################################## # NO CUT ENZYMES ( 72 enzymes) # ################################## AatII Acc65I AclI AflII AgeI AhdI AscI AseI AsnI Asp718I AspEI AspI BciVI BfrI BglII BsaBI BsaI BsmBI Bsp1407I BspDI BspHI BspLU11I BsrDI BsrGI BssSI Bst1107I BstAPI BstEII BstPI BstZ17I ClaI CpoI Eam1105I EcoO65I EcoRI EcoRV EcoT22I FseI HindIII HpaI KpnI MamI MluI NcoI NruI NsiI PacI PaeR7I PflFI PinAI PshAI PshBI Psp1406I PvuI RcaI RsrII SalI SbfI SexAI SfiI SgrAI SmaI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XhoI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 53 enzymes) # ################################### AccIII 106 TCCGGA T^CCGGA Alw44I 3099 GTGCAC G^TGCAC AocI 1359 CCTAAGG CC^T[ACGT]AGG Aor51HI 1780 AGCGCT AGC^GCT ApaI 1334 GGGCCC GGGCC^C ApaLI 3099 GTGCAC G^TGCAC Asp700I 2447 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC AvaI 3077 CCCGAG C^[CT]CG[GA]G AviII 187 TGCGCA TGC^GCA BamHI 2354 GGATCC G^GATCC BbrPI 2318 CACGTG CAC^GTG BbvCI 2491 GCTGAGG CCTCAGC(-5/-2) BcgI 3 CGACCACACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2081 TGATCA T^GATCA BsaAI 2318 CACGTG [CT]AC^GT[GA] BseAI 106 TCCGGA T^CCGGA BsiEI 151 CGGCCG CG[GA][CT]^CG BsiWI 833 CGTACG C^GTACG BsoBI 3077 CCCGAG C^[CT]CG[GA]G BspEI 106 TCCGGA T^CCGGA BsrBI 1304 CCGCTC CCGCTC(-3/-3) BstBI 2981 TTCGAA TT^CGAA BstXI 2923 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1359 CCTAAGG CC^T[ACGT]AGG DraI 2575 TTTAAA TTT^AAA DrdI 2045 GACATCCGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 151 CGGCCG C^GGCCG EclXI 151 CGGCCG C^GGCCG Eco47III 1780 AGCGCT AGC^GCT Eco52I 151 CGGCCG C^GGCCG Eco81I 1359 CCTAAGG CC^T[ACGT]AGG FbaI 2081 TGATCA T^GATCA FspI 187 TGCGCA TGC^GCA HincII 2757 GTTGAC GT[CT]^[GA]AC HindII 2757 GTTGAC GT[CT]^[GA]AC MfeI 2820 CAATTG C^AATTG MroI 106 TCCGGA T^CCGGA MslI 2175 CATTCACATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 2820 CAATTG C^AATTG NaeI 1229 GCCGGC GCC^GGC NdeI 2547 CATATG CA^TATG NgoMIV 1229 GCCGGC G^CCGGC NheI 2710 GCTAGC G^CTAGC NotI 150 GCGGCCGC GC^GGCCGC NspV 2981 TTCGAA TT^CGAA PmaCI 2318 CACGTG CAC^GTG PmeI 2574 GTTTAAAC GTTT^AAAC PmlI 2318 CACGTG CAC^GTG PpuMI 2016 GGGACCT [GA]G^G[AT]CC[CT] SfuI 2981 TTCGAA TT^CGAA SspI 2722 AATATT AAT^ATT StuI 2002 AGGCCT AGG^CCT XmnI 2447 GAATGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AccI 2091 GTAGAC GT^[AC][GT]AC 3031 GTAGAC AflIII 696 ACACGT A^C[GA][CT]GT 2317 ACACGT AvrII 1349 CCTAGG C^CTAGG 1384 CCTAGG BbeI 67 GGCGCC GGCGC^C 776 GGCGCC BlnI 1349 CCTAGG C^CTAGG 1384 CCTAGG BsaWI 106 TCCGGA [AT]^CCGG[AT] 1815 TCCGGT BsmI 2447 GAATGC GAATGC(1/-1) 1233 GCATTC BspMI 2436 ACCTGC ACCTGC(4/8) 961 GCAGGT BssHII 165 GCGCGC G^CGCGC 866 GCGCGC BstYI 2354 GGATCC [GA]^GATC[CT] 2515 GGATCT DraII 1333 GGGGCCC [GA]G^G[ACGT]CC[CT] 2016 GGGACCT DraIII 200 CACCCGGTG CAC[ACGT][ACGT][ACGT]^GTG 629 CACCCTGTG EcoNI 211 CCTGGCCGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 1324 CCTCTGGGAGG EcoO109I 1333 GGGGCCC [GA]G^G[ACGT]CC[CT] 2016 GGGACCT HphI 2079 GGTGA GGTGA(8/7) 450 TCACC KasI 67 GGCGCC G^GCGCC 776 GGCGCC MflI 2354 GGATCC [GA]^GATC[CT] 2515 GGATCT NarI 67 GGCGCC GG^CGCC 776 GGCGCC PstI 566 CTGCAG CTGCA^G 2210 CTGCAG SacI 131 GAGCTC GAGCT^C 810 GAGCTC SapI 133 GCTCTTC GCTCTTC(1/4) 2394 GCTCTTC ScaI 412 AGTACT AGT^ACT 1143 AGTACT SfoI 67 GGCGCC GGC^GCC 776 GGCGCC SmlI 332 CTCAAG C^T[CT][GA]AG 349 CTCAAG XbaI 2443 TCTAGA T^CTAGA 2843 TCTAGA XhoII 2354 GGATCC [GA]^GATC[CT] 2515 GGATCT ######################################### # 3 OR MORE CUT ENZYMES ( 107 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI AlwNI ApoI AspHI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaHI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DsaI EaeI EarI EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII ItaI Ksp632I KspI MaeI MaeII MaeIII MboI MboII MluNI MnlI MscI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI