##################################
# NO CUT ENZYMES ( 74 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflIII AgeI Aor51HI ApoI
AscI AseI AsnI AviII BamHI
BbeI BcgI BciVI BglII BsaBI
BsaHI BsaWI BseAI BsiWI BspEI
BspHI BspLU11I BssHII Bst1107I BstAPI
BstBI BstZ17I CpoI DraI Eco47III
EcoRI FseI FspI HaeII Hin1I
HpaI KasI KspI MamI MfeI
MluI MroI MunI NarI NruI
NspV PacI PaeR7I PinAI PmeI
PshBI Psp1406I PvuI RcaI RsrII
SacII SalI SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI Sse8387I
SspI SwaI XbaI XhoI
###################################
# ONE CUT ENZYMES ( 59 enzymes) #
###################################
Acc65I 3041 GGTACC G^GTACC
AccII 2780 CGCG CG^CG
AflII 1999 CTTAAG C^TTAAG
AhdI 962 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 637 GTGCAC G^TGCAC
ApaLI 637 GTGCAC G^TGCAC
Asp700I 1416 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 3041 GGTACC G^GTACC
AspEI 962 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2012 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 3270 CCTAGG C^CTAGG
BclI 2113 TGATCA T^GATCA
BfrI 1999 CTTAAG C^TTAAG
BlnI 3270 CCTAGG C^CTAGG
BlpI 2382 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 2382 GCTCAGC GC^T[ACGT]AGC
BsiEI 2983 CGGCCG CG[GA][CT]^CG
BsmI 1604 GAATGC GAATGC(1/-1)
BspDI 419 ATCGAT AT^CGAT
BsrFI 156 GCCGGC [GA]^CCGG[CT]
BstEII 959 GGTGACC G^GT[ACGT]ACC
BstPI 959 GGTGACC G^GT[ACGT]ACC
BstUI 2780 CGCG CG^CG
BstXI 2353 CCATCTAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2349 AGATCC [GA]^GATC[CT]
CelII 2382 GCTCAGC GC^T[ACGT]AGC
Cfr10I 156 GCCGGC [GA]^CCGG[CT]
ClaI 419 ATCGAT AT^CGAT
DraIII 3118 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2231 GACAAGGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 2983 CGGCCG C^GGCCG
Eam1105I 962 GACCTAATGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2983 CGGCCG C^GGCCG
Eco52I 2983 CGGCCG C^GGCCG
EcoO65I 959 GGTGACC G^GT[ACGT]ACC
EcoT22I 2946 ATGCAT ATGCA^T
FauI 442 GCGGG CCCGC(4/6)
FbaI 2113 TGATCA T^GATCA
HgaI 1914 GACGC GACGC(5/10)
HindIII 293 AAGCTT A^AGCTT
KpnI 3041 GGTACC GGTAC^C
MflI 2349 AGATCC [GA]^GATC[CT]
MvnI 2780 CGCG CG^CG
NaeI 156 GCCGGC GCC^GGC
NcoI 653 CCATGG C^CATGG
NdeI 557 CATATG CA^TATG
NgoMIV 156 GCCGGC G^CCGGC
NheI 3263 GCTAGC G^CTAGC
NotI 2982 GCGGCCGC GC^GGCCGC
NsiI 2946 ATGCAT ATGCA^T
PflFI 2012 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1990 GACAGAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1266 CTGCAG CTGCA^G
ScaI 2919 AGTACT AGT^ACT
SpeI 2333 ACTAGT A^CTAGT
SphI 2948 GCATGC GCATG^C
Tth111I 2012 GACGATGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 2349 AGATCC [GA]^GATC[CT]
XmnI 1416 GAAAGGGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AlwNI 596 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
2567 CAGCAGCTG
ApaI 1447 GGGCCC GGGCC^C
2464 GGGCCC
BalI 2799 TGGCCA TGG^CCA
3053 TGGCCA
BbrPI 23 CACGTG CAC^GTG
473 CACGTG
BglI 663 GCCTGTCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
3129 GCCCAGGTGGC
BsgI 1205 CTGCAC GTGCAG(16/14)
1329 GTGCAG
BsmBI 2102 CGTCTC CGTCTC(1/5)
1912 GAGACG
Bsp1407I 253 TGTACA T^GTACA
381 TGTACA
BsrBI 2499 CCGCTC CCGCTC(-3/-3)
409 GAGCGG
BsrDI 834 CATTGC GCAATG(2/0)
3414 GCAATG
BsrGI 253 TGTACA T^GTACA
381 TGTACA
BssSI 3439 CACGAG CACGAG(-5/-1)
1754 CTCGTG
HincII 2678 GTCAAC GT[CT]^[GA]AC
2961 GTCAAC
HindII 2678 GTCAAC GT[CT]^[GA]AC
2961 GTCAAC
MluNI 2799 TGGCCA TGG^CCA
3053 TGGCCA
MscI 2799 TGGCCA TGG^CCA
3053 TGGCCA
NspI 1038 ACATGC [GA]CATG^[CT]
2948 GCATGC
PmaCI 23 CACGTG CAC^GTG
473 CACGTG
PmlI 23 CACGTG CAC^GTG
473 CACGTG
SapI 1133 GCTCTTC GCTCTTC(1/4)
2761 GCTCTTC
SmaI 1730 CCCGGG CCC^GGG
2685 CCCGGG
SmlI 1999 CTTAAG C^T[CT][GA]AG
2871 CTTGAG
SspBI 253 TGTACA T^GTACA
381 TGTACA
StuI 582 AGGCCT AGG^CCT
661 AGGCCT
XmaCI 1730 CCCGGG C^CCGGG
2685 CCCGGG
XmaI 1730 CCCGGG C^CCGGG
2685 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 99 enzymes) #
#########################################
AciI AfaI AluI AlwI AocI
AspHI AvaI AvaII BanI BanII
BbsI BbvCI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaAI BsaI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI Bsu36I
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoNI EcoO109I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MnlI MseI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV PflMI PleI PpuMI
PvuII RsaI SacI Sau3AI Sau96I
ScrFI SfaNI SfcI StyI TaqI
TfiI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I Van91I XcmI