##################################
# NO CUT ENZYMES ( 72 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI AscI AseI AsnI Asp718I
AspEI AspI BciVI BfrI BglII
BsaBI BsaI BsmBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssSI
Bst1107I BstAPI BstEII BstPI BstZ17I
ClaI CpoI Eam1105I EcoO65I EcoRI
EcoRV EcoT22I FseI HindIII HpaI
KpnI MamI MluI NcoI NruI
NsiI PacI PaeR7I PflFI PinAI
PshAI PshBI Psp1406I PvuI RcaI
RsrII SalI SbfI SexAI SfiI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SwaI Tth111I XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccIII 106 TCCGGA T^CCGGA
Alw44I 3099 GTGCAC G^TGCAC
AocI 1359 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1780 AGCGCT AGC^GCT
ApaI 1334 GGGCCC GGGCC^C
ApaLI 3099 GTGCAC G^TGCAC
Asp700I 2447 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 3077 CCCGAG C^[CT]CG[GA]G
AviII 187 TGCGCA TGC^GCA
BamHI 2354 GGATCC G^GATCC
BbrPI 2318 CACGTG CAC^GTG
BbvCI 2491 GCTGAGG CCTCAGC(-5/-2)
BcgI 3 CGACCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2081 TGATCA T^GATCA
BsaAI 2318 CACGTG [CT]AC^GT[GA]
BseAI 106 TCCGGA T^CCGGA
BsiEI 151 CGGCCG CG[GA][CT]^CG
BsiWI 833 CGTACG C^GTACG
BsoBI 3077 CCCGAG C^[CT]CG[GA]G
BspEI 106 TCCGGA T^CCGGA
BsrBI 1304 CCGCTC CCGCTC(-3/-3)
BstBI 2981 TTCGAA TT^CGAA
BstXI 2923 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1359 CCTAAGG CC^T[ACGT]AGG
DraI 2575 TTTAAA TTT^AAA
DrdI 2045 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 151 CGGCCG C^GGCCG
EclXI 151 CGGCCG C^GGCCG
Eco47III 1780 AGCGCT AGC^GCT
Eco52I 151 CGGCCG C^GGCCG
Eco81I 1359 CCTAAGG CC^T[ACGT]AGG
FbaI 2081 TGATCA T^GATCA
FspI 187 TGCGCA TGC^GCA
HincII 2757 GTTGAC GT[CT]^[GA]AC
HindII 2757 GTTGAC GT[CT]^[GA]AC
MfeI 2820 CAATTG C^AATTG
MroI 106 TCCGGA T^CCGGA
MslI 2175 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 2820 CAATTG C^AATTG
NaeI 1229 GCCGGC GCC^GGC
NdeI 2547 CATATG CA^TATG
NgoMIV 1229 GCCGGC G^CCGGC
NheI 2710 GCTAGC G^CTAGC
NotI 150 GCGGCCGC GC^GGCCGC
NspV 2981 TTCGAA TT^CGAA
PmaCI 2318 CACGTG CAC^GTG
PmeI 2574 GTTTAAAC GTTT^AAAC
PmlI 2318 CACGTG CAC^GTG
PpuMI 2016 GGGACCT [GA]G^G[AT]CC[CT]
SfuI 2981 TTCGAA TT^CGAA
SspI 2722 AATATT AAT^ATT
StuI 2002 AGGCCT AGG^CCT
XmnI 2447 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 2091 GTAGAC GT^[AC][GT]AC
3031 GTAGAC
AflIII 696 ACACGT A^C[GA][CT]GT
2317 ACACGT
AvrII 1349 CCTAGG C^CTAGG
1384 CCTAGG
BbeI 67 GGCGCC GGCGC^C
776 GGCGCC
BlnI 1349 CCTAGG C^CTAGG
1384 CCTAGG
BsaWI 106 TCCGGA [AT]^CCGG[AT]
1815 TCCGGT
BsmI 2447 GAATGC GAATGC(1/-1)
1233 GCATTC
BspMI 2436 ACCTGC ACCTGC(4/8)
961 GCAGGT
BssHII 165 GCGCGC G^CGCGC
866 GCGCGC
BstYI 2354 GGATCC [GA]^GATC[CT]
2515 GGATCT
DraII 1333 GGGGCCC [GA]G^G[ACGT]CC[CT]
2016 GGGACCT
DraIII 200 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG
629 CACCCTGTG
EcoNI 211 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1324 CCTCTGGGAGG
EcoO109I 1333 GGGGCCC [GA]G^G[ACGT]CC[CT]
2016 GGGACCT
HphI 2079 GGTGA GGTGA(8/7)
450 TCACC
KasI 67 GGCGCC G^GCGCC
776 GGCGCC
MflI 2354 GGATCC [GA]^GATC[CT]
2515 GGATCT
NarI 67 GGCGCC GG^CGCC
776 GGCGCC
PstI 566 CTGCAG CTGCA^G
2210 CTGCAG
SacI 131 GAGCTC GAGCT^C
810 GAGCTC
SapI 133 GCTCTTC GCTCTTC(1/4)
2394 GCTCTTC
ScaI 412 AGTACT AGT^ACT
1143 AGTACT
SfoI 67 GGCGCC GGC^GCC
776 GGCGCC
SmlI 332 CTCAAG C^T[CT][GA]AG
349 CTCAAG
XbaI 2443 TCTAGA T^CTAGA
2843 TCTAGA
XhoII 2354 GGATCC [GA]^GATC[CT]
2515 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 107 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI AlwNI ApoI AspHI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BsrFI
BsrI BssKI BstF5I BstNI BstUI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DsaI EaeI EarI
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HinfI HpaII ItaI
Ksp632I KspI MaeI MaeII MaeIII
MboI MboII MluNI MnlI MscI
MseI MspA1I MspI MvaI MvnI
MwoI NciI NdeII NlaIII NlaIV
NspI PflMI PleI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI