################################## # NO CUT ENZYMES ( 67 enzymes) # ################################## AccI AccIII AgeI AhdI Aor51HI AscI AseI AsnI AspEI AvrII BamHI BbrPI BbvCI BcgI BlnI BsaAI BseAI BsiWI BspEI BspLU11I BssHII Bst1107I BstEII BstPI BstZ17I CpoI DraIII DrdI Eam1105I Eco47III EcoO65I EcoRI EcoRV EcoT22I FseI HpaI KspI MfeI MluI MroI MunI NdeI NotI NsiI PacI PaeR7I PinAI PmaCI PmeI PmlI PshBI PvuI RsrII SacII SalI SbfI ScaI SexAI SfiI SgrAI SnaBI SpeI SphI Sse8387I SwaI XbaI XhoI ################################### # ONE CUT ENZYMES ( 60 enzymes) # ################################### AatII 828 GACGTC GACGT^C Acc65I 1499 GGTACC G^GTACC AclI 2630 AACGTT AA^CGTT AflII 2293 CTTAAG C^TTAAG AflIII 2171 ACGTGT A^C[GA][CT]GT Alw44I 1173 GTGCAC G^TGCAC AocI 772 CCTGAGG CC^T[ACGT]AGG ApaLI 1173 GTGCAC G^TGCAC Asp718I 1499 GGTACC G^GTACC AspI 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC AviII 1802 TGCGCA TGC^GCA BbeI 1578 GGCGCC GGCGC^C BciVI 127 GTATCC GTATCC(6/5) BclI 1061 TGATCA T^GATCA BfrI 2293 CTTAAG C^TTAAG BglII 843 AGATCT A^GATCT BlpI 1341 GCTGAGC GC^T[ACGT]AGC Bpu1102I 1341 GCTGAGC GC^T[ACGT]AGC BsaBI 1515 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC BsiEI 122 CGGCCG CG[GA][CT]^CG Bsp1407I 641 TGTACA T^GTACA BspDI 2023 ATCGAT AT^CGAT BsrBI 99 CCGCTC CCGCTC(-3/-3) BsrFI 120 GCCGGC [GA]^CCGG[CT] BsrGI 641 TGTACA T^GTACA BstBI 2266 TTCGAA TT^CGAA BstXI 2600 CCACAGAAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG Bsu36I 772 CCTGAGG CC^T[ACGT]AGG CelII 1341 GCTGAGC GC^T[ACGT]AGC Cfr10I 120 GCCGGC [GA]^CCGG[CT] ClaI 2023 ATCGAT AT^CGAT DraI 247 TTTAAA TTT^AAA EagI 122 CGGCCG C^GGCCG EclXI 122 CGGCCG C^GGCCG Eco52I 122 CGGCCG C^GGCCG Eco81I 772 CCTGAGG CC^T[ACGT]AGG EcoNI 975 CCTGAACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 1061 TGATCA T^GATCA FspI 1802 TGCGCA TGC^GCA HaeII 1578 GGCGCC [GA]GCGC^[CT] KasI 1578 GGCGCC G^GCGCC KpnI 1499 GGTACC GGTAC^C MamI 1515 GATTGTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC NaeI 120 GCCGGC GCC^GGC NarI 1578 GGCGCC GG^CGCC NgoMIV 120 GCCGGC G^CCGGC NheI 798 GCTAGC G^CTAGC NspV 2266 TTCGAA TT^CGAA PflFI 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC Psp1406I 2630 AACGTT AA^CGTT SacI 366 GAGCTC GAGCT^C SfoI 1578 GGCGCC GGC^GCC SfuI 2266 TTCGAA TT^CGAA SmaI 104 CCCGGG CCC^GGG SmlI 2293 CTTAAG C^T[CT][GA]AG SspBI 641 TGTACA T^GTACA SspI 232 AATATT AAT^ATT Tth111I 2887 GACACCGTC GAC[ACGT]^[ACGT][ACGT]GTC XmaCI 104 CCCGGG C^CCGGG XmaI 104 CCCGGG C^CCGGG ################################### # TWO CUT ENZYMES ( 26 enzymes) # ################################### AcyI 828 GACGTC G[GA]^CG[CT]C 1578 GGCGCC Asp700I 2044 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2670 GAATATCTTC BglI 89 GCCGCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1399 GCCATCCTGGC BsaHI 828 GACGTC G[GA]^CG[CT]C 1578 GGCGCC BsaWI 443 TCCGGT [AT]^CCGG[AT] 1307 ACCGGA BspHI 324 TCATGA T^CATGA 1590 TCATGA BsrDI 519 CATTGC GCAATG(2/0) 1571 CATTGC BstAPI 2200 GCACCTCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC 2658 GCAAGAAGTGC EarI 50 CTCTTC CTCTTC(1/4) 304 CTCTTC Hin1I 828 GACGTC G[GA]^CG[CT]C 1578 GGCGCC HincII 2476 GTCAAC GT[CT]^[GA]AC 2755 GTTGAC HindII 2476 GTCAAC GT[CT]^[GA]AC 2755 GTTGAC HindIII 895 AAGCTT A^AGCTT 2566 AAGCTT Ksp632I 50 CTCTTC CTCTTC(1/4) 304 CTCTTC NcoI 596 CCATGG C^CATGG 668 CCATGG NruI 808 TCGCGA TCG^CGA 1527 TCGCGA NspI 1169 GCATGT [GA]CATG^[CT] 1585 GCATGT PflMI 711 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3070 CCAGCCCCTGG PpuMI 1652 GGGACCC [GA]G^G[AT]CC[CT] 2542 GGGACCT PshAI 786 GACCTCTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC 2291 GACTTAAGTC RcaI 324 TCATGA T^CATGA 1590 TCATGA SapI 49 GCTCTTC GCTCTTC(1/4) 303 GCTCTTC TfiI 357 GAATC G^A[AT]TC 2763 GAATC Van91I 711 CCAGCACTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 3070 CCAGCCCCTGG XcmI 572 CCAAGATCCTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2526 CCAGTTCAGGTATGG XmnI 2044 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2670 GAATATCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 105 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApaI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsmI BsoBI Bsp1286I BspMI BsrI BssKI BssSI BstF5I BstNI BstUI BstYI Cac8I CfoI Cfr13I DdeI DpnII DraII DsaI EaeI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeIII HapII HgaI HhaI HinP1I HinfI HpaII HphI ItaI MaeI MaeII MaeIII MboI MboII MflI MluNI MnlI MscI MseI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PleI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I XhoII