##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AccIII AclI AflII AgeI AhdI
Aor51HI AscI AseI AsnI Asp700I
AspEI AviII BbeI BbvCI BciVI
BfrI BglII BsaBI BsaWI BseAI
BsiWI BsmI Bsp1407I BspDI BspEI
BsrBI BsrGI BssHII BstBI BstEII
BstPI ClaI CpoI Eam1105I Eco47III
EcoO65I EcoRI EcoRV FseI FspI
HaeII HgaI KasI MamI MfeI
MluI MroI MunI NarI NheI
NotI NruI NspV PacI PaeR7I
PflMI PinAI PmeI PshAI PshBI
Psp1406I PvuI RsrII SalI ScaI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI SspBI
SwaI TaqI TthHB8I Van91I XbaI
XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 2298 GACGTC GACGT^C
Acc65I 2622 GGTACC G^GTACC
AccII 1288 CGCG CG^CG
AcsI 705 AAATTC [GA]^AATT[CT]
AcyI 2298 GACGTC G[GA]^CG[CT]C
AocI 813 CCTCAGG CC^T[ACGT]AGG
ApoI 705 AAATTC [GA]^AATT[CT]
Asp718I 2622 GGTACC G^GTACC
AspI 2610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 3018 CCCGAG C^[CT]CG[GA]G
AvrII 2816 CCTAGG C^CTAGG
BalI 1796 TGGCCA TGG^CCA
BamHI 1088 GGATCC G^GATCC
BcgI 982 GCAGTGATGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2816 CCTAGG C^CTAGG
BsaHI 2298 GACGTC G[GA]^CG[CT]C
BsaI 2997 GAGACC GGTCTC(1/5)
BsiEI 2791 CGGCCG CG[GA][CT]^CG
BsmBI 2594 CGTCTC CGTCTC(1/5)
BsoBI 3018 CCCGAG C^[CT]CG[GA]G
BspHI 1850 TCATGA T^CATGA
BsrFI 3008 GCCGGC [GA]^CCGG[CT]
BssSI 473 CACGAG CACGAG(-5/-1)
Bst1107I 3290 GTATAC GTA^TAC
BstUI 1288 CGCG CG^CG
BstYI 1088 GGATCC [GA]^GATC[CT]
BstZ17I 3290 GTATAC GTA^TAC
Bsu36I 813 CCTCAGG CC^T[ACGT]AGG
Cfr10I 3008 GCCGGC [GA]^CCGG[CT]
DraI 3198 TTTAAA TTT^AAA
DrdI 2486 GACTGACCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2791 CGGCCG C^GGCCG
EclXI 2791 CGGCCG C^GGCCG
Eco52I 2791 CGGCCG C^GGCCG
Eco81I 813 CCTCAGG CC^T[ACGT]AGG
EcoT22I 759 ATGCAT ATGCA^T
Hin1I 2298 GACGTC G[GA]^CG[CT]C
HindIII 375 AAGCTT A^AGCTT
HpaI 2325 GTTAAC GTT^AAC
KpnI 2622 GGTACC GGTAC^C
KspI 1287 CCGCGG CCGC^GG
MflI 1088 GGATCC [GA]^GATC[CT]
MluNI 1796 TGGCCA TGG^CCA
MscI 1796 TGGCCA TGG^CCA
MvnI 1288 CGCG CG^CG
NaeI 3008 GCCGGC GCC^GGC
NdeI 3433 CATATG CA^TATG
NgoMIV 3008 GCCGGC G^CCGGC
NsiI 759 ATGCAT ATGCA^T
PflFI 2610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 3093 AGGTCCT [GA]G^G[AT]CC[CT]
RcaI 1850 TCATGA T^CATGA
SacII 1287 CCGCGG CCGC^GG
SapI 2710 GAAGAGC GCTCTTC(1/4)
SspI 1131 AATATT AAT^ATT
Tth111I 2610 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1088 GGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 2259 GTAGAC GT^[AC][GT]AC
3290 GTATAC
Alw44I 29 GTGCAC G^TGCAC
1265 GTGCAC
AlwNI 444 CAGCTACTG CAG[ACGT][ACGT][ACGT]^CTG
1257 CAGAACCTG
ApaI 42 GGGCCC GGGCC^C
1877 GGGCCC
ApaLI 29 GTGCAC G^TGCAC
1265 GTGCAC
BclI 469 TGATCA T^GATCA
1376 TGATCA
BfaI 2638 CTAG C^TAG
2817 CTAG
BlpI 2176 GCTCAGC GC^T[ACGT]AGC
2452 GCTCAGC
Bpu1102I 2176 GCTCAGC GC^T[ACGT]AGC
2452 GCTCAGC
BseRI 2782 CTCCTC GAGGAG(10/8)
3302 CTCCTC
BsrDI 2735 CATTGC GCAATG(2/0)
2371 GCAATG
BstXI 1999 CCAACTGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2695 CCAGGTTCGTGG
CelII 2176 GCTCAGC GC^T[ACGT]AGC
2452 GCTCAGC
CfoI 2836 GCGC GCG^C
3478 GCGC
DraIII 426 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
1346 CACACCGTG
EarI 777 CTCTTC CTCTTC(1/4)
2710 GAAGAG
FbaI 469 TGATCA T^GATCA
1376 TGATCA
HhaI 2836 GCGC GCG^C
3478 GCGC
HinP1I 2836 GCGC G^CGC
3478 GCGC
Ksp632I 777 CTCTTC CTCTTC(1/4)
2710 GAAGAG
MaeI 2638 CTAG C^TAG
2817 CTAG
NcoI 692 CCATGG C^CATGG
898 CCATGG
SbfI 944 CCTGCAGG CCTGCA^GG
1864 CCTGCAGG
Sse8387I 944 CCTGCAGG CCTGCA^GG
1864 CCTGCAGG
StuI 1324 AGGCCT AGG^CCT
2098 AGGCCT
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AspHI AvaII BanI BanII BbrPI
BbsI BbvI BcnI BglI BmyI
BpmI BpuAI BsaAI BsaJI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspLU11I BspMI BsrI BssKI
BstAPI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
NspI PleI PmaCI PmlI PstI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI SphI StyI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI XcmI