##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
AccI AflII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp700I AspEI AspI AviII
AvrII BbeI BclI BfrI BlnI
BsiWI BsmI BspLU11I BssHII BssSI
Bst1107I BstBI BstZ17I CpoI DraI
Eam1105I EcoRI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KasI KspI MfeI MluI MunI
NarI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PmeI PshAI PshBI PvuI
RsrII SacII SalI SapI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI SwaI Tth111I XhoI
XmnI
###################################
# ONE CUT ENZYMES ( 63 enzymes) #
###################################
AatII 889 GACGTC GACGT^C
Acc65I 906 GGTACC G^GTACC
AccII 924 CGCG CG^CG
AccIII 2478 TCCGGA T^CCGGA
AclI 3108 AACGTT AA^CGTT
Aor51HI 1299 AGCGCT AGC^GCT
Asp718I 906 GGTACC G^GTACC
BamHI 1803 GGATCC G^GATCC
BbrPI 1566 CACGTG CAC^GTG
BbvCI 1775 GCTGAGG CCTCAGC(-5/-2)
BglI 2609 GCCAAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 866 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 866 GCTAAGC GC^T[ACGT]AGC
BsaAI 1566 CACGTG [CT]AC^GT[GA]
BsaBI 2167 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseAI 2478 TCCGGA T^CCGGA
BsiEI 560 CGGCCG CG[GA][CT]^CG
BsmBI 806 GAGACG CGTCTC(1/5)
Bsp1407I 1560 TGTACA T^GTACA
BspDI 2174 ATCGAT AT^CGAT
BspEI 2478 TCCGGA T^CCGGA
BspHI 549 TCATGA T^CATGA
BsrBI 1781 GAGCGG CCGCTC(-3/-3)
BsrGI 1560 TGTACA T^GTACA
BstAPI 1064 GCACTGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 986 GGTCACC G^GT[ACGT]ACC
BstPI 986 GGTCACC G^GT[ACGT]ACC
BstUI 924 CGCG CG^CG
CelII 866 GCTAAGC GC^T[ACGT]AGC
ClaI 2174 ATCGAT AT^CGAT
DrdI 2468 GACATCTGGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EagI 560 CGGCCG C^GGCCG
EclXI 560 CGGCCG C^GGCCG
Eco47III 1299 AGCGCT AGC^GCT
Eco52I 560 CGGCCG C^GGCCG
EcoNI 3068 CCTCTGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 986 GGTCACC G^GT[ACGT]ACC
HaeII 1299 AGCGCT [GA]GCGC^[CT]
HindIII 1537 AAGCTT A^AGCTT
KpnI 906 GGTACC GGTAC^C
MamI 2167 GATTGCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MroI 2478 TCCGGA T^CCGGA
MvnI 924 CGCG CG^CG
NaeI 2605 GCCGGC GCC^GGC
NcoI 2148 CCATGG C^CATGG
NgoMIV 2605 GCCGGC G^CCGGC
PflMI 1812 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1566 CACGTG CAC^GTG
PmlI 1566 CACGTG CAC^GTG
Psp1406I 3108 AACGTT AA^CGTT
RcaI 549 TCATGA T^CATGA
SbfI 305 CCTGCAGG CCTGCA^GG
ScaI 3048 AGTACT AGT^ACT
SmaI 434 CCCGGG CCC^GGG
SphI 841 GCATGC GCATG^C
Sse8387I 305 CCTGCAGG CCTGCA^GG
SspBI 1560 TGTACA T^GTACA
SspI 347 AATATT AAT^ATT
StuI 2378 AGGCCT AGG^CCT
Van91I 1812 CCAAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 33 CCACCTCAGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 434 CCCGGG C^CCGGG
XmaI 434 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 172 AAATTT [GA]^AATT[CT]
2417 AAATTT
AflIII 1565 ACACGT A^C[GA][CT]GT
1567 ACGTGT
AocI 1693 CCTCAGG CC^T[ACGT]AGG
2374 CCTGAGG
ApoI 172 AAATTT [GA]^AATT[CT]
2417 AAATTT
AvaI 364 CTCGGG C^[CT]CG[GA]G
434 CCCGGG
BalI 108 TGGCCA TGG^CCA
1492 TGGCCA
BanII 882 GAGCTC G[GA]GC[CT]^C
1109 GAGCTC
BciVI 409 GGATAC GTATCC(6/5)
3035 GTATCC
BglII 951 AGATCT A^GATCT
2427 AGATCT
BsaWI 1251 ACCGGA [AT]^CCGG[AT]
2478 TCCGGA
BsoBI 364 CTCGGG C^[CT]CG[GA]G
434 CCCGGG
BsrDI 1639 CATTGC GCAATG(2/0)
1632 GCAATG
Bsu36I 1693 CCTCAGG CC^T[ACGT]AGG
2374 CCTGAGG
CfoI 901 GCGC GCG^C
1300 GCGC
DraIII 1285 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
1515 CACGCCGTG
EarI 3012 CTCTTC CTCTTC(1/4)
598 GAAGAG
Eco81I 1693 CCTCAGG CC^T[ACGT]AGG
2374 CCTGAGG
HhaI 901 GCGC GCG^C
1300 GCGC
HinP1I 901 GCGC G^CGC
1300 GCGC
Ksp632I 3012 CTCTTC CTCTTC(1/4)
598 GAAGAG
MluNI 108 TGGCCA TGG^CCA
1492 TGGCCA
MscI 108 TGGCCA TGG^CCA
1492 TGGCCA
MseI 1002 TTAA T^TAA
3112 TTAA
SacI 882 GAGCTC GAGCT^C
1109 GAGCTC
TfiI 931 GATTC G^A[AT]TC
2299 GATTC
Tru9I 1002 TTAA T^TAA
3112 TTAA
XbaI 637 TCTAGA T^CTAGA
1192 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanI BbsI BbvI
BcgI BcnI BfaI BmyI BpmI
BpuAI BsaHI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrFI BsrI
BssKI BstF5I BstNI BstXI BstYI
Cac8I Cfr10I Cfr13I DdeI DpnII
DraII DsaI EaeI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI Hin1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MslI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PpuMI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII