################################## # NO CUT ENZYMES ( 96 enzymes) # ################################## AccI AccIII AclI AcsI AflII AflIII AgeI AhdI ApoI AscI AseI AsnI AspEI AspI BamHI BbrPI BcgI BciVI BclI BfrI BlpI Bpu1102I BsaAI BsaBI BseAI BsiWI BsmBI BsmI Bsp1407I BspDI BspEI BspHI BspLU11I BsrDI BsrGI Bst1107I BstBI BstZ17I CelII ClaI CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI HincII HindII HindIII HpaI MamI MfeI MluI MroI MseI MslI MunI NdeI NruI NsiI NspV PacI PflFI PflMI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RcaI RsrII SacI SalI SapI SbfI ScaI SfiI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspBI SspI StuI SwaI Tru9I Tth111I Van91I XbaI XmaCI XmaI ################################### # ONE CUT ENZYMES ( 31 enzymes) # ################################### AatII 2585 GACGTC GACGT^C Acc65I 2712 GGTACC G^GTACC Alw44I 1051 GTGCAC G^TGCAC ApaLI 1051 GTGCAC G^TGCAC Asp700I 2109 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC Asp718I 2712 GGTACC G^GTACC BbeI 1341 GGCGCC GGCGC^C BbsI 2363 GTCTTC GAAGAC(2/6) BbvCI 3048 GCTGAGG CCTCAGC(-5/-2) BpuAI 2363 GTCTTC GAAGAC(2/6) BsaWI 284 TCCGGT [AT]^CCGG[AT] BsiEI 2545 CGGCCG CG[GA][CT]^CG BstAPI 1695 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 912 GGTAACC G^GT[ACGT]ACC BstPI 912 GGTAACC G^GT[ACGT]ACC BstXI 2199 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DrdI 856 GACGTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 2545 CGGCCG C^GGCCG EclXI 2545 CGGCCG C^GGCCG Eco52I 2545 CGGCCG C^GGCCG EcoO65I 912 GGTAACC G^GT[ACGT]ACC FseI 2332 GGCCGGCC GGCCGG^CC KasI 1341 GGCGCC G^GCGCC KpnI 2712 GGTACC GGTAC^C NarI 1341 GGCGCC GG^CGCC NheI 2787 GCTAGC G^CTAGC NotI 2544 GCGGCCGC GC^GGCCGC PshAI 982 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC SfoI 1341 GGCGCC GGC^GCC Tsp509I 428 AATT ^AATT XmnI 2109 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC ################################### # TWO CUT ENZYMES ( 21 enzymes) # ################################### AcyI 1341 GGCGCC G[GA]^CG[CT]C 2585 GACGTC AocI 259 CCTAAGG CC^T[ACGT]AGG 1944 CCTGAGG AvrII 840 CCTAGG C^CTAGG 1563 CCTAGG BglII 226 AGATCT A^GATCT 462 AGATCT BlnI 840 CCTAGG C^CTAGG 1563 CCTAGG BsaHI 1341 GGCGCC G[GA]^CG[CT]C 2585 GACGTC BsgI 251 CTGCAC GTGCAG(16/14) 2250 CTGCAC BsrBI 1021 GAGCGG CCGCTC(-3/-3) 2530 GAGCGG BssSI 1054 CACGAG CACGAG(-5/-1) 1895 CACGAG Bsu36I 259 CCTAAGG CC^T[ACGT]AGG 1944 CCTGAGG EarI 1162 CTCTTC CTCTTC(1/4) 1939 CTCTTC Eco81I 259 CCTAAGG CC^T[ACGT]AGG 1944 CCTGAGG HgaI 2361 GCGTC GACGC(5/10) 2573 GCGTC Hin1I 1341 GGCGCC G[GA]^CG[CT]C 2585 GACGTC Ksp632I 1162 CTCTTC CTCTTC(1/4) 1939 CTCTTC PaeR7I 776 CTCGAG C^TCGAG 951 CTCGAG PstI 2609 CTGCAG CTGCA^G 2914 CTGCAG SexAI 695 ACCAGGT A^CC[AT]GGT 2156 ACCAGGT SphI 617 GCATGC GCATG^C 1885 GCATGC Tsp45I 267 GTCAC ^GT[GC]AC 1555 GTGAC XhoI 776 CTCGAG C^TCGAG 951 CTCGAG ######################################### # 3 OR MORE CUT ENZYMES ( 110 enzymes) # ######################################### AccII AciI AfaI AluI AlwI AlwNI Aor51HI ApaI AspHI AvaI AvaII AviII BalI BanI BanII BbvI BcnI BfaI BglI BmyI BpmI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrFI BsrI BssHII BssKI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII DraIII DsaI EaeI Eco47III EcoNI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI FspI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI KspI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MspA1I MspI MvaI MvnI MwoI NaeI NciI NcoI NdeII NgoMIV NlaIII NlaIV NspI PleI PpuMI PvuII RsaI SacII Sau3AI Sau96I ScrFI SfaNI SfcI SmlI StyI TaqI TfiI TseI TspRI TthHB8I XcmI XhoII