##################################
# NO CUT ENZYMES ( 157 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AviII
AvrII BamHI BbeI BbrPI BbsI
BbvCI BcgI BclI BcnI BfaI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI BspDI BspEI BspMI BsrBI
BsrDI BssHII BssSI Bst1107I BstAPI
BstBI BstEII BstPI BstUI BstZ17I
Bsu36I CelII CfoI ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I FbaI FspI
HaeII HgaI HhaI Hin1I HinP1I
HincII HindII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MluI MroI MunI MvnI NarI
NciI NdeI NheI NotI NruI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PstI PvuI RsrII
SacI SacII SalI SapI SbfI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspI StuI SwaI
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 49 enzymes) #
###################################
AcsI 235 GAATTC [GA]^AATT[CT]
AflIII 6 ACATGT A^C[GA][CT]GT
ApoI 235 GAATTC [GA]^AATT[CT]
AseI 589 ATTAAT AT^TAAT
AsnI 589 ATTAAT AT^TAAT
BalI 496 TGGCCA TGG^CCA
BanI 510 GGTGCC G^G[CT][GA]CC
BbvI 229 GCAGC GCAGC(8/12)
BciVI 529 GGATAC GTATCC(6/5)
BseRI 134 GAGGAG GAGGAG(10/8)
BsgI 449 GTGCAG GTGCAG(16/14)
BsmI 605 GCATTC GAATGC(1/-1)
Bsp1407I 102 TGTACA T^GTACA
BspLU11I 6 ACATGT A^CATGT
BsrFI 439 GCCGGC [GA]^CCGG[CT]
BsrGI 102 TGTACA T^GTACA
Cac8I 439 GCCGGC GC[ACGT]^[ACGT]GC
Cfr10I 439 GCCGGC [GA]^CCGG[CT]
Cfr13I 437 GGGCC G^G[ACGT]CC
DsaI 147 CCATGG C^C[GA][CT]GG
EarI 296 GAAGAG CTCTTC(1/4)
EcoRI 235 GAATTC G^AATTC
EcoRV 161 GATATC GAT^ATC
FauI 540 GCGGG CCCGC(4/6)
Fnu4HI 229 GCAGC GC^[ACGT]GC
FseI 438 GGCCGGCC GGCCGG^CC
HapII 440 CCGG C^CGG
HindIII 377 AAGCTT A^AGCTT
HpaII 440 CCGG C^CGG
HphI 486 TCACC GGTGA(8/7)
ItaI 229 GCAGC GC^[ACGT]GC
Ksp632I 296 GAAGAG CTCTTC(1/4)
MaeIII 485 GTCAC ^GT[ACGT]AC
MluNI 496 TGGCCA TGG^CCA
MscI 496 TGGCCA TGG^CCA
MslI 148 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 440 CCGG C^CGG
NaeI 439 GCCGGC GCC^GGC
NcoI 147 CCATGG C^CATGG
NgoMIV 439 GCCGGC G^CCGGC
NspI 6 ACATGT [GA]CATG^[CT]
PleI 544 GAGTC GAGTC(4/5)
PshBI 589 ATTAAT AT^TAAT
Sau96I 437 GGGCC G^G[ACGT]CC
ScaI 393 AGTACT AGT^ACT
SspBI 102 TGTACA T^GTACA
TseI 229 GCAGC G^C[AT]GC
Tsp45I 485 GTCAC ^GT[GC]AC
XcmI 387 CCACAGAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 205 CCGC CCGC(-3/-1)
540 GCGG
AfaI 103 GTAC GT^AC
394 GTAC
BspHI 304 TCATGA T^CATGA
405 TCATGA
BssKI 325 CCTGG ^CC[ACGT]GG
491 CCTGG
BstNI 325 CCTGG CC^[AT]GG
491 CCTGG
BstXI 141 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
147 CCATGGGAATGG
BstYI 49 GGATCT [GA]^GATC[CT]
75 AGATCC
DdeI 15 CTAAG C^T[ACGT]AG
367 CTCAG
EcoRII 325 CCTGG ^CC[AT]GG
491 CCTGG
EcoT14I 55 CCTTGG C^C[AT][AT]GG
147 CCATGG
EcoT22I 585 ATGCAT ATGCA^T
593 ATGCAT
MflI 49 GGATCT [GA]^GATC[CT]
75 AGATCC
MspA1I 230 CAGCTG C[AC]G^C[GT]G
353 CAGCTG
MvaI 325 CCTGG CC^[AT]GG
491 CCTGG
MwoI 14 GCTAAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
566 GCTTTAATTGC
NlaIV 95 GGAGCC GG[ACGT]^[ACGT]CC
510 GGTGCC
NsiI 585 ATGCAT ATGCA^T
593 ATGCAT
PvuII 230 CAGCTG CAG^CTG
353 CAGCTG
RcaI 304 TCATGA T^CATGA
405 TCATGA
RsaI 103 GTAC GT^AC
394 GTAC
ScrFI 325 CCTGG CC^[ACGT]GG
491 CCTGG
SfaNI 171 GATGC GCATC(5/9)
108 GCATC
StyI 55 CCTTGG C^C[AT][AT]GG
147 CCATGG
TaqI 130 TCGA T^CGA
252 TCGA
TfiI 266 GAATC G^A[AT]TC
402 GAATC
Tsp509I 236 AATT ^AATT
571 AATT
TthHB8I 130 TCGA T^CGA
252 TCGA
XhoII 49 GGATCT [GA]^GATC[CT]
75 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 24 enzymes) #
#########################################
AluI AlwI BanII BmyI BsaJI
BsiYI BslI Bsp1286I BsrI BstF5I
DpnII EaeI FokI HaeIII HinfI
MboI MboII MnlI MseI NdeII
NlaIII Sau3AI Tru9I TspRI