################################## # NO CUT ENZYMES ( 95 enzymes) # ################################## AatII AccIII AclI AflII AflIII AgeI Alw44I ApaLI AscI AseI AsnI Asp700I AspI AviII BamHI BbeI BbrPI BciVI BfrI BglII BsaAI BsaBI BseAI BsiEI BsiWI BsmBI BsmI BspDI BspEI BspLU11I BsrFI BssHII BstBI BstEII BstPI Cfr10I ClaI CpoI DraI EagI EclXI Eco52I EcoO65I EcoRI EcoRV FseI FspI HpaI KasI KspI MamI MfeI MluI MroI MseI MunI NaeI NarI NcoI NdeI NgoMIV NotI NruI NspV PacI PaeR7I PflFI PinAI PmaCI PmeI PmlI PshBI Psp1406I PvuI RsrII SacII SalI SbfI ScaI SfiI SfoI SfuI SgrAI SmaI SnaBI SpeI Sse8387I SspI SwaI Tru9I Tth111I XhoI XmaCI XmaI XmnI ################################### # ONE CUT ENZYMES ( 43 enzymes) # ################################### Acc65I 1201 GGTACC G^GTACC AhdI 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AocI 3286 CCTAAGG CC^T[ACGT]AGG Aor51HI 1218 AGCGCT AGC^GCT ApaI 218 GGGCCC GGGCC^C Asp718I 1201 GGTACC G^GTACC AspEI 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AvrII 123 CCTAGG C^CTAGG BcgI 1369 GCATGGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BlnI 123 CCTAGG C^CTAGG BlpI 812 GCTCAGC GC^T[ACGT]AGC Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC Bsp1407I 2295 TGTACA T^GTACA BspHI 3159 TCATGA T^CATGA BspMI 3264 ACCTGC ACCTGC(4/8) BsrDI 2023 CATTGC GCAATG(2/0) BsrGI 2295 TGTACA T^GTACA Bst1107I 1079 GTATAC GTA^TAC BstYI 3181 GGATCT [GA]^GATC[CT] BstZ17I 1079 GTATAC GTA^TAC Bsu36I 3286 CCTAAGG CC^T[ACGT]AGG CelII 812 GCTCAGC GC^T[ACGT]AGC DrdI 3463 GACTGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC DsaI 525 CCGTGG C^C[GA][CT]GG Eam1105I 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC Eco47III 1218 AGCGCT AGC^GCT Eco81I 3286 CCTAAGG CC^T[ACGT]AGG EcoT22I 1577 ATGCAT ATGCA^T HincII 210 GTCAAC GT[CT]^[GA]AC HindII 210 GTCAAC GT[CT]^[GA]AC HindIII 1259 AAGCTT A^AGCTT KpnI 1201 GGTACC GGTAC^C MflI 3181 GGATCT [GA]^GATC[CT] NheI 1843 GCTAGC G^CTAGC NsiI 1577 ATGCAT ATGCA^T PshAI 2545 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC RcaI 3159 TCATGA T^CATGA SexAI 3279 ACCAGGT A^CC[AT]GGT SmlI 3052 CTTGAG C^T[CT][GA]AG SphI 1693 GCATGC GCATG^C SspBI 2295 TGTACA T^GTACA XbaI 1918 TCTAGA T^CTAGA XhoII 3181 GGATCT [GA]^GATC[CT] ################################### # TWO CUT ENZYMES ( 17 enzymes) # ################################### AccI 1079 GTATAC GT^[AC][GT]AC 2165 GTAGAC AcyI 758 GGCGTC G[GA]^CG[CT]C 2913 GGCGTC BbvCI 2032 CCTCAGC CCTCAGC(-5/-2) 1135 GCTGAGG BclI 1174 TGATCA T^GATCA 3162 TGATCA BglI 836 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC 1417 GCCCTTCTGGC BsaHI 758 GGCGTC G[GA]^CG[CT]C 2913 GGCGTC BsaWI 1204 ACCGGA [AT]^CCGG[AT] 2337 ACCGGA BsrBI 1350 CCGCTC CCGCTC(-3/-3) 2655 GAGCGG BstXI 2089 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 2248 CCAGGAGGCTGG EcoNI 2434 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG 2645 CCTGACTCAGG FbaI 1174 TGATCA T^GATCA 3162 TGATCA HgaI 759 GCGTC GACGC(5/10) 2914 GCGTC Hin1I 758 GGCGTC G[GA]^CG[CT]C 2913 GGCGTC MaeII 2584 ACGT A^CGT 2909 ACGT NspI 1693 GCATGC [GA]CATG^[CT] 2514 ACATGC PpuMI 1665 AGGACCT [GA]G^G[AT]CC[CT] 3282 AGGTCCT SapI 2713 GAAGAGC GCTCTTC(1/4) 156 GCTCTTC ######################################### # 3 OR MORE CUT ENZYMES ( 103 enzymes) # ######################################### AccII AciI AcsI AfaI AluI AlwI AlwNI ApoI AspHI AvaI AvaII BalI BanI BanII BbsI BbvI BcnI BfaI BmyI BpmI BpuAI BsaI BsaJI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BsrI BssKI BssSI BstAPI BstF5I BstNI BstUI Cac8I CfoI Cfr13I DdeI DpnII DraII DraIII EaeI EarI EcoO109I EcoRII EcoT14I FauI Fnu4HI FokI HaeII HaeIII HapII HhaI HinP1I HinfI HpaII HphI ItaI Ksp632I MaeI MaeIII MboI MboII MluNI MnlI MscI MslI MspA1I MspI MvaI MvnI MwoI NciI NdeII NlaIII NlaIV PflMI PleI PstI PvuII RsaI SacI Sau3AI Sau96I ScrFI SfaNI SfcI StuI StyI TaqI TfiI TseI Tsp45I Tsp509I TspRI TthHB8I Van91I XcmI