################################## # NO CUT ENZYMES ( 102 enzymes) # ################################## AatII Acc65I AccII AccIII AclI AcyI AgeI Alw44I Aor51HI ApaLI AscI AseI AsnI Asp700I Asp718I AviII AvrII BbeI BbrPI BcgI BciVI BclI BglII BlnI BsaAI BsaHI BsaWI BseAI BsiEI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BsrFI BsrGI BssHII Bst1107I BstAPI BstBI BstUI BstZ17I Cfr10I ClaI CpoI DrdI DsaI EagI EclXI Eco47III Eco52I EcoRI EcoRV EcoT22I FbaI FseI FspI Hin1I KasI KpnI KspI MfeI MluI MroI MunI MvnI NaeI NarI NcoI NgoMIV NheI NotI NruI NsiI NspV PacI PinAI PmaCI PmeI PmlI PshAI PshBI Psp1406I PvuI RsrII SacI SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SnaBI SpeI Sse8387I SspBI SspI XbaI XmnI ################################### # ONE CUT ENZYMES ( 34 enzymes) # ################################### AflIII 2166 ACATGT A^C[GA][CT]GT ApaI 2020 GGGCCC GGGCC^C AspI 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC BalI 1027 TGGCCA TGG^CCA BamHI 1748 GGATCC G^GATCC BanI 566 GGCACC G^G[CT][GA]CC BlpI 1434 GCTAAGC GC^T[ACGT]AGC Bpu1102I 1434 GCTAAGC GC^T[ACGT]AGC BsaI 1635 GAGACC GGTCTC(1/5) BspHI 1987 TCATGA T^CATGA BspLU11I 2166 ACATGT A^CATGT BspMI 2330 ACCTGC ACCTGC(4/8) BsrBI 2252 CCGCTC CCGCTC(-3/-3) BstEII 2001 GGTGACC G^GT[ACGT]ACC BstPI 2001 GGTGACC G^GT[ACGT]ACC CelII 1434 GCTAAGC GC^T[ACGT]AGC DraIII 433 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG EcoO65I 2001 GGTGACC G^GT[ACGT]ACC FauI 2108 CCCGC CCCGC(4/6) HgaI 2216 GCGTC GACGC(5/10) HpaI 2984 GTTAAC GTT^AAC MluNI 1027 TGGCCA TGG^CCA MscI 1027 TGGCCA TGG^CCA NdeI 2698 CATATG CA^TATG PflFI 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC PstI 907 CTGCAG CTGCA^G RcaI 1987 TCATGA T^CATGA SapI 1578 GAAGAGC GCTCTTC(1/4) SexAI 1774 ACCTGGT A^CC[AT]GGT SphI 2212 GCATGC GCATG^C SwaI 3031 ATTTAAAT ATTT^AAAT TfiI 1221 GATTC G^A[AT]TC Tth111I 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC XcmI 2495 CCAGACCTGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 111 GTAGAC GT^[AC][GT]AC 1734 GTAGAC AflII 1869 CTTAAG C^TTAAG 2950 CTTAAG AhdI 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1866 GACCTTAAGTC AlwNI 991 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG 1483 CAGAGACTG AocI 628 CCTTAGG CC^T[ACGT]AGG 1128 CCTCAGG AspEI 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1866 GACCTTAAGTC BfrI 1869 CTTAAG C^TTAAG 2950 CTTAAG BsaBI 127 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1743 GATATGGATC BssSI 342 CACGAG CACGAG(-5/-1) 601 CACGAG Bsu36I 628 CCTTAGG CC^T[ACGT]AGG 1128 CCTCAGG CfoI 311 GCGC GCG^C 2690 GCGC EaeI 798 CGGCCA [CT]^GGCC[GA] 1027 TGGCCA Eam1105I 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC 1866 GACCTTAAGTC Eco81I 628 CCTTAGG CC^T[ACGT]AGG 1128 CCTCAGG HaeII 310 GGCGCT [GA]GCGC^[CT] 2689 AGCGCC HhaI 311 GCGC GCG^C 2690 GCGC HinP1I 311 GCGC G^CGC 2690 GCGC HindIII 2383 AAGCTT A^AGCTT 3009 AAGCTT MamI 127 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1743 GATATGGATC MslI 44 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 1806 CACAAGCATG PaeR7I 899 CTCGAG C^TCGAG 2395 CTCGAG SmaI 148 CCCGGG CCC^GGG 2452 CCCGGG XhoI 899 CTCGAG C^TCGAG 2395 CTCGAG XmaCI 148 CCCGGG C^CCGGG 2452 CCCGGG XmaI 148 CCCGGG C^CCGGG 2452 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AciI AcsI AfaI AluI AlwI ApoI AspHI AvaI AvaII BanII BbsI BbvCI BbvI BcnI BfaI BglI BmyI BpmI BpuAI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrDI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr13I DdeI DpnII DraI DraII EarI EcoNI EcoO109I EcoRII EcoT14I Fnu4HI FokI HaeIII HapII HincII HindII HinfI HpaII HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PflMI PleI PpuMI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoII