##################################
# NO CUT ENZYMES ( 90 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AgeI AhdI AocI AscI AseI
AsnI Asp700I AspEI AspI BamHI
BbvCI BfrI BglII BsaBI BsaI
BsaWI BseAI BsiEI BsiWI BsmI
Bsp1407I BspDI BspEI BspHI BsrFI
BsrGI BssHII BstBI BstUI Bsu36I
Cfr10I ClaI CpoI DraI DrdI
EagI Eam1105I EclXI Eco52I Eco81I
EcoT22I FseI HincII HindII HpaI
KspI MamI MfeI MluI MroI
MunI MvnI NaeI NgoMIV NotI
NruI NsiI NspV PacI PflFI
PinAI PmeI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI SapI
ScaI SexAI SfiI SfuI SgrAI
SmaI SpeI SspBI SspI SwaI
Tth111I XbaI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccI 487 GTATAC GT^[AC][GT]AC
AcsI 3101 GAATTC [GA]^AATT[CT]
AcyI 992 GGCGCC G[GA]^CG[CT]C
Aor51HI 193 AGCGCT AGC^GCT
ApoI 3101 GAATTC [GA]^AATT[CT]
AviII 2821 TGCGCA TGC^GCA
BbeI 992 GGCGCC GGCGC^C
BbsI 2051 GTCTTC GAAGAC(2/6)
BclI 447 TGATCA T^GATCA
BglI 461 GCCTTTGGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 2051 GTCTTC GAAGAC(2/6)
BsaHI 992 GGCGCC G[GA]^CG[CT]C
BsgI 1068 CTGCAC GTGCAG(16/14)
BsmBI 77 GAGACG CGTCTC(1/5)
BspLU11I 2417 ACATGT A^CATGT
BsrDI 1265 CATTGC GCAATG(2/0)
BssSI 2758 CTCGTG CACGAG(-5/-1)
Bst1107I 487 GTATAC GTA^TAC
BstZ17I 487 GTATAC GTA^TAC
DraIII 403 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco47III 193 AGCGCT AGC^GCT
EcoRI 3101 GAATTC G^AATTC
EcoRV 772 GATATC GAT^ATC
FbaI 447 TGATCA T^GATCA
FspI 2821 TGCGCA TGC^GCA
Hin1I 992 GGCGCC G[GA]^CG[CT]C
KasI 992 GGCGCC G^GCGCC
NarI 992 GGCGCC GG^CGCC
NcoI 2 CCATGG C^CATGG
NheI 684 GCTAGC G^CTAGC
PaeR7I 2746 CTCGAG C^TCGAG
PshAI 1464 GACCCCAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 2143 GAGCTC GAGCT^C
SbfI 249 CCTGCAGG CCTGCA^GG
SfaNI 303 GCATC GCATC(5/9)
SfoI 992 GGCGCC GGC^GCC
SnaBI 961 TACGTA TAC^GTA
SphI 2818 GCATGC GCATG^C
Sse8387I 249 CCTGCAGG CCTGCA^GG
Tsp509I 3102 AATT ^AATT
XcmI 1846 CCAGAAGTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 2746 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
Acc65I 59 GGTACC G^GTACC
1631 GGTACC
ApaI 348 GGGCCC GGGCC^C
848 GGGCCC
Asp718I 59 GGTACC G^GTACC
1631 GGTACC
AvaI 1703 CTCGGG C^[CT]CG[GA]G
2746 CTCGAG
AvrII 1282 CCTAGG C^CTAGG
1898 CCTAGG
BcgI 15 CGACTTGTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
417 GCAAGAGCGTCG
BciVI 6 GGATAC GTATCC(6/5)
360 GTATCC
BlnI 1282 CCTAGG C^CTAGG
1898 CCTAGG
BlpI 196 GCTAAGC GC^T[ACGT]AGC
2780 GCTAAGC
Bpu1102I 196 GCTAAGC GC^T[ACGT]AGC
2780 GCTAAGC
BsoBI 1703 CTCGGG C^[CT]CG[GA]G
2746 CTCGAG
BstAPI 2730 GCAGCGACTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2824 GCAGTGTGTGC
BstEII 718 GGTGACC G^GT[ACGT]ACC
1461 GGTGACC
BstPI 718 GGTGACC G^GT[ACGT]ACC
1461 GGTGACC
BstYI 654 AGATCC [GA]^GATC[CT]
900 AGATCC
CelII 196 GCTAAGC GC^T[ACGT]AGC
2780 GCTAAGC
EarI 2614 GAAGAG CTCTTC(1/4)
2719 GAAGAG
EcoO65I 718 GGTGACC G^GT[ACGT]ACC
1461 GGTGACC
HindIII 1625 AAGCTT A^AGCTT
3095 AAGCTT
KpnI 59 GGTACC GGTAC^C
1631 GGTACC
Ksp632I 2614 GAAGAG CTCTTC(1/4)
2719 GAAGAG
MflI 654 AGATCC [GA]^GATC[CT]
900 AGATCC
MseI 1299 TTAA T^TAA
1649 TTAA
NdeI 1314 CATATG CA^TATG
1801 CATATG
TaqI 2666 TCGA T^CGA
2747 TCGA
Tru9I 1299 TTAA T^TAA
1649 TTAA
TthHB8I 2666 TCGA T^CGA
2747 TCGA
XhoII 654 AGATCC [GA]^GATC[CT]
900 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AfaI AflIII AluI Alw44I
AlwI AlwNI ApaLI AspHI AvaII
BalI BanI BanII BbrPI BbvI
BcnI BfaI BmyI BpmI BsaAI
BsaJI BseRI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrI BssKI BstF5I BstNI BstXI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EaeI EcoNI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
MaeI MaeII MaeIII MboI MboII
MluNI MnlI MscI MslI MspA1I
MspI MvaI MwoI NciI NdeII
NlaIII NlaIV NspI PflMI PleI
PmaCI PmlI PpuMI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfcI
SmlI StuI StyI TfiI TseI
Tsp45I TspRI Van91I