##################################
# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII AccII AccIII AclI AcyI
AgeI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I AvrII BamHI
BbeI BbrPI BcgI BclI BlnI
BsaBI BsaHI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspHI
BsrBI BsrFI BssHII BstAPI BstBI
BstUI Cfr10I ClaI CpoI DrdI
EagI EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI HgaI
Hin1I HpaI KasI KspI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI SwaI XcmI XhoI
XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 30 enzymes) #
###################################
Acc65I 873 GGTACC G^GTACC
AccI 708 GTATAC GT^[AC][GT]AC
AhdI 1073 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 873 GGTACC G^GTACC
AspEI 1073 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2833 TGCGCA TGC^GCA
BciVI 422 GTATCC GTATCC(6/5)
BsaAI 2103 TACGTG [CT]AC^GT[GA]
BsaI 3561 GGTCTC GGTCTC(1/5)
Bsp1407I 3673 TGTACA T^GTACA
BsrGI 3673 TGTACA T^GTACA
Bst1107I 708 GTATAC GTA^TAC
BstZ17I 708 GTATAC GTA^TAC
DraI 3774 TTTAAA TTT^AAA
Eam1105I 1073 GACCTGATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 3596 CCTGACAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FspI 2833 TGCGCA TGC^GCA
HincII 824 GTTGAC GT[CT]^[GA]AC
HindII 824 GTTGAC GT[CT]^[GA]AC
KpnI 873 GGTACC GGTAC^C
NcoI 3554 CCATGG C^CATGG
NdeI 1200 CATATG CA^TATG
PflFI 635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
SapI 668 GAAGAGC GCTCTTC(1/4)
ScaI 213 AGTACT AGT^ACT
SfiI 2639 GGCCCACGAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SspBI 3673 TGTACA T^GTACA
Tth111I 635 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1973 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AflII 1 CTTAAG C^TTAAG
3412 CTTAAG
AlwNI 713 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
2165 CAGCTGCTG
BalI 2254 TGGCCA TGG^CCA
2856 TGGCCA
BanI 278 GGCACC G^G[CT][GA]CC
873 GGTACC
BcnI 1663 CCGGG CC^[GC]GG
2759 CCCGG
BfrI 1 CTTAAG C^TTAAG
3412 CTTAAG
BglI 2256 GCCAGGTCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2640 GCCCACGAGGC
BspMI 1686 ACCTGC ACCTGC(4/8)
3049 ACCTGC
BstEII 2206 GGTGACC G^GT[ACGT]ACC
2762 GGTGACC
BstPI 2206 GGTGACC G^GT[ACGT]ACC
2762 GGTGACC
BstXI 1489 CCATCTCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2245 CCAGCCACCTGG
EcoO65I 2206 GGTGACC G^GT[ACGT]ACC
2762 GGTGACC
HindIII 400 AAGCTT A^AGCTT
3409 AAGCTT
MluNI 2254 TGGCCA TGG^CCA
2856 TGGCCA
MscI 2254 TGGCCA TGG^CCA
2856 TGGCCA
NciI 1663 CCGGG CC^[GC]GG
2759 CCCGG
SphI 808 GCATGC GCATG^C
2836 GCATGC
StuI 100 AGGCCT AGG^CCT
2612 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 112 enzymes) #
#########################################
AciI AcsI AfaI AflIII AluI
AlwI AocI ApaI ApoI AspHI
AvaI AvaII BanII BbsI BbvCI
BbvI BfaI BglII BlpI BmyI
BpmI Bpu1102I BpuAI BsaJI BsaWI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BsrDI BsrI BssKI BssSI BstF5I
BstNI BstYI Bsu36I Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
DraIII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MslI
MspA1I MspI MvaI MwoI NdeII
NlaIII NlaIV NspI PflMI PleI
PpuMI PstI PvuII RsaI SacI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII