471 GCTCTTC GCTCTTC(1/4) 2574 GCTCTTC StuI 1256 AGGCCT AGG^CCT 3067 AGGCCT XhoII 116 GGATCC [GA]^GATC[CT] 980 AGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AciI AcsI AfaI AflIII AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbvCI BbvI BcnI BfaI BglI BmyI BpmI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsmI BsoBI Bsp1286I BspLU11I BspMI BsrI BssKI BstAPI BstF5I BstNI Cac8I CfoI Cfr13I DdeI DpnII DraII EaeI EarI EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I