##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccII AccIII AclI
AcyI AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp700I Asp718I
AviII AvrII BbeI BbrPI BcgI
BciVI BclI BglII BlnI BsaAI
BsaHI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspDI BspEI
BsrFI BsrGI BssHII Bst1107I BstAPI
BstBI BstUI BstZ17I Cfr10I ClaI
CpoI DrdI DsaI EagI EclXI
Eco47III Eco52I EcoRI EcoRV EcoT22I
FbaI FseI FspI Hin1I KasI
KpnI KspI MfeI MluI MroI
MunI MvnI NaeI NarI NcoI
NgoMIV NheI NotI NruI NsiI
NspV PacI PinAI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfoI SfuI SgrAI
SnaBI SpeI Sse8387I SspBI SspI
XbaI XmnI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
AflIII 2166 ACATGT A^C[GA][CT]GT
ApaI 2020 GGGCCC GGGCC^C
AspI 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 1027 TGGCCA TGG^CCA
BamHI 1748 GGATCC G^GATCC
BanI 566 GGCACC G^G[CT][GA]CC
BlpI 1434 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 1434 GCTAAGC GC^T[ACGT]AGC
BsaI 1635 GAGACC GGTCTC(1/5)
BspHI 1987 TCATGA T^CATGA
BspLU11I 2166 ACATGT A^CATGT
BspMI 2330 ACCTGC ACCTGC(4/8)
BsrBI 2252 CCGCTC CCGCTC(-3/-3)
BstEII 2001 GGTGACC G^GT[ACGT]ACC
BstPI 2001 GGTGACC G^GT[ACGT]ACC
CelII 1434 GCTAAGC GC^T[ACGT]AGC
DraIII 433 CACTGAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO65I 2001 GGTGACC G^GT[ACGT]ACC
FauI 2108 CCCGC CCCGC(4/6)
HgaI 2216 GCGTC GACGC(5/10)
HpaI 2984 GTTAAC GTT^AAC
MluNI 1027 TGGCCA TGG^CCA
MscI 1027 TGGCCA TGG^CCA
NdeI 2698 CATATG CA^TATG
PflFI 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 907 CTGCAG CTGCA^G
RcaI 1987 TCATGA T^CATGA
SapI 1578 GAAGAGC GCTCTTC(1/4)
SexAI 1774 ACCTGGT A^CC[AT]GGT
SphI 2212 GCATGC GCATG^C
SwaI 3031 ATTTAAAT ATTT^AAAT
TfiI 1221 GATTC G^A[AT]TC
Tth111I 2468 GACCGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2495 CCAGACCTGTACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 111 GTAGAC GT^[AC][GT]AC
1734 GTAGAC
AflII 1869 CTTAAG C^TTAAG
2950 CTTAAG
AhdI 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1866 GACCTTAAGTC
AlwNI 991 CAGAGCCTG CAG[ACGT][ACGT][ACGT]^CTG
1483 CAGAGACTG
AocI 628 CCTTAGG CC^T[ACGT]AGG
1128 CCTCAGG
AspEI 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1866 GACCTTAAGTC
BfrI 1869 CTTAAG C^TTAAG
2950 CTTAAG
BsaBI 127 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1743 GATATGGATC
BssSI 342 CACGAG CACGAG(-5/-1)
601 CACGAG
Bsu36I 628 CCTTAGG CC^T[ACGT]AGG
1128 CCTCAGG
CfoI 311 GCGC GCG^C
2690 GCGC
EaeI 798 CGGCCA [CT]^GGCC[GA]
1027 TGGCCA
Eam1105I 933 GACTCCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1866 GACCTTAAGTC
Eco81I 628 CCTTAGG CC^T[ACGT]AGG
1128 CCTCAGG
HaeII 310 GGCGCT [GA]GCGC^[CT]
2689 AGCGCC
HhaI 311 GCGC GCG^C
2690 GCGC
HinP1I 311 GCGC G^CGC
2690 GCGC
HindIII 2383 AAGCTT A^AGCTT
3009 AAGCTT
MamI 127 GATGGAAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1743 GATATGGATC
MslI 44 CATTGTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1806 CACAAGCATG
PaeR7I 899 CTCGAG C^TCGAG
2395 CTCGAG
SmaI 148 CCCGGG CCC^GGG
2452 CCCGGG
XhoI 899 CTCGAG C^TCGAG
2395 CTCGAG
XmaCI 148 CCCGGG C^CCGGG
2452 CCCGGG
XmaI 148 CCCGGG C^CCGGG
2452 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AspHI AvaI AvaII BanII
BbsI BbvCI BbvI BcnI BfaI
BglI BmyI BpmI BpuAI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BsrDI
BsrI BssKI BstF5I BstNI BstXI
BstYI Cac8I Cfr13I DdeI DpnII
DraI DraII EarI EcoNI EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MspI MvaI MwoI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmlI StuI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoII