##################################
# NO CUT ENZYMES (   71 enzymes) #
##################################

AatII       Acc65I      AclI        AflII       AflIII      
AgeI        AocI        Aor51HI     AscI        AseI        
AsnI        Asp718I     AvrII       BfrI        BlnI        
BsaBI       BsiWI       BsmI        Bsp1407I    BspDI       
BspLU11I    BsrDI       BsrGI       BssHII      Bst1107I    
BstBI       BstZ17I     Bsu36I      ClaI        CpoI        
DraI        DraIII      DrdI        Eco47III    Eco81I      
EcoRI       EcoRV       EcoT22I     FseI        HpaI        
KpnI        KspI        MamI        MfeI        MluI        
MunI        NdeI        NheI        NotI        NruI        
NsiI        NspV        PacI        PaeR7I      PinAI       
PmeI        PshAI       PshBI       Psp1406I    RsrII       
SacII       SapI        SexAI       SfuI        SgrAI       
SnaBI       SpeI        SspBI       SspI        SwaI        
XhoI        


###################################
# ONE CUT ENZYMES (   35 enzymes) #
###################################

AcsI         2723  GAATTT               [GA]^AATT[CT]
AhdI          761  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI         2723  GAATTT               [GA]^AATT[CT]
AspEI         761  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII        1350  TGCGCA               TGC^GCA
BamHI        2333  GGATCC               G^GATCC
BbvCI        1562  GCTGAGG              CCTCAGC(-5/-2)
BcgI          919  CGAGGCTGATGC         (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI         2215  TGATCA               T^GATCA
BglII        1584  AGATCT               A^GATCT
BsaI          659  GAGACC               GGTCTC(1/5)
BsmBI        3051  CGTCTC               CGTCTC(1/5)
BspHI        2816  TCATGA               T^CATGA
BstAPI       2560  GCAGATCCTGC          GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI        2892  CCAGAGGCCTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI         1389  CCATGG               C^C[GA][CT]GG
EagI         2168  CGGCCG               C^GGCCG
Eam1105I      761  GACAAGAAGTC          GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI        2168  CGGCCG               C^GGCCG
Eco52I       2168  CGGCCG               C^GGCCG
EcoNI        1516  CCTCATTGAGG          CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI         2215  TGATCA               T^GATCA
FspI         1350  TGCGCA               TGC^GCA
HindIII      2223  AAGCTT               A^AGCTT
NaeI         1701  GCCGGC               GCC^GGC
NcoI         1389  CCATGG               C^CATGG
NgoMIV       1701  GCCGGC               G^CCGGC
PvuI         1480  CGATCG               CGAT^CG
RcaI         2816  TCATGA               T^CATGA
SbfI         1081  CCTGCAGG             CCTGCA^GG
ScaI         2545  AGTACT               AGT^ACT
SfiI         2507  GGCCCAGAGGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I     1081  CCTGCAGG             CCTGCA^GG
Tsp509I      2724  AATT                 ^AATT
XbaI         1621  TCTAGA               T^CTAGA


###################################
# TWO CUT ENZYMES (   30 enzymes) #
###################################

AccIII        744  TCCGGA               T^CCGGA
              786  TCCGGA              
AspI         1046  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACTCTGTC           
BalI         2208  TGGCCA               TGG^CCA
             2691  TGGCCA              
BseAI         744  TCCGGA               T^CCGGA
              786  TCCGGA              
BsiEI        1480  CGATCG               CG[GA][CT]^CG
             2168  CGGCCG              
BspEI         744  TCCGGA               T^CCGGA
              786  TCCGGA              
BssSI        1256  CTCGTG               CACGAG(-5/-1)
             1328  CTCGTG              
BstEII         16  GGTCACC              G^GT[ACGT]ACC
             3073  GGTCACC             
BstPI          16  GGTCACC              G^GT[ACGT]ACC
             3073  GGTCACC             
EcoO65I        16  GGTCACC              G^GT[ACGT]ACC
             3073  GGTCACC             
EcoT14I       736  CCTTGG               C^C[AT][AT]GG
             1389  CCATGG              
HincII        903  GTCGAC               GT[CT]^[GA]AC
             1052  GTCGAC              
HindII        903  GTCGAC               GT[CT]^[GA]AC
             1052  GTCGAC              
MluNI        2208  TGGCCA               TGG^CCA
             2691  TGGCCA              
MroI          744  TCCGGA               T^CCGGA
              786  TCCGGA              
MscI         2208  TGGCCA               TGG^CCA
             2691  TGGCCA              
PflFI        1046  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACTCTGTC           
PflMI         223  CCACCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1384  CCAGCCCATGG         
SacI          304  GAGCTC               GAGCT^C
             1451  GAGCTC              
SalI          903  GTCGAC               G^TCGAC
             1052  GTCGAC              
SmaI          615  CCCGGG               CCC^GGG
             1059  CCCGGG              
SmlI         1898  CTCAAG               C^T[CT][GA]AG
             2114  CTCAAG              
SphI          289  GCATGC               GCATG^C
             1353  GCATGC              
StyI          736  CCTTGG               C^C[AT][AT]GG
             1389  CCATGG              
TfiI          805  GAATC                G^A[AT]TC
             1846  GATTC               
Tth111I      1046  GACAGCGTC            GAC[ACGT]^[ACGT][ACGT]GTC
             2764  GACTCTGTC           
Van91I        223  CCACCTCATGG          CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
             1384  CCAGCCCATGG         
XcmI          528  CCAAGCGGCTCATGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
             2694  CCATCAGACAACTGG     
XmaCI         615  CCCGGG               C^CCGGG
             1059  CCCGGG              
XmaI          615  CCCGGG               C^CCGGG
             1059  CCCGGG              


#########################################
# 3 OR MORE CUT ENZYMES (  122 enzymes) #
#########################################

AccI        AccII       AciI        AcyI        AfaI        
AluI        Alw44I      AlwI        AlwNI       ApaI        
ApaLI       Asp700I     AspHI       AvaI        AvaII       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvI        BciVI       BcnI        BfaI        BglI        
BlpI        BmyI        BpmI        Bpu1102I    BpuAI       
BsaAI       BsaHI       BsaJI       BsaWI       BseRI       
BsgI        BsiHKAI     BsiYI       BslI        BsmAI       
BsmFI       BsoBI       Bsp1286I    BspMI       BsrBI       
BsrFI       BsrI        BssKI       BstF5I      BstNI       
BstUI       BstYI       Cac8I       CelII       CfoI        
Cfr10I      Cfr13I      DdeI        DpnII       DraII       
EaeI        EarI        EcoO109I    EcoRII      FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        KasI        Ksp632I     
MaeI        MaeII       MaeIII      MboI        MboII       
MflI        MnlI        MseI        MslI        MspA1I      
MspI        MvaI        MvnI        MwoI        NarI        
NciI        NdeII       NlaIII      NlaIV       NspI        
PleI        PmaCI       PmlI        PpuMI       PstI        
PvuII       RsaI        Sau3AI      Sau96I      ScrFI       
SfaNI       SfcI        SfoI        StuI        TaqI        
Tru9I       TseI        Tsp45I      TspRI       TthHB8I     
XhoII       XmnI