##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AclI AcyI AgeI AscI
AseI AsnI Asp700I AspI AvrII
BamHI BbeI BbsI BbvCI BcgI
BlnI BpuAI BsaBI BsaHI BsiWI
Bsp1407I BspDI BspLU11I BsrDI BsrGI
BssHII BssSI Bst1107I BstBI BstZ17I
ClaI CpoI DraI EcoT22I FseI
Hin1I HpaI KasI MamI MluI
NaeI NarI NdeI NgoMIV NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI Psp1406I
PvuI PvuII RsrII SacI SapI
ScaI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 39 enzymes) #
###################################
AccI 1813 GTCGAC GT^[AC][GT]AC
AflII 1680 CTTAAG C^TTAAG
AflIII 3101 ACACGT A^C[GA][CT]GT
AhdI 459 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Aor51HI 1239 AGCGCT AGC^GCT
AspEI 459 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1591 TGCGCA TGC^GCA
BbrPI 3102 CACGTG CAC^GTG
BclI 391 TGATCA T^GATCA
BfrI 1680 CTTAAG C^TTAAG
BglII 1856 AGATCT A^GATCT
BsaAI 3102 CACGTG [CT]AC^GT[GA]
BspHI 1009 TCATGA T^CATGA
BsrBI 1124 CCGCTC CCGCTC(-3/-3)
BsrFI 3222 ACCGGC [GA]^CCGG[CT]
Cfr10I 3222 ACCGGC [GA]^CCGG[CT]
DrdI 2043 GACCACACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 459 GACGAGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1239 AGCGCT AGC^GCT
EcoRI 2247 GAATTC G^AATTC
EcoRV 2624 GATATC GAT^ATC
FbaI 391 TGATCA T^GATCA
FspI 1591 TGCGCA TGC^GCA
HindIII 879 AAGCTT A^AGCTT
KspI 74 CCGCGG CCGC^GG
MfeI 3274 CAATTG C^AATTG
MunI 3274 CAATTG C^AATTG
NheI 3052 GCTAGC G^CTAGC
NotI 70 GCGGCCGC GC^GGCCGC
PmaCI 3102 CACGTG CAC^GTG
PmlI 3102 CACGTG CAC^GTG
RcaI 1009 TCATGA T^CATGA
SacII 74 CCGCGG CCGC^GG
SalI 1813 GTCGAC G^TCGAC
SbfI 1481 CCTGCAGG CCTGCA^GG
SphI 3245 GCATGC GCATG^C
Sse8387I 1481 CCTGCAGG CCTGCA^GG
StuI 1444 AGGCCT AGG^CCT
XbaI 1859 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 37 enzymes) #
###################################
Acc65I 1990 GGTACC G^GTACC
3354 GGTACC
AccIII 547 TCCGGA T^CCGGA
1285 TCCGGA
AcsI 2247 GAATTC [GA]^AATT[CT]
3669 AAATTC
Alw44I 2272 GTGCAC G^TGCAC
3187 GTGCAC
ApaI 1825 GGGCCC GGGCC^C
2334 GGGCCC
ApaLI 2272 GTGCAC G^TGCAC
3187 GTGCAC
ApoI 2247 GAATTC [GA]^AATT[CT]
3669 AAATTC
Asp718I 1990 GGTACC G^GTACC
3354 GGTACC
BglI 1951 GCCACGGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2740 GCCAAGAGGGC
BlpI 876 GCTAAGC GC^T[ACGT]AGC
3563 GCTGAGC
Bpu1102I 876 GCTAAGC GC^T[ACGT]AGC
3563 GCTGAGC
BseAI 547 TCCGGA T^CCGGA
1285 TCCGGA
BsiEI 71 CGGCCG CG[GA][CT]^CG
77 CGGCCG
BsmBI 1760 CGTCTC CGTCTC(1/5)
451 GAGACG
BsmI 179 GAATGC GAATGC(1/-1)
2094 GAATGC
BspEI 547 TCCGGA T^CCGGA
1285 TCCGGA
BstAPI 1424 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2431 GCATCCCCTGC
BstEII 2788 GGTGACC G^GT[ACGT]ACC
2874 GGTTACC
BstPI 2788 GGTGACC G^GT[ACGT]ACC
2874 GGTTACC
CelII 876 GCTAAGC GC^T[ACGT]AGC
3563 GCTGAGC
EagI 71 CGGCCG C^GGCCG
77 CGGCCG
EclXI 71 CGGCCG C^GGCCG
77 CGGCCG
Eco52I 71 CGGCCG C^GGCCG
77 CGGCCG
EcoO65I 2788 GGTGACC G^GT[ACGT]ACC
2874 GGTTACC
FauI 33 CCCGC CCCGC(4/6)
571 CCCGC
HincII 1813 GTCGAC GT[CT]^[GA]AC
3324 GTCAAC
HindII 1813 GTCGAC GT[CT]^[GA]AC
3324 GTCAAC
KpnI 1990 GGTACC GGTAC^C
3354 GGTACC
MroI 547 TCCGGA T^CCGGA
1285 TCCGGA
MspA1I 74 CCGCGG C[AC]G^C[GT]G
1635 CAGCGG
NspI 682 GCATGT [GA]CATG^[CT]
3245 GCATGC
PflMI 1036 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1974 CCATTGTTTGG
SmaI 20 CCCGGG CCC^GGG
3571 CCCGGG
TfiI 188 GATTC G^A[AT]TC
2222 GAATC
Van91I 1036 CCAGAGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1974 CCATTGTTTGG
XmaCI 20 CCCGGG C^CCGGG
3571 CCCGGG
XmaI 20 CCCGGG C^CCGGG
3571 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 108 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI AocI AspHI AvaI AvaII
BalI BanI BanII BbvI BciVI
BcnI BfaI BmyI BpmI BsaI
BsaJI BsaWI BseRI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrI BssKI BstF5I
BstNI BstUI BstXI BstYI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EarI
Eco81I EcoNI EcoO109I EcoRII EcoT14I
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MluNI
MnlI MscI MseI MslI MspI
MvaI MvnI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI PpuMI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TaqI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII