################################## # NO CUT ENZYMES ( 157 enzymes) # ################################## AatII Acc65I AccI AccII AccIII AclI AcyI AflII AgeI AhdI Alw44I AlwNI AocI Aor51HI ApaI ApaLI AscI Asp700I Asp718I AspEI AspHI AspI AvaI AvaII AviII AvrII BamHI BbeI BbrPI BbsI BbvCI BcgI BclI BcnI BfaI BfrI BglI BglII BlnI BlpI BpmI Bpu1102I BpuAI BsaAI BsaBI BsaHI BsaI BsaWI BseAI BsiEI BsiHKAI BsiWI BsmAI BsmBI BsmFI BsoBI BspDI BspEI BspMI BsrBI BsrDI BssHII BssSI Bst1107I BstAPI BstBI BstEII BstPI BstUI BstZ17I Bsu36I CelII CfoI ClaI CpoI DraI DraII DraIII DrdI EagI Eam1105I EclXI Eco47III Eco52I Eco81I EcoNI EcoO109I EcoO65I FbaI FspI HaeII HgaI HhaI Hin1I HinP1I HincII HindII HpaI KasI KpnI KspI MaeI MaeII MamI MfeI MluI MroI MunI MvnI NarI NciI NdeI NheI NotI NruI NspV PacI PaeR7I PflFI PflMI PinAI PmaCI PmeI PmlI PpuMI PshAI Psp1406I PstI PvuI RsrII SacI SacII SalI SapI SbfI SexAI SfcI SfiI SfoI SfuI SgrAI SmaI SmlI SnaBI SpeI SphI Sse8387I SspI StuI SwaI Tth111I Van91I XbaI XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 49 enzymes) # ################################### AcsI 235 GAATTC [GA]^AATT[CT] AflIII 6 ACATGT A^C[GA][CT]GT ApoI 235 GAATTC [GA]^AATT[CT] AseI 589 ATTAAT AT^TAAT AsnI 589 ATTAAT AT^TAAT BalI 496 TGGCCA TGG^CCA BanI 510 GGTGCC G^G[CT][GA]CC BbvI 229 GCAGC GCAGC(8/12) BciVI 529 GGATAC GTATCC(6/5) BseRI 134 GAGGAG GAGGAG(10/8) BsgI 449 GTGCAG GTGCAG(16/14) BsmI 605 GCATTC GAATGC(1/-1) Bsp1407I 102 TGTACA T^GTACA BspLU11I 6 ACATGT A^CATGT BsrFI 439 GCCGGC [GA]^CCGG[CT] BsrGI 102 TGTACA T^GTACA Cac8I 439 GCCGGC GC[ACGT]^[ACGT]GC Cfr10I 439 GCCGGC [GA]^CCGG[CT] Cfr13I 437 GGGCC G^G[ACGT]CC DsaI 147 CCATGG C^C[GA][CT]GG EarI 296 GAAGAG CTCTTC(1/4) EcoRI 235 GAATTC G^AATTC EcoRV 161 GATATC GAT^ATC FauI 540 GCGGG CCCGC(4/6) Fnu4HI 229 GCAGC GC^[ACGT]GC FseI 438 GGCCGGCC GGCCGG^CC HapII 440 CCGG C^CGG HindIII 377 AAGCTT A^AGCTT HpaII 440 CCGG C^CGG HphI 486 TCACC GGTGA(8/7) ItaI 229 GCAGC GC^[ACGT]GC Ksp632I 296 GAAGAG CTCTTC(1/4) MaeIII 485 GTCAC ^GT[ACGT]AC MluNI 496 TGGCCA TGG^CCA MscI 496 TGGCCA TGG^CCA MslI 148 CATGGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG MspI 440 CCGG C^CGG NaeI 439 GCCGGC GCC^GGC NcoI 147 CCATGG C^CATGG NgoMIV 439 GCCGGC G^CCGGC NspI 6 ACATGT [GA]CATG^[CT] PleI 544 GAGTC GAGTC(4/5) PshBI 589 ATTAAT AT^TAAT Sau96I 437 GGGCC G^G[ACGT]CC ScaI 393 AGTACT AGT^ACT SspBI 102 TGTACA T^GTACA TseI 229 GCAGC G^C[AT]GC Tsp45I 485 GTCAC ^GT[GC]AC XcmI 387 CCACAGAGTACTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 28 enzymes) # ################################### AciI 205 CCGC CCGC(-3/-1) 540 GCGG AfaI 103 GTAC GT^AC 394 GTAC BspHI 304 TCATGA T^CATGA 405 TCATGA BssKI 325 CCTGG ^CC[ACGT]GG 491 CCTGG BstNI 325 CCTGG CC^[AT]GG 491 CCTGG BstXI 141 CCAGAGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 147 CCATGGGAATGG BstYI 49 GGATCT [GA]^GATC[CT] 75 AGATCC DdeI 15 CTAAG C^T[ACGT]AG 367 CTCAG EcoRII 325 CCTGG ^CC[AT]GG 491 CCTGG EcoT14I 55 CCTTGG C^C[AT][AT]GG 147 CCATGG EcoT22I 585 ATGCAT ATGCA^T 593 ATGCAT MflI 49 GGATCT [GA]^GATC[CT] 75 AGATCC MspA1I 230 CAGCTG C[AC]G^C[GT]G 353 CAGCTG MvaI 325 CCTGG CC^[AT]GG 491 CCTGG MwoI 14 GCTAAGGTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC 566 GCTTTAATTGC NlaIV 95 GGAGCC GG[ACGT]^[ACGT]CC 510 GGTGCC NsiI 585 ATGCAT ATGCA^T 593 ATGCAT PvuII 230 CAGCTG CAG^CTG 353 CAGCTG RcaI 304 TCATGA T^CATGA 405 TCATGA RsaI 103 GTAC GT^AC 394 GTAC ScrFI 325 CCTGG CC^[ACGT]GG 491 CCTGG SfaNI 171 GATGC GCATC(5/9) 108 GCATC StyI 55 CCTTGG C^C[AT][AT]GG 147 CCATGG TaqI 130 TCGA T^CGA 252 TCGA TfiI 266 GAATC G^A[AT]TC 402 GAATC Tsp509I 236 AATT ^AATT 571 AATT TthHB8I 130 TCGA T^CGA 252 TCGA XhoII 49 GGATCT [GA]^GATC[CT] 75 AGATCC ######################################### # 3 OR MORE CUT ENZYMES ( 24 enzymes) # ######################################### AluI AlwI BanII BmyI BsaJI BsiYI BslI Bsp1286I BsrI BstF5I DpnII EaeI FokI HaeIII HinfI MboI MboII MnlI MseI NdeII NlaIII Sau3AI Tru9I TspRI