################################## # NO CUT ENZYMES ( 71 enzymes) # ################################## AatII Acc65I AclI AflII AflIII AgeI AocI Aor51HI AscI AseI AsnI Asp718I AvrII BfrI BlnI BsaBI BsiWI BsmI Bsp1407I BspDI BspLU11I BsrDI BsrGI BssHII Bst1107I BstBI BstZ17I Bsu36I ClaI CpoI DraI DraIII DrdI Eco47III Eco81I EcoRI EcoRV EcoT22I FseI HpaI KpnI KspI MamI MfeI MluI MunI NdeI NheI NotI NruI NsiI NspV PacI PaeR7I PinAI PmeI PshAI PshBI Psp1406I RsrII SacII SapI SexAI SfuI SgrAI SnaBI SpeI SspBI SspI SwaI XhoI ################################### # ONE CUT ENZYMES ( 35 enzymes) # ################################### AcsI 2723 GAATTT [GA]^AATT[CT] AhdI 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC ApoI 2723 GAATTT [GA]^AATT[CT] AspEI 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC AviII 1350 TGCGCA TGC^GCA BamHI 2333 GGATCC G^GATCC BbvCI 1562 GCTGAGG CCTCAGC(-5/-2) BcgI 919 CGAGGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10) BclI 2215 TGATCA T^GATCA BglII 1584 AGATCT A^GATCT BsaI 659 GAGACC GGTCTC(1/5) BsmBI 3051 CGTCTC CGTCTC(1/5) BspHI 2816 TCATGA T^CATGA BstAPI 2560 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC BstXI 2892 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG DsaI 1389 CCATGG C^C[GA][CT]GG EagI 2168 CGGCCG C^GGCCG Eam1105I 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC EclXI 2168 CGGCCG C^GGCCG Eco52I 2168 CGGCCG C^GGCCG EcoNI 1516 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG FbaI 2215 TGATCA T^GATCA FspI 1350 TGCGCA TGC^GCA HindIII 2223 AAGCTT A^AGCTT NaeI 1701 GCCGGC GCC^GGC NcoI 1389 CCATGG C^CATGG NgoMIV 1701 GCCGGC G^CCGGC PvuI 1480 CGATCG CGAT^CG RcaI 2816 TCATGA T^CATGA SbfI 1081 CCTGCAGG CCTGCA^GG ScaI 2545 AGTACT AGT^ACT SfiI 2507 GGCCCAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC Sse8387I 1081 CCTGCAGG CCTGCA^GG Tsp509I 2724 AATT ^AATT XbaI 1621 TCTAGA T^CTAGA ################################### # TWO CUT ENZYMES ( 30 enzymes) # ################################### AccIII 744 TCCGGA T^CCGGA 786 TCCGGA AspI 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2764 GACTCTGTC BalI 2208 TGGCCA TGG^CCA 2691 TGGCCA BseAI 744 TCCGGA T^CCGGA 786 TCCGGA BsiEI 1480 CGATCG CG[GA][CT]^CG 2168 CGGCCG BspEI 744 TCCGGA T^CCGGA 786 TCCGGA BssSI 1256 CTCGTG CACGAG(-5/-1) 1328 CTCGTG BstEII 16 GGTCACC G^GT[ACGT]ACC 3073 GGTCACC BstPI 16 GGTCACC G^GT[ACGT]ACC 3073 GGTCACC EcoO65I 16 GGTCACC G^GT[ACGT]ACC 3073 GGTCACC EcoT14I 736 CCTTGG C^C[AT][AT]GG 1389 CCATGG HincII 903 GTCGAC GT[CT]^[GA]AC 1052 GTCGAC HindII 903 GTCGAC GT[CT]^[GA]AC 1052 GTCGAC MluNI 2208 TGGCCA TGG^CCA 2691 TGGCCA MroI 744 TCCGGA T^CCGGA 786 TCCGGA MscI 2208 TGGCCA TGG^CCA 2691 TGGCCA PflFI 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2764 GACTCTGTC PflMI 223 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1384 CCAGCCCATGG SacI 304 GAGCTC GAGCT^C 1451 GAGCTC SalI 903 GTCGAC G^TCGAC 1052 GTCGAC SmaI 615 CCCGGG CCC^GGG 1059 CCCGGG SmlI 1898 CTCAAG C^T[CT][GA]AG 2114 CTCAAG SphI 289 GCATGC GCATG^C 1353 GCATGC StyI 736 CCTTGG C^C[AT][AT]GG 1389 CCATGG TfiI 805 GAATC G^A[AT]TC 1846 GATTC Tth111I 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC 2764 GACTCTGTC Van91I 223 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG 1384 CCAGCCCATGG XcmI 528 CCAAGCGGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG 2694 CCATCAGACAACTGG XmaCI 615 CCCGGG C^CCGGG 1059 CCCGGG XmaI 615 CCCGGG C^CCGGG 1059 CCCGGG ######################################### # 3 OR MORE CUT ENZYMES ( 122 enzymes) # ######################################### AccI AccII AciI AcyI AfaI AluI Alw44I AlwI AlwNI ApaI ApaLI Asp700I AspHI AvaI AvaII BanI BanII BbeI BbrPI BbsI BbvI BciVI BcnI BfaI BglI BlpI BmyI BpmI Bpu1102I BpuAI BsaAI BsaHI BsaJI BsaWI BseRI BsgI BsiHKAI BsiYI BslI BsmAI BsmFI BsoBI Bsp1286I BspMI BsrBI BsrFI BsrI BssKI BstF5I BstNI BstUI BstYI Cac8I CelII CfoI Cfr10I Cfr13I DdeI DpnII DraII EaeI EarI EcoO109I EcoRII FauI Fnu4HI FokI HaeII HaeIII HapII HgaI HhaI Hin1I HinP1I HinfI HpaII HphI ItaI KasI Ksp632I MaeI MaeII MaeIII MboI MboII MflI MnlI MseI MslI MspA1I MspI MvaI MvnI MwoI NarI NciI NdeII NlaIII NlaIV NspI PleI PmaCI PmlI PpuMI PstI PvuII RsaI Sau3AI Sau96I ScrFI SfaNI SfcI SfoI StuI TaqI Tru9I TseI Tsp45I TspRI TthHB8I XhoII XmnI