################################## # NO CUT ENZYMES ( 70 enzymes) # ################################## AatII AhdI Aor51HI ApaI AscI Asp700I AspEI AspI AviII AvrII BamHI BbvCI BlnI BsaI BseRI BsiWI BspDI BspLU11I BssHII Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraII DraIII Eam1105I EarI Eco47III EcoNI EcoO109I EcoO65I FauI FseI FspI HindIII HpaI Ksp632I MluI NheI NotI NruI NspV PacI PaeR7I PflFI PmeI PpuMI PshAI PvuI PvuII RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SnaBI SpeI Sse8387I StuI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 61 enzymes) # ################################### Acc65I 3622 GGTACC G^GTACC AccIII 1891 TCCGGA T^CCGGA AclI 229 AACGTT AA^CGTT AflIII 2255 ACGTGT A^C[GA][CT]GT AgeI 323 ACCGGT A^CCGGT Alw44I 4043 GTGCAC G^TGCAC AlwNI 1530 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG AocI 4337 CCTAAGG CC^T[ACGT]AGG ApaLI 4043 GTGCAC G^TGCAC Asp718I 3622 GGTACC G^GTACC BalI 1191 TGGCCA TGG^CCA BbrPI 2254 CACGTG CAC^GTG BcgI 2037 GCAAATGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BciVI 3777 GTATCC GTATCC(6/5) BclI 888 TGATCA T^GATCA BlpI 1278 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1278 GCTCAGC GC^T[ACGT]AGC BsaAI 2254 CACGTG [CT]AC^GT[GA] BseAI 1891 TCCGGA T^CCGGA BsiEI 180 CGGCCG CG[GA][CT]^CG BsmBI 3268 GAGACG CGTCTC(1/5) BsmFI 2465 GGGAC GGGAC(10/14) BsmI 2944 GAATGC GAATGC(1/-1) BspEI 1891 TCCGGA T^CCGGA BspHI 3220 TCATGA T^CATGA BspMI 1521 ACCTGC ACCTGC(4/8) BsrBI 2321 GAGCGG CCGCTC(-3/-3) BsrDI 4096 CATTGC GCAATG(2/0) BssSI 1844 CACGAG CACGAG(-5/-1) BstXI 1140 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 4337 CCTAAGG CC^T[ACGT]AGG CelII 1278 GCTCAGC GC^T[ACGT]AGC DrdI 2143 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 180 CGGCCG C^GGCCG EclXI 180 CGGCCG C^GGCCG Eco52I 180 CGGCCG C^GGCCG Eco81I 4337 CCTAAGG CC^T[ACGT]AGG EcoRI 1796 GAATTC G^AATTC EcoRV 2730 GATATC GAT^ATC EcoT22I 2041 ATGCAT ATGCA^T FbaI 888 TGATCA T^GATCA HincII 308 GTCAAC GT[CT]^[GA]AC HindII 308 GTCAAC GT[CT]^[GA]AC KpnI 3622 GGTACC GGTAC^C KspI 72 CCGCGG CCGC^GG MluNI 1191 TGGCCA TGG^CCA MroI 1891 TCCGGA T^CCGGA MscI 1191 TGGCCA TGG^CCA NcoI 282 CCATGG C^CATGG NsiI 2041 ATGCAT ATGCA^T NspI 1874 GCATGC [GA]CATG^[CT] PinAI 323 ACCGGT A^CCGGT PmaCI 2254 CACGTG CAC^GTG PmlI 2254 CACGTG CAC^GTG Psp1406I 229 AACGTT AA^CGTT RcaI 3220 TCATGA T^CATGA SacII 72 CCGCGG CCGC^GG SphI 1874 GCATGC GCATG^C TaqI 1061 TCGA T^CGA TthHB8I 1061 TCGA T^CGA XcmI 1179 CCATCATCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 33 enzymes) # ################################### AccI 991 GTCTAC GT^[AC][GT]AC 1714 GTCTAC AflII 2671 CTTAAG C^TTAAG 4101 CTTAAG AseI 794 ATTAAT AT^TAAT 917 ATTAAT AsnI 794 ATTAAT AT^TAAT 917 ATTAAT AvaII 881 GGTCC G^G[AT]CC 2119 GGTCC BanII 85 GAGCCC G[GA]GC[CT]^C 1300 GAGCCC BbeI 59 GGCGCC GGCGC^C 119 GGCGCC BfrI 2671 CTTAAG C^TTAAG 4101 CTTAAG BglI 68 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 125 GCCGGCTCGGC BglII 2160 AGATCT A^GATCT 2531 AGATCT BsaBI 1084 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1883 GATAGAAATC BsaWI 323 ACCGGT [AT]^CCGG[AT] 1891 TCCGGA BstAPI 1803 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 1953 GCAAAGGCTGC EaeI 180 CGGCCG [CT]^GGCC[GA] 1191 TGGCCA EcoT14I 282 CCATGG C^C[AT][AT]GG 3633 CCTTGG KasI 59 GGCGCC G^GCGCC 119 GGCGCC MaeII 230 ACGT A^CGT 2255 ACGT MamI 1084 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC 1883 GATAGAAATC MfeI 618 CAATTG C^AATTG 1822 CAATTG MspA1I 72 CCGCGG C[AC]G^C[GT]G 1466 CAGCGG MunI 618 CAATTG C^AATTG 1822 CAATTG NaeI 125 GCCGGC GCC^GGC 178 GCCGGC NarI 59 GGCGCC GG^CGCC 119 GGCGCC NgoMIV 125 GCCGGC G^CCGGC 178 GCCGGC PflMI 1688 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2801 CCATTCTTTGG PshBI 794 ATTAAT AT^TAAT 917 ATTAAT SexAI 1528 ACCAGGT A^CC[AT]GGT 3370 ACCTGGT SfoI 59 GGCGCC GGC^GCC 119 GGCGCC SmaI 40 CCCGGG CCC^GGG 88 CCCGGG StyI 282 CCATGG C^C[AT][AT]GG 3633 CCTTGG Van91I 1688 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2801 CCATTCTTTGG XmaCI 40 CCCGGG C^CCGGG 88 CCCGGG XmaI 40 CCCGGG C^CCGGG 88 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 94 enzymes) # ######################################### AccII AciI AcsI AcyI AfaI AluI AlwI ApoI AspHI AvaI BanI BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaJI BsgI BsiHKAI BsiYI BslI BsmAI BsoBI Bsp1286I Bsp1407I BsrFI BsrGI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraI DsaI EcoRII Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeI MaeIII MboI MboII MflI MnlI MseI MslI MspI MvaI MvnI MwoI NciI NdeI NdeII NlaIII NlaIV PleI PstI RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI SspI TfiI Tru9I TseI Tsp45I Tsp509I TspRI XbaI XhoII