################################## # NO CUT ENZYMES ( 94 enzymes) # ################################## AatII Acc65I AccIII AclI AcyI AflII AgeI AhdI ApaI AscI AseI AsnI Asp700I Asp718I AspEI AspI AviII AvrII BbeI BcgI BfrI BglI BlnI BsaBI BsaHI BsaWI BseAI BsiWI BsmBI BspDI BspEI BspHI BspLU11I BsrBI BssHII BssSI Bst1107I BstBI BstEII BstPI BstZ17I ClaI CpoI DraI DraIII DrdI Eam1105I EcoO65I EcoRV EcoT22I FseI FspI Hin1I HpaI KasI KpnI KspI MamI MluI MroI NaeI NarI NgoMIV NotI NruI NsiI NspV PacI PflFI PflMI PinAI PmeI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SexAI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI SspI SwaI Tth111I Van91I XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 28 enzymes) # ################################### Alw44I 207 GTGCAC G^TGCAC AocI 1369 CCTGAGG CC^T[ACGT]AGG ApaLI 207 GTGCAC G^TGCAC BbrPI 1155 CACGTG CAC^GTG BclI 2583 TGATCA T^GATCA BcnI 971 CCCGG CC^[GC]GG BsiEI 262 CGGCCG CG[GA][CT]^CG BsrFI 1130 ACCGGC [GA]^CCGG[CT] BstXI 556 CCAAAAGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 1369 CCTGAGG CC^T[ACGT]AGG Cfr10I 1130 ACCGGC [GA]^CCGG[CT] EagI 262 CGGCCG C^GGCCG EclXI 262 CGGCCG C^GGCCG Eco52I 262 CGGCCG C^GGCCG Eco81I 1369 CCTGAGG CC^T[ACGT]AGG FbaI 2583 TGATCA T^GATCA HindIII 848 AAGCTT A^AGCTT MfeI 613 CAATTG C^AATTG MslI 153 CACAGCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MunI 613 CAATTG C^AATTG NciI 971 CCCGG CC^[GC]GG NdeI 3574 CATATG CA^TATG PaeR7I 916 CTCGAG C^TCGAG PmaCI 1155 CACGTG CAC^GTG PmlI 1155 CACGTG CAC^GTG SapI 1927 GCTCTTC GCTCTTC(1/4) ScaI 870 AGTACT AGT^ACT XhoI 916 CTCGAG C^TCGAG ################################### # TWO CUT ENZYMES ( 32 enzymes) # ################################### AccI 63 GTCTAC GT^[AC][GT]AC 2352 GTCTAC AccII 242 CGCG CG^CG 266 CGCG AcsI 2422 GAATTC [GA]^AATT[CT] 2680 GAATTC AflIII 2495 ACGTGT A^C[GA][CT]GT 2820 ACGTGT Aor51HI 94 AGCGCT AGC^GCT 467 AGCGCT ApoI 2422 GAATTC [GA]^AATT[CT] 2680 GAATTC AvaI 583 CCCGAG C^[CT]CG[GA]G 916 CTCGAG BalI 1151 TGGCCA TGG^CCA 2877 TGGCCA BciVI 2491 GGATAC GTATCC(6/5) 2155 GTATCC BlpI 1097 GCTCAGC GC^T[ACGT]AGC 3243 GCTCAGC Bpu1102I 1097 GCTCAGC GC^T[ACGT]AGC 3243 GCTCAGC BsmI 2610 GAATGC GAATGC(1/-1) 2953 GAATGC BsoBI 583 CCCGAG C^[CT]CG[GA]G 916 CTCGAG BstAPI 209 GCACCTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2667 GCAGGAGGTGC BstUI 242 CGCG CG^CG 266 CGCG CelII 1097 GCTCAGC GC^T[ACGT]AGC 3243 GCTCAGC Eco47III 94 AGCGCT AGC^GCT 467 AGCGCT EcoNI 1893 CCTGCAGAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2482 CCTAACGTAGG EcoRI 2422 GAATTC G^AATTC 2680 GAATTC HapII 972 CCGG C^CGG 1131 CCGG HgaI 129 GACGC GACGC(5/10) 2049 GACGC HpaII 972 CCGG C^CGG 1131 CCGG MluNI 1151 TGGCCA TGG^CCA 2877 TGGCCA MscI 1151 TGGCCA TGG^CCA 2877 TGGCCA MspI 972 CCGG C^CGG 1131 CCGG MvnI 242 CGCG CG^CG 266 CGCG NcoI 658 CCATGG C^CATGG 1237 CCATGG SbfI 530 CCTGCAGG CCTGCA^GG 1983 CCTGCAGG SphI 864 GCATGC GCATG^C 1102 GCATGC Sse8387I 530 CCTGCAGG CCTGCA^GG 1983 CCTGCAGG XbaI 3174 TCTAGA T^CTAGA 3189 TCTAGA XcmI 1047 CCATGTTGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 1967 CCATTCTTCAACTGG ######################################### # 3 OR MORE CUT ENZYMES ( 104 enzymes) # ######################################### AciI AfaI AluI AlwI AlwNI AspHI AvaII BamHI BanI BanII BbsI BbvCI BbvI BfaI BglII BmyI BpmI BpuAI BsaAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I Bsp1407I BspMI BsrDI BsrGI BsrI BssKI BstF5I BstNI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HhaI HinP1I HincII HindII HinfI HphI ItaI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MspA1I MvaI MwoI NdeII NheI NlaIII NlaIV NspI PleI PpuMI PshAI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI SmlI SspBI StuI StyI TaqI TfiI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII