##################################
# NO CUT ENZYMES ( 76 enzymes) #
##################################
Acc65I AccIII AclI AflII AgeI
AhdI AscI AseI AsnI Asp700I
Asp718I AspEI AviII BcgI BciVI
BfrI BsaBI BsaI BsaWI BseAI
BsiWI BspDI BspEI BspHI BssHII
Bst1107I BstBI BstXI BstZ17I ClaI
CpoI DraI EagI Eam1105I EclXI
Eco52I EcoRV EcoT22I FseI FspI
HpaI KpnI KspI MamI MfeI
MluI MroI MunI NdeI NotI
NruI NsiI NspV PacI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII ScaI SexAI
SfuI SgrAI SmaI SpeI SphI
SspI SwaI XbaI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 52 enzymes) #
###################################
AatII 1749 GACGTC GACGT^C
AflIII 2059 ACATGT A^C[GA][CT]GT
Alw44I 482 GTGCAC G^TGCAC
AocI 2826 CCTGAGG CC^T[ACGT]AGG
Aor51HI 2332 AGCGCT AGC^GCT
ApaI 2220 GGGCCC GGGCC^C
ApaLI 482 GTGCAC G^TGCAC
AvrII 2757 CCTAGG C^CTAGG
BamHI 2396 GGATCC G^GATCC
BbeI 2156 GGCGCC GGCGC^C
BbrPI 1728 CACGTG CAC^GTG
BclI 1126 TGATCA T^GATCA
BglII 2 AGATCT A^GATCT
BlnI 2757 CCTAGG C^CTAGG
BsmBI 1416 GAGACG CGTCTC(1/5)
BsmI 2957 GAATGC GAATGC(1/-1)
Bsp1407I 1520 TGTACA T^GTACA
BspLU11I 2059 ACATGT A^CATGT
BsrBI 693 GAGCGG CCGCTC(-3/-3)
BsrDI 104 CATTGC GCAATG(2/0)
BsrGI 1520 TGTACA T^GTACA
BssSI 1074 CACGAG CACGAG(-5/-1)
Bsu36I 2826 CCTGAGG CC^T[ACGT]AGG
DrdI 2550 GACCTGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 2332 AGCGCT AGC^GCT
Eco81I 2826 CCTGAGG CC^T[ACGT]AGG
EcoNI 2722 CCTCAACTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 1392 GAATTC G^AATTC
FbaI 1126 TGATCA T^GATCA
HincII 3022 GTCGAC GT[CT]^[GA]AC
HindII 3022 GTCGAC GT[CT]^[GA]AC
KasI 2156 GGCGCC G^GCGCC
MslI 1000 CACGGGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 2156 GGCGCC GG^CGCC
NcoI 1762 CCATGG C^CATGG
NheI 1056 GCTAGC G^CTAGC
PaeR7I 66 CTCGAG C^TCGAG
PflMI 2426 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 1728 CACGTG CAC^GTG
PmlI 1728 CACGTG CAC^GTG
PpuMI 1213 GGGACCC [GA]G^G[AT]CC[CT]
SalI 3022 GTCGAC G^TCGAC
SapI 1223 GCTCTTC GCTCTTC(1/4)
SbfI 1634 CCTGCAGG CCTGCA^GG
SfoI 2156 GGCGCC GGC^GCC
SnaBI 3054 TACGTA TAC^GTA
Sse8387I 1634 CCTGCAGG CCTGCA^GG
SspBI 1520 TGTACA T^GTACA
StuI 1873 AGGCCT AGG^CCT
TfiI 2297 GATTC G^A[AT]TC
Van91I 2426 CCAGGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 66 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AccI 1275 GTAGAC GT^[AC][GT]AC
3022 GTCGAC
AcsI 1233 AAATTC [GA]^AATT[CT]
1392 GAATTC
ApoI 1233 AAATTC [GA]^AATT[CT]
1392 GAATTC
AspI 711 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1824 GACTTTGTC
BbsI 1746 GAAGAC GAAGAC(2/6)
600 GTCTTC
BbvCI 2351 CCTCAGC CCTCAGC(-5/-2)
2832 GCTGAGG
BlpI 72 GCTGAGC GC^T[ACGT]AGC
1557 GCTGAGC
Bpu1102I 72 GCTGAGC GC^T[ACGT]AGC
1557 GCTGAGC
BpuAI 1746 GAAGAC GAAGAC(2/6)
600 GTCTTC
BsaAI 1728 CACGTG [CT]AC^GT[GA]
3054 TACGTA
BsiEI 464 CGACCG CG[GA][CT]^CG
2309 CGACCG
BstAPI 497 GCAGGTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1727 GCACGTGGTGC
BstEII 966 GGTGACC G^GT[ACGT]ACC
2731 GGTGACC
BstPI 966 GGTGACC G^GT[ACGT]ACC
2731 GGTGACC
BstYI 2 AGATCT [GA]^GATC[CT]
2396 GGATCC
CelII 72 GCTGAGC GC^T[ACGT]AGC
1557 GCTGAGC
EcoO65I 966 GGTGACC G^GT[ACGT]ACC
2731 GGTGACC
HindIII 2145 AAGCTT A^AGCTT
2491 AAGCTT
MflI 2 AGATCT [GA]^GATC[CT]
2396 GGATCC
NaeI 876 GCCGGC GCC^GGC
1300 GCCGGC
NgoMIV 876 GCCGGC G^CCGGC
1300 GCCGGC
PflFI 711 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1824 GACTTTGTC
SacI 933 GAGCTC GAGCT^C
1959 GAGCTC
SfiI 81 GGCCCCGATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
1766 GGCCTGCTTGGCC
Tth111I 711 GACACGGTC GAC[ACGT]^[ACGT][ACGT]GTC
1824 GACTTTGTC
XhoII 2 AGATCT [GA]^GATC[CT]
2396 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 104 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BalI BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaHI
BsaJI BseRI BsgI BsiHKAI BsiYI
BslI BsmAI BsmFI BsoBI Bsp1286I
BspMI BsrFI BsrI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI DpnII DraII DraIII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MluNI MnlI MscI MseI
MspA1I MspI MvaI MvnI MwoI
NciI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI