##################################
# NO CUT ENZYMES ( 86 enzymes) #
##################################
Acc65I AccIII AflII AgeI AocI
Aor51HI AscI AseI AsnI Asp700I
Asp718I AviII AvrII BalI BamHI
BbeI BbrPI BciVI BfrI BglI
BlnI BsaBI BsaWI BseAI BsiEI
BsiWI BspEI BsrBI BssHII BssSI
Bst1107I BstBI BstZ17I Bsu36I CfoI
CpoI DraI EagI EclXI Eco47III
Eco52I Eco81I EcoRI EcoRV EcoT22I
FseI FspI HaeII HhaI HinP1I
KasI KpnI KspI MamI MluNI
MroI MscI NaeI NarI NdeI
NgoMIV NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshBI PvuI RsrII SacII SalI
SapI SbfI SfiI SfoI SfuI
SgrAI SpeI SphI Sse8387I SwaI
XmnI
###################################
# ONE CUT ENZYMES ( 46 enzymes) #
###################################
AatII 2735 GACGTC GACGT^C
AclI 3929 AACGTT AA^CGTT
AcsI 3799 GAATTT [GA]^AATT[CT]
AcyI 2735 GACGTC G[GA]^CG[CT]C
ApaI 1329 GGGCCC GGGCC^C
ApoI 3799 GAATTT [GA]^AATT[CT]
BbvCI 3235 CCTCAGC CCTCAGC(-5/-2)
BcgI 3363 CGAAGACAGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 2868 TGATCA T^GATCA
BlpI 2750 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2750 GCTGAGC GC^T[ACGT]AGC
BsaAI 995 TACGTA [CT]AC^GT[GA]
BsaHI 2735 GACGTC G[GA]^CG[CT]C
BsmI 3660 GCATTC GAATGC(1/-1)
Bsp1407I 2859 TGTACA T^GTACA
BspDI 976 ATCGAT AT^CGAT
BsrFI 2763 ACCGGC [GA]^CCGG[CT]
BsrGI 2859 TGTACA T^GTACA
BstEII 2060 GGTGACC G^GT[ACGT]ACC
BstPI 2060 GGTGACC G^GT[ACGT]ACC
CelII 2750 GCTGAGC GC^T[ACGT]AGC
Cfr10I 2763 ACCGGC [GA]^CCGG[CT]
ClaI 976 ATCGAT AT^CGAT
EcoNI 3283 CCTGAGTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 2060 GGTGACC G^GT[ACGT]ACC
FauI 3595 CCCGC CCCGC(4/6)
FbaI 2868 TGATCA T^GATCA
Hin1I 2735 GACGTC G[GA]^CG[CT]C
HincII 4023 GTTAAC GT[CT]^[GA]AC
HindII 4023 GTTAAC GT[CT]^[GA]AC
HindIII 1174 AAGCTT A^AGCTT
HpaI 4023 GTTAAC GTT^AAC
MfeI 915 CAATTG C^AATTG
MluI 1885 ACGCGT A^CGCGT
MunI 915 CAATTG C^AATTG
NheI 3969 GCTAGC G^CTAGC
PshAI 2645 GACAGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 3929 AACGTT AA^CGTT
PstI 1947 CTGCAG CTGCA^G
SacI 795 GAGCTC GAGCT^C
SexAI 1482 ACCTGGT A^CC[AT]GGT
SnaBI 995 TACGTA TAC^GTA
SspBI 2859 TGTACA T^GTACA
SspI 1806 AATATT AAT^ATT
StuI 930 AGGCCT AGG^CCT
XbaI 2095 TCTAGA T^CTAGA
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AccI 1718 GTCTAC GT^[AC][GT]AC
3898 GTCTAC
AccII 1886 CGCG CG^CG
2854 CGCG
AhdI 3522 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3814 GACACATAGTC
Alw44I 392 GTGCAC G^TGCAC
1686 GTGCAC
ApaLI 392 GTGCAC G^TGCAC
1686 GTGCAC
AspEI 3522 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3814 GACACATAGTC
AspI 1481 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2470 GACGGTGTC
BglII 1056 AGATCT A^GATCT
3618 AGATCT
BsmBI 2737 CGTCTC CGTCTC(1/5)
2976 GAGACG
BspHI 57 TCATGA T^CATGA
2035 TCATGA
BsrDI 970 CATTGC GCAATG(2/0)
3261 CATTGC
BstAPI 887 GCAGAGGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2611 GCACTCCCTGC
BstUI 1886 CGCG CG^CG
2854 CGCG
BstXI 241 CCAAATAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1284 CCACCGCACTGG
DrdI 2393 GACCTTAGTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2978 GACGTGCCAGTC
EaeI 1866 TGGCCG [CT]^GGCC[GA]
2104 TGGCCG
Eam1105I 3522 GACAGTTTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
3814 GACACATAGTC
EarI 2247 CTCTTC CTCTTC(1/4)
2346 CTCTTC
HgaI 1265 GACGC GACGC(5/10)
1884 GACGC
Ksp632I 2247 CTCTTC CTCTTC(1/4)
2346 CTCTTC
MvnI 1886 CGCG CG^CG
2854 CGCG
PaeR7I 1738 CTCGAG C^TCGAG
2157 CTCGAG
PflFI 1481 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2470 GACGGTGTC
PflMI 1380 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3724 CCATTGTCTGG
RcaI 57 TCATGA T^CATGA
2035 TCATGA
ScaI 1094 AGTACT AGT^ACT
2072 AGTACT
Tth111I 1481 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
2470 GACGGTGTC
Van91I 1380 CCAGGCACTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3724 CCATTGTCTGG
XhoI 1738 CTCGAG C^TCGAG
2157 CTCGAG
#########################################
# 3 OR MORE CUT ENZYMES ( 97 enzymes) #
#########################################
AciI AfaI AflIII AluI AlwI
AlwNI AspHI AvaI AvaII BanI
BanII BbsI BbvI BcnI BfaI
BmyI BpmI BpuAI BsaI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspLU11I
BspMI BsrI BssKI BstF5I BstNI
BstYI Cac8I Cfr13I DdeI DpnII
DraII DraIII DsaI EcoO109I EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HinfI HpaII HphI ItaI MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MslI MspA1I MspI
MvaI MwoI NciI NcoI NdeII
NlaIII NlaIV NspI PleI PpuMI
PvuII RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmaI SmlI StyI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XcmI XhoII
XmaCI XmaI