##################################
# NO CUT ENZYMES ( 88 enzymes) #
##################################
Acc65I AccIII AclI AgeI AocI
AscI AseI AsnI Asp718I AspI
AviII AvrII BamHI BcgI BciVI
BclI BlnI BsaBI BseAI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspMI BsrDI BsrGI BssHII BssSI
BstAPI BstBI BstXI Bsu36I ClaI
CpoI DrdI EagI EclXI Eco52I
Eco81I EcoNI EcoRV EcoT22I FbaI
FseI FspI HincII HindII HpaI
KpnI KspI MamI MfeI MluI
MroI MunI NdeI NheI NotI
NsiI NspV PacI PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SbfI
ScaI SexAI SfiI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tth111I XbaI
###################################
# ONE CUT ENZYMES ( 48 enzymes) #
###################################
AatII 950 GACGTC GACGT^C
AfaI 483 GTAC GT^AC
AflII 2474 CTTAAG C^TTAAG
AflIII 1520 ACATGT A^C[GA][CT]GT
AhdI 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 1498 GTGCAC G^TGCAC
Aor51HI 1823 AGCGCT AGC^GCT
ApaI 847 GGGCCC GGGCC^C
ApaLI 1498 GTGCAC G^TGCAC
AspEI 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BalI 1535 TGGCCA TGG^CCA
BbrPI 27 CACGTG CAC^GTG
BfaI 1079 CTAG C^TAG
BfrI 2474 CTTAAG C^TTAAG
BglI 1864 GCCAAGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1320 AGATCT A^GATCT
BlpI 1380 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 1380 GCTCAGC GC^T[ACGT]AGC
BsaAI 27 CACGTG [CT]AC^GT[GA]
BsaWI 1904 ACCGGA [AT]^CCGG[AT]
BsiEI 342 CGACCG CG[GA][CT]^CG
BspLU11I 1520 ACATGT A^CATGT
Bst1107I 504 GTATAC GTA^TAC
BstZ17I 504 GTATAC GTA^TAC
CelII 1380 GCTCAGC GC^T[ACGT]AGC
DraIII 1501 CACAGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2421 CCATGG C^C[GA][CT]GG
EaeI 1535 TGGCCA [CT]^GGCC[GA]
Eam1105I 1178 GACCTCCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1823 AGCGCT AGC^GCT
EcoRI 384 GAATTC G^AATTC
HindIII 913 AAGCTT A^AGCTT
MaeI 1079 CTAG C^TAG
MluNI 1535 TGGCCA TGG^CCA
MscI 1535 TGGCCA TGG^CCA
NcoI 2421 CCATGG C^CATGG
NruI 534 TCGCGA TCG^CGA
PmaCI 27 CACGTG CAC^GTG
PmlI 27 CACGTG CAC^GTG
PstI 2551 CTGCAG CTGCA^G
PvuII 2330 CAGCTG CAG^CTG
RsaI 483 GTAC GT^AC
SacI 2116 GAGCTC GAGCT^C
SapI 1085 GCTCTTC GCTCTTC(1/4)
SspI 2860 AATATT AAT^ATT
StuI 1697 AGGCCT AGG^CCT
TfiI 2291 GATTC G^A[AT]TC
XcmI 2195 CCAGCTCACTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 504 GTATAC GT^[AC][GT]AC
2211 GTAGAC
Asp700I 103 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2697 GAAACATTTC
BbeI 887 GGCGCC GGCGC^C
1716 GGCGCC
BsgI 24 CTGCAC GTGCAG(16/14)
499 GTGCAG
BstEII 2031 GGTCACC G^GT[ACGT]ACC
2537 GGTGACC
BstPI 2031 GGTCACC G^GT[ACGT]ACC
2537 GGTGACC
BstYI 1320 AGATCT [GA]^GATC[CT]
2171 AGATCC
DraI 264 TTTAAA TTT^AAA
2298 TTTAAA
EarI 1086 CTCTTC CTCTTC(1/4)
2216 CTCTTC
EcoO65I 2031 GGTCACC G^GT[ACGT]ACC
2537 GGTGACC
KasI 887 GGCGCC G^GCGCC
1716 GGCGCC
Ksp632I 1086 CTCTTC CTCTTC(1/4)
2216 CTCTTC
MflI 1320 AGATCT [GA]^GATC[CT]
2171 AGATCC
MslI 837 CACCTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
2034 CACCAGCGTG
NaeI 1203 GCCGGC GCC^GGC
1725 GCCGGC
NarI 887 GGCGCC GG^CGCC
1716 GGCGCC
NgoMIV 1203 GCCGGC G^CCGGC
1725 GCCGGC
NspI 249 ACATGC [GA]CATG^[CT]
1520 ACATGT
SfaNI 150 GATGC GCATC(5/9)
2723 GCATC
SfoI 887 GGCGCC GGC^GCC
1716 GGCGCC
SmaI 1735 CCCGGG CCC^GGG
2176 CCCGGG
XhoII 1320 AGATCT [GA]^GATC[CT]
2171 AGATCC
XmaCI 1735 CCCGGG C^CCGGG
2176 CCCGGG
XmaI 1735 CCCGGG C^CCGGG
2176 CCCGGG
XmnI 103 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
2697 GAAACATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AccII AciI AcsI AcyI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BanI BanII BbsI BbvCI
BbvI BcnI BmyI BpmI BpuAI
BsaHI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BsrBI BsrFI BsrI
BssKI BstF5I BstNI BstUI Cac8I
CfoI Cfr10I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PaeR7I PflMI
PleI PpuMI Sau3AI Sau96I ScrFI
SfcI SmlI StyI TaqI Tru9I
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XhoI