##################################
# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI ApaI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BbeI BcgI
BfrI BglI BlnI BsaBI BsaHI
BsaWI BseAI BsiWI BsmBI BspDI
BspEI BspHI BspLU11I BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstZ17I ClaI CpoI DraI DraIII
DrdI Eam1105I EcoO65I EcoRV EcoT22I
FseI FspI Hin1I HpaI KasI
KpnI KspI MamI MluI MroI
NaeI NarI NgoMIV NotI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SspI SwaI Tth111I
Van91I XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 28 enzymes) #
###################################
Alw44I 207 GTGCAC G^TGCAC
AocI 1369 CCTGAGG CC^T[ACGT]AGG
ApaLI 207 GTGCAC G^TGCAC
BbrPI 1155 CACGTG CAC^GTG
BclI 2583 TGATCA T^GATCA
BcnI 971 CCCGG CC^[GC]GG
BsiEI 262 CGGCCG CG[GA][CT]^CG
BsrFI 1130 ACCGGC [GA]^CCGG[CT]
BstXI 556 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 1369 CCTGAGG CC^T[ACGT]AGG
Cfr10I 1130 ACCGGC [GA]^CCGG[CT]
EagI 262 CGGCCG C^GGCCG
EclXI 262 CGGCCG C^GGCCG
Eco52I 262 CGGCCG C^GGCCG
Eco81I 1369 CCTGAGG CC^T[ACGT]AGG
FbaI 2583 TGATCA T^GATCA
HindIII 848 AAGCTT A^AGCTT
MfeI 613 CAATTG C^AATTG
MslI 153 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 613 CAATTG C^AATTG
NciI 971 CCCGG CC^[GC]GG
NdeI 3574 CATATG CA^TATG
PaeR7I 916 CTCGAG C^TCGAG
PmaCI 1155 CACGTG CAC^GTG
PmlI 1155 CACGTG CAC^GTG
SapI 1927 GCTCTTC GCTCTTC(1/4)
ScaI 870 AGTACT AGT^ACT
XhoI 916 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 63 GTCTAC GT^[AC][GT]AC
2352 GTCTAC
AccII 242 CGCG CG^CG
266 CGCG
AcsI 2422 GAATTC [GA]^AATT[CT]
2680 GAATTC
AflIII 2495 ACGTGT A^C[GA][CT]GT
2820 ACGTGT
Aor51HI 94 AGCGCT AGC^GCT
467 AGCGCT
ApoI 2422 GAATTC [GA]^AATT[CT]
2680 GAATTC
AvaI 583 CCCGAG C^[CT]CG[GA]G
916 CTCGAG
BalI 1151 TGGCCA TGG^CCA
2877 TGGCCA
BciVI 2491 GGATAC GTATCC(6/5)
2155 GTATCC
BlpI 1097 GCTCAGC GC^T[ACGT]AGC
3243 GCTCAGC
Bpu1102I 1097 GCTCAGC GC^T[ACGT]AGC
3243 GCTCAGC
BsmI 2610 GAATGC GAATGC(1/-1)
2953 GAATGC
BsoBI 583 CCCGAG C^[CT]CG[GA]G
916 CTCGAG
BstAPI 209 GCACCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2667 GCAGGAGGTGC
BstUI 242 CGCG CG^CG
266 CGCG
CelII 1097 GCTCAGC GC^T[ACGT]AGC
3243 GCTCAGC
Eco47III 94 AGCGCT AGC^GCT
467 AGCGCT
EcoNI 1893 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2482 CCTAACGTAGG
EcoRI 2422 GAATTC G^AATTC
2680 GAATTC
HapII 972 CCGG C^CGG
1131 CCGG
HgaI 129 GACGC GACGC(5/10)
2049 GACGC
HpaII 972 CCGG C^CGG
1131 CCGG
MluNI 1151 TGGCCA TGG^CCA
2877 TGGCCA
MscI 1151 TGGCCA TGG^CCA
2877 TGGCCA
MspI 972 CCGG C^CGG
1131 CCGG
MvnI 242 CGCG CG^CG
266 CGCG
NcoI 658 CCATGG C^CATGG
1237 CCATGG
SbfI 530 CCTGCAGG CCTGCA^GG
1983 CCTGCAGG
SphI 864 GCATGC GCATG^C
1102 GCATGC
Sse8387I 530 CCTGCAGG CCTGCA^GG
1983 CCTGCAGG
XbaI 3174 TCTAGA T^CTAGA
3189 TCTAGA
XcmI 1047 CCATGTTGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1967 CCATTCTTCAACTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaII BamHI BanI BanII
BbsI BbvCI BbvI BfaI BglII
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I Bsp1407I
BspMI BsrDI BsrGI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EarI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HhaI HinP1I HincII HindII HinfI
HphI ItaI Ksp632I MaeI MaeII
MaeIII MboI MboII MflI MnlI
MseI MspA1I MvaI MwoI NdeII
NheI NlaIII NlaIV NspI PleI
PpuMI PshAI PstI PvuII RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI SspBI StuI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII