################################## # NO CUT ENZYMES ( 88 enzymes) # ################################## Acc65I AccIII AclI AgeI AocI AscI AseI AsnI Asp718I AspI AviII AvrII BamHI BcgI BciVI BclI BlnI BsaBI BseAI BsiWI BsmI Bsp1407I BspDI BspEI BspHI BspMI BsrDI BsrGI BssHII BssSI BstAPI BstBI BstXI Bsu36I ClaI CpoI DrdI EagI EclXI Eco52I Eco81I EcoNI EcoRV EcoT22I FbaI FseI FspI HincII HindII HpaI KpnI KspI MamI MfeI MluI MroI MunI NdeI NheI NotI NsiI NspV PacI PflFI PinAI PmeI PshAI PshBI Psp1406I PvuI RcaI RsrII SacII SalI SbfI ScaI SexAI SfiI SfuI SgrAI SnaBI SpeI SphI Sse8387I SspBI SwaI Tth111I XbaI ################################### # ONE CUT ENZYMES ( 48 enzymes) # ################################### AatII 950 GACGTC GACGT^C AfaI 483 GTAC GT^AC AflII 2474 CTTAAG C^TTAAG AflIII 1520 ACATGT A^C[GA][CT]GT AhdI 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Alw44I 1498 GTGCAC G^TGCAC Aor51HI 1823 AGCGCT AGC^GCT ApaI 847 GGGCCC GGGCC^C ApaLI 1498 GTGCAC G^TGCAC AspEI 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC BalI 1535 TGGCCA TGG^CCA BbrPI 27 CACGTG CAC^GTG BfaI 1079 CTAG C^TAG BfrI 2474 CTTAAG C^TTAAG BglI 1864 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC BglII 1320 AGATCT A^GATCT BlpI 1380 GCTCAGC GC^T[ACGT]AGC Bpu1102I 1380 GCTCAGC GC^T[ACGT]AGC BsaAI 27 CACGTG [CT]AC^GT[GA] BsaWI 1904 ACCGGA [AT]^CCGG[AT] BsiEI 342 CGACCG CG[GA][CT]^CG BspLU11I 1520 ACATGT A^CATGT Bst1107I 504 GTATAC GTA^TAC BstZ17I 504 GTATAC GTA^TAC CelII 1380 GCTCAGC GC^T[ACGT]AGC DraIII 1501 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG DsaI 2421 CCATGG C^C[GA][CT]GG EaeI 1535 TGGCCA [CT]^GGCC[GA] Eam1105I 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1823 AGCGCT AGC^GCT EcoRI 384 GAATTC G^AATTC HindIII 913 AAGCTT A^AGCTT MaeI 1079 CTAG C^TAG MluNI 1535 TGGCCA TGG^CCA MscI 1535 TGGCCA TGG^CCA NcoI 2421 CCATGG C^CATGG NruI 534 TCGCGA TCG^CGA PmaCI 27 CACGTG CAC^GTG PmlI 27 CACGTG CAC^GTG PstI 2551 CTGCAG CTGCA^G PvuII 2330 CAGCTG CAG^CTG RsaI 483 GTAC GT^AC SacI 2116 GAGCTC GAGCT^C SapI 1085 GCTCTTC GCTCTTC(1/4) SspI 2860 AATATT AAT^ATT StuI 1697 AGGCCT AGG^CCT TfiI 2291 GATTC G^A[AT]TC XcmI 2195 CCAGCTCACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG ################################### # TWO CUT ENZYMES ( 25 enzymes) # ################################### AccI 504 GTATAC GT^[AC][GT]AC 2211 GTAGAC Asp700I 103 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2697 GAAACATTTC BbeI 887 GGCGCC GGCGC^C 1716 GGCGCC BsgI 24 CTGCAC GTGCAG(16/14) 499 GTGCAG BstEII 2031 GGTCACC G^GT[ACGT]ACC 2537 GGTGACC BstPI 2031 GGTCACC G^GT[ACGT]ACC 2537 GGTGACC BstYI 1320 AGATCT [GA]^GATC[CT] 2171 AGATCC DraI 264 TTTAAA TTT^AAA 2298 TTTAAA EarI 1086 CTCTTC CTCTTC(1/4) 2216 CTCTTC EcoO65I 2031 GGTCACC G^GT[ACGT]ACC 2537 GGTGACC KasI 887 GGCGCC G^GCGCC 1716 GGCGCC Ksp632I 1086 CTCTTC CTCTTC(1/4) 2216 CTCTTC MflI 1320 AGATCT [GA]^GATC[CT] 2171 AGATCC MslI 837 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG 2034 CACCAGCGTG NaeI 1203 GCCGGC GCC^GGC 1725 GCCGGC NarI 887 GGCGCC GG^CGCC 1716 GGCGCC NgoMIV 1203 GCCGGC G^CCGGC 1725 GCCGGC NspI 249 ACATGC [GA]CATG^[CT] 1520 ACATGT SfaNI 150 GATGC GCATC(5/9) 2723 GCATC SfoI 887 GGCGCC GGC^GCC 1716 GGCGCC SmaI 1735 CCCGGG CCC^GGG 2176 CCCGGG XhoII 1320 AGATCT [GA]^GATC[CT] 2171 AGATCC XmaCI 1735 CCCGGG C^CCGGG 2176 CCCGGG XmaI 1735 CCCGGG C^CCGGG 2176 CCCGGG XmnI 103 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC 2697 GAAACATTTC ######################################### # 3 OR MORE CUT ENZYMES ( 97 enzymes) # ######################################### AccII AciI AcsI AcyI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BanI BanII BbsI BbvCI BbvI BcnI BmyI BpmI BpuAI BsaHI BsaI BsaJI BseRI BsiHKAI BsiYI BslI BsmAI BsmBI BsmFI BsoBI Bsp1286I BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI Cac8I CfoI Cfr10I Cfr13I DdeI DpnII DraII EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI MaeII MaeIII MboI MboII MnlI MseI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PaeR7I PflMI PleI PpuMI Sau3AI Sau96I ScrFI SfcI SmlI StyI TaqI Tru9I TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XhoI