##################################
# NO CUT ENZYMES ( 84 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AocI Aor51HI AscI AseI AsnI
Asp718I AspI AvrII BcgI BclI
BfrI BglII BlnI BsiEI BsiWI
BsmBI BsmI BspDI BsrBI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I Bsu36I ClaI CpoI
DraI DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI KspI MfeI MunI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI SfiI SfuI
SgrAI SnaBI SpeI Sse8387I SspI
SwaI Tth111I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 41 enzymes) #
###################################
AccIII 2241 TCCGGA T^CCGGA
AcsI 1756 GAATTC [GA]^AATT[CT]
AcyI 2976 GGCGCC G[GA]^CG[CT]C
Alw44I 734 GTGCAC G^TGCAC
ApaLI 734 GTGCAC G^TGCAC
ApoI 1756 GAATTC [GA]^AATT[CT]
Asp700I 2647 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 2637 TGCGCA TGC^GCA
BbeI 2976 GGCGCC GGCGC^C
BbrPI 1984 CACGTG CAC^GTG
BlpI 2573 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2573 GCTGAGC GC^T[ACGT]AGC
BsaHI 2976 GGCGCC G[GA]^CG[CT]C
BsaWI 2241 TCCGGA [AT]^CCGG[AT]
BseAI 2241 TCCGGA T^CCGGA
BspEI 2241 TCCGGA T^CCGGA
BspHI 87 TCATGA T^CATGA
BspLU11I 595 ACATGT A^CATGT
CelII 2573 GCTGAGC GC^T[ACGT]AGC
EcoRI 1756 GAATTC G^AATTC
FspI 2637 TGCGCA TGC^GCA
Hin1I 2976 GGCGCC G[GA]^CG[CT]C
HincII 1223 GTCAAC GT[CT]^[GA]AC
HindII 1223 GTCAAC GT[CT]^[GA]AC
KasI 2976 GGCGCC G^GCGCC
MluI 704 ACGCGT A^CGCGT
MroI 2241 TCCGGA T^CCGGA
NarI 2976 GGCGCC GG^CGCC
PmaCI 1984 CACGTG CAC^GTG
PmlI 1984 CACGTG CAC^GTG
PshAI 2104 GACGCTGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 2792 CAGCTG CAG^CTG
RcaI 87 TCATGA T^CATGA
ScaI 1350 AGTACT AGT^ACT
SfoI 2976 GGCGCC GGC^GCC
SmaI 1065 CCCGGG CCC^GGG
SphI 2889 GCATGC GCATG^C
StuI 907 AGGCCT AGG^CCT
XmaCI 1065 CCCGGG C^CCGGG
XmaI 1065 CCCGGG C^CCGGG
XmnI 2647 GAATCGCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2838 GTCTAC GT^[AC][GT]AC
3148 GTCTAC
AccII 218 CGCG CG^CG
705 CGCG
AhdI 1720 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2525 GACTACCTGTC
ApaI 2517 GGGCCC GGGCC^C
2673 GGGCCC
AspEI 1720 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2525 GACTACCTGTC
AvaI 1065 CCCGGG C^[CT]CG[GA]G
2520 CCCGAG
BamHI 535 GGATCC G^GATCC
1666 GGATCC
BbsI 334 GAAGAC GAAGAC(2/6)
1829 GAAGAC
BbvCI 398 GCTGAGG CCTCAGC(-5/-2)
1049 GCTGAGG
BciVI 1174 GGATAC GTATCC(6/5)
152 GTATCC
BpuAI 334 GAAGAC GAAGAC(2/6)
1829 GAAGAC
BsaAI 1585 CACGTA [CT]AC^GT[GA]
1984 CACGTG
BsaBI 2140 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2690 GATCGGTATC
BsaI 969 GAGACC GGTCTC(1/5)
1152 GAGACC
BsoBI 1065 CCCGGG C^[CT]CG[GA]G
2520 CCCGAG
Bsp1407I 1653 TGTACA T^GTACA
2700 TGTACA
BsrGI 1653 TGTACA T^GTACA
2700 TGTACA
BstUI 218 CGCG CG^CG
705 CGCG
DraIII 1040 CACACCGTG CAC[ACGT][ACGT][ACGT]^GTG
2249 CACAAGGTG
Eam1105I 1720 GACCAAGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2525 GACTACCTGTC
MaeII 1586 ACGT A^CGT
1985 ACGT
MamI 2140 GATTCCCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2690 GATCGGTATC
MseI 1752 TTAA T^TAA
3087 TTAA
MvnI 218 CGCG CG^CG
705 CGCG
NaeI 1739 GCCGGC GCC^GGC
2202 GCCGGC
NgoMIV 1739 GCCGGC G^CCGGC
2202 GCCGGC
PleI 1622 GACTC GAGTC(4/5)
821 GAGTC
SapI 760 GAAGAGC GCTCTTC(1/4)
1033 GAAGAGC
SmlI 428 CTCAAG C^T[CT][GA]AG
587 CTCAAG
SspBI 1653 TGTACA T^GTACA
2700 TGTACA
Tru9I 1752 TTAA T^TAA
3087 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 102 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaII BalI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
Bsp1286I BspMI BsrDI BsrFI BsrI
BssKI BstAPI BstF5I BstNI BstYI
Cac8I CfoI Cfr10I Cfr13I DdeI
DpnII DraII DsaI EaeI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI HinP1I HinfI HpaII HphI
ItaI Ksp632I MaeI MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MwoI
NciI NcoI NdeII NlaIII NlaIV
NspI PflMI PstI RsaI SacI
Sau3AI Sau96I ScrFI SexAI SfaNI
SfcI StyI TaqI TfiI TseI
Tsp45I Tsp509I TspRI TthHB8I Van91I
XcmI XhoII