##################################
# NO CUT ENZYMES ( 69 enzymes) #
##################################
AatII Acc65I AccII AclI AgeI
Alw44I Aor51HI ApaLI AscI Asp718I
AviII BbeI BbrPI BciVI BglII
BsaBI BsiEI BsiWI BsmBI BspDI
BssHII Bst1107I BstUI BstXI BstZ17I
ClaI CpoI DraI EagI EclXI
Eco47III Eco52I EcoRV EcoT22I FseI
FspI HpaI KasI KpnI KspI
MamI MfeI MluI MslI MunI
MvnI NarI NotI NruI NsiI
PacI PflMI PinAI PmaCI PmeI
PmlI PshAI Psp1406I PvuI RsrII
SacII SexAI SfiI SfoI SgrAI
SpeI SphI SwaI Van91I
###################################
# ONE CUT ENZYMES ( 61 enzymes) #
###################################
AcsI 3411 GAATTC [GA]^AATT[CT]
AcyI 2129 GACGCC G[GA]^CG[CT]C
AflII 1827 CTTAAG C^TTAAG
AhdI 1248 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 3411 GAATTC [GA]^AATT[CT]
AseI 3467 ATTAAT AT^TAAT
AsnI 3467 ATTAAT AT^TAAT
Asp700I 2671 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 1248 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2550 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvrII 2085 CCTAGG C^CTAGG
BamHI 3334 GGATCC G^GATCC
BbvCI 2107 GCTGAGG CCTCAGC(-5/-2)
BcgI 2780 GCAATCCCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 1565 TGATCA T^GATCA
BfrI 1827 CTTAAG C^TTAAG
BlnI 2085 CCTAGG C^CTAGG
BlpI 1948 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1948 GCTCAGC GC^T[ACGT]AGC
BsaHI 2129 GACGCC G[GA]^CG[CT]C
BsaI 2846 GAGACC GGTCTC(1/5)
BsgI 2134 CTGCAC GTGCAG(16/14)
BsmI 1125 GAATGC GAATGC(1/-1)
BspLU11I 2516 ACATGT A^CATGT
BspMI 2245 GCAGGT ACCTGC(4/8)
BsrBI 199 GAGCGG CCGCTC(-3/-3)
BssSI 922 CACGAG CACGAG(-5/-1)
BstBI 1363 TTCGAA TT^CGAA
BstEII 1860 GGTCACC G^GT[ACGT]ACC
BstPI 1860 GGTCACC G^GT[ACGT]ACC
CelII 1948 GCTCAGC GC^T[ACGT]AGC
DraIII 3238 CACTTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1632 GACCCTCAAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 1248 GACCTTGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 1860 GGTCACC G^GT[ACGT]ACC
EcoRI 3411 GAATTC G^AATTC
FbaI 1565 TGATCA T^GATCA
HgaI 2129 GACGC GACGC(5/10)
Hin1I 2129 GACGCC G[GA]^CG[CT]C
NaeI 917 GCCGGC GCC^GGC
NcoI 2569 CCATGG C^CATGG
NdeI 3426 CATATG CA^TATG
NgoMIV 917 GCCGGC G^CCGGC
NspI 2516 ACATGT [GA]CATG^[CT]
NspV 1363 TTCGAA TT^CGAA
PaeR7I 2392 CTCGAG C^TCGAG
PflFI 2550 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshBI 3467 ATTAAT AT^TAAT
SalI 2080 GTCGAC G^TCGAC
SbfI 1674 CCTGCAGG CCTGCA^GG
ScaI 2294 AGTACT AGT^ACT
SfuI 1363 TTCGAA TT^CGAA
SmaI 1079 CCCGGG CCC^GGG
SnaBI 1592 TACGTA TAC^GTA
Sse8387I 1674 CCTGCAGG CCTGCA^GG
Tsp509I 3412 AATT ^AATT
Tth111I 2550 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2392 CTCGAG C^TCGAG
XmaCI 1079 CCCGGG C^CCGGG
XmaI 1079 CCCGGG C^CCGGG
XmnI 2671 GAATATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 622 GTCTAC GT^[AC][GT]AC
2080 GTCGAC
AccIII 297 TCCGGA T^CCGGA
2913 TCCGGA
AflIII 2252 ACGTGT A^C[GA][CT]GT
2516 ACATGT
AocI 714 CCTGAGG CC^T[ACGT]AGG
1194 CCTCAGG
ApaI 551 GGGCCC GGGCC^C
739 GGGCCC
BalI 890 TGGCCA TGG^CCA
1613 TGGCCA
BglI 2390 GCCTCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
2422 GCCACCCTGGC
BsaAI 829 TACGTG [CT]AC^GT[GA]
1592 TACGTA
BseAI 297 TCCGGA T^CCGGA
2913 TCCGGA
Bsp1407I 689 TGTACA T^GTACA
3046 TGTACA
BspEI 297 TCCGGA T^CCGGA
2913 TCCGGA
BspHI 402 TCATGA T^CATGA
1926 TCATGA
BsrFI 98 ACCGGC [GA]^CCGG[CT]
917 GCCGGC
BsrGI 689 TGTACA T^GTACA
3046 TGTACA
BstAPI 3104 GCAGGCAATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
3237 GCACTTGGTGC
Bsu36I 714 CCTGAGG CC^T[ACGT]AGG
1194 CCTCAGG
Cfr10I 98 ACCGGC [GA]^CCGG[CT]
917 GCCGGC
DsaI 2569 CCATGG C^C[GA][CT]GG
2874 CCACGG
Eco81I 714 CCTGAGG CC^T[ACGT]AGG
1194 CCTCAGG
HincII 1850 GTCAAC GT[CT]^[GA]AC
2080 GTCGAC
HindII 1850 GTCAAC GT[CT]^[GA]AC
2080 GTCGAC
MluNI 890 TGGCCA TGG^CCA
1613 TGGCCA
MroI 297 TCCGGA T^CCGGA
2913 TCCGGA
MscI 890 TGGCCA TGG^CCA
1613 TGGCCA
NheI 2688 GCTAGC G^CTAGC
2879 GCTAGC
PvuII 1780 CAGCTG CAG^CTG
2693 CAGCTG
RcaI 402 TCATGA T^CATGA
1926 TCATGA
SspBI 689 TGTACA T^GTACA
3046 TGTACA
SspI 2672 AATATT AAT^ATT
3451 AATATT
XbaI 396 TCTAGA T^CTAGA
3086 TCTAGA
XcmI 1130 CCACTAAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1287 CCAATCTCCTGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AspHI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaJI BsaWI BseRI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrDI BsrI BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII DraII EaeI
EarI EcoNI EcoO109I EcoRII EcoT14I
FauI Fnu4HI FokI HaeII HaeIII
HapII HhaI HinP1I HindIII HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PleI PpuMI PstI RsaI SacI
SapI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StuI StyI TaqI
TfiI Tru9I TseI Tsp45I TspRI
TthHB8I XhoII