##################################
# NO CUT ENZYMES ( 71 enzymes) #
##################################
AatII Acc65I AclI AflII AflIII
AgeI AocI Aor51HI AscI AseI
AsnI Asp718I AvrII BfrI BlnI
BsaBI BsiWI BsmI Bsp1407I BspDI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstBI BstZ17I Bsu36I ClaI CpoI
DraI DraIII DrdI Eco47III Eco81I
EcoRI EcoRV EcoT22I FseI HpaI
KpnI KspI MamI MfeI MluI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmeI PshAI PshBI Psp1406I RsrII
SacII SapI SexAI SfuI SgrAI
SnaBI SpeI SspBI SspI SwaI
XhoI
###################################
# ONE CUT ENZYMES ( 35 enzymes) #
###################################
AcsI 2723 GAATTT [GA]^AATT[CT]
AhdI 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApoI 2723 GAATTT [GA]^AATT[CT]
AspEI 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AviII 1350 TGCGCA TGC^GCA
BamHI 2333 GGATCC G^GATCC
BbvCI 1562 GCTGAGG CCTCAGC(-5/-2)
BcgI 919 CGAGGCTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2215 TGATCA T^GATCA
BglII 1584 AGATCT A^GATCT
BsaI 659 GAGACC GGTCTC(1/5)
BsmBI 3051 CGTCTC CGTCTC(1/5)
BspHI 2816 TCATGA T^CATGA
BstAPI 2560 GCAGATCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 2892 CCAGAGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DsaI 1389 CCATGG C^C[GA][CT]GG
EagI 2168 CGGCCG C^GGCCG
Eam1105I 761 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EclXI 2168 CGGCCG C^GGCCG
Eco52I 2168 CGGCCG C^GGCCG
EcoNI 1516 CCTCATTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FbaI 2215 TGATCA T^GATCA
FspI 1350 TGCGCA TGC^GCA
HindIII 2223 AAGCTT A^AGCTT
NaeI 1701 GCCGGC GCC^GGC
NcoI 1389 CCATGG C^CATGG
NgoMIV 1701 GCCGGC G^CCGGC
PvuI 1480 CGATCG CGAT^CG
RcaI 2816 TCATGA T^CATGA
SbfI 1081 CCTGCAGG CCTGCA^GG
ScaI 2545 AGTACT AGT^ACT
SfiI 2507 GGCCCAGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
Sse8387I 1081 CCTGCAGG CCTGCA^GG
Tsp509I 2724 AATT ^AATT
XbaI 1621 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AccIII 744 TCCGGA T^CCGGA
786 TCCGGA
AspI 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACTCTGTC
BalI 2208 TGGCCA TGG^CCA
2691 TGGCCA
BseAI 744 TCCGGA T^CCGGA
786 TCCGGA
BsiEI 1480 CGATCG CG[GA][CT]^CG
2168 CGGCCG
BspEI 744 TCCGGA T^CCGGA
786 TCCGGA
BssSI 1256 CTCGTG CACGAG(-5/-1)
1328 CTCGTG
BstEII 16 GGTCACC G^GT[ACGT]ACC
3073 GGTCACC
BstPI 16 GGTCACC G^GT[ACGT]ACC
3073 GGTCACC
EcoO65I 16 GGTCACC G^GT[ACGT]ACC
3073 GGTCACC
EcoT14I 736 CCTTGG C^C[AT][AT]GG
1389 CCATGG
HincII 903 GTCGAC GT[CT]^[GA]AC
1052 GTCGAC
HindII 903 GTCGAC GT[CT]^[GA]AC
1052 GTCGAC
MluNI 2208 TGGCCA TGG^CCA
2691 TGGCCA
MroI 744 TCCGGA T^CCGGA
786 TCCGGA
MscI 2208 TGGCCA TGG^CCA
2691 TGGCCA
PflFI 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACTCTGTC
PflMI 223 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1384 CCAGCCCATGG
SacI 304 GAGCTC GAGCT^C
1451 GAGCTC
SalI 903 GTCGAC G^TCGAC
1052 GTCGAC
SmaI 615 CCCGGG CCC^GGG
1059 CCCGGG
SmlI 1898 CTCAAG C^T[CT][GA]AG
2114 CTCAAG
SphI 289 GCATGC GCATG^C
1353 GCATGC
StyI 736 CCTTGG C^C[AT][AT]GG
1389 CCATGG
TfiI 805 GAATC G^A[AT]TC
1846 GATTC
Tth111I 1046 GACAGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2764 GACTCTGTC
Van91I 223 CCACCTCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1384 CCAGCCCATGG
XcmI 528 CCAAGCGGCTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
2694 CCATCAGACAACTGG
XmaCI 615 CCCGGG C^CCGGG
1059 CCCGGG
XmaI 615 CCCGGG C^CCGGG
1059 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 122 enzymes) #
#########################################
AccI AccII AciI AcyI AfaI
AluI Alw44I AlwI AlwNI ApaI
ApaLI Asp700I AspHI AvaI AvaII
BanI BanII BbeI BbrPI BbsI
BbvI BciVI BcnI BfaI BglI
BlpI BmyI BpmI Bpu1102I BpuAI
BsaAI BsaHI BsaJI BsaWI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI BstYI Cac8I CelII CfoI
Cfr10I Cfr13I DdeI DpnII DraII
EaeI EarI EcoO109I EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI KasI Ksp632I
MaeI MaeII MaeIII MboI MboII
MflI MnlI MseI MslI MspA1I
MspI MvaI MvnI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PleI PmaCI PmlI PpuMI PstI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SfoI StuI TaqI
Tru9I TseI Tsp45I TspRI TthHB8I
XhoII XmnI