################################## # NO CUT ENZYMES ( 76 enzymes) # ################################## AccI AflII AgeI AhdI Alw44I AlwNI ApaI ApaLI AscI AseI AsnI Asp700I AspEI AspI AviII AvrII BbeI BclI BfrI BlnI BsiWI BsmI BspLU11I BssHII BssSI Bst1107I BstBI BstZ17I CpoI DraI Eam1105I EcoRI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KasI KspI MfeI MluI MunI NarI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PflFI PinAI PmeI PshAI PshBI PvuI RsrII SacII SalI SapI SexAI SfiI SfoI SfuI SgrAI SmlI SnaBI SpeI SwaI Tth111I XhoI XmnI ################################### # ONE CUT ENZYMES ( 63 enzymes) # ################################### AatII 889 GACGTC GACGT^C Acc65I 906 GGTACC G^GTACC AccII 924 CGCG CG^CG AccIII 2478 TCCGGA T^CCGGA AclI 3108 AACGTT AA^CGTT Aor51HI 1299 AGCGCT AGC^GCT Asp718I 906 GGTACC G^GTACC BamHI 1803 GGATCC G^GATCC BbrPI 1566 CACGTG CAC^GTG BbvCI 1775 GCTGAGG CCTCAGC(-5/-2) BglI 2609 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BlpI 866 GCTAAGC GC^T[ACGT]AGC Bpu1102I 866 GCTAAGC GC^T[ACGT]AGC BsaAI 1566 CACGTG [CT]AC^GT[GA] BsaBI 2167 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BseAI 2478 TCCGGA T^CCGGA BsiEI 560 CGGCCG CG[GA][CT]^CG BsmBI 806 GAGACG CGTCTC(1/5) Bsp1407I 1560 TGTACA T^GTACA BspDI 2174 ATCGAT AT^CGAT BspEI 2478 TCCGGA T^CCGGA BspHI 549 TCATGA T^CATGA BsrBI 1781 GAGCGG CCGCTC(-3/-3) BsrGI 1560 TGTACA T^GTACA BstAPI 1064 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstEII 986 GGTCACC G^GT[ACGT]ACC BstPI 986 GGTCACC G^GT[ACGT]ACC BstUI 924 CGCG CG^CG CelII 866 GCTAAGC GC^T[ACGT]AGC ClaI 2174 ATCGAT AT^CGAT DrdI 2468 GACATCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EagI 560 CGGCCG C^GGCCG EclXI 560 CGGCCG C^GGCCG Eco47III 1299 AGCGCT AGC^GCT Eco52I 560 CGGCCG C^GGCCG EcoNI 3068 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG EcoO65I 986 GGTCACC G^GT[ACGT]ACC HaeII 1299 AGCGCT [GA]GCGC^[CT] HindIII 1537 AAGCTT A^AGCTT KpnI 906 GGTACC GGTAC^C MamI 2167 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC MroI 2478 TCCGGA T^CCGGA MvnI 924 CGCG CG^CG NaeI 2605 GCCGGC GCC^GGC NcoI 2148 CCATGG C^CATGG NgoMIV 2605 GCCGGC G^CCGGC PflMI 1812 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG PmaCI 1566 CACGTG CAC^GTG PmlI 1566 CACGTG CAC^GTG Psp1406I 3108 AACGTT AA^CGTT RcaI 549 TCATGA T^CATGA SbfI 305 CCTGCAGG CCTGCA^GG ScaI 3048 AGTACT AGT^ACT SmaI 434 CCCGGG CCC^GGG SphI 841 GCATGC GCATG^C Sse8387I 305 CCTGCAGG CCTGCA^GG SspBI 1560 TGTACA T^GTACA SspI 347 AATATT AAT^ATT StuI 2378 AGGCCT AGG^CCT Van91I 1812 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG XcmI 33 CCACCTCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG XmaCI 434 CCCGGG C^CCGGG XmaI 434 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 27 enzymes) # ################################### AcsI 172 AAATTT [GA]^AATT[CT] 2417 AAATTT AflIII 1565 ACACGT A^C[GA][CT]GT 1567 ACGTGT AocI 1693 CCTCAGG CC^T[ACGT]AGG 2374 CCTGAGG ApoI 172 AAATTT [GA]^AATT[CT] 2417 AAATTT AvaI 364 CTCGGG C^[CT]CG[GA]G 434 CCCGGG BalI 108 TGGCCA TGG^CCA 1492 TGGCCA BanII 882 GAGCTC G[GA]GC[CT]^C 1109 GAGCTC BciVI 409 GGATAC GTATCC(6/5) 3035 GTATCC BglII 951 AGATCT A^GATCT 2427 AGATCT BsaWI 1251 ACCGGA [AT]^CCGG[AT] 2478 TCCGGA BsoBI 364 CTCGGG C^[CT]CG[GA]G 434 CCCGGG BsrDI 1639 CATTGC GCAATG(2/0) 1632 GCAATG Bsu36I 1693 CCTCAGG CC^T[ACGT]AGG 2374 CCTGAGG CfoI 901 GCGC GCG^C 1300 GCGC DraIII 1285 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG 1515 CACGCCGTG EarI 3012 CTCTTC CTCTTC(1/4) 598 GAAGAG Eco81I 1693 CCTCAGG CC^T[ACGT]AGG 2374 CCTGAGG HhaI 901 GCGC GCG^C 1300 GCGC HinP1I 901 GCGC G^CGC 1300 GCGC Ksp632I 3012 CTCTTC CTCTTC(1/4) 598 GAAGAG MluNI 108 TGGCCA TGG^CCA 1492 TGGCCA MscI 108 TGGCCA TGG^CCA 1492 TGGCCA MseI 1002 TTAA T^TAA 3112 TTAA SacI 882 GAGCTC GAGCT^C 1109 GAGCTC TfiI 931 GATTC G^A[AT]TC 2299 GATTC Tru9I 1002 TTAA T^TAA 3112 TTAA XbaI 637 TCTAGA T^CTAGA 1192 TCTAGA ######################################### # 3 OR MORE CUT ENZYMES ( 92 enzymes) # ######################################### AciI AcyI AfaI AluI AlwI AspHI AvaII BanI BbsI BbvI BcgI BcnI BfaI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI Bsp1286I BspMI BsrFI BsrI BssKI BstF5I BstNI BstXI BstYI Cac8I Cfr10I Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI Hin1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MnlI MslI MspA1I MspI MvaI MwoI NciI NdeII NlaIII NlaIV NspI PleI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StyI TaqI TseI Tsp45I Tsp509I TspRI TthHB8I XhoII