##################################
# NO CUT ENZYMES ( 67 enzymes) #
##################################
AccI AccIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AvrII
BamHI BbrPI BbvCI BcgI BlnI
BsaAI BseAI BsiWI BspEI BspLU11I
BssHII Bst1107I BstEII BstPI BstZ17I
CpoI DraIII DrdI Eam1105I Eco47III
EcoO65I EcoRI EcoRV EcoT22I FseI
HpaI KspI MfeI MluI MroI
MunI NdeI NotI NsiI PacI
PaeR7I PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SbfI ScaI SexAI SfiI SgrAI
SnaBI SpeI SphI Sse8387I SwaI
XbaI XhoI
###################################
# ONE CUT ENZYMES ( 60 enzymes) #
###################################
AatII 828 GACGTC GACGT^C
Acc65I 1499 GGTACC G^GTACC
AclI 2630 AACGTT AA^CGTT
AflII 2293 CTTAAG C^TTAAG
AflIII 2171 ACGTGT A^C[GA][CT]GT
Alw44I 1173 GTGCAC G^TGCAC
AocI 772 CCTGAGG CC^T[ACGT]AGG
ApaLI 1173 GTGCAC G^TGCAC
Asp718I 1499 GGTACC G^GTACC
AspI 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1802 TGCGCA TGC^GCA
BbeI 1578 GGCGCC GGCGC^C
BciVI 127 GTATCC GTATCC(6/5)
BclI 1061 TGATCA T^GATCA
BfrI 2293 CTTAAG C^TTAAG
BglII 843 AGATCT A^GATCT
BlpI 1341 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 1341 GCTGAGC GC^T[ACGT]AGC
BsaBI 1515 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 122 CGGCCG CG[GA][CT]^CG
Bsp1407I 641 TGTACA T^GTACA
BspDI 2023 ATCGAT AT^CGAT
BsrBI 99 CCGCTC CCGCTC(-3/-3)
BsrFI 120 GCCGGC [GA]^CCGG[CT]
BsrGI 641 TGTACA T^GTACA
BstBI 2266 TTCGAA TT^CGAA
BstXI 2600 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 772 CCTGAGG CC^T[ACGT]AGG
CelII 1341 GCTGAGC GC^T[ACGT]AGC
Cfr10I 120 GCCGGC [GA]^CCGG[CT]
ClaI 2023 ATCGAT AT^CGAT
DraI 247 TTTAAA TTT^AAA
EagI 122 CGGCCG C^GGCCG
EclXI 122 CGGCCG C^GGCCG
Eco52I 122 CGGCCG C^GGCCG
Eco81I 772 CCTGAGG CC^T[ACGT]AGG
EcoNI 975 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 1061 TGATCA T^GATCA
FspI 1802 TGCGCA TGC^GCA
HaeII 1578 GGCGCC [GA]GCGC^[CT]
KasI 1578 GGCGCC G^GCGCC
KpnI 1499 GGTACC GGTAC^C
MamI 1515 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NaeI 120 GCCGGC GCC^GGC
NarI 1578 GGCGCC GG^CGCC
NgoMIV 120 GCCGGC G^CCGGC
NheI 798 GCTAGC G^CTAGC
NspV 2266 TTCGAA TT^CGAA
PflFI 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Psp1406I 2630 AACGTT AA^CGTT
SacI 366 GAGCTC GAGCT^C
SfoI 1578 GGCGCC GGC^GCC
SfuI 2266 TTCGAA TT^CGAA
SmaI 104 CCCGGG CCC^GGG
SmlI 2293 CTTAAG C^T[CT][GA]AG
SspBI 641 TGTACA T^GTACA
SspI 232 AATATT AAT^ATT
Tth111I 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 104 CCCGGG C^CCGGG
XmaI 104 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 828 GACGTC G[GA]^CG[CT]C
1578 GGCGCC
Asp700I 2044 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2670 GAATATCTTC
BglI 89 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1399 GCCATCCTGGC
BsaHI 828 GACGTC G[GA]^CG[CT]C
1578 GGCGCC
BsaWI 443 TCCGGT [AT]^CCGG[AT]
1307 ACCGGA
BspHI 324 TCATGA T^CATGA
1590 TCATGA
BsrDI 519 CATTGC GCAATG(2/0)
1571 CATTGC
BstAPI 2200 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2658 GCAAGAAGTGC
EarI 50 CTCTTC CTCTTC(1/4)
304 CTCTTC
Hin1I 828 GACGTC G[GA]^CG[CT]C
1578 GGCGCC
HincII 2476 GTCAAC GT[CT]^[GA]AC
2755 GTTGAC
HindII 2476 GTCAAC GT[CT]^[GA]AC
2755 GTTGAC
HindIII 895 AAGCTT A^AGCTT
2566 AAGCTT
Ksp632I 50 CTCTTC CTCTTC(1/4)
304 CTCTTC
NcoI 596 CCATGG C^CATGG
668 CCATGG
NruI 808 TCGCGA TCG^CGA
1527 TCGCGA
NspI 1169 GCATGT [GA]CATG^[CT]
1585 GCATGT
PflMI 711 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3070 CCAGCCCCTGG
PpuMI 1652 GGGACCC [GA]G^G[AT]CC[CT]
2542 GGGACCT
PshAI 786 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2291 GACTTAAGTC
RcaI 324 TCATGA T^CATGA
1590 TCATGA
SapI 49 GCTCTTC GCTCTTC(1/4)
303 GCTCTTC
TfiI 357 GAATC G^A[AT]TC
2763 GAATC
Van91I 711 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3070 CCAGCCCCTGG
XcmI 572 CCAAGATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2526 CCAGTTCAGGTATGG
XmnI 2044 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2670 GAATATCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 105 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApaI ApoI AspHI
AvaI AvaII BalI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmBI BsmFI BsmI BsoBI Bsp1286I
BspMI BsrI BssKI BssSI BstF5I
BstNI BstUI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI MaeI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MseI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PleI PstI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI StyI TaqI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XhoII