##################################
# NO CUT ENZYMES ( 96 enzymes) #
##################################
AccI AccIII AclI AcsI AflII
AflIII AgeI AhdI ApoI AscI
AseI AsnI AspEI AspI BamHI
BbrPI BcgI BciVI BclI BfrI
BlpI Bpu1102I BsaAI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrDI BsrGI
Bst1107I BstBI BstZ17I CelII ClaI
CpoI DraI Eam1105I EcoRI EcoRV
EcoT22I FbaI HincII HindII HindIII
HpaI MamI MfeI MluI MroI
MseI MslI MunI NdeI NruI
NsiI NspV PacI PflFI PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SfiI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspBI SspI StuI SwaI
Tru9I Tth111I Van91I XbaI XmaCI
XmaI
###################################
# ONE CUT ENZYMES ( 31 enzymes) #
###################################
AatII 2585 GACGTC GACGT^C
Acc65I 2712 GGTACC G^GTACC
Alw44I 1051 GTGCAC G^TGCAC
ApaLI 1051 GTGCAC G^TGCAC
Asp700I 2109 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 2712 GGTACC G^GTACC
BbeI 1341 GGCGCC GGCGC^C
BbsI 2363 GTCTTC GAAGAC(2/6)
BbvCI 3048 GCTGAGG CCTCAGC(-5/-2)
BpuAI 2363 GTCTTC GAAGAC(2/6)
BsaWI 284 TCCGGT [AT]^CCGG[AT]
BsiEI 2545 CGGCCG CG[GA][CT]^CG
BstAPI 1695 GCAGGTTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 912 GGTAACC G^GT[ACGT]ACC
BstPI 912 GGTAACC G^GT[ACGT]ACC
BstXI 2199 CCAGGGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DrdI 856 GACGTGAGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2545 CGGCCG C^GGCCG
EclXI 2545 CGGCCG C^GGCCG
Eco52I 2545 CGGCCG C^GGCCG
EcoO65I 912 GGTAACC G^GT[ACGT]ACC
FseI 2332 GGCCGGCC GGCCGG^CC
KasI 1341 GGCGCC G^GCGCC
KpnI 2712 GGTACC GGTAC^C
NarI 1341 GGCGCC GG^CGCC
NheI 2787 GCTAGC G^CTAGC
NotI 2544 GCGGCCGC GC^GGCCGC
PshAI 982 GACCTTCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SfoI 1341 GGCGCC GGC^GCC
Tsp509I 428 AATT ^AATT
XmnI 2109 GAATAGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcyI 1341 GGCGCC G[GA]^CG[CT]C
2585 GACGTC
AocI 259 CCTAAGG CC^T[ACGT]AGG
1944 CCTGAGG
AvrII 840 CCTAGG C^CTAGG
1563 CCTAGG
BglII 226 AGATCT A^GATCT
462 AGATCT
BlnI 840 CCTAGG C^CTAGG
1563 CCTAGG
BsaHI 1341 GGCGCC G[GA]^CG[CT]C
2585 GACGTC
BsgI 251 CTGCAC GTGCAG(16/14)
2250 CTGCAC
BsrBI 1021 GAGCGG CCGCTC(-3/-3)
2530 GAGCGG
BssSI 1054 CACGAG CACGAG(-5/-1)
1895 CACGAG
Bsu36I 259 CCTAAGG CC^T[ACGT]AGG
1944 CCTGAGG
EarI 1162 CTCTTC CTCTTC(1/4)
1939 CTCTTC
Eco81I 259 CCTAAGG CC^T[ACGT]AGG
1944 CCTGAGG
HgaI 2361 GCGTC GACGC(5/10)
2573 GCGTC
Hin1I 1341 GGCGCC G[GA]^CG[CT]C
2585 GACGTC
Ksp632I 1162 CTCTTC CTCTTC(1/4)
1939 CTCTTC
PaeR7I 776 CTCGAG C^TCGAG
951 CTCGAG
PstI 2609 CTGCAG CTGCA^G
2914 CTGCAG
SexAI 695 ACCAGGT A^CC[AT]GGT
2156 ACCAGGT
SphI 617 GCATGC GCATG^C
1885 GCATGC
Tsp45I 267 GTCAC ^GT[GC]AC
1555 GTGAC
XhoI 776 CTCGAG C^TCGAG
951 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 110 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AlwNI Aor51HI ApaI AspHI AvaI
AvaII AviII BalI BanI BanII
BbvI BcnI BfaI BglI BmyI
BpmI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI BsoBI
Bsp1286I BspMI BsrFI BsrI BssHII
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DraIII DsaI EaeI
Eco47III EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI FspI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI KspI MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NaeI NciI
NcoI NdeII NgoMIV NlaIII NlaIV
NspI PleI PpuMI PvuII RsaI
SacII Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI TspRI TthHB8I XcmI XhoII