##################################
# NO CUT ENZYMES ( 70 enzymes) #
##################################
AatII AhdI Aor51HI ApaI AscI
Asp700I AspEI AspI AviII AvrII
BamHI BbvCI BlnI BsaI BseRI
BsiWI BspDI BspLU11I BssHII Bst1107I
BstBI BstEII BstPI BstZ17I ClaI
CpoI DraII DraIII Eam1105I EarI
Eco47III EcoNI EcoO109I EcoO65I FauI
FseI FspI HindIII HpaI Ksp632I
MluI NheI NotI NruI NspV
PacI PaeR7I PflFI PmeI PpuMI
PshAI PvuI PvuII RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SnaBI SpeI Sse8387I
StuI SwaI Tth111I XhoI XmnI
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
Acc65I 3622 GGTACC G^GTACC
AccIII 1891 TCCGGA T^CCGGA
AclI 229 AACGTT AA^CGTT
AflIII 2255 ACGTGT A^C[GA][CT]GT
AgeI 323 ACCGGT A^CCGGT
Alw44I 4043 GTGCAC G^TGCAC
AlwNI 1530 CAGGTACTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 4337 CCTAAGG CC^T[ACGT]AGG
ApaLI 4043 GTGCAC G^TGCAC
Asp718I 3622 GGTACC G^GTACC
BalI 1191 TGGCCA TGG^CCA
BbrPI 2254 CACGTG CAC^GTG
BcgI 2037 GCAAATGCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 3777 GTATCC GTATCC(6/5)
BclI 888 TGATCA T^GATCA
BlpI 1278 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1278 GCTCAGC GC^T[ACGT]AGC
BsaAI 2254 CACGTG [CT]AC^GT[GA]
BseAI 1891 TCCGGA T^CCGGA
BsiEI 180 CGGCCG CG[GA][CT]^CG
BsmBI 3268 GAGACG CGTCTC(1/5)
BsmFI 2465 GGGAC GGGAC(10/14)
BsmI 2944 GAATGC GAATGC(1/-1)
BspEI 1891 TCCGGA T^CCGGA
BspHI 3220 TCATGA T^CATGA
BspMI 1521 ACCTGC ACCTGC(4/8)
BsrBI 2321 GAGCGG CCGCTC(-3/-3)
BsrDI 4096 CATTGC GCAATG(2/0)
BssSI 1844 CACGAG CACGAG(-5/-1)
BstXI 1140 CCATCACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 4337 CCTAAGG CC^T[ACGT]AGG
CelII 1278 GCTCAGC GC^T[ACGT]AGC
DrdI 2143 GACCAAGCTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 180 CGGCCG C^GGCCG
EclXI 180 CGGCCG C^GGCCG
Eco52I 180 CGGCCG C^GGCCG
Eco81I 4337 CCTAAGG CC^T[ACGT]AGG
EcoRI 1796 GAATTC G^AATTC
EcoRV 2730 GATATC GAT^ATC
EcoT22I 2041 ATGCAT ATGCA^T
FbaI 888 TGATCA T^GATCA
HincII 308 GTCAAC GT[CT]^[GA]AC
HindII 308 GTCAAC GT[CT]^[GA]AC
KpnI 3622 GGTACC GGTAC^C
KspI 72 CCGCGG CCGC^GG
MluNI 1191 TGGCCA TGG^CCA
MroI 1891 TCCGGA T^CCGGA
MscI 1191 TGGCCA TGG^CCA
NcoI 282 CCATGG C^CATGG
NsiI 2041 ATGCAT ATGCA^T
NspI 1874 GCATGC [GA]CATG^[CT]
PinAI 323 ACCGGT A^CCGGT
PmaCI 2254 CACGTG CAC^GTG
PmlI 2254 CACGTG CAC^GTG
Psp1406I 229 AACGTT AA^CGTT
RcaI 3220 TCATGA T^CATGA
SacII 72 CCGCGG CCGC^GG
SphI 1874 GCATGC GCATG^C
TaqI 1061 TCGA T^CGA
TthHB8I 1061 TCGA T^CGA
XcmI 1179 CCATCATCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 33 enzymes) #
###################################
AccI 991 GTCTAC GT^[AC][GT]AC
1714 GTCTAC
AflII 2671 CTTAAG C^TTAAG
4101 CTTAAG
AseI 794 ATTAAT AT^TAAT
917 ATTAAT
AsnI 794 ATTAAT AT^TAAT
917 ATTAAT
AvaII 881 GGTCC G^G[AT]CC
2119 GGTCC
BanII 85 GAGCCC G[GA]GC[CT]^C
1300 GAGCCC
BbeI 59 GGCGCC GGCGC^C
119 GGCGCC
BfrI 2671 CTTAAG C^TTAAG
4101 CTTAAG
BglI 68 GCCGCCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
125 GCCGGCTCGGC
BglII 2160 AGATCT A^GATCT
2531 AGATCT
BsaBI 1084 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1883 GATAGAAATC
BsaWI 323 ACCGGT [AT]^CCGG[AT]
1891 TCCGGA
BstAPI 1803 GCAACAACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1953 GCAAAGGCTGC
EaeI 180 CGGCCG [CT]^GGCC[GA]
1191 TGGCCA
EcoT14I 282 CCATGG C^C[AT][AT]GG
3633 CCTTGG
KasI 59 GGCGCC G^GCGCC
119 GGCGCC
MaeII 230 ACGT A^CGT
2255 ACGT
MamI 1084 GATAGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1883 GATAGAAATC
MfeI 618 CAATTG C^AATTG
1822 CAATTG
MspA1I 72 CCGCGG C[AC]G^C[GT]G
1466 CAGCGG
MunI 618 CAATTG C^AATTG
1822 CAATTG
NaeI 125 GCCGGC GCC^GGC
178 GCCGGC
NarI 59 GGCGCC GG^CGCC
119 GGCGCC
NgoMIV 125 GCCGGC G^CCGGC
178 GCCGGC
PflMI 1688 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2801 CCATTCTTTGG
PshBI 794 ATTAAT AT^TAAT
917 ATTAAT
SexAI 1528 ACCAGGT A^CC[AT]GGT
3370 ACCTGGT
SfoI 59 GGCGCC GGC^GCC
119 GGCGCC
SmaI 40 CCCGGG CCC^GGG
88 CCCGGG
StyI 282 CCATGG C^C[AT][AT]GG
3633 CCTTGG
Van91I 1688 CCATTCAATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2801 CCATTCTTTGG
XmaCI 40 CCCGGG C^CCGGG
88 CCCGGG
XmaI 40 CCCGGG C^CCGGG
88 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 94 enzymes) #
#########################################
AccII AciI AcsI AcyI AfaI
AluI AlwI ApoI AspHI AvaI
BanI BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaHI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsoBI Bsp1286I Bsp1407I BsrFI BsrGI
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraI DsaI EcoRII
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MvnI MwoI
NciI NdeI NdeII NlaIII NlaIV
PleI PstI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI SspBI
SspI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XbaI XhoII