##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII AccIII AclI AcyI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AspI AviII BbeI BcgI
BciVI BclI BglII BsaBI BsaHI
BseAI BsiEI BsiWI BsmBI BsmI
BspDI BspEI BspHI BstBI BstEII
BstPI BstXI ClaI CpoI DraI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoRV FbaI FseI
FspI Hin1I HindIII HpaI KasI
MamI MfeI MroI MunI NarI
NcoI NdeI NotI NruI NspV
PacI PaeR7I PflFI PmeI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SalI SbfI ScaI SexAI SfiI
SfoI SfuI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 34 enzymes) #
###################################
Acc65I 838 GGTACC G^GTACC
AgeI 403 ACCGGT A^CCGGT
Asp700I 2124 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 838 GGTACC G^GTACC
BbrPI 1050 CACGTG CAC^GTG
BglI 388 GCCAGGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaWI 403 ACCGGT [AT]^CCGG[AT]
Bsp1407I 1305 TGTACA T^GTACA
BspLU11I 1616 ACATGT A^CATGT
BsrGI 1305 TGTACA T^GTACA
BssHII 185 GCGCGC G^CGCGC
BssSI 801 CTCGTG CACGAG(-5/-1)
Bst1107I 2444 GTATAC GTA^TAC
BstZ17I 2444 GTATAC GTA^TAC
DraIII 1656 CACTACGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 334 GACCGCTGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoT22I 1225 ATGCAT ATGCA^T
HaeII 138 GGCGCT [GA]GCGC^[CT]
HincII 1946 GTTGAC GT[CT]^[GA]AC
HindII 1946 GTTGAC GT[CT]^[GA]AC
KpnI 838 GGTACC GGTAC^C
KspI 54 CCGCGG CCGC^GG
NsiI 1225 ATGCAT ATGCA^T
PinAI 403 ACCGGT A^CCGGT
PmaCI 1050 CACGTG CAC^GTG
PmlI 1050 CACGTG CAC^GTG
SacI 1752 GAGCTC GAGCT^C
SacII 54 CCGCGG CCGC^GG
SapI 993 GCTCTTC GCTCTTC(1/4)
SgrAI 180 CGCCGGCG C[GA]^CCGG[CT]G
SnaBI 2158 TACGTA TAC^GTA
SspBI 1305 TGTACA T^GTACA
XcmI 1337 CCAGCAGCAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 2124 GAACTGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 806 GAATTT [GA]^AATT[CT]
2761 GAATTT
AflII 465 CTTAAG C^TTAAG
520 CTTAAG
Alw44I 1045 GTGCAC G^TGCAC
2628 GTGCAC
AocI 1646 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
ApaLI 1045 GTGCAC G^TGCAC
2628 GTGCAC
ApoI 806 GAATTT [GA]^AATT[CT]
2761 GAATTT
AvrII 217 CCTAGG C^CTAGG
618 CCTAGG
BalI 1456 TGGCCA TGG^CCA
2208 TGGCCA
BamHI 302 GGATCC G^GATCC
1277 GGATCC
BanII 1112 GAGCCC G[GA]GC[CT]^C
1752 GAGCTC
BfrI 465 CTTAAG C^TTAAG
520 CTTAAG
BlnI 217 CCTAGG C^CTAGG
618 CCTAGG
BseRI 833 GAGGAG GAGGAG(10/8)
2250 GAGGAG
BsrBI 45 GAGCGG CCGCTC(-3/-3)
1126 GAGCGG
BsrDI 1228 CATTGC GCAATG(2/0)
1331 CATTGC
Bsu36I 1646 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
Eco81I 1646 CCTGAGG CC^T[ACGT]AGG
1857 CCTGAGG
EcoNI 1116 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1430 CCTTCGTGAGG
MluI 512 ACGCGT A^CGCGT
919 ACGCGT
MluNI 1456 TGGCCA TGG^CCA
2208 TGGCCA
MscI 1456 TGGCCA TGG^CCA
2208 TGGCCA
MslI 900 CACACTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1289 CATGAGCATG
NaeI 181 GCCGGC GCC^GGC
2341 GCCGGC
NgoMIV 181 GCCGGC G^CCGGC
2341 GCCGGC
NheI 1641 GCTAGC G^CTAGC
2345 GCTAGC
PflMI 2030 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2491 CCAGTATCTGG
Van91I 2030 CCAGCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2491 CCAGTATCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 113 enzymes) #
#########################################
AccI AccII AciI AfaI AflIII
AluI AlwI AlwNI AspHI AvaI
AvaII BanI BbsI BbvCI BbvI
BcnI BfaI BlpI BmyI BpmI
Bpu1102I BpuAI BsaAI BsaI BsaJI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrFI
BsrI BssKI BstAPI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI SphI
StuI StyI TaqI TfiI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
XhoII XmaCI XmaI