##################################
# NO CUT ENZYMES ( 92 enzymes) #
##################################
AccI AccIII AflII AflIII AgeI
AscI AseI AsnI AspI AviII
AvrII BbeI BbrPI BcgI BclI
BfrI BglII BlnI BseAI BsiWI
BsmBI BspDI BspEI BspLU11I BsrDI
BssHII Bst1107I BstBI BstXI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI EclXI Eco52I EcoRV EcoT22I
FbaI FseI FspI HgaI HincII
HindII HindIII HpaI KasI KspI
MfeI MluI MroI MunI NaeI
NarI NdeI NgoMIV NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SapI ScaI SexAI SfoI SfuI
SgrAI SmaI SnaBI SpeI SspI
SwaI Tth111I Van91I XbaI XhoI
XmaCI XmaI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AatII 2726 GACGTC GACGT^C
Acc65I 1308 GGTACC G^GTACC
AclI 1730 AACGTT AA^CGTT
AcyI 2726 GACGTC G[GA]^CG[CT]C
AhdI 1224 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2598 GTGCAC G^TGCAC
Aor51HI 715 AGCGCT AGC^GCT
ApaLI 2598 GTGCAC G^TGCAC
Asp700I 1034 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1308 GGTACC G^GTACC
AspEI 1224 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1405 GGATCC G^GATCC
BbvCI 1481 GCTGAGG CCTCAGC(-5/-2)
BsaAI 639 TACGTG [CT]AC^GT[GA]
BsaHI 2726 GACGTC G[GA]^CG[CT]C
BsaI 396 GGTCTC GGTCTC(1/5)
BsaWI 619 TCCGGT [AT]^CCGG[AT]
BsgI 1011 GTGCAG GTGCAG(16/14)
BsiEI 863 CGACCG CG[GA][CT]^CG
BspHI 2722 TCATGA T^CATGA
BsrBI 1874 GAGCGG CCGCTC(-3/-3)
BssSI 2371 CTCGTG CACGAG(-5/-1)
BstEII 834 GGTGACC G^GT[ACGT]ACC
BstPI 834 GGTGACC G^GT[ACGT]ACC
Eam1105I 1224 GACCTGTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 715 AGCGCT AGC^GCT
EcoO65I 834 GGTGACC G^GT[ACGT]ACC
EcoRI 2566 GAATTC G^AATTC
Hin1I 2726 GACGTC G[GA]^CG[CT]C
KpnI 1308 GGTACC GGTAC^C
NheI 1837 GCTAGC G^CTAGC
NspI 1089 GCATGC [GA]CATG^[CT]
Psp1406I 1730 AACGTT AA^CGTT
PstI 459 CTGCAG CTGCA^G
RcaI 2722 TCATGA T^CATGA
SbfI 458 CCTGCAGG CCTGCA^GG
SfiI 1187 GGCCATCAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmlI 882 CTCAAG C^T[CT][GA]AG
SphI 1089 GCATGC GCATG^C
Sse8387I 458 CCTGCAGG CCTGCA^GG
XmnI 1034 GAAGCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 36 enzymes) #
###################################
AccII 735 CGCG CG^CG
1170 CGCG
AocI 512 CCTCAGG CC^T[ACGT]AGG
819 CCTGAGG
ApaI 1416 GGGCCC GGGCC^C
2104 GGGCCC
BalI 2067 TGGCCA TGG^CCA
2341 TGGCCA
BanI 417 GGCACC G^G[CT][GA]CC
1308 GGTACC
BciVI 1830 GGATAC GTATCC(6/5)
1521 GTATCC
BglI 1188 GCCATCAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1739 GCCCAGCAGGC
BpmI 1962 CTCCAG CTGGAG(16/14)
1047 CTGGAG
BsaBI 275 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1400 GATGTGGATC
BsmI 2093 GAATGC GAATGC(1/-1)
2187 GCATTC
Bsp1407I 382 TGTACA T^GTACA
1054 TGTACA
BsrFI 672 ACCGGC [GA]^CCGG[CT]
2057 ACCGGC
BsrGI 382 TGTACA T^GTACA
1054 TGTACA
BstAPI 338 GCACAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
452 GCAGGTCCTGC
BstUI 735 CGCG CG^CG
1170 CGCG
BstYI 101 AGATCC [GA]^GATC[CT]
1405 GGATCC
Bsu36I 512 CCTCAGG CC^T[ACGT]AGG
819 CCTGAGG
Cfr10I 672 ACCGGC [GA]^CCGG[CT]
2057 ACCGGC
DsaI 1551 CCATGG C^C[GA][CT]GG
1617 CCATGG
EaeI 2067 TGGCCA [CT]^GGCC[GA]
2341 TGGCCA
Eco81I 512 CCTCAGG CC^T[ACGT]AGG
819 CCTGAGG
HaeII 715 AGCGCT [GA]GCGC^[CT]
916 GGCGCT
MamI 275 GATGTGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
1400 GATGTGGATC
MflI 101 AGATCC [GA]^GATC[CT]
1405 GGATCC
MluNI 2067 TGGCCA TGG^CCA
2341 TGGCCA
MscI 2067 TGGCCA TGG^CCA
2341 TGGCCA
MseI 856 TTAA T^TAA
2554 TTAA
MslI 2587 CATGTGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2660 CACCAAGATG
MvnI 735 CGCG CG^CG
1170 CGCG
NcoI 1551 CCATGG C^CATGG
1617 CCATGG
PshAI 936 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2615 GACTTTGGTC
SspBI 382 TGTACA T^GTACA
1054 TGTACA
StuI 463 AGGCCT AGG^CCT
577 AGGCCT
Tru9I 856 TTAA T^TAA
2554 TTAA
XcmI 61 CCACCTTTCCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1551 CCATGGATTCCTTGG
XhoII 101 AGATCC [GA]^GATC[CT]
1405 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 89 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
AlwNI ApoI AspHI AvaI AvaII
BanII BbsI BbvI BcnI BfaI
BlpI BmyI Bpu1102I BpuAI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI Cac8I CelII
CfoI Cfr13I DdeI DpnII DraII
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PpuMI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I