##################################
# NO CUT ENZYMES ( 75 enzymes) #
##################################
AatII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI AspEI
AvrII BbeI BcgI BclI BfrI
BlnI BsiWI BsmBI BspDI BspHI
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstZ17I ClaI CpoI
DraIII DrdI EagI Eam1105I EclXI
Eco47III Eco52I EcoO65I EcoRI FbaI
FseI HpaI KasI KspI MfeI
MluI MunI NarI NdeI NheI
NotI NruI NspV PacI PaeR7I
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
Acc65I 1011 GGTACC G^GTACC
AccII 1192 CGCG CG^CG
AccIII 2702 TCCGGA T^CCGGA
Alw44I 827 GTGCAC G^TGCAC
AocI 1212 CCTGAGG CC^T[ACGT]AGG
ApaLI 827 GTGCAC G^TGCAC
Asp700I 2571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1011 GGTACC G^GTACC
AspI 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 851 TGCGCA TGC^GCA
BamHI 116 GGATCC G^GATCC
BbrPI 97 CACGTG CAC^GTG
BbsI 2278 GAAGAC GAAGAC(2/6)
BciVI 3651 GGATAC GTATCC(6/5)
BglII 980 AGATCT A^GATCT
BpuAI 2278 GAAGAC GAAGAC(2/6)
BsaAI 97 CACGTG [CT]AC^GT[GA]
BsaBI 1114 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 2702 TCCGGA [AT]^CCGG[AT]
BseAI 2702 TCCGGA T^CCGGA
BsgI 2427 GTGCAG GTGCAG(16/14)
BsiEI 560 CGACCG CG[GA][CT]^CG
Bsp1407I 2867 TGTACA T^GTACA
BspEI 2702 TCCGGA T^CCGGA
BsrFI 1812 GCCGGC [GA]^CCGG[CT]
BsrGI 2867 TGTACA T^GTACA
BssSI 2477 CACGAG CACGAG(-5/-1)
BstUI 1192 CGCG CG^CG
Bsu36I 1212 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1812 GCCGGC [GA]^CCGG[CT]
DraI 3104 TTTAAA TTT^AAA
Eco81I 1212 CCTGAGG CC^T[ACGT]AGG
EcoRV 2295 GATATC GAT^ATC
FspI 851 TGCGCA TGC^GCA
HincII 2650 GTCGAC GT[CT]^[GA]AC
HindII 2650 GTCGAC GT[CT]^[GA]AC
HindIII 2217 AAGCTT A^AGCTT
KpnI 1011 GGTACC GGTAC^C
MamI 1114 GATTTTAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2702 TCCGGA T^CCGGA
MvnI 1192 CGCG CG^CG
NaeI 1812 GCCGGC GCC^GGC
NgoMIV 1812 GCCGGC G^CCGGC
PflFI 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 121 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 97 CACGTG CAC^GTG
PmlI 97 CACGTG CAC^GTG
PshAI 2077 GACACAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SalI 2650 GTCGAC G^TCGAC
SbfI 3381 CCTGCAGG CCTGCA^GG
ScaI 794 AGTACT AGT^ACT
SmaI 2061 CCCGGG CCC^GGG
Sse8387I 3381 CCTGCAGG CCTGCA^GG
SspBI 2867 TGTACA T^GTACA
Tth111I 2556 GACCAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 121 CCAGAGGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2624 CCAAGTCCAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 2061 CCCGGG C^CCGGG
XmaI 2061 CCCGGG C^CCGGG
XmnI 2571 GAAGCTCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AccI 489 GTCTAC GT^[AC][GT]AC
2650 GTCGAC
AcyI 918 GACGCC G[GA]^CG[CT]C
2500 GGCGTC
BlpI 538 GCTGAGC GC^T[ACGT]AGC
1488 GCTGAGC
Bpu1102I 538 GCTGAGC GC^T[ACGT]AGC
1488 GCTGAGC
BsaHI 918 GACGCC G[GA]^CG[CT]C
2500 GGCGTC
BsaI 2656 GAGACC GGTCTC(1/5)
2083 GGTCTC
BstXI 291 CCAGAAGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2490 CCACCACAATGG
BstYI 116 GGATCC [GA]^GATC[CT]
980 AGATCT
CelII 538 GCTGAGC GC^T[ACGT]AGC
1488 GCTGAGC
DsaI 1043 CCATGG C^C[GA][CT]GG
2757 CCATGG
EcoT22I 1619 ATGCAT ATGCA^T
3472 ATGCAT
Hin1I 918 GACGCC G[GA]^CG[CT]C
2500 GGCGTC
MflI 116 GGATCC [GA]^GATC[CT]
980 AGATCT
NcoI 1043 CCATGG C^CATGG
2757 CCATGG
NsiI 1619 ATGCAT ATGCA^T
3472 ATGCAT
PpuMI 968 AGGACCC [GA]G^G[AT]CC[CT]
3040 GGGTCCC
SacI 907 GAGCTC GAGCT^C
2841 GAGCTC
SapI 1471 GCTCTTC GCTCTTC(1/4)
2574 GCTCTTC
StuI 1256 AGGCCT AGG^CCT
3067 AGGCCT
XhoII 116 GGATCC [GA]^GATC[CT]
980 AGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbvCI BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsmI
BsoBI Bsp1286I BspLU11I BspMI BsrI
BssKI BstAPI BstF5I BstNI Cac8I
CfoI Cfr13I DdeI DpnII DraII
EaeI EarI EcoNI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII HphI ItaI Ksp632I
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MseI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI PvuII RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I