##################################
# NO CUT ENZYMES ( 95 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AgeI Alw44I ApaLI AscI AseI
AsnI Asp700I AspI AviII BamHI
BbeI BbrPI BciVI BfrI BglII
BsaAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BspDI BspEI BspLU11I
BsrFI BssHII BstBI BstEII BstPI
Cfr10I ClaI CpoI DraI EagI
EclXI Eco52I EcoO65I EcoRI EcoRV
FseI FspI HpaI KasI KspI
MamI MfeI MluI MroI MseI
MunI NaeI NarI NcoI NdeI
NgoMIV NotI NruI NspV PacI
PaeR7I PflFI PinAI PmaCI PmeI
PmlI PshBI Psp1406I PvuI RsrII
SacII SalI SbfI ScaI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
Tth111I XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 43 enzymes) #
###################################
Acc65I 1201 GGTACC G^GTACC
AhdI 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 3286 CCTAAGG CC^T[ACGT]AGG
Aor51HI 1218 AGCGCT AGC^GCT
ApaI 218 GGGCCC GGGCC^C
Asp718I 1201 GGTACC G^GTACC
AspEI 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 123 CCTAGG C^CTAGG
BcgI 1369 GCATGGTGCTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 123 CCTAGG C^CTAGG
BlpI 812 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 812 GCTCAGC GC^T[ACGT]AGC
Bsp1407I 2295 TGTACA T^GTACA
BspHI 3159 TCATGA T^CATGA
BspMI 3264 ACCTGC ACCTGC(4/8)
BsrDI 2023 CATTGC GCAATG(2/0)
BsrGI 2295 TGTACA T^GTACA
Bst1107I 1079 GTATAC GTA^TAC
BstYI 3181 GGATCT [GA]^GATC[CT]
BstZ17I 1079 GTATAC GTA^TAC
Bsu36I 3286 CCTAAGG CC^T[ACGT]AGG
CelII 812 GCTCAGC GC^T[ACGT]AGC
DrdI 3463 GACTGCTTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 525 CCGTGG C^C[GA][CT]GG
Eam1105I 3263 GACCTGCAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 1218 AGCGCT AGC^GCT
Eco81I 3286 CCTAAGG CC^T[ACGT]AGG
EcoT22I 1577 ATGCAT ATGCA^T
HincII 210 GTCAAC GT[CT]^[GA]AC
HindII 210 GTCAAC GT[CT]^[GA]AC
HindIII 1259 AAGCTT A^AGCTT
KpnI 1201 GGTACC GGTAC^C
MflI 3181 GGATCT [GA]^GATC[CT]
NheI 1843 GCTAGC G^CTAGC
NsiI 1577 ATGCAT ATGCA^T
PshAI 2545 GACGAGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 3159 TCATGA T^CATGA
SexAI 3279 ACCAGGT A^CC[AT]GGT
SmlI 3052 CTTGAG C^T[CT][GA]AG
SphI 1693 GCATGC GCATG^C
SspBI 2295 TGTACA T^GTACA
XbaI 1918 TCTAGA T^CTAGA
XhoII 3181 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AccI 1079 GTATAC GT^[AC][GT]AC
2165 GTAGAC
AcyI 758 GGCGTC G[GA]^CG[CT]C
2913 GGCGTC
BbvCI 2032 CCTCAGC CCTCAGC(-5/-2)
1135 GCTGAGG
BclI 1174 TGATCA T^GATCA
3162 TGATCA
BglI 836 GCCCAACTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
1417 GCCCTTCTGGC
BsaHI 758 GGCGTC G[GA]^CG[CT]C
2913 GGCGTC
BsaWI 1204 ACCGGA [AT]^CCGG[AT]
2337 ACCGGA
BsrBI 1350 CCGCTC CCGCTC(-3/-3)
2655 GAGCGG
BstXI 2089 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2248 CCAGGAGGCTGG
EcoNI 2434 CCTACCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
2645 CCTGACTCAGG
FbaI 1174 TGATCA T^GATCA
3162 TGATCA
HgaI 759 GCGTC GACGC(5/10)
2914 GCGTC
Hin1I 758 GGCGTC G[GA]^CG[CT]C
2913 GGCGTC
MaeII 2584 ACGT A^CGT
2909 ACGT
NspI 1693 GCATGC [GA]CATG^[CT]
2514 ACATGC
PpuMI 1665 AGGACCT [GA]G^G[AT]CC[CT]
3282 AGGTCCT
SapI 2713 GAAGAGC GCTCTTC(1/4)
156 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 103 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AspHI AvaI
AvaII BalI BanI BanII BbsI
BbvI BcnI BfaI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmAI BsmFI
BsoBI Bsp1286I BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DraIII EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeIII MboI MboII MluNI MnlI
MscI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV PflMI PleI PstI PvuII
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI StuI StyI TaqI
TfiI TseI Tsp45I Tsp509I TspRI
TthHB8I Van91I XcmI