##################################
# NO CUT ENZYMES ( 102 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AflII AflIII Alw44I Aor51HI ApaLI
ApoI AscI AseI AsnI Asp718I
AviII AvrII BbeI BbrPI BcgI
BclI BfrI BglII BlnI BsaAI
BsaBI BseAI BsiWI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrGI
BssHII BstAPI BstBI BstEII BstPI
ClaI CpoI DraI DraIII DrdI
Eco47III EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HincII HindII
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PmaCI PmeI PmlI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
SexAI SfiI SfoI SfuI SgrAI
SnaBI SpeI SphI Sse8387I SspBI
SwaI XhoI
###################################
# ONE CUT ENZYMES ( 45 enzymes) #
###################################
AccI 1242 GTATAC GT^[AC][GT]AC
AccII 38 CGCG CG^CG
AcyI 1106 GGCGTC G[GA]^CG[CT]C
AgeI 1169 ACCGGT A^CCGGT
ApaI 482 GGGCCC GGGCC^C
Asp700I 387 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 311 GTGCTC G[AT]GC[AT]^C
AspI 898 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 248 TGGCCA TGG^CCA
BamHI 759 GGATCC G^GATCC
BciVI 1156 GTATCC GTATCC(6/5)
BglI 2491 GCCTCCCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1106 GGCGTC G[GA]^CG[CT]C
BsaWI 1169 ACCGGT [AT]^CCGG[AT]
BsiEI 1680 CGGCCG CG[GA][CT]^CG
BsiHKAI 311 GTGCTC G[AT]GC[AT]^C
BspMI 1390 GCAGGT ACCTGC(4/8)
BsrBI 529 GAGCGG CCGCTC(-3/-3)
BsrDI 2398 GCAATG GCAATG(2/0)
BssSI 2408 CTCGTG CACGAG(-5/-1)
Bst1107I 1242 GTATAC GTA^TAC
BstUI 38 CGCG CG^CG
BstZ17I 1242 GTATAC GTA^TAC
DsaI 1056 CCGTGG C^C[GA][CT]GG
EagI 1680 CGGCCG C^GGCCG
EclXI 1680 CGGCCG C^GGCCG
Eco52I 1680 CGGCCG C^GGCCG
EcoNI 558 CCTGATGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
HgaI 1107 GCGTC GACGC(5/10)
Hin1I 1106 GGCGTC G[GA]^CG[CT]C
HindIII 496 AAGCTT A^AGCTT
MluNI 248 TGGCCA TGG^CCA
MscI 248 TGGCCA TGG^CCA
MvnI 38 CGCG CG^CG
PflFI 898 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 453 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1169 ACCGGT A^CCGGT
SmaI 1763 CCCGGG CCC^GGG
SspI 253 AATATT AAT^ATT
StuI 2541 AGGCCT AGG^CCT
Tth111I 898 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 453 CCAAGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 1763 CCCGGG C^CCGGG
XmaI 1763 CCCGGG C^CCGGG
XmnI 387 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AhdI 362 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
799 GACTCACTGTC
AspEI 362 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
799 GACTCACTGTC
BbvCI 2472 CCTCAGC CCTCAGC(-5/-2)
463 GCTGAGG
BsgI 777 CTGCAC GTGCAG(16/14)
2051 GTGCAG
BsmBI 1932 GAGACG CGTCTC(1/5)
2331 GAGACG
BsrFI 1169 ACCGGT [GA]^CCGG[CT]
1678 ACCGGC
Cfr10I 1169 ACCGGT [GA]^CCGG[CT]
1678 ACCGGC
Eam1105I 362 GACTTCGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
799 GACTCACTGTC
EarI 658 CTCTTC CTCTTC(1/4)
682 GAAGAG
FauI 1788 CCCGC CCCGC(4/6)
1554 GCGGG
HaeII 768 GGCGCT [GA]GCGC^[CT]
2282 GGCGCT
Ksp632I 658 CTCTTC CTCTTC(1/4)
682 GAAGAG
MspA1I 445 CAGCTG C[AC]G^C[GT]G
2189 CAGCTG
PshAI 1648 GACCTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1934 GACGACGGTC
PstI 940 CTGCAG CTGCA^G
1699 CTGCAG
PvuII 445 CAGCTG CAG^CTG
2189 CAGCTG
ScaI 224 AGTACT AGT^ACT
1356 AGTACT
XbaI 795 TCTAGA T^CTAGA
2178 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 93 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AocI AvaI AvaII BanI BanII
BbsI BbvI BcnI BfaI BlpI
BmyI BpmI Bpu1102I BpuAI BsaI
BsaJI BseRI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI BstXI BstYI Bsu36I
Cac8I CelII CfoI Cfr13I DdeI
DpnII DraII EaeI Eco81I EcoO109I
EcoRII EcoT14I Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeI MaeII MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PpuMI
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XcmI XhoII